PAMC: Polar and Alpine Microbial Collec-tion

Yung Mi Lee1, Cheng-Dae Choe2, Jeong-Han Yim1, Hong Kum Lee1, and Soon Gyu Hong1

1 Korea Polar Research Institute 2 BRN Science Co.

Needs for Database

▣ Management and sharing of bio-resources from polar areas

▣ Sharing biodiversity information with national and international organization

www. iobis.org

Culture Collections

No. of Culture collections No. of Cultures

Total 585 1,725,498

755,818 of them are Bacteria.505,521 of them are Fungi....

www.wfcc.info

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PAMC: Polar and Alpine Microbial Collection

▣ pamc.kopri.re.kr

▣ Culture Collection to maintain and distribute microorganisms originated from polar areas

▣ Approximately 500 strains are available for delivery and the number will increase gradually

Polar and Alpine Microbial Col-lection

Polar and Alpine Microbial Collection

About PAMC

Data Management of PAMC

PAMCPAMC

CuratorsCurators

Public databasesPublic databases

Public usersPublic users

Search

Datadownload

Public users Contributors

Public users Contributors

Deposit of resources

Delivery of resources

• Long-term preservation of bio-resources• Identification• Characterization• Data updates

• Metadata upload (AMD)• Original data transfer (EBA, SCAR-Marbin etc)

• Data download (GenBank Taxonomy etc)

Strains

▣ Microbial cultures Bacteria: 6,400

strains

Fungi: 300 strains

Freezing using Glycerol (-180C) and DMSO (-80C)

Freeze-dry (4C)

▣ Preservation

Preservation of strains

84%

16%

Abiotic sample

Biotic sample

Phylum composition of identified strains

Gammaproteobacteria,597

Actinobacteria, 188

Bacteroidetes,191

Firmicutes, 265

Betaproteobacteria,49 Alphaproteobacteria,50

Habitats of strains

Summary of PAMC Strains

Locality distribution of strains

24%

67%

10%

Antarctica

Arctic

Other

Kara sea (40%) Svalbard (21%) Barents sea (3%) Russia (3%))

King George Island (23%) Adeli Island (0.5%)

Austria (Alps) (8%) Mongol (1%) Okhotsk sea (1%)

Sediment 43.70%Soil 20.50%Water 9.70%Cryoconite 8.50%Rock 1.10%Glacier/Snow 0.30%

Biofilm 8.50%Algae 3.60%Lichen 1.30%Debris (animal/plant)

1.20%

Animal 0.80%Moss 0.80%

Composition of Identified Strains

Actinobacte-ria 16 Genera 188 Strains

Bac-teroidetes 25 Genera 191 Strains

Firmicutes 11 Genera 265 Strains

Alphaproteobacte-ria 16 Genera 50 Strains

Betaproteobacte-ria 4 Genera 49 Strains

Gammaproteobac-teria 18 Genera 597 Strains

Physiological Characteristics

▲ Growth on the 96 well plate

▲ Formation of hydrolytic zone on the 96 well plate

Growth Temperature

No.

of

Str

ain

sN

o.

of

Str

ain

s

4℃ 10℃ 15℃ 20℃ 25℃ 30℃ 37℃0

500

1000

1500

2000

2500

3000

Score 1Score 2Score 3Score 4

ProteaseLipase 10℃ 20℃0

100

200

300

400

500

Exopolysaccha-ride

10℃ 20℃ 10℃ 20℃0

200

400

600

800

1000

Score 1Score 2Score 3Score 4

Sample Database

Strain Database

①

Pseudoalteromonas

②

④

v

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③

PAMC - Searching

Polar and Alpine Microbial Collection

Polar and Alpine Microbial Collection

PAMC – Advanced Searching

Searching Keywords in PAMC

Searching for microbial strains using various keywords such as taxonomy, ge-ography, habitats and physiological characteristics are available in PAMC.

Taxonomy Geography

Habitats Characteristics

Polar and Alpine Microbial Collection

Vision of PAMC

▣ To maintain strains for the sustainable use

▣ To supply authentic strains for polar sciences

▣ To expand the service materials: fungal culture, gDNA, metabo-lite extract, etc

▣ PAMC website will be available on 1 October

Thank you!