T4O14 - GHOSH

Embed Size (px)

Citation preview

  • 8/3/2019 T4O14 - GHOSH

    1/40

    Scientific Evaluation of AromaticScientific Evaluation of Aromatic--cumcum--MedicinalMedicinal

    Grasses for the Welfare of Marginal PeopleGrasses for the Welfare of Marginal PeopleUplifting Rural Economy in theUplifting Rural Economy in the GangeticGangetic Plains ofPlains of

    Eastern IndiaEastern India

    EX-Associate Professor & Head, Department of Botany

    Bejoy Narayan College, Itachuna, Hooghly, West Bengal

    Secretary General, Indian Society for Promotion of

    Medicinal & Aromatic Plants (ISMAP)

    Regional Representative of International

    Council of Medicinal & Aromatic Plants (ICMAP)

    And Advisor and consultant of Medicinal & Aromatic Plants.

    E-mail : [email protected]

    BYBY

    M. Sc. (Gold Medallist), Ph. D.

  • 8/3/2019 T4O14 - GHOSH

    2/40

    INTRODUCTION

    > In recent times, herbal plants are finding diverse uses as raw materials,not only for medicines but also as biopesticides and many other articles of

    cosmetic and perfumery value like soaps, shampoos, talcum powder,

    toothpaste and aroma compounds used in food flavours and fragrances of

    natural colours in the world. Global demand for herbal products has steadilyincreased and there has been tremendous developmental activities within

    the industrial sectors to develop various types of herbal products.

    > The appropriate selection of a herb for specific purpose, its taxonomic

    identification, cultivation practice and harvesting at appropriate time aresome of the steps necessary for ensuring a supply of quality raw materials

    for production of high valued herbal products. Since, medicinal and

    aromatic plants are new and uncommon to the growers, they are unaware of

    the fact that these are to be maintained. As the demand for domestic andexport market has been increasing, the production of these crops on a large

    scale by incorporating them into cropping system of the country has

    become necessary to maintain sustainable production and supply.

  • 8/3/2019 T4O14 - GHOSH

    3/40

    Medicinal and Aromatic plants are now on the decline due to deforestation, lack of

    scientific cultivation, conservation etc. This is a problem that demands our

    immediate attention for greater interest for the nation.It is urgent need to follow the following important recommendations made by

    the third and fourth world congress on Medicinal and Aromatic Plants

    (WOCMAP) and the International council of Medicinal and Aromatic Plants

    (ICMAP) :

    1. Should bring together information on identity, distribution and conservationstatus of MAPs.

    2. Should gather information on how they are used including local traditional

    knowledge.

    3. Should gather information on the extent to which they are harvested in wild

    population.4. Should gather information on their cultivation & propagation.

    5. Should gather information on their agronomy and cultivation in protected

    area.

    6. Ethno veterinary medicine as well as the use of plants and plant products to

    improve animal health, productivity and welfare requires more internationattention.

    7. Economic sustainability of plant procurement through wild collection,

    domestication and cultivation must be secured through transparent costing

    and prizing that will provide ample opportunities for rural income

    generation and community development.

  • 8/3/2019 T4O14 - GHOSH

    4/40

    The State of West Bengal is gifted with an enormous wealth of diversity in

    plant species. These can be a priceless resource with high potential for

    economic and ecological gains especially in the rural areas if properly protectedand promoted. The area of the District of West Bengal (Hooghly) where the

    present investigation has been carried out is inhabited by marginal poor

    people and a large area of land, which is dry, upland and some what

    unfertilized not suitable for traditional crop rice cultivation, is left out as

    waste land.Considering the importance of the recommendations of the WOCMAP & ICMAP and

    in order to preserve the natural resources of the MAPs of the area along with

    their sustainable use and their commercial exploitation for the welfare of the

    rural people, present author has attempted to carry out the following

    investigations for survey & conservation: Survey of the natural resources of MAPs in the area.

    Proper identification of the species growing locally and their uses by the

    local people.

    Observation to know the causes of depletion and loss of the plants.

