PREPARED BY KYLE SUNG

AUTOMATED NUCLEIC ACID ISOLATION SYSTEMS

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PRODUCT INTRODUCTION

Automated Nucleic Acid Isolation Systems

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PRODUCT DESIGN

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Design

Industrial Design

Electrical

Thermal Control

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Feedback Control

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PCBA

Mechanical Design

INTENDED USE OF SOLMAG®

Magnetic Bead Separation: Bench-top automated system for quick purification of nucleic acids from bio-

specimens using magnetic bead separation technology.

Liquid Handling System: Liquid handling automation, using pre-filled reagent cartridges

For research and in vitro diagnosis use

FUNCTION PRINCIPLE

Advanced Liquid Handling Magnetic Bead Separation

Sample Material Lysis Step Binding Step

Magnetic Separation Washing Step Elution Step; Purified Nucleic Acid

CORE TECHNOLOGIES

The Solis-Charger® Technology

The Solis-ChargerR Technology is a novel magnetic bead-based technology that provides a switchable surface charge dependent on the pH and salt concentration of the surrounding buffer to facilitate nucleic acid purification. In low pH conditions, the magnetic beads have a positive charge that binds the negatively charged nucleic acid backbone (see figure1 below). Proteins and other contaminants are not bound and are simply washed away in an aqueous wash buffer. To elute nucleic acids, the charge on the surface of the bead is neutralized by raising the pH to 8.0 using a low salt elution buffer (see figure below). Purified nucleic acid elutes instantly into this elution buffer, and is ready for use in downstream applications.

CORE TECHNOLOGIES

The 2D CatcherMag® Technology for Sample Preparation

The 2D CatcherMag® technology is a novel magnetic bead-based modification technology. The 2D CatcherMag® is modified and designed for different sample types to optimized collection/extraction result. With the nano-scale modification on the 2D CatcherMag®, we can provide the users a better solution while collecting the samples for reliable polymerase chain reaction results and accurate data analysis.

The 2D CatcherMag® adhering to pathogen in the collection tube

SOLMAG ® 12

Automated Nucleic Acid Purification Systems

SolMag®12: Compact Bench-top Instrument Process 1-12 samples simultaneously 7 inches touch screen Light: Total weight less than 42 Kg Fast: Up to 12 results in 35 minutes Precise liquid volume and temperature control Extremely easy to operate and maintain

SOLMAG ® 12

Front View of Solmag®12

Transparent Maintenance WindowThe Transparent Window is designed to let the user have a clean and sharp look inside the system during operation.

Seven Inches Full-ColoredThe full-colored touch-screen will simplified and guide you through the setup procedure.

Reagent & Plastic Disposable DrawerOpen the drawer to load the ready-to-use prefilled reagent cartridges and other plastic consumables required for performing a nucleic acid purification reaction.Rubber Hand Holders Hand Holders are designed to let users move the instrument with comfort.

SOLMAG ® 12

Inside View of Solmag®12

Six-linked PrefilledReagent Cartridges

Tip Set

Regular Filtered Tip

Small Filtered Tip

Sample TubeTransferrable Sample Rack

Elution Tube

SOLMAG ® 12

Back View of Solmag®12

USB Connection Port

RS232 Connection Port

Power Outlet

Power Switch

SOLMAG ®12 SPECIFICATIONS

Light as feather: SolMag®12 weights only

42kgw, which is 50% lighter in average than other similar throughputs liquid handling extractor in the market.

MagNaPure Compact: 60Kg

Qiagen EZ1 Advanced XL: 48Kg

MagCore HF16: 70Kg

SolMag®12Part No. SVP0101

Dimensions (W x D x H) 637mm x 462mm x 540mm

Weight 42kgw

Interface 7” LCD touch-screen

Processing time 20 – 45mins

Throughput 1-12 Samples per run

Temperature Uniformity ±0.5℃

Liquid Extraction Volume Error Less than 5％

Sample Volume 50ul – 3000ul

Elution Volume 30ul – 200ul

Power Input power :90 ~ 132V&180 ~ 264Vby switch50/60HzOut power :5V60A

Certification CE、IVD、FDA

UNIQUE SPECIFICATIONS

7” Touch Screen: Optimized built-in protocol covers six major applications such as clinical

diagnostic, forensic science, food safety and CFC.

Built-in Template: Up to three preferable

templates can be set up by the users for performing the same routine protocol daily, which can also reduce the risk from human operational error.

UV LAMP DECONTAMINATION

• With a built-in UV-lamp, the UV decontamination guarantee maximum safety for both samples and environment

ABOUT INSTRUMENT

SLEEP MODE

SOFTWARE UPDATE

DEVICES TESTS

UV LAMP

SOLMAG ® 24

Automated Nucleic Acid Isolation Systems

SolMag® 24:The SolMag®24 provides all the advantages of the SolMag®12, and increases the throughput to 24 samples per run. This new workstation has been developed to combine the convenience and reliability of the SolMag® Series with the need for low-to-medium throughput.

SOLMAG ISOLATION KITS

Ready-to-use pre-filled, Reagent Cartridge: The SolMag® Nucleic Acid Isolation kits contain all reagents and

disposables needed for 48 nucleic acid isolation reactions.

SolMag® NA Isolation Kits

1. Blood/Tissue Nucleic Acid Isolation Kit

2. Virus Nucleic Acid Isolation Kit

3. Pathogen Nucleic Acid Isolation Kit

4. Food Safety Nucleic Acid Isolation Kit

5. Forensic Science DNA Isolation Kit

6. Circulation Nucleic Acid Isolation Kit

7. Virus Nucleic Acid Isolation Kit - Large Volume

WORK STATION

Mid to high throughputs• >= 24 preps/run

Programmable robot arm controlled by separated computerBig instrument dimension / heavy weightComplicated setup procedures /require additional training before operationHigher instrument and consumable cost

• > US$ 80KEx:

• Qiasymphony(Qiagne)• Cobus (Roche)

STANDALONE

Small to mid throughputs

• < 24 preps/run Built-in micro-processor and protocols, no PC required

Small Footprint / compact

Easy setup and operation steps

Lower instrument and consumable cost

• <US$10KEx:

• SolMag (Solison), EZ1 Advanced (Qiagen), MagNa Pure Compact (Roche), iPrep (Invitrogen) … etc

COMPARISON WITH WORK STATION

Advantage• Fully walk away automation:

minimum procedures of set up, which require three simple steps.

• Enable to handle a wide range of volumes on starting materials and elutes

• Better extraction qualityDisadvantage

• Bigger instrument size and heavier weight

• Higher instrument costEx:

• SolMag (Solison), EZ1 Advanced (Qiagen), MagNa Pure Compact (Roche), iPrep (Invitrogen) … etc

MAGNETIC ROD

Advantage• Lower kit price

Disadvantage• Easy to have magnetic bead

residues in eluted products• Limited or fixed sample / elute

volume handling choice• Semi-Automation: tedious

manual set up procedure required, kills time and cause humor errors.

Ex:• Maxwell (Promega)• KingFisher (Thermo Fisher)

LIQUID HANDALING

COMPARISON WITH MAGNETIC ROD SYSTEM

THANK YOU FOR YOUR ATTENTION!!

Solison Biotechnology Corp.Web: www.solisonbio.comAdd: No. 3-2, Chien-kuo road, TEPZ Tantzu, 427 Taichung, Taiwan R.O.CTel: +886 4 2531 1334 Fax: +886 2 2531 1343Mail: info@solisonbio.com