Sebaceous Lipidic Techniques

8/12/2019 Sebaceous Lipidic Techniques

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Sebaceous / Lipidic Techniques

GENTIAN VIOLET FORMULA:FORMULA A.

1 ml Gentian Violet solution1000 ml distilled water.

FORMULA B.

1 g Gentian Violet crystals

1000 ml distilled water.

PROCEDURE OF APPLICATION

1. Pass te item troug a !ray

containing te reagent solution

"or 1 to # minutes.

#. $old ta% water rinse & '0 seconds.

'. View (isually) or wit a

"orensic ligt source *etween

+0+ nm & +,0 nm wit red goggles.

-. $LAR/G OLU!/O2

10 ml 3ydrocloric acid in

40 ml o" ta% water. 5106 soln.7

Process Summary:

This is a dye staining process using a water-based working solution. The evidence isrepeatedly stained and rinsed until optimum development occurs. This reagent may beapplied to surfaces that are contaminated with oils and grease.

Accepted De!at!o"s:

The working solution may be re-used. The working solution may also be applied by

brushing. The developed detail may be viewed under a forensic light source at 505 nm to570 nm using red viewing goggles.

IODINE FUMING FORMULA:

e(eral commercial models o"/odine Fuming 8its and

cam*ers are a(aila*le. Read

manu"acturer9s instructions.

Pac8 a*out 1:# teas%oon o"

iodine crystals into te

"uming 8it:cam*er.

Follow te


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manu"acturer9s instructions.

PROCEDURE OF APPLICATIONPre%are te %otogra%ic

set&u% *y %re&setting

camera ligting) a%erature

and sutter s%eed.

Low tem%erature eat is

re;uired to su*limate te

iodine crystals into "umes.

Pass te "umes o(er te

sur"ace to *e e.,4 g odium %os%ate di*asic e%taydrate


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dissol(ed in 1 L distilled water

a8e *ot solutions until dissol(ed) ten com*ine.

Add distilled water to increase te com*ined

*u""er solutions (olume to - L.

5 Alternati(e 7

$ARBOA! %, BUFFR OLU!/O

#?.+ g odium car*onate

dissol(ed in # L distilled water

Add 5care"ully7 1>.' ml

concentrated itric acid.

a8ing constantly.

Add distilled water to increase

te *u""er solution (olume to #.+ L.

PROCEDURE OF APPLICATION

1. /mmerse te item in stain solution

and soa8 com%letely. /t is o%tional to

agitate te solution on a sa8er %lat"orm.

& Ridge detail sould *egin to de(elo% in + min.

& @ea8 "inger%rints 5%oor li%idic content7 may

re;uire ?0 to 40 minutes o" de(elo%ment time.

#. Remo(e = drain item & immerse in

one o" te *u""er solutions.

'. Remo(e item "rom *u""er solution &

rinse in distilled water.

-. ry item at room tem%erature &

or eat in an o(en at +0 degrees $.

Process Summary:

A lipid-specific dye stain technique for use on porous items which have become wet.(esearch has shown that )il (ed ) can be superior to *hysical +eveloper to develop

latent fingerprints on thermal paper and standard white paper. %ewer immersion steps and

less need to avoid chloride contamination are benefits using the )il (ed ) reagent.Accepted De!at!o"s:The ,arbonate p7 buffer solution may be used instead of the *hosphate p7 buffer

solution.

)il (ed ) may be used as a precursor to *hysical developer to locate areas of likely

friction ridge detail on brown kraft paper. *hysical developer treatment follows )il (ed

) in order to improve the resolution of the friction ridge detail.


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P#$SICAL DEVEOPER FORMULA:1.

Maleic acid %re&was2

+0 grams Maleic acid %owder

dissol(ed in # liters

o" distilled water.

#.

$ommercial @or8ing olution2

1 %art solution A

5+ml. :10ml. :1+ml.7

!o 1> %arts solution B

540ml.:1>0ml.:#,0ml.7

PROCEDURE OF APPLICATIONA.

Pre&was 510 minutes7

B.

@or8ing solution

5#0 minutes7

$.

Rinse 5+ minutes7

.

Rinse 5+ minutes7

Process Summary:

This is a multi-solution multi-step process that can be used as a follow-up to ninhydrin

cases. This is the technique of choice for paper currency items and porous items that may

have been wet.

Accepted De!at!o"s:,ommercial or #aboratory-prepared mi&tures are used. *hysical +eveloper also develops

indentations in paper surfaces therefore %/TT impressions may be enhanced.

SILVER NITRATE FORMULA:FORMULA A

5 1.0 6 solution 7

1 g il(er nitrate

100 ml distilled water

FORMULA B

5'.0 6 solution7

'0 g il(er nitrate


FORMULA $

5Alcool&*ased solution7


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'0 g il(er nitrate


1000 ml tanol

PROCEDURE OF APPLICATION1. a7 !ray immersion o" item

"or + seconds.

*7 Reagent solution *rused

onto item until coated.

#. Air dry "or #0 minutes.

'. unligt or U.V. ligt

e

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Sebaceous Lipidic Techniques