    Conservation strategies of MAPs. Agro-technology and economics of cultivation for scientific growing of a

    few medicinal and aromatic plants in the area.

    Promotion of MAPs in the area.

  • 8/3/2019 T4O14 - GHOSH

    5/40

    An attempt has also been made to standardize all the factors

    responsible for growth and development of some selected

    commercially viable economically important aromatic

    grasses and make them feasible for large scale cultivation at

    the cultivators field after providing the know how ofcultivation. In a separate line of study, efforts have been

    made to increase growth and developmental pattern of the

    grasses including the biomass production by using optimum

    fertilizers, photoperiodic stimulus and bio-regulators with

    special reference to the synthesis of essential oil along with

    quality. Finally, analysis of economics of cultivation of the

    grasses has been made for their prospect of commercialexploitation in the gangetic plains of West Bengal.

    Ultimately this will help the diversification of crop pattern

    and uplift the rural economy.

  • 8/3/2019 T4O14 - GHOSH

    6/40

    > Role of Environment , Growth & Developmental physiology> Role of Environment , Growth & Developmental physiology

    for the synthesis of secondary metabolites:for the synthesis of secondary metabolites:The Chemical compounds responsible for aroma are the secondary

    metabolites produced in plant parts. Although Genes are responsible for

    the production of these compounds but the expression of Genes is

    controlled by environmental factors and growth and development patternof a particular plant.

    According to Meyer and Anderson, the environment is the foster parent

    of every plant and plays an indispensable role in its development as do

    hereditary factors which are the Biological Parents.Genetic Constituent +

    Environment

    Internal Processes and

    condition regulated by

    hormones & enzymes

    Organic

    Development

    (Growth, Photo

    synthetic activity,

    Translocation and

    accumulation of

    necessary raw

    materials)

  • 8/3/2019 T4O14 - GHOSH

    7/40

    MATERIALS :MATERIALS :1) Cymbopogon flexuosus Steud. Wats. (Lemon Grass)

    2) Cymbopogon martini Stapf. (Palmarosa)

    3) Cymbopogon winterianus Jowitt. (Citronella)

    Family : Poaceae.

    Following Experiments have been done:Following Experiments have been done:

    Cultural Experiments :Cultural Experiments :

    Sowing & Transplanting Experiment

    Fertilizer Experiments (Different combinations of NPK):Fertilizer Experiments (Different combinations of NPK):N1P1K1 = N60 P50 K30kg & N2P2K2=N120P100 K60 kg./ha.

    Photoperiodic Experiments:Photoperiodic Experiments:

    Long days & short days experiment (-10,20 and 30PIC)

    Hormonal Experiments :Hormonal Experiments :

    GA3 50g./ml. and 100 g./ml.

  • 8/3/2019 T4O14 - GHOSH

    8/40

    The different developmental phases were distinguished on theThe different developmental phases were distinguished on the

    Basis of presence of reproductive units as follows:Basis of presence of reproductive units as follows:

    1.Vegetative : From germination stage upto initiation of bud.

    2.Reproductive : From the initiation of bud upto approximately

    20% bud, 70% flowers and 10% fruits.

    3.Post-reproductive : After reproductive phase, upto 95% fruits and

    5% flowers.

    4.Senile : After post-reproductive phase, upto the periodwhen 75% of the leaves yellowed.

  • 8/3/2019 T4O14 - GHOSH

    9/40

    Following detailed studies have been undertaken:Following detailed studies have been undertaken:

    1. Growth and Developmental behaviour of the plants under

    above mentioned experiments

    2. Essential oil synthesis in response to the above mentioned

    experiments during different developmental phases.3. Analysis of some important metabolites connected with

    E.oil synthesis.

    4. Analysis of major chemical constituents of the oils duringdifferent developmental phases.

    5. Correlationship among growth, development,metabolism

    and essential oil formation.

