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A. D. PFSC (relative to 3 µm beads). B. SSC (relative to 3 µm beads). C. OFSC/PFSC. Supplementary Figure S1. A. Growth rate (day -1 ). Light level (µmol phot m -2 s -1 ). B. Growth rate (day -1 ). [CaCl 2 ] (mM). Supplementary Figure S2. A. B. PFSC (relative to 3 µm beads). - PowerPoint PPT Presentation
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PFSC (relative to 3 µm beads)
SSC (relative to 3 µm beads)
OFSC/PFSC
A.
B.
C.
Supplementary Figure S1
D.
[CaCl2] (mM)
Light level (µmol phot m-2 s-1)
Gro
wth
rat
e (d
ay-1)
Gro
wth
rat
e (d
ay-1)
A.
B.
Supplementary Figure S2
PFSC (relative to 3 µm beads)
PF
SC
(re
lati
ve to
3 µ
m b
eads
)S
SC
(re
lati
ve to
3 µ
m b
eads
)O
FS
C/P
FS
C
SSC (relative to 3 µm beads)
OFSC/PFSC
Light level (µmol phot m-2 s-1)
Light level (µmol phot m-2 s-1)
Light level (µmol phot m-2 s-1)
Supplementary Figure S3
A. B.
C. D.
E. F. G.
PFSC (relative to 3 µm beads)
SSC (relative to 3 µm beads)
OFSC/PFSC
PF
SC
(re
lati
ve to
3 µ
m b
eads
)S
SC
(re
lati
ve to
3 µ
m b
eads
)O
FS
C/P
FS
C
Light level (µmol phot m-2 s-1)
Light level (µmol phot m-2 s-1)
Light level (µmol phot m-2 s-1)
A. B.
C. D.
E. F. G.
Supplementary Figure S4
A.
C.
E.
B.
D.
F.
OFSC/PFSC OFSC/PFSC
OFSC/PFSC OFSC/PFSC
OFSC/PFSC OFSC/PFSC
Supplementary Figure S5
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
100 101 102 103 104
FSCV
10-2
10-1
100
101
102
Supplementary Figure S6
A.
C.
E.
B.
D.
F.
Empty coccospheres
Detached coccoliths
Debris, bacteria, precipitates
Debris, bacteria, precipitates
Likely empty organic casings of released from empty coccospheres
PFSC PFSC
PFSC PFSC
PFSC PFSC
OF
SC
OF
SC
OF
SC
OF
SC
OF
SC
OF
SC
A.
B.
C.
D.
E.
F.
OFSC/PFSC OFSC/PFSC
SSC (relative to 3 µm beads) SSC (relative to 3 µm beads)
PFSC (relative to 3 µm beads) PFSC (relative to 3 µm beads)
Supplementary Figure S7
B.
C.
Supplementary Figure S8
A.Distal shield Proximal shield
Probable most preferred orientations: Coccolith axis perpendicular to flow stream
Probable less preferred orientations: Axis parallel to flow stream
C.
Laser
Sheath Fluid
Sample Core
Laser
Sheath Fluid
Sample Core
Sample Core
Sheath Fluid
Laser
A. B.
Supplementary Figure S9
SSC (relative to 3 µm beads)
SSC (relative to 3 µm beads)
SSC (relative to 3 µm beads)
SSC (relative to 3 µm beads)
OF
SC
/PF
SC
OF
SC
/PF
SC
OF
SC
/PF
SC
OF
SC
/PF
SC
Detached coccoliths, E. huxleyi grown in F/4, 12.5 µmol phot m2 s-1:
Intact coccoliths
Detached coccoliths, E. huxleyi grown in
artificial seawater,10.5mM
[CaCl2] :Intact heavily
calcified coccoliths
Detached coccoliths, E. huxleyi grown in
artificial seawater, 0.6 mM [CaCl2]:
Intact, but lightly calcified coccoliths
Detached coccoliths, E. huxleyi grown in
artificial seawater, 0.3 mM [CaCl2] :
Poorly formed coccoliths
Supplementary Figure S10
