Statusseminar ChiptechnologienTechnologien und Anwendungen

03. - 04. Februar 2005

Vorbereitungsgremium

Dr. U. Brüggemeier, Bayer AG, WuppertalProf. Dr. T. Gress, Universitätsklinikum UlmDr. J. Hoheisel, DKFZ, Heidelberg (Koordinator)Dr. T. Joos, NMI, ReutlingenDr. R. Lenke, DECHEMA e.V., Frankfurt am MainProf. Dr. P. Lichter, DKFZ, Heidelberg

Begleitende Ausstellung (Stand: 20.01.2005)

Folgende Firmen werden sich als Aussteller beteiligen:

• Agilent Technologies Sales & Services GmbH & Co. KG, Waldbronn• Biozym Scientific GmbH, Hessisch Oldendorf• Eppendorf AG, Hamburg• GeSiM - Gesellschaft für Silizium-Mikrosysteme mbH, Großerkmannsdorf• Greiner Bio-One GmbH, Frickenhausen• iito Bremen Deutschland, Bremen• Implen GmbH, München• MicroDiscovery GmbH, Berlin• Miltenyi Biotec GmbH, Bergisch Gladbach• MMI AG, Glattbrugg/CH• Operon Biotechnologies GmbH, Köln• PerkinElmer LAS Deutschland GmbH, Rodgau• PROGEN Biotechnik GmbH, Heidelberg• RANDOX Laboratories GmbH, Krefeld• Röche Diagnostics GmbH, Mannheim• RZPD Deutsches Ressourcenzentrum für Genomforschung, Berlin• Schleicher & Schuell BioScience GmbH, Dassel• SCHOTT Nexterion AG, Mainz• Scienion AG, Berlin• Tecan Deutschland GmbH, Crailsheim• Universität Freiburg

Tagungsbüro

DECHEMA e.V.Frau Xueqing WuTheodor-Heuss-Allee 2560486 Frankfurt am MainTel.: 069/7564-152, Fax: 069/7564-304Email: wu@dechema.dehttp://www.dechema.de/chips on

TIB/UB Hannover 89126 230 773 _

Vortragsprogramm / Lecture Programme

Page10:30 Welcome

G. Kreysa, DECHEMA e.V., Frankfurt am Main

Chip TechnologiesChair: J. Hoheisel, DKFZ, Heidelberg

10:40 Qualitätssicherung und Biochips, ein Paradoxon? DefinierteOberflächenarchitekturen für DNA, Protein und ZellchipD. Freidank, T. Neumann, G. Dahme, H. Klapproth, M. Reimann,R. Toomey, A. Wörz, M. Biesalski, O. Prucker, J. Rühe,University of Freiburg

11:00 A novel process for manufacturing of high densitymulti-purpose chemical micro-arraysA. Dikmans. U. Beutling, R. Frank, GBF, Braunschweig

11:20 Plenary LectureNew tools to probe molecular composition and architectureU. Landeqren. M. Nilsson, J. Baner, T. Conze, F. Dahl,O. Ericsson, S. Fredriksson, M. Gullberg, S. Gustafsdottir,J. Göransson, M. Howell, J. Jarvius, M. Jarvius, H. Johansson,M. Kamali, C. Larsson, O. Söderberg, E. Schallmeiner,J. Stenberg, Rudbeck Laboratory, Uppsala/S

12:05 Coffee Break and Poster Discussion

12:20 A novel peptide-substrate-microarray for protein tyrosine 6phosphatases prepared by means of Staudinger ligationM. Köhn12. R. Wacker3, H. Schröder3, R. Breinbauer1 *C M . Niemeyer2, H. Waldmann1'2

1 MPI for Molecular Physiology, Dortmund2 University of Dortmund3 chimera biotec GmbH, Dortmund

12:40 Generalized Venn diagrams: a new method of visualizing 8complex genetic set relationsH.A. Kestler, A. Müller, M. Buchholz, T.M. Gress,University of Ulm