    6. Analysis of yield of herbage & oil per hectare

    7. Economics of cultivation of the plants.

  • 8/3/2019 T4O14 - GHOSH

    10/40

    West Bengal

    Latitude: 22 34' North

    Longitude: 88 22' East

  • 8/3/2019 T4O14 - GHOSH

    11/40

    METEOROLOGICAL DATAMETEOROLOGICAL DATA

    (During the period of Experimentation)

    Temperature RelativeHumidity

    (%)

    Months

    Max. Min Max Min.

    TotalRainfall(mm)

    Rainydays(No.)

    Duration ofBright

    Sunshine(hr.)

    January

    February

    March

    April

    May

    June

    July

    August

    September

    October

    November

    December

    24.6

    29.1

    33.0

    35.2

    34.6

    32.6

    31.9

    32.8

    32.7

    31.9

    29.2

    31.1

    10.2

    13.0

    18.5

    22.9

    25.0

    25.5

    25.7

    25.7

    25.5

    22.9

    16.0

    10.5

    95

    95

    96

    91

    89

    92

    94

    91

    96

    96

    96

    97

    41

    42

    46

    53

    63

    79

    82

    82

    75

    75

    64

    49

    0.25

    7.15

    48.8

    46.1

    125.9

    267.0

    304.6

    278.4

    270.5

    200.0

    19.7

    4.2

    1

    2

    3

    5

    8

    16

    22

    21

    14

    10

    4

    1

    9.7

    9.5

    9.5

    10.5

    11.0

    5.8

    5.5

    6.5

    8.2

    9.6

    9.5

    9.0

    Soil Condition : Clay Soil, pH-6.

    Soil Composition: N2-4%, P(as P2O5) - 0.03%, Ca (as CaO) - 0.01% and K-0.04%.

    In the Met. data of the

    area, max. temp. was

    noted from March toAugust. Increase in the

    max. and min. level of

    relative humidity with

    their min. variation was

    noted during June to

    Sept. Whereas,increase in the duration

    of bright sunshine was

    noted from October to

    May.

    Soil condition and soil

    composition as

    given in the bottom of

    the table.

  • 8/3/2019 T4O14 - GHOSH

    12/40

    Results of Survery and Conservation of Medicinal and Aromatic Plants in Gangetic Bengal

    Table I

    Medicinal Herbs (locally available) used by the common people for curing common ailments

    Common Diseases Herbs used Parts used

    Cold & CoughCold & CoughCold & CoughCold & Cough Achyranthes aspera Seeds

    Allium cepa Scales

    Ocimum sanctum Leaves

    Zingiber officinale Rhizome

    Adhatoda vasica Leaves

    Datura stramonium Seeds

    Body PainsBody PainsBody PainsBody Pains Aloe vera Leaves

    Zingiber officinale Rhizome

    Allium cepa Bulb

    Allium sativum Bulb

    Argemone mexicana Roots

    Solanum nigrum Leaves

    Centella asiatica Leaves

    Ocimum sanctum Leaves

    Calotropis procera Leaves

  • 8/3/2019 T4O14 - GHOSH

    13/40

    Alimentary Tract ProblemsAlimentary Tract ProblemsAlimentary Tract ProblemsAlimentary Tract Problems Aegle marmelos Fruit pulp & leaves

    Allium sativum BulbPiper longum Fruits

    Piper betle Leaves

    Emblica officinalis Fruits

    Cassia fistula Ripe FruitsCentella asiatica Leaves

    Phyllanthus niruri Whole plant

    Andrographis paniculata Leaves

    Enhydra fluctuens Leaves

    Hygrophila spinoca Leaves

    MemoryMemoryMemoryMemory Bacopa monnieri Leaves

    Withania somnifera Roots

    Vitex negunda Leaves

    DiabetesDiabetesDiabetesDiabetes Phyllanthus niruri Whole plant

    Emblica officinalis Fruits

    Catharanthas roseus Leaves

    Gymnema sylvestris Leaves

  • 8/3/2019 T4O14 - GHOSH

    14/40

    Kidney StonesKidney StonesKidney StonesKidney Stones Beorhaavia diffusa Leaves

    Bryophyllum calycium Leaves

    Phyllanthus niruri Whole plant

    Dolichos biflorus Seeds

    Liver DiseasesLiver DiseasesLiver DiseasesLiver Diseases Andrographis paniculata Leaves

