cng cng ngh protein enzyme
Cu hi trc nghim Cu1: Th no l km hm cnh tranh? A. Cht km hm I gn
vi trung tm hot ng ca enzyme E lm chm phn ng to sn phm P . B. Cht
km hm I gn vi phc hp c cht enzyme ES lm chm phn ng to sn phm P. (k
cnh tranh) C. Cht km hm I gn c vi c enzyme t do v phc hp ES lm chm
phn ng to sn phm. (hn hp) D. Tt c u sai. Cu 2: Trong cc nhm cht
sau, nhm cht no cung cp ngun Carbon cho VSV A. Glucid, m, cao ng.
B. Glucid, bnh du u phng, ru. C. M, bnh du u phng, bt tng. D. M,
bnh du u phng, gelatin. Cu3: Sc k trao i ion l phng php sc k da
trn: A. S hp ph chn lc cc cu t ring bit ca hn hp cht phn tch. B. S
sai khc gia cc h s phn b ca cc cu t ring bit ca hn hp cht phn tch.
C. Qu trnh trao i ion gia cc ion linh ng ca cht hp thu v cht in ly.
D. S khc nhau v tan ca cc kt ta to thnh do cc cu t ca hn hp v thuc
th. Cu4: Phng php no s dng sy bng chn khng thng hoa bo qun ging? A.
Phng php cy chuyn. B. Phng php lm lnh. C. Phng php ng kh. D. Phng
php lm lnh trong nitro lng. Cu5: Enzyme cng tinh khit khi: A. Hot
tnh ring E cng cao. B. Hot tnh ring E cang thp. C. Hot tnh tng cng
cao. D. Hot tnh tng cng thp. Cu 6: xc nh trnh t sp xp aa trong mt
on peptid, phng php no c s dng xc nh u Carbon tn cng? A. Sanger.
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cng cng ngh protein enzyme
B. Dansyl. C. Carboxypeptidase. D. Edman. Cu 7: Trong iu ha sinh
tng hp, vng promoter c tc dng nh th no? A. B. C. D. A. B.C.
M ha protein c bit, c ch sao m. Trnh t DNA RNA_polymerase gn vo
bt u qu trnh phin m. M ha phn t protein, enzyme c tng hp. iu khin
qu trnh sao m ca gene cu trc. PP Sanger PP Dansyl PP
carboxypeptidase PP gim cp Edman I: Cn bng. II: Ra gii. III: Np mu.
IV: Ti to ct. A. I, II, III, IV. B. I, III, II, IV. C. III, I, II,
IV. D. III, IV, I, II.
Cu 8: Phng php xc nh u C_tn cng?
D.
Cu 9: Trnh t no di y th hin ng cc bc ca pp sc k trao i ion?
Cu 10: Cc acid amin c chia lm my nhm?(lit k c th tn cc nhm) A.
B. C. D. dng 1.Nguyn tc in di mt chiu gm my bc: A. 2 bc. B. 3 bc.
C. 4 bc. Page 2 3. 4. 5. 6.
Cu 11: bo qun v bo tn c c tnh di truyn trong sut thi gian bo qun
v s
cng cng ngh protein enzyme
D. 5 bc. 2.Quy trnh thu nhn gm nhng nguyn tc no? 1. Xc nh mc tiu
thu nhn. 2. Xc nh tnh cht ca protein mc tiu. 3. S lng cc bc tinh
sch thp nht. 4. K thut tinh sch cho mi bc. A. 1 v 2. B. 1, 2 v 3.
C. 3 v 4. D. Tt c u ng. 3.nh hng ca nng enzyme n tc phn ng nh th no
? A. B. C. D. E. A. B. C. D. A. B. C. D. A. B. C. D. [S]
>>[E]: vn tc phn ng ph thuc vo [E]. [S] >>[E]: vn tc
phn ng ph thuc vo [S]. [S] nap mu -> ra giai -> tai tao ct.
C. Ra giai -> nap mu -> tai tao ct -> ra giai. D. Cn bng
-> nap mu -> tai tao ct -> ra giai. Cu 16 :in di im ng in
dung : A. Xac inh tn protein va phn tach protein. B. Xac inh pI cua
1 protein. C. Phn tach protein da trn im ng in. D. B,C u ung. E. Tt
ca u ung. Cu 17 : tinh sach cua protein, enzyme khi s dung trong dc
phm phai at bao nhiu: A. 85-95% B. 95-99% C. >99% D.
