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†Electronic Supplementary Information (ESI) Nano thick poly (ε-caprolactone)-poly (ethylene glycol) coatings developed by catalyst free plasma assisted copolymerization process for biomedical applications
Sudhir Bhatt*, Jérome Pulpytel, Massoud Mirshahi and Farzaneh Arefi-Khonsari*
Fig S1 Experimental Set up for plasma copolymerization
Fig S2 Flow rates of ε-CL and DEGME precursor molecules in the plasma reactor as a
function of carrier gas (Ar) flow rate at 0.5 mbar pressure.
Fig S3 FTIR-ATR spectra of ε-CL and DEGME liquid monomer (measurement volume was
40μL each)
Fig S4 FTIR-ATR spectra of PCL-co-PEG (1:4) coatings deposited at (a) 50W CW (b) 20W
CW (c) 0.25W PW and (d) 1W PW
Fig S5 FTIR-ATR spectra of pulsed plasma polymerized PCL, pDEGME and PCL-co-PEG
coatings deposited at Peff = 1W.
Fig S6 Enlargement of the MALDI-ToF mass spectrum (210-370 Da) of plasma polymerized
PCL-co-PEG coating prepared in alpha-matrix.
Fig S7 Enlargement of the MALDI-ToF mass spectrum (370-550 Da) of plasma polymerized
PCL-co-PEG coating prepared in alpha-matrix.
Fig S8 Microscopic images of HBMEC cell adhesion and proliferation on plasma
polymerized coatings for 24, 48 and 72 hrs of cell culture. Bare glass and PS plate were taken
positive control.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
Experimental set up for plasma copolymerization
Fig S1 Schematic diagram of the low pressure inductively excited RF tubular plasma reactor set-up for copolymerization.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
5 10 15 200,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
Pre
curs
sor
flo
w r
ate
at 0
.5 m
bar
pre
ssu
re (
sccm
)
Ar flow rate (sccm)
ε-CL flow rate (sccm) DEGME flow rate (sccm)
Fig S2 Flow rates of ε-CL and DEGME precursor molecules in the plasma reactor as a function of carrier gas (Ar) flow rate at 0.5 mbar pressure.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
FTIR-ATR spectra of ε-CL and DEGME liquid monomer
600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 3400 3600 3800
0,7
0,8
0,9
1,0
1,1
1,2
2867cm-1
1107 cm-1
2946 cm-1
1729 cm-1
DEGME
ε-CL
Wavenumber (cm-1)
Tra
nsm
itta
nce
(a.
u)
Fig S3 FTIR-ATR spectra of ε-CL and DEGME monomer (measurement volume was
40μL each)
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
FTIR Spectra of plasma polymerized PCL-co-PEG coatings at different effective power
600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600
PCL-co-PEG(1:4) Peff
=50W CW
Tra
nsm
issi
on (
arb.
unit)
Wavenumber (cm-1)
2946 cm-1
2867 cm-1
600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600
2946 cm-1
2867 cm-1
PCL-co-PEG(1:4) Peff
=20W CW
Tra
nsm
issi
on (
arb.
unit)
Wavenumber (cm-1)
600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600
2946 cm-1
2867 cm-1
1724 cm-1
PCL-co-PEG(1:4) Peff
=0.25W PW
Tra
nsm
issi
on (
arb.
unit)
Wavenumber (cm-1)600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600
2867 cm-1 2946 cm-1
1724 cm-1
1107 cm-1
PCL-co-PEG(1:4) Peff
=1W PWT
rans
mis
sio
n (a
rb.u
nit)
Wavenumber (cm-1)
(a) (b)
(c) (d)
1246 cm-1
Fig S4 FTIR-ATR spectra of PCL-co-PEG (1:4) coatings deposited at (a) 50W CW (b) 20W CW (c) 0.25W PW and (d) 1W PW
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
Detailed FTIR-ATR Spectra of plasma polymerized coatings deposited at Peff=1W for different ε-CL:DEGME ratio
600 800 1000 1200 1400 1600 1800 2600 2800 3000 3200 3400 3600 380
0,95
1,00
1,05
1,10
1,15
1,20
Tra
nsm
issi
on
(au
)
Wavenumber (cm-1)
PCL
pDEGME
PCL-co-PEG (1:1)
PCL-co-PEG(1:2)
PCL-co-PEG(1:3)
PCL-co-PEG(1:4)
Fig S5 FTIR-ATR spectra of pulsed plasma polymerized PCL, pDEGME and PCL-co-PEG coatings deposited at Peff = 1W.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370
0
10
20
30
40
50
60
70
δm=32,1
δm=32
δm=32δm=44,1
δm=114δm=88
δm=88
260,2
273,3
270,2
300,2
307288,5
304,3
272,2
228,2
354,5
242,2 248,5
256,5
230,2
240,2219
No
rmal
ized
Inte
nsi
ty (
%)
Mass (m/z)
212,2
δm=44,3
Fig S6 Enlargement of the MALDI-ToF mass spectrum (210-370 Da) of plasma polymerized PCL-co-PEG coating prepared in alpha-matrix.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550
0
20
40
60
80
100
δm=44
443 488,7
Nor
mal
ized
Inte
nsity
(%
)
Mass (m/z)
379,1
456,3
457,3
442,3
434,3 458,3470,3 501,3
518,3 538,2
Fig S7 Enlargement of the MALDI-ToF mass spectrum (370-550 Da) of plasma polymerized PCL-co-PEG coating prepared in alpha-matrix.
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
HBMEC cell adhesion and proliferation on plasma polymerized coatings
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012
Fig S8 Microscopic images of HBMEC cell adhesion and proliferation on plasma polymerized coatings for 24, 48 and 72 hrs of cell culture. Bare glass and PS plate were taken as positive control (Optical Magnification: 10X)
Electronic Supplementary Material (ESI) for RSC AdvancesThis journal is © The Royal Society of Chemistry 2012