    Tinospora cordifolia Stems

    Solanum nigrum Leaves & Fruits

    Alstonia scholaris Bark

    Hair ProblemHair ProblemHair ProblemHair Problem Elipta alba Leaves

    Emblica officinalis Fruits

    Azadirachta indica Leaves & Fruits

    Skin ProblemSkin ProblemSkin ProblemSkin Problem Aloe vera Leaves

    Azadirachta indica Leaves & Fruits

    Curcuma longa Rhizome

    Cuts & WoundsCuts & WoundsCuts & WoundsCuts & Wounds Cynodon Dactylon Whole Plant

    Tagetis patula Leaves

    Azadirachta indica Leaves

    ImmunityImmunityImmunityImmunity Asparagus racemosus Root Powder

    Withania Somnifera Root Powder

  • 8/3/2019 T4O14 - GHOSH

    15/40

    Table II

    Gradually depleting Medicinal and Aromatic Plants

    Scientific name Common name Causes of Depletion

    ___________________________________________________________________________

    *1.Acacia arobica Babul Natural & Biotic

    *2.Adhatoda vasica Vasaka Natural & Biotic

    *3.Aegle marmelos Bel Biotic

    *4.Allium cepa Onion Biotic

    *5. Allium sativum Garlic Biotic

    *6.Aloe vera GritaKumari Natural

    *7.Alstonia scholaris Chatim Biotic

    *8. Andrographis paniculata Kalmegh Natural

    *9.Asperagus racemosus Satamuli Biotic*10.Azadirachta indica Neem Biotic

    *11.Bacopa monnieri Brahmi Natural

    *12.Boerhaavia diffusa Punarnova Natural

    *13.Bryophyllum calycinum Patharkuchi Natural

    *14.Catharanthus roseus Nayantara Natural & Biotic

    *15.Centella asiatica Thankuni Natural & Biotic*16.Cissus quandrangularis Harjora Biotic

    *17.Clerodendron infortunatum Ghetu Biotic

    *18.Curcuma longa Halud Biotic

    *19.Cymbopogon flexuosus Lemon grass Natural & Biotic

  • 8/3/2019 T4O14 - GHOSH

    16/40

    *20.Dolichos biflorus Kulatta kalai Biotic

    *21.Eupatorium triplinerve Ayapana Natural

    *22.Gloriosa superba Ulotchandal Natural*23. Gymnema sylvestris Gudmar Natural & Biotic

    *24.Holarrhena antidysentrica Kurchi Biotic

    *25.Hygrophila spinosa Kulekhara Natural & Biotic

    *26. Justicia rubra Bisallakarani Biotic

    *27. Mentha piperita Pudina Natural

    *28.Mimosops elangi Bakul Biotic*29.Mimosa pudica Lajjabati Natural & Biotic

    *30.Nelumbo nucifera Padma Natural & Biotic

    *31.Ocimum sanctum Radhatulsi Natural & Biotic

    *32.Phyllanthus embelica Amlaki Biotic

    *33.Rauvolfia serpentina Sarpagandha Natural & Biotic

    *34.Saraka indica Ashok Biotic

    *35.Terminalia arjuna Arjun Biotic

    *36.Tinospora cordifolia Gulancha Natural & Biotic

    *37.Tylophora indica Anantamul Natural & Biotic

    *38.Vitex negunda Nishinda Biotic

    *39.Wedelia calendula Keshraj Natural & Biotic*40.Withania somnifera Aswagandha Natural & Biotic

  • 8/3/2019 T4O14 - GHOSH

    17/40

    Table III

    Agro-technology of Medicinal & Aromatic Plants for proper conservation & Cultivation (in brief)

    Aromatic Plants

    Name Habit Soil Propagation Time of

    plan

    ting

    &

    spa

    cing

    Fertilizer Harvesting

    Time

    Main

    Chemi

    cals

    Net Profit

    (Rs./

    ha)

    & Parts

    used

    Cymbopogon flexuosusvar.OD-19(Ita-1)

    (Lemon Grass)

    Perennialarom

    atic

    grass

    Well drainedsandy

    loam

    Seeds or rootedslips

    Rainyseas

    on

    60cm x

    60c

    m

    Composed-120q/ha.