13:00 Lunch

Vortragsprogramm / Lecture Programme

Thursday, February 3, 2005

Chair: U.Chip Technologies*Brüggemeier, Bayer AG, Wuppertal

Page14:30 Protein array technology: challenges in chip-based 9

diagnostic SystemsH. Homauer, U. Klause, H.-J. Müller, B. Risse,Röche Diagnostics GmbH, Penzberg

14:50 Analysis of signaling networks with phosphorylation 10specific antibody array tubes®S. Wölfl. University of Heidelberg; A. Ahmad, S. Knoth, L. Pusch,L. Wang, University of Jena; R. Ehricht, Clondiag ChipTechnologies GmbH, Jena

15:10 The ArrayTip platform: a new microarray concept combining 11Flow-Thru and waveguide technologyS.K. Dertinqer, M. Klühr, CA. Eisner, A. Sauermann, K. Rüffer,P.M. Nicklaus, Infineon Technologies AG, München

15:30 A DNA-derivative for improved studies on ZIP-coded 13universal arraysN.C. Hauser1, R. Martinez2, S. Rupp1, J. Hoheisel2, S. Matysiak3

1 Fraunhofer IGB, Stuttgart2 DKFZ, Heidelberg3 Applied Biosystems, Foster City, CA/USA

15:50 Rapid kinetic analysis of hybridoma samples and multi- 15protein drug compound screening using Biacore's SPRarray technologyD. Myszka, University of Utah, Salt Lake City, UT/USA;P. Säfsten, H. Widegren, H. Nordström, M. Hämäläinen,Biacore AB, Uppsala/S

16:10 Coffee Break and Poster Discussion

Vortragsprogramm / Lecture Programme

Toxicology & InfectiologyChair: T. Joos, NMI, Reutlingen

16:40 Toxicogenomics: computational strategies for prediction oflong-term toxicity of novel drugsJ. König, Genedata AG, Basel/CH

17:00 Protein biochips for binder validationA. Lüking, Ruhr-University Bochum; V. Gruß, P. Weingarten,C. Hüls, S. Müllner. Protagen AG, Dortmund

17:20 High-speed microarrays for the detection of antibiotics inmilkB.G. Knecht, M. Eiberle, R. Niessner, Technical University ofMunich; A. Strasser, R. Dietrich, E. Märtlbauer,Ludwig Maximilian University of Munich;M.G. Weller, Technical University of Munich

17:40 Multiparameter-Diagnosesystem für humane AntikörperK. Neumeier, K. Bock, L. Meixner, E. Pringsheim,W. Scheithauer, M. Wackerle, F. Wenninger, Fraunhofer Institutefor Reliability and Microintegration, Munich; G. Backert,D. Reißenweber, V. Straßner, Scherer & Trier GmbH & Co. KG,Michelau; O. Wolfbeis, Chromeon GmbH, Regensburg; B. Halm,M. Motz, K.-l. Pfrepper, MIKROGEN GmbH, Martinsried;P. Lindner, H. Wolf, University of Regensburg

18:00 Poster Party

19:30 End of the First Day

Page

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Vortragsprogramm / Lecture Programme

Biomedical ApplicationsChair: T. Gress, Universitätsklinikum Ulm

Page9:30 High throughput profiling of cancer serum biomarkers 20

C. Zarozinski, R. Negm, D. Pawlak, J. Beator, M. Harvey,B. Parker, Schleicher & Schuell BioScience GmbH, Dassel

9:50 Patient's sample profiling using miniaturised and 21parallelised sandwich immunoassaysS. Wittemann1, E.M. Schneider2, A. Döttinger1, M.F. Templin1,M. Schmolz3, T.O. Joos1

1 NMI Natural and Medical Sciences Institute at the University ofTübingen

2 University Clinic Ulm3 EDI (Experimental & Diagnostic Immunology) GmbH, Reutlingen

10:10

10:55

11:25

Plenarv LectureThe diagnostic proteome: advances in quantitation ofcandidate markers for clinical validationL. Anderson, Plasma Proteome Institute, Washington, DC/USA

