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[PPT]Liquid Chromatography Fundamentals - Theory · Web viewLiquid Chromatography Fundamentals - Theory Keywords HPLC, LC, HPLC theory, HPLC fundamentals, teaching HPLC, learning

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Liquid Chromatography Fundamentals - Theory

Building

Better Science

Agilent and You

:

June 6, 2017 5991-8064JAJP

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June 6, 2017 5991-8064JAJP

Agilent Technologies, Inc. 2016

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June 6, 2017 5991-8064JAJP

Agilent Technologies, Inc. 2016

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June 6, 2017 5991-8064JAJP

Agilent Technologies, Inc. 2016

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CE

CE

CE

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()

Arne Tiselius (19021971) 1937 Tiselius

Tiselius (1948 )

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CE

(CE)

:

/

()

()

DNARNA

(-DNA )

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CE

(CZE) (MEKC) (CEC) () (CGE) (CIEF) pH (CITP)

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CE

(CZE)

CZE CE CZE ()

(CEC)

CEC EOF

(MEKC)

MEKC ()

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CE

(CGE)

CGE () DNA SDS

(CITP)

CITP 2

(CIEF)

CIEF (pI)

pH

pH

pH

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CE

CZE (1) (n)2 (23)

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CE

UV

/

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CE ( 150 nL)

:

()

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CEP CEP EOF DNA PVA PVA /EOF SIL-DB SIL-WAXSIL-FC SIL EOF cIEF PCR CE

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UV-Vis (190600 nm)

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UV-VIS

(Lmol-1cm-1)

b (cm)

c

(b) CE ( Z )

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:

(DAD)

:

(MSD)

(LIF)

(CCD)

1024

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17

More details in Fundamentals of High-Performance Liquid Chromatography - Hardware

()

pH EOF

5 10 15 A) 5 B)

[]

[]

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pH 9.5 10 M 20 M E23G E217G (m/z 447.2024)E33G E316G (m/z 463.1974)

: Comprehensive Profiling of Free and Conjugated Estrogens by Capillary Electrophoresis-Time of Flight-Mass

Spectrometry (-)

17- TOF/MS ()

vs. [m/z]

= 0.9 ppm

()

(x103)

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pI (%)SDRSD%SDRSD%A6.5460.0070.1054.290.4710.93B6.4570.0040.05623.220.582.50C6.3650.0030.04232.940.692.08D6.2900.0020.03625.661.485.77E6.2320.0020.02913.891.7612.67

mIgG1 5 AE

: Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing on the Agilent 7100 Capillary Electrophoresis System (Agilent 7100 )

Agilent 7100 CE cIEF

4 CE 4 6 (n=24)

lgG

()

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y = a bxR2LOD (ng/mL)LOQ (ng/mL)SPMy = 0.0056 0.1177x0.9991.96.3SPDy = 0.0144 0.1927x0.9992.68.6PUTy = 0.0112 0.0826x0.9992.58.3HISy = 0.0330 0.2254x0.9991.13.6CADy = 0.164 0.1503x0.9991.65.3

10 g/mL CE-MS/MS

(SPM) (SPD) (PUT)

(HIS) (CAD) (AGM)5 g/mL 1,7- (DIA) () (PHE) (TYR) (TRY) (URO) (SER)

: Analyze Biogenic Amines in Salmon and Shrimp by Capillary Electrophoresis-Tandem MS (- MS )

()

10,000

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www.agilent.com/chem/jp

[email protected]

()Agilent CECE/MS 5991-1511JAJPAgilent 7100 Capillary ElectrophoresisG7100-9000Comprehensive Profiling of Free and Conjugated Estrogens by Capillary Electrophoresis-Time of Flight-Mass Spectrometry5990-9669ENMonoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing on the Agilent 7100 Capillary Electrophoresis System5991-1141ENAnalyze Biogenic Amines in Salmon and Shrimp by Capillary Electrophoresis-Tandem MS5991-6255ENWeb CE/MSWebCHROMacademy

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THANK YOU

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bcCE CECCEP CGECIEFCZECITP EOF e (Lmol-1cm-1)

MEKC MSnL PVATOF ()

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c

b

A

=

e