    NPK- 60:50:40

    Kg/ha

    4 cuttingsfrom

    2nd year

    onward

    s upto 5

    yr.

    Citral (75-90%)

    45,000/-(Leaves)

    Cymbopogon winterianus

    var.Manjusha

    (Citronella)

    Perennial

    arom

    atic

    grass

    Light loamy

    soil or

    sandy

    soil

    Rooted slips Rainy

    seas

    on

    60cm x

    60c

    m

    Vermi

    Compos

    ed-

    150q/ha.

    NPK-

    150:60:6

    0 kg/ha

    4 cuttings

    from

    2nd year

    onward

    s upto 5

    yr.

    Geraniol

    (90%)

    &

    Cironel

    lal (35-

    45%)

    40,000/-

    (Leaves)

    Mentha piperita

    (Pudina/Pepermint)

    Perennial

    arom

    atic

    crop

    Light

    calcare

    ous or

    deep

    richloams

    Suckers &

    stolons

    Winter

    seas

    on

    50cm x

    50cm

    Composed-

    100q/ha.

    NPK-

    100:60:5

    0 Kg/ha

    2 cuttings in 6

    months

    Menthol (50-

    55%)

    30,000/-

    (Leaves)

    Ocimum basilicum

    (Sweet Basil)

    Erect

    peren

    nial

    under

    -

    shrub

    Well drained

    rich

    loamy

    with

    alkalin

    e-

    acidicsoil.

    Seeds Rainy

    seas

    on

    60cm x

    60c

    m

    Composed-

    120q/ha.

    NPK-

    120:60:4

    0 Kg/ha

    Full bloom

    stage

    then 2

    months

    interval

    s

    Linalool (50-

    60%)

    80,000/-

    (Leaves)

  • 8/3/2019 T4O14 - GHOSH

    18/40

    Medicinal Plants

    Name Habit Soil Propagation Time of plan

    ting

    &

    spac

    ing

    Fertilizer HarvestingTime MainChe

    mica

    ls

    Net Profit(Rs./

    ha)

    & Parts

    used

    Andrographis paniculata

    (Kalmegh)

    Erect annual

    herb

    Clayey to

    sandy

    soil

    Seeds or

    cutting

    s

    June-July.

    30c

    m x

    15cm

    Composed-

    30 q/ha.

    NPK-

    30:20:10

    Kg/ha

    Just before

    flow

    ering

    stage

    Kalmeghin,

    And

    rogr

    aphin &

    Pani

    colin

    60,000/-

    (Leaves)

    Adhatoda vasica (Vasaka) Erect

    peren

    nial

    under

    shrub

    Moist sandy

    &

    alluvia

    l

    Steam

    cutting

    s

    Rainy

    seas

    on

    1mx

    1m

    Bone dust-

    50q/h

    a.

    NPK-

    50:30:20/h

    a

    After 6

    mont

    hs

    Quinozole

    Vaci

    colin

    ,Adh

    atodi

    ne,

    etc.

    50,000/-

    (Leaves)

    Centella asiatica (Thankuni) Prostrate

    peren

    nial

    creepi

    ng

    herb

    Natural moist

    soil

    Rooted node

    cutting

    s

    Rainy

    seas

    on

    20cm x

    20c

    m

    Vermi

    Comp

    osed-

    30q/h

    a.

