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European and Mediterranean Plant Protection Organization

Organisation Europenne et Mditerranenne pour la Protection des Plantes

PP 1/4(4)

Efficacy evaluation of fungicides

Uncinula necator

Specific scope Specific approval and amendment

This standard describes the conduct of trials for the efficacyevaluation of fungicides against Uncinula necator, causing

powdery mildew of grapevine.

First approved in 1977-09.evision approved in 19!7-09.

"ligned with revised standard te#t in 199$.evision approved in %001-09.

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1. Experimental conditions

1.1 Test organisms, selection of crop and cultivar

Test organism' Uncinula necator()*+*.

/nly productive grapevine Vitis vinifera (T of

the same susceptible cultivar, rootstoc habit and age,should be used.

1.2 Trial conditions

The trial should be set up in the field. The vineyard

should be homogeneous in cultivar, age, plant width,training system, rootstoc and general cultivation andhealth status. +ultural conditions (e.g. soil type,

fertili2ation should be uniform for all plots of the trialand should conform with local agricultural practice.

3icroclimate conditions should as far as possible behomogeneous, particularly with respect to altitude,

slope and wind e#posure.

The trial should form part of a trial series carried out in

different regions with distinct environmental conditionsand preferably in different years or growing seasons

(see 44/ 5tandard 44 161!1 +onduct and reportingof efficacy evaluation trials.

1.3 Design and lay-out of the trial

Treatments' test product(s, reference product and

untreated control, arranged in a suitable statisticaldesign.

4lot si2e (net' at least 10 vines (or sufficient to provideat least 100 leaves and at least 0 bunches for

assessment, as in 8.% on 8 rows. 5ample si2e may beincreased (e.g. 10 leaves and 100 bunches if the

intensity of the disease is not e#pected to be high.

eplicates' at least .

For further information on trial design, see 44/5tandard 44 161% :esign and analysis of efficacy

evaluation trials.

2. Application of treatments

2.1 Test product(s)

The product(s under investigation should be the

named formulated product(s (see 44/ 5tandard 44161!1 +onduct and reporting of efficacy evaluation

trials.

2.2 Reference product

The reference product should be a product nown to be

satisfactory in practice under the agricultural, planthealth and environmental (including climatic

conditions in the area of intended use. n general, typeof action, time of application and method of application

should be as close as possible to those of the test

product.

2.3 ode of application

"pplications should comply with good standardpractice.

2.3.1 Type of applicationThe type of application (e.g. a spray or a dust should

be as specified for the intended use.

2.3.2 Type of equipment

"pplication(s should be made with e;uipment whichprovides an even distribution of product on the whole

plot or accurate directional application whereappropriate, e;uivalent to good commercial practice.

Factors which may affect efficacy (such as operatingpressure, no22le type should be chosen in relation to

the intended use.

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2.3.3 Time and frequency of application

The number of applications and the date of eachapplication should be as specified for the intended use.

The 1st application is normally made at ha-1 should also be given. :eviations from the

intended dosage should be noted.

2.3.5 Data on other plant protection productsf other plant protection products (or any biocontrolagents have to be used, they should be applied

uniformly to all plots, separately from the test product

and reference product. 4ossible interference with theseshould be ept to a minimum.

3. Mode of assessment, recording andmeasurements

3.1 eteorological and edaphic data

3.1.1 Meteoroloical data/n the days before and after application,meteorological data should be recorded which is liely

to affect the development of the crop and6or pest andthe action of the plant protection product. This

normally includes data on precipitation and

temperature. "ll data should preferably be recorded on

the trial site, but may be obtained from a nearbymeteorological station.

/n the date of application, meteorological data should

be recorded which is liely to affect the ;uality andpersistence of the treatment. This normally includes at

least precipitation (type and amount in mm and

temperature (average, ma#imum, minimum in @+. "nysignificant change in weather should be noted, and in

particular its time relative to the time of application.

Throughout the trial period, e#treme weatherconditions, such as severe or prolonged drought, heavy

rain, late frosts, hail, etc., which are liely to influence

the results, should also be reported. "ll data concerningirrigation should be recorded as appropriate.

3.1.2 !daphic data

*ot re;uired.

3.2 Type, time and fre!uency of assessment

The

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3." #ffectson non-target organisms

3.4.1 !ffects on other pests

"ny observed effects, positive or negative, on the

incidence of other pests should be recorded.

3.4.2 !ffects on other non#taret oranisms

"ny observed effects, positive or negative, on naturally

occurring or introduced pollinators or natural enemiesshould be recorded. "ny observed effect, positive or

negative, on adBacent or succeeding crops should berecorded. "ny environmental effects should also be

recorded, especially effects on wildlife.

3.$ %uantitative and !ualitative recording of yield

*ot re;uired. The grapes harvested in the various plotsmay be weighed but e#trapolation of the data is only

valid if the vineyard is homogeneous.

4. Results

The results should be reported in a systematic form andthe report should include an analysis and evaluation.

/riginal (raw data should be available. 5tatistical

analysis should normally be used, by appropriate

methods which should be indicated. f statisticalanalysis is not used, this should be Bustified. 5ee 44/

5tandard 44 161% :esign and analysis of efficacyevaluation trials.

Appendix

To assess percentage of leaf surface and bunch areaaffected, a scale such as the following may be used and

should be described'

1 C no diseaseA

% C D?A8 C -10?A

C 10-%?A

C %-0?A

$ C 0-7?A

7 C E7?.

(from 44/ 5tandard 44 1681$lasmopara viticola

1 C no diseaseA

% C 1- ?A

8 C -% ?A C %-0?A

C E0?.

(from 44/ 5tandard 44 1617 %otryotinia fuc&eliana

on grapevine, bunch area affected

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