Coffee Break and Poster Discussion

Chair: M. Frohme, DKFZ, Heidelberg

Plenarv LectureMolecular, cellular and clinical biochip technologies for„canceromics" research and drug developmentO. Kallioniemi, VTT Medical Biotechnology, Turku/FIN

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12:10 Profiling the autoimmune repertoire of Alopecia areata 24patients and development of a disease-associated proteinchip suitable for diagnosisA. Lükinq, Ruhr-University Bochum; O. Huber, C. Wirths, Charite,Berlin; K. Schulte, Ruhr-University Bochum; U. Blume-Peytavi,Charite, Berlin; H.E. Meyer, DJ. Cahill, Ruhr-University Bochumand Protagen AG, Dortmund

12:30 Cancer diagnosis and prognosis by means of studying 25genomic DNA methylation patternsV. Beier, C. Mund, P. Bewerunge, H.-J. Gröne, F. Lyko,J. Hoheisel, DKFZ, Heidelberg

12:50 Lunch

Vortragsprogramm / Lecture Programme

Biomedical ApplicationsChair: M. Hahn, DKFZ, Heidelberg

Page14:20 Analysis of protein complex formation in T cell signalling by 27

peptide microarrays and fluorescence correlationspectroscopy0. Stoevesandt. M. Elbs, K. Köhler, University of Tübingen;A.C. Lellouch, Universite de la Mediterranee, Marseille/F;R. Fischer, T. Andre, R. Brock, University of Tübingen

14:40 Micro-array based screening of patients with mental 28retardation and multiple congenital abnormalities forsubmicroscopic deletions and duplicationsK. Kok, T. Dijkhuizen, B Sikkema-Raddatz, P. van der Vlies,H. Zorgdrager, Y. Swart, A.Y. van derVeen, T. van Essen,K. Gerssen-Schoorl, C.H.C.M. Buys,University Hospital Groningen/NL

15:00 Mouse protein arrays from a TH1 cell cDNA library for 30antibody screening and serum profilingC. Gutiahr1, D. Murphy3, A. Lüking14, A. König1, M. Janitz1,J. O'Brien3, B. Korn5, S. Hörn1, H. Lehrach1, DJ. Cahill2'3'4

1 MPI for Molecular Genetics, Berlin2 PROTAGEN AG, Dortmund3 Royal College of Surgeons in Ireland, Dublin/IRL4 Ruhr-University Bochum5 Resource Center for Genome Research, Heidelberg

15:20 Phosphorylation assays on antibody arrays with proteins of 31antithrombin-3 treated endothelial cells identify anti-angiogenic markers with a potential in tumor therapyC. Maercker, RZPD, Heidelberg; A. Abdollahi, DKFZ, Heidelberg;C. Rutenberg, RZPD, Heidelberg; O. Andersson, G.S. Tchaga,BD Clontech, Palo Alto, CA/USA; B. Korn, RZPD, Heidelberg;P.E. Huber, DKFZ, Heidelberg

15:40 Closing RemarksChair: J. Hoheisel, DKFZ, Heidelberg

15:50 End of the Seminar

Posterprogramm

Nr. Seite

1 RNA signatures in rheumatoid arthritis 33D. Koczan, University of Rostock; S. Drynda, University of Magdeburg,Vogelsang; P. Lorenz, M.O. Glocker, University of Rostock; R. Guthke,Hans Knoell Institute for Natural Products Research, Jena; P. Ruschpier,P. Stiehl, University of Leipzig; J. Kekow, Hans Knoell Institute for NaturalProducts Research, Jena; H.-J. Thiesen, University of Rostock

2 Establishment of Illumina custom arrays for hepatotoxicity screening 35N. Zidek. P.-J. Kramer, P.G. Hewitt, Merck KGaA, Darmstadt

