Óragének (és a melatonin) Óragének (és a melatonin)

Rékási Zoltán Rékási Zoltán

PTE PTE ÁOK ÁOK Anatómiai IntézetAnatómiai Intézet

2

3

4

birds mammals

5

6

7

8

5 % CO2 +95 % AIR

PERISTALTICPUMP

FRACTION COLLECTOR

FOR SAMPLEDILUTION

37 Co

WATERBATH

AIRW ITHDRAWAL SAMPLE

MEDIUMSAMPLE

CE

LL

S +

SE

PH

AD

EX

WATERJACKET

C s.V .

9

Melatonin elválasztás explantált patkány tobozmirigyből normál in vitro megvilágítási ciklusban

10

0

10

20

30

40

50

18 0 6 12 18 0 6 12 18 0 6 12 18 0 6hours

Melatonin elválasztás explantált csirke tobozmirigybőlnormál in vitro megvilágítási ciklusban

Melatonin elválasztás explantált csirke tobozmirigybőlnormál in vitro megvilágítási ciklusban

11

CH2CH2 NH2HO

NH

NH CH3

OCCH2CH2HO

NH

NH CH3

OCCH2CH2O

NH

CH3

SEROTONIN

N-ACETYLSEROTONIN

MELATONIN

N-acetyltransferase (AA-NAT)

hydroxyindol-o-methyltransferase (HIOMT) SAdM

AcCoA

MELATONIN SYNTHESIS

LIGHT

MELATONIN RELEASE

SIGNAL TRANSDUCTION

Adrenergic and VPAC-RECEPTORS

NORADRENERGIC and PEPTIDERGIC PATHWAYS

12

CH2CH2 NH2HO

NH

NH CH3

OCCH2CH2HO

NH

NH CH3

OCCH2CH2O

NH

CH3

SEROTONIN

N-ACETYLSEROTONIN

MELATONIN

N-acetyltransferase (AA-NAT)

hydroxyindol-o-methyltransferase (HIOMT) SAdM

AcCoA

PHOTORECEPTORS

CLOCK (PACEMAKER) Clock-genes

MELATONIN SYNTHESIS

LIGHT

MELATONIN RELEASE

? ?

?

SIGNAL TRANSDUCTION

13

14

period timeless

TIMPER

Clock

CLOCK BMAL1

Bmal1

Activatio

nInh

ibit

ion

time

gen

e ex

pre

ssio

n

Four-gene core transcription-translation loopFour-gene core transcription-translation loop

DELAY

15

16

Clock-genes in Drosophila and mammals Clock-genes in Drosophila and mammals PERIOD1

PERIOD2

PERIOD3

NLS bHLH/PAS

PERIOD

TIMELESS

NLS NLS NLS

CLOCK

CYCLE BMAL1

Doubletime casein kinase I epsilon

Cryptochrome2

CryptochromeCryptochrome1

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19

Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland

Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken pineal gland

20

Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina

Expression pattern of cAANAT, cBmal1 and cClock mRNA in chicken retina

21

Expression of retinal and pineal cAANAT mRNA in chicken housed

in constant darkness for 1 day or 1 week

Expression of retinal and pineal cAANAT mRNA in chicken housed

in constant darkness for 1 day or 1 week

retina pineal

22

Expression of retinal and pineal cBmal1 mRNA in chicken housed

in constant darkness for 1 day or 1 week

Expression of retinal and pineal cBmal1 mRNA in chicken housed

in constant darkness for 1 day or 1 week

retina pineal

23

Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week

Expression of retinal and pineal cAANAT mRNA in chicken housed in constant light for 1 day or 1 week

retina pineal

24

retina pineal

Expression of retinal and pineal cBmal1 mRNA in chicken housed

in constant light for 1 day or 1 week

Expression of retinal and pineal cBmal1 mRNA in chicken housed

in constant light for 1 day or 1 week

25

Model of circadian clock-work within an individual retinal or pineal

photoreceptor cell

Model of circadian clock-work within an individual retinal or pineal

photoreceptor cell

cCry2

cCry1

cPer2

cPer3

cAANAT

Output/melatonin

Bmal1

Clock Resetting the clock by light

26