    3-4 harvests

    per

    year

    Glucoside-

    asiat

    icosi

    de &

    Asia

    tic

    asid

    30,000/-

    (Leaves)

    Catharanthus rosesus

    (Periwinkle)

    Erect

    peren

    nial

    herb

    High alkaline

    loamy

    soil

    Seeds &

    steam

    cutting

    s

    Rainy

    seas

    on

    60c

    mx

    60cm

    Composed-

    50q/h

    a.

    NPK-

    50:30

    :20kg/ha

    After 10-12

    mont

    hs

    Alkaloids

    Vinb

    lasti

    ne &

    Vinc

    ristine

    75,000/-

    (Roots &

    Leav

    es)

    Table - IV

  • 8/3/2019 T4O14 - GHOSH

    19/40

    Results of Growth andDevelopmental Physiology

    in relation to the synthesis ofEssential oils and Economics

    of cultivation of Aromatic

    Grasses in Gangetic Bengal

  • 8/3/2019 T4O14 - GHOSH

    20/40

    Table - V

    Effect of effective treatments on leaf formation, dry weight accEffect of effective treatments on leaf formation, dry weight accumulation andumulation and

    essential oil formation in lemon grass andessential oil formation in lemon grass and palmarosapalmarosa during vegetative phase.during vegetative phase.

    In the table, analysis of growth behaviour and E.oil formation of the plants clearly revealed

    that max. rates of extension growth, leaf formation and dry wt. accum. remained

    associated with the max. rate of formation of E.oil. It has been also observed that the most

    effective treatments of fertilizer, photoperiod and GA3 increased the growth behaviour

    thereby increasing the E.oil formation. Max. effect was noted in N1P1K1 treatment.

    % increase (+) or decrease (-) over control

    Treatments Leaf formation Dry wt. accumulation Essential oil formation

    CF CM CF CM CF CM

    N1P1K1 +25 +40 +15 +14 +16 +25

    LD-20 PIC +12 +26 +06 +07 +10 +13

    SD-20 PIC -02 -22 -02 -03 -01 -08

    GA3-50g/ml +08 +16 +04 +05 +07 +09

    CF : C. flexuosus, CM : C. martini, N1 = 60 kg/ha., P1 = 50 kg/ha., K1 = 30 kg/ha.,

    PIC = Photo Inductive Cycle.

  • 8/3/2019 T4O14 - GHOSH

    21/40

    Table - VI

    Duration of developmental phases and corresponding essential oilDuration of developmental phases and corresponding essential oil

    formation in different treatments in lemon grass andformation in different treatments in lemon grass and palmarosapalmarosa..

    Our results have clearly established a very definitive role of reproductive period on essential oil

    formation and the data of fertilizer, photoperiod and gibberellin treatments have lucidly exposed to

    a more congenial internal environment during reproductive growth for an augmented production

    of essential oil per unit amount of laminar tissue.

    CF : C. flexuosus, CM : C. martini, N1 = 60 kg/ha., P1 = 50 kg/ha., K1 = 30 kg/ha.,

    PIC = Photo Inductive Cycle, --- no flower formation.

    Treatments Duration (days) of phases E. Oil (gm./100 gm) content

    Vegetative Reproductive Vegetative Reproductive

    CF CM CF CM CF CM CF CM

    Control 105 75 45 60 0.30 0.36 0.36 0.40

    N1P1K1 93 60 57 80 0.41 0.48 0.48 0.52

    N1P1 95 62 55 73 0.38 0.46 0.46 0.52

    N1K1 100 65 50 70 0.36 0.44 0.40 0.48

    SD-10 PIC 100 80 50 50 0.31 0.32 0.37 0.36

    LD-20 PIC --- 63 --- 67 --- 0.40 --- 0.45

    GA3-50g/ml 117 65 38 65 0.34 0.38 0.39 0.43

  • 8/3/2019 T4O14 - GHOSH

    22/40

    Table VII

    Duration of senile phase and essential oil content in lemon grasDuration of senile phase and essential oil content in lemon grass ands and palmarosapalmarosa..

    In the table, it has been noted that shorter the duration of senile phase higher is theessential oil content in the treated plants as compared to control. In NPK treated

    plants the minimum duration of senile phase is closely associated with the maximum

    essential oil content.