3 High-density microarry technology: efficient synergy of experimental 36design, array production and data analysisB. Spielbauer, J. Landgrebe, M.K. Jaiswal, B.U. Keller, Universität Göttingen

4 From genome wide screening to content development of diagnostic 37applicationsH. Eickhoff, Scienion AG, Berlin

5 Global analysis of the response to nitrogen limitation in 38Corynebacterium glutamicum using DNA microarrays and2-D PAGEM. Silberbach, M. Schäfer, R. Krämer, A. Burkovski, Universität Köln

6 Comparison of small sample' and Standard RNA amplification 39protocols for hybridization on Affymetrix GeneChipsF. Wagner. J. Maurer, U. Radelof, Deutsches Ressourcenzentrum fürGenomforschung, Berlin

7 Computer-controlled regulation of motor protein in a versatile 40microfluidic System for the fluorescence microscopeF.-U. Gast, S. Howitz, GeSiM mbH, Großerkmannsdorf; S. Diez,MPI of Molecular Cell Biology and Genetics, Dresden

8 Preliminary results of a feasibility study: development of protein 41Chips for the detection of mycotoxins and allergenic proteins infood stuffsM. Janotta. B. Herbinger, University of Applied Sciences Wr. Neustadt/Tulln/A;I. Leitzenberger, R. Krska, University of Natural Resources and Applied LifeSciences, Vienna/A

9 On the way to a prognostic chip for early staged colon carcinomas 42W. Kemmner. C. Astrosini, W. Haensch, P.M. Schlag,Charite Universitätsmedizin Berlin

Posterprogramm

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10 New insights into the functioning of Trypanosoma brucei 43S. Brems. DKFZ, Heidelberg; C. Clayton, University of Heidelberg;J. Hoheisel, DKFZ, Heidelberg

11 Strategies for Signal enhancement of antibody arrays 44U. Sauer, C. Preininger, ARC Seibersdorf research GmbH/A

12 Biochip for detection of endocrine disrupting chemicals (EDCs) 45C. Preininqer, U. Sauer, K. Derwinska, ARC Seibersdorf research GmbH/A

13 New tools for protein microarray technologies 46H. Schröder, chimera biotec GmbH, Dortmund; P. Alhorn,M. Fleger, Universität Dortmund; R. Wacker, chimera biotec GmbH,Dortmund; A. Neyer, CM. Niemeyer, Universität Dortmund

14 Von der Forschung zur Diagnostik - Bioinformatik in der 47Microarray-TechnologieJ. Schuchhardt, F. Kleinjung, D. Beule, A. Malik, MicroDiscovery GmbH, Berlin

15 New platforms and Systems for DNA microarrays: 70mer 48oligonucleotide probes offer excellent sensitivity and specificityB. Henze, B. Saal, D. Drutschmann, K. Wellesen, P. Schüßler,Operon Biotechnologies GmbH, Köln

16 Multiple spotting technique: performing multiplex assays on a 49Single chipP. Angenendt, DKFZ, Heidelberg; D. Cahill, H. Lehrach, J. Glökler,MPI for Molecular Genetics, Berlin

17 Self-assembled protein microarrays generated by DNA-directed 50immobilization (DDI)R. Wacker, H. Schröder, S. Schulz, chimera biotec GmbH, Dortmund;U. Feldkamp, CM. Niemeyer, Universität Dortmund

18 Genotyping highly pathogenic bacteria with oligonucleotide 51microarraysU. Nübel. M. Antwerpen, Robert Koch-Institut, Wernigerode; E. Ehrentreich-Förster, E. Reiß, F. Bier, Fraunhofer IBMT, Potsdam; D. Naumann,Robert Koch-Institut, Berlin; W. Witte, Robert Koch-Institut, Wernigerode

Posterprogramm

Nr. Seite

19 Tumor specific gene expression analysis in renal cell carcinomas 52via gene expression profiling, real-time RT-PCR andimmunohistochemistryJ. Dieqmann. J. Rosenhahn, Friedrich-Schiller-University, Jena; B. Gerstmayer,memorec biotech GmbH, Köln; K. Junker, W. Hindermann, F. von Eggeling,Friedrich-Schiller-University, Jena

