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Tzu Chi Med J 2004 � 16 � No. 2NOQ �� !

Immunohistochemical Evaluation of the NM23-H1 Gene Product in the

Oral Squamous Cell Carcinoma

Tze-Ta Huang, Jeh-En Tzeng1, Young-Kie Wang2

Department of Oral and Maxillofacial Surgery, Pathology1, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan;

Department of Oral and Maxillofacial Surgery2, Taichung Veterans General Hospital, Taichung, Taiwan

ABSTRACTObjective: To evaluate the relationship between the MN23-H1 gene product and the disease of oral squamous cellcarcinoma. Materials and Methods: Fourty-six subjects who had received tumor ablation surgery and had completemedical records of the oral squamous cell carcinoma (male/female: 43/3; age: 33-88 years old, average, 54.32 yearsold; buccal mucosa, 22 cases; tongue, 13 cases; and gingival, 11 cases) were selected from 2000 to 2002 in BuddhistDalin Tzu Chi General Hospital, Taiwan. Expressions of the NM23-H1 gene in these tumor cells were analyzedimmunohistochemically. Results: The results show that the expression of the NM23-H1 gene was slightly increased inthese 46 cases. There were 25 cases (54.3%) which exhibited positive staining. After analyzing these data with X2

analysis, expression of the NM23-H1 gene was not statistically related to gender (male or female), site (buccal mucosa,tongue, or gingiva), tumor differentiation (well or non-well), neck lymph nodes metastasis (yes or no), or distantmetastasis (yes or no) (p > 0.05). Conclusions: This study shows that expression of the NM23-H1 gene was slightlyincreased in oral squamous cell carcinoma, but failed to show a clinically, the predictable relationship between necklymph node metastasis and NM23-H1 gene expression. (Tzu Chi Med J 2004; 16:119-124)

Key words: NM23 gene, metastasis suppressive gene, oral cancer, immunohistochemistry

Received: July 3, 2003, Revised: August 1, 2003, Accepted: August 29, 2003Address reprint requests and correspondence to: Dr. Tze-Ta Huang, Department of Oral and Maxillofacial Surgery, BuddhistDalin Tzu Chi General Hospital, 2, Min Sheng Road, Dalin, Chiayi, Taiwan