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PEMANFAATAN EKSTRAK ETANOL BUAH SOSIS (Kigelia africana (Linn.)

Benth.) SEBAGAI ANTIOKSIDAN ALAMI DAN ANTIBAKTERI DALAM

PEMBUATAN SABUN TRANSPARAN

UTILIZATION OF SAUSAGE FRUIT (Kigelia africana (Linn.) Benth.)

ETHANOL EXTRACT AS A NATURAL ANTIOXIDANT AND

ANTIBACTERIAL IN TRANSPARENT SOAP

Oleh,

Birgitta Eknis Putri

NIM: 652010006

TUGAS AKHIR

Diajukan kepada Program Studi: Kimia, Fakultas: Sains dan Matematika guna

memenuhi sebagian dari persyaratan untuk mencapai gelar Sarjana Sains (Kimia)

Program Studi Kimia

Fakultas Sains dan Matematika

Universitas Kristen Satya Wacana

Salatiga

2014

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PEMANFAATAN EKSTRAK ETANOL BUAH SOSIS (Kigelia africana (Linn.)

Benth.) SEBAGAI ANTIOKSIDAN ALAMI DAN ANTIBAKTERI DALAM

PEMBUATAN SABUN TRANSPARAN

UTILIZATION OF SAUSAGE FRUIT (Kigelia africana (Linn.)

Benth.) ETHANOL EXTRACT AS A NATURAL ANTIOXIDANT AND

ANTIBACTERIAL IN TRANSPARENT SOAP

Birgitta Eknis Putri*, Hartati Soetjipto**, Sri Hartini**

*Mahasiswa Program Studi Kimia Fakultas Sains dan Matematika

**Dosen Program Studi Kimia Fakultas Sains dan Matematika

Universitas Kristen Satya Wacana, Salatiga

Jl. Diponegoro No. 52-60, Salatiga 50711, Jawa Tengah – Indonesia

652010006@student.uksw.edu

ABSTRACT

The objectives of this study were to determined the total phenolic content, antioxidant

activity and antibacterial activity of ethanol extract of the sausage fruit as the natural

active ingredients in soap transparent. The sausage fruit extract was obtained by ethanol

maceration method. The total phenolic content was measured by folin ciocalteau

reagent, antioxidant activity was test by using DPPH (1,1-Diphenyl-2-Picryl-hidrazyl)

reagent where as antibacterial activity was performed by using paper discs method

againts gram positive bacteria [Bacillus subtilis (ATCC 6051)] and gram negative

bacteria [Escherichia coli (ATCC 0091IFO)]. The quality of transparent soap which is

obtained in this study was compared to SNI 06-3532-1994 bath soap. The extract was

concentration were used in this study are 0%; 0,5%; 1,0%; 1,5%; 2,0%; 3,0%; and

4,5%. the data were analized by Randomized Completely Block Design (RCBD), 7

treatments and 4 replications. The average total phenolic content, antioxidant

activity and IC50, and Minimum Inhibitor Concentration (MIC) were determined with

the test Honestly Significant Difference (HSD) with significance level 0f 5%. The

results of the study showed that the total phenolic content of the extracts was

6,45±0,59 mg EAG/ g and antioxidant activity with IC50 8,56±0,84 mg/ L. Antibacterial

activity of the sausage fruit extract was more effective to gram positive than gram

negative bacteria. The optimal concentration of fruit extracts in transparent soap making

process was 2,0%. The transparent soap obtained fulfilled SNI 06-3532-1994, except

for the value of moisture content.

Keywords: Kigelia africana, polyphenols, antioxidant, antibacterial, transparent soap