20 Generation of protein microarrays by means of expressed protein 53ligation and DNA-directed immobilizationM. Lovrinovic, M. Spengler, Universität Dortmund; R. Wacker, H. Schröder,chimera biotec GmbH, Dortmund; CM. Niemeyer, Universität Dortmund

21 Detection of HIV drug resistance mutations by on-chip primer 54extensionM. Schanne. DKFZ and Universität Heidelberg; J. Bodem, Universität Heidelberg;A. Jacob, J. Hoheisel, DKFZ, Heidelberg

22 Global gene expression response of Corynebacterium glutamicum 55to Inhibitors of cell wall integrityC Stansen. L. Eggeling, V.F. Wendisch, Forschungszentrum Jülich GmbH

23 Protein immobilization in microstructured surface-attached hydrogels 56M. Moschallski. R. Toomey, K. Seidel, J. Rühe, University of Freiburg

24 DNA-microarray genotyping based detection of beta-lactamases 57for clinical microbiologyV. Grimm. University of Stuttgart; M. Susa, C. Knabbe, Robert Bosch Hospital,Stuttgart; R.D. Schmid, T.T. Bachmann, University of Stuttgart

25 Development of a DNA microarray for the detection and 58identification of fungal pathogens involved in invasive mycosisD. Leinberqer. University of Stuttgart; U. Schumacher, I. Autenrieth,University of Tübingen; T.T. Bachmann, University of Stuttgart

26 Optical acetylcholinesterase protein chip for insecticide detection 59K. Kurr. H. Schulze, R.D. Schmid, T.T. Bachmann, University of Stuttgart

27 DNA-microarray-based strain improvement: reduction of glucose 60overflow metabolism in Escherichia coliA. Veit, T. Polen, V.F. Wendisch, Forschungszentrum Jülich GmbH

Posterprogramm

Nr. Seite

28 Plasma aided surface modification for the generation of DNA 61microarraysT. Neumann, J. Rühe, University of Freiburg

29 Dynamic network reconstruction based on gene expression 62data obtained from hepatocytes stimulated by LiCIW. Schmidt-Heck. Hans Knoell Institute for Natural Products Research, Jena;S. Zellmer, F. Gaunitz, University of Leipzig; R. Guthke, Hans Knoell Institute forNatural Products Research, Jena; R. Gebhardt, University of Leipzig

30 Effect of different commonly applied normalization procedures on 63the detection of differentially expressed genes in a typical geneexpression experimentK. Holzmann. M. Buchholz, M. Braun, H.A. Kestler, A. Schwoerer, T.M. Gress,Universitätsklinikum Ulm

31 Rapid detection of resistance determinants and toxins in clinical 64isolates of Staphylococcus aureus using a DNA-array based assayS. Monecke. TU Dresden; R. Ehricht, Clondiag Chip Technologies, Jena

32 Differential gene expression between specialized myofibroblast-like 65cells (stellate cells) and normal fibroblasts: analysis of functionalimplications using Gene Ontology annotationsM. Buchholz, H.A. Kestler, A. Müller, T.M. Gress, University of Ulm

33 Characterization of bacterial response to toxic chemicals using 66DNA microarraysF. Stahl. M. Engel, C Klockow, B. Hitzmann, Universität Hannover; K.F. Reardon,Colorado State University, Fort Collins, CO/USA; T. Scheper, Universität Hannover

34 Nanoparticles and applications in protein-bio-chip technology 67K. Borchers, A. Weber, H. Brunner, G.E.M. Tovar, Fraunhofer-Institute forInterfacial Engineering and Biotechnology and University of Stuttgart

35 Production of custom oligonucleotide microarrays for pharma 68research using a 96 nozzles printheadO. Gutmann1. G. Dernick2, C. Mangold2, M. Tessier2, M. Daub1, B. de Heij1,C. Fattinger2, R. Zengerle1, U. Certa2

