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Supplemental Figure 4 a b 10 20 40 80 1000 10000 100000 LM8 Dunn (cell number/well) 6 hr CellTiter -Glo R.L.U. measurement 10% FBS DMEM Collagen 1 gel (2.4 mg/ml) Collagen 1 gel (2.4, 1.2 mg/ml) 1000, 2000, 4000, 8000 cells/well into 96 well dish 7 days CellTiter -Glo R.L.U. measurement 10% FBS DMEM Collagen 1 gel (2.4 mg/ml) 10, 20, 40, 80 cells/well into 96 well dish Luminescence (R.L.U.) (cell number/well) collagen concentrati on Luminescence (R.L.U.) 1000 2000 4000 8000 100 1000 10000 LM8 2.4 LM8 1.2 Dunn 2.4 Dunn 1.2 (mg/ ml)

Supplemental Figure 4

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Supplemental Figure 4. a. 1000, 2000, 4000, 8000 cells/well into 96 well dish. 10, 20, 40, 80 cells/well into 96 well dish. R.L.U. measurement. R.L.U. measurement. CellTiter-Glo. CellTiter-Glo. 10% FBS DMEM. 10% FBS DMEM. 7 days. 6 hr. co llagen concentration. - PowerPoint PPT Presentation

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Page 1: Supplemental Figure 4

Supplemental Figure 4

a

b

10 20 40 801000

10000

100000

LM8

Dunn

(cell number/well)

6 hr

CellTiter-Glo

R.L.U. measurement

10% FBS DMEM

Collagen 1 gel (2.4 mg/ml)

Collagen 1 gel (2.4, 1.2 mg/ml)

1000, 2000, 4000, 8000 cells/well into 96 well dish

7 days

CellTiter-Glo

R.L.U. measurement

10% FBS DMEM

Collagen 1 gel (2.4 mg/ml)

10, 20, 40, 80 cells/well into 96 well dish

Lum

ines

cenc

e (R

.L.U

.)

(cell number/well)

collagenconcentration

Lum

ines

cenc

e (R

.L.U

.)

1000 2000 4000 8000100

1000

10000

LM8 2.4

LM8 1.2

Dunn 2.4

Dunn 1.2 (mg/ml)

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