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TWF1 Polyclonal ANTIBODYCatalog Number:11732-1-AP Featured Product 1 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:11732-1-AP
Size:150UL , Concentration: 133 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG2332
GenBank Accession Number:BC022344
GeneID (NCBI):5756
Full Name:twinfilin, actin-binding protein,homolog 1 (Drosophila)
Calculated MW:368 aa, 42 kDa
Observed MW:42 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:1000-1:6000 IHC 1:50-1:500
Applications Tested Applications:IHC, WB, ELISA
Cited Applications:IF, WB
Species Specificity:human, mouse, rat
Cited Species:human
Note-IHC: suggested antigen retrievalwith TE buffer pH 9.0; (*) Alternatively,antigen retrieval may be performedwith citrate buffer pH 6.0
Positive Controls:
WB : C2C12 cell,
IHC : human lung cancer tissue, human breast cancertissue
Background Information
Notable Publications Author Pubmed ID Journal Application
Lu Li 28864421 Biochem Biophys Res Commun WB,IF
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 11732-1-AP (TWF1antibody) at dilution of 1:3000 incubated at roomtemperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using11732-1-AP (TWF1 antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using11732-1-AP (TWF1 antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).