1 IMTEK, University of Freiburg2 F. Hoffmann-La Röche AG, Basel/CH

36 Combinatorial antibody microarrays as tools for the analysis of 70T cell activation pathwaysK. Köhler. T. Andre, L. Grosse-Hovest, G. Jung, R. Brock, University of Tübingen

Posterprogramm

Nr. Seite

37 Oligonucleotide microarray for the rapid identification and 71molecular characterization of enterococciB. Strommenqer. C. Kettlitz, G. Werner, W. Witte,Robert Koch-Institute, Wernigerode

38 Development of peptide chips for biomedical applications 72H. Andresen1. K. Zarse1'2, C. Grötzinger2, M. Birringer2, O.J. Kreuzer2,E. Ehrentreich-Förster1, F.F. Bier1

1 Fraunhofer Institut für Biomedizinische Technik IBMT, Nuthetal2 peptides&elephants GmbH, Nuthetal

39 Development of a multiplex OnChip-PCR and their use in 73diagnostic applicationsD. Andresen. E. Ehrentreich-Förster, M. Nickisch-Rosenegk, F.F. Bier,Fraunhofer Institute for Biomedical Engineering, Nuthetal; M. Kuhn,Congen Biotechnology GmbH, Berlin

40 Integrated process control for highly parallel and contact-free 74microarray printingR. Niekrawietz. W. Honstein, O. Gutmann, B. de Heij, R. Zengerle, M. Daub,University of Freiburg

41 Peptide synthesis for microarrays or other applications 76A. Jacob. 0. Brandt, M. Dauber, A. Stephan, DKFZ, Heidelberg; D. Kassahn,H. Illges, Biotechnology Institute Thurgau, Tägerwilen/CH; J. Hoheisel,DKFZ, Heidelberg

42 A cellular microarray for the profiling of T lymphocyte antigen 77specificityA. Hoff. T. Andre, R. Fischer, M. Fotin-Mleczek, S. Voss, University of Tübingen;K.-H. Wiesmüller, EMC microcollections GmbH, Tübingen; R. Brock,University of Tübingen

43 High-density DNA oligonucleotide arrays: influence of 78immobilization chemistry and hybridization conditions onsignal strength and specificityS. Weinhold. University Clinic of Düsseldorf; T. Polen, V. Wendisch, ResearchCentre Jülich; P. Wernet, M. Uhrberg, University Clinic of Düsseldorf

44 Power and specifity of replicated microarray experiments in the 79detection of differential gene expressionJ. Budczies. GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg;F. Hlubek, T. Brabletz, Universität Erlangen; H.-W. Mewes, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg

Posterprogramm

Nr. Seite

45 A device for the generation of 2D-antibody gradients by 81microstructured stampingG. Roth, EMC microcollections GmbH, Tübingen; O. Stoevesandt, K. Köhler,D. Kern, R. Brock, University of Tübingen; K.-H. Wiesmüller,EMC microcollections GmbH, Tübingen

46 Direct detection of chemically labeled prokaryotic mRNA on 82oligonucleotide microarrays for expression profilingC Würdemann. S. Schübbe, A. Ellrott, J. Peplies, D. Schüler, MPI for MarineMicrobiology, Bremen; F.O. Glöckner, MPI for Marine Microbiology, Bremenand International University Bremen

47 Development of a monitoring System for microorganisms in the 83environment using DNA array technologyC. Beckmann. G. Vogel, Kantonales Laboratorium Basel-Stadt/CH;V. Lemee, Zeptosens AG, Witterswil/CH; U. Vögeli, A. Herrmann,Kantonales Laboratorium Basel-Stadt/CH

48 Standardized genome-wide cDNA microarray experiments for 84a comprehensive analysis of gene expression in human diseasesR. Kuner, A. Buness, K. Steiner, J. Schneider, K. Finis, M. Stojanov,M. Ruschhaupt, W. Huber, A. Poustka, H. Sültmann, DKFZ, Heidelberg

