Specific Mutation Induced by Triplex-Forming Oligouncleotides in Mice

Karen M. Vasquez, Latha Narayanan, Peter M. Glazer

20 October 2000 Science

Date: 2001 4/25

Speaker: 劉育男

Introduction:

~Aptamer

~SELEX

Triplex-Forming Oligonucleotide

(TFOs)

TFO 發展面臨的問題

1.Cell uptake

2.Stability of TFO

3.Specific targeting site or potential site

4.New DNA damage agent

5.Triplex-binding code

Conclusion:

These studies established that small DNA molecule can be administered by i.p. or intravenous injection and gain access to tissue and to cell nuclei

Chromosomal DNA throughout the somatic tissues of an animal can be targeted by nucleic acid aptamers

Aptamers 的應用

~ 取代 antibodies

作為更專一性的結合 ligand

Medical usage, Lab usage

~ 調控 Cell bioactivities

Drug design

The End

Thank you