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HCG (Human Chorionic Gonadotropin)
Dwi AmaliaYuni Sulistiani
Anisa RahmadiniDenny Agung NugrohoD3 ANALIS KESEHATAN
STIKES JENDERAL ACHMAD YANI CIMAHI
TEORITICAL BACKGROUND
HCG (Human Chorionic Gonadotropin) is a hormone produced by placenta tissue in
early pregnancy. This hormone will be released through the urine.
PURPOSE
To detect the presence of human chronic gonadotropin in urine and also detects the presence of pregnancy
The direct pregnancy assay is based upon the agglutination reaction between latex particles coated with anti-hCG antibodies and hCG present in the test specimen.
PRINCIPLE
Tools and Material
Used to protecting the body
Lab Coat Mask
Used to protect nose and mouth
Gloves Slide test
Used to protect hand from infection subtance
Used to interpretation the result
microtube pippette
It can used to pippette and stirring
Used to Place the sample
Lateks reagent Disinfectant
Used to detect antigen in sampel
Used to sterilization pippette & stirring
Control (-)
Used to standard control (-) for sample
Control (+)
Used to standard control (+) for sample
Procedure • Bring the reagent and spesimen to room temperature before use• Place one drop of the negative pregnancy control onto a circle of the
agglutination slide• Using the pippet-stirrers provided, place one drop of the urine specimen
onto the remaining circle of the agglutination slide• Shake and re-suspend the pregnancy latex reagent. Add one drop to each
of the test circles of the agglutination slide• Stir with individual pippete-stirrer and spread mixture over entire area of
the test circle• Gently rock the agglutination test slide for two minutes and observe the
test circles for agglutination. Interpret results at two minutes. Extended incubation may result in evaporation and erroneous result
Interpretation of results
• Positive result : agglutination occurs within two minutes
• Negative result : agglutination does not occur within two minutes
RESULT
• In urine Mrs. .... Negative pregnancy because there’s not occur agglutination. This mean Mrs. ... Is not pregnancy and of course there is in urine not contain antigen anti-HCG.
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