49 Miniaturized light sources in the red, green, blue-violet and 85UV (316 nm) wavelength ränge: an optical platform for novelsensors and lab-on-a-chip SystemsT. Gebel, L. Rebohle, T. Trautmann, nanoparc GmbH, Dresden;W. Skorupa, nanoparc GmbH, Dresden and Institute of Ion Beam Physics andMat. Research, Dresden; G. Gauglitz, R. Frank, University of Tübingen

50 Protein microarray analysis by using UV fluorescence spectroscopy: 86detection of antigen-antibody and protein-ligand bindingP. Griqaravicius. P. Schellenberg, H.-M. Striebel, K.O. Greulich, Institute ofMolecular Biotechnology, Jena

51 The Hannover cytokine microarray project of the SFB566 87„Cytokines and cytokine-dependent signalling pathways astherapeutic target structures"O. Dittrich-Breiholz, H. Schneider, A. Weber, M. Kracht, Medical School Hannover

Posterprogramm

Nr. Seite

52 Biomarker discovery and identification in different human tumors 88by ProteinChip arrays and their diagnostic relevanceF. von Eqqelinq. G. Ernst, B. Schimmel, A. Bleul, S.'Koscielny, Friedrich-Schiller-University, Jena; A. Wiesner, R. Bogumil, Ciphergen Biosystems Ltd.,Guildford/UK; K.-J. Halbhuber, U. Möller, C. Meile, Friedrich-Schiller-University,Jena

53 Der „Treg-Chip human": Ein Oligonukleotid-Mikroarray für humane 89regulatorische T-ZellenS. Pförtner. W. Hansen, T. Toepfer, M. Probst-Kepper, J. Buer, R. Geffers,GBF, Braunschweig

54 In vivo ChlP-cloning of novel HNF4a gene targets 90M. Niehof, R. Zemlin, J. Borlak, Fraunhofer Institute of Toxicology andExperimental Medicine, Hannover

55 DualChip™ microarray: a new approach in gene expression analysis 92F. de Lonqueville, V. Bertholet, M. Brasseur, S. Evrard, E. Gancarek,Eppendorf Array Technologies, Namur/B; M. Scheidel, J. Mueller-Ibeler,Eppendorf AG, Hamburg

56 Comprehensive interpretation of transcriptome and proteome data 94of a human pathogenN.C. Hauser1. C Busold2, K. Fellenberg2, J. Hoheisel2, S. Winter3, J. Dippon3,H. Brunner1, S. Rupp1

1 Fraunhofer IGB, Stuttgart2 DKFZ, Heidelberg3 Universität Stuttgart

57 Gene expression profiling of lupus nephritis in a MRL-+/+ mouse 95modelB. Xu, J. Hoheisel, DKFZ, Heidelberg; H. Radeke, Klinikum der UniversitätFrankfurt; A. Schwarting, Universitätsklinik Mainz; M. Frohme, DKFZ, Heidelberg

58 Libraries of Random External Controls (LOREC) for applications 96in microarray experimentsA. Koschmieder. M. Frohme, DKFZ, Heidelberg

59 The Hydractinia echinata EST and functional genome analysis project 97J. Soza Ried. DKFZ, Heidelberg; U. Frank, University of Heidelberg; H. Bode,University of California, Irvine, CA/USA; M. Frohme, DKFZ, Heidelberg

Posterprogramm

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60 Strukturierte BioChips zur Einzelzell-Analytik 98T. Zacher. K. Hagen-Mann, U. Schön, W. Mann, Alopex GmbH, Kulmbach

61 System-on-Chip: Möglichkeiten der modernen Mikroelektronik 99M. Glesner. O. Soffke, T. Hollstein, H. Zimmer, Technische UniversitätDarmstadt

62 Simultaneous detection and identification of microorganisms 100by electrical biochipsA. Breitenstein. H. Zinecker, S. Bau, H. Maucher, Scanbec GmbH, Halle

Last Minute Posters (nach Programmdruck)

63 A new planar support for multiple affmity enrichment 101U. Beutling, K. Bialek, V. Gupte, A. Swistowski, R. Rübenhagen, R. Frank,GBF, Braunschweig

64 Isolation of highly specific antibodies from polyclonal sera for 102Sandwich antibody microarraysR. Rübenhagen, R. Frank, GBF, Braunschweig

65 Optimizing hybridization on cDNA microarrays using the 103SlideBooster Hyb-StationA. Toegl, R. Kirchner, M. Kantlehner, A. Zuleger, Advalytix AG, Brunnthal

66 Development of a microarray to monitor the expression of 104trichothecen biosynthetic genes in FusariaM. Schmidt-Hevdt. R. Geisen,Bundesforschungsanstalt für Ernährung und Lebensmittel, Karlsruhe

67 The EU projekt Micropad: microarrays for the detection of Protozoa, 105flagelated Algae and DiatomsK. Metfies. L.K. Mediin, Alfred Wegener Institute for Polar and MarineResearch, Bremerhaven; G. Muyzer, C. Mayer, P. Roosken, Delft Universityof Technology/NL; M. Berzano, R. Spurio, C. Gualerzi, University of Camerino/I

68 Development of a DNA microchip as a Standard analytical tool for the 106identification of phytoplanktonC. Gescher. K. Metfies, L.K. Mediin,Alfred Wegener Institute for Polar and Marine Research, Bremerhaven

Posterprogramm

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69 Biomolecular processing modules - new concepts for configurable 107nano-scale biochemistryP.F. Wagler2-3. U. Tangen13, T. Maeke1'3, M. Jünger1, S. Chemnitz1, S. Ehses2,T. Ruecker1, J.S. McCaskill1'3

1 Ruhr-Universität Bochum2 Fraunhofer Gesellschaft, St. Augustin3 ProtoStream GmbH, St. Augustin

70 Hybrid biochemical chip devices fabricated by PCB and 109micromachining technologiesS. Chemnitz1, M. Jünger1, T. Maeke13, T. Palutke2, U. Tangen1'3, P.F. Wagler23

1 Ruhr-Universität Bochum2 Fraunhofer Gesellschaft, St. Augustin3 ProtoStream GmbH, St. Augustin

71 Application of DNA microarrays for immunotoxicological testing: 111identification of sensitising chemicals and discrimination betweencontact allergens and respiratory allergensS. Szameit. M. Mansfeld, W. Novak, H. Tuschl, C. Nöhammer,ARC Seibersdorf research GmbH/A

72 Definition of a microarray-based platform for the detection of 112breast cancer cellsK. Vierlinqer. ARC Seibersdorf research GmbH/A; K.H. Preisegger,EccoCell Biotechnologie GmbH, Graz/A; C Nöhammer,ARC Seibersdorf research GmbH/A

73 DNA microarrays for identification of infectious bacteria and 113their antibiotic resistanceH. Wiesinqer-Mavr, R. Pichler, ARC Seibersdorf research GmbH/A;L.M. Simma, University Hospital of Vienna/A; L. Bodrossy, ARC Seibersdorfresearch GmbH/A; E. Presterl, A.M. Hirschl, University Hospital of Vienna/A;C. Nöhammer, ARC Seibersdorf research GmbH/A

74 Combined proteome and tränscriptome analysis of alterations 114in the mouse brain caused by Huntington's diseaseC. Hultschig1. C. Zabel2, M. Blüggel3, G.P. Bates4, H.E. Meyer5, H. Eickhoff6,S. Kloska6, H. Lehrach1, J. Klose2

1 Max-Planck Institut für Molekulare Genetik, Berlin2 Universitätsklinikum Charite, Berlin3 Protagen AG, Dortmund4 Guy's Hospital, London/UK5 Ruhr-Universität Bochum6 Scienion AG, Berlin