    CF : C. flexuosus, CM : C. martini, N1 = 60 kg/ha., P1 = 50 kg/ha., K1 = 30 kg/ha.,

    PIC = Photo Inductive Cycle.

    Treatments Increase (+) or decrease (-) of senile

    phase over control (days)

    % increase (+) or decrease (-) of

    essential oil content over control

    C-flexuosus C. martini C-flexuosus C. martini

    N1P1K1 -10 -10 +0.09 +0.08

    N1P1 -06 -08 +0.07 +0.07

    N1K1 -05 -05 +0.03 +0.03

    LD-10 PIC +05 -05 +0.03 +0.03

    GA3-50g/ml +05 -06 +0.05 +0.04

  • 8/3/2019 T4O14 - GHOSH

    23/40

    Table - VIII

    Synthesis of essential oil in relation to total nitrogen, solublSynthesis of essential oil in relation to total nitrogen, soluble nitrogen and total carbohydratee nitrogen and total carbohydrate

    formation in different treatments during developmental phases offormation in different treatments during developmental phases oflemon grass andlemon grass and palmapalma rosarosa..

    Of the three Biochemical parameters, the increase of soluble Nitrogen and total Carbohydrate contents

    could be said to remain associated with increased synthesis of E.oil during reproductive phase. It might be said

    that increasing accum. of Carbohydrate, mainly derived from the accum. of Photosynthates, could serve as one ofthe ingredients to be channeled in to the formation of E.oil precursor.

    Veg. : Vegetative Phase, Ref : Reproductive Phase.

    Treatments % content during phases

    Total nitrogen Soluble nitrogen Total Carbohyd Essential oil

    Veg. Rep. Veg. Rep. Veg. Rep. Veg. Rep.

    C-flexuosus

    Control 2.36 2.26 0.80 1.26 2.41 3.03 0.30 0.36

    N1P1K1 2.96 2.92 1.10 1.51 3.10 3.32 0.41 0.48

    LD-20 PIC 2.48 2.49 0.81 1.36 2.58 3.15 0.36 0.40

    GA3-50g/ml 2.50 2.45 0.84 1.30 2.44 3.08 0.34 0.39

    C-martini

    Control 2.44 2.28 0.90 1.30 2.28 3.16 0.36 0.40

    N1P1K1 3.06 3.04 1.14 1.48 2.52 3.66 0.48 0.52

    LD-20 PIC 2.68 2.52 0.98 1.40 2.40 3.40 0.40 0.45

    GA3-50g/ml 2.56 2.35 0.92 1.35 2.32 3.28 0.38 0.43

  • 8/3/2019 T4O14 - GHOSH

    24/40

    Table - IX

    Formation of essential oil under varying effective treatments duFormation of essential oil under varying effective treatments during differentring differentdevelopmental phases of lemon grass anddevelopmental phases of lemon grass and palmarosapalmarosa..

    In this table, it is observed that the total content of the E.oil per unit laminar tissue,

    when examined separately during vegetative, reproductive, post reproductive and

    senile phases, always appeared to be max. during reproductive phase and N1P1K1treatment showed max. content than the other treatments.

    CF : C. flexuosus, CM : C. martini, N-only = Nitrogen 60kg/ha.

    Optimum

    treatments

    Essential oil content (gm/100 gm fresh laminar tissue)

    Vegetative Reproductive Post reproduct. Senile

    CF CM CF CM CF CM CF CM

    Control 0.30 0.36 0.36 0.40 0.30 0.38 0.25 0.32

    N1P1K1 0.41 0.48 0.48 0.52 0.40 0.45 0.34 0.40

    N-only 0.35 0.42 0.42 0.50 0.38 0.42 0.32 0.38

    LD-20 PIC 0.31 0.45 0.40 0.48 0.36 0.43 0.30 0.38

    GA3-50g/ml 0.34 0.38 0.39 0.46 0.34 0.42 0.30 0.36

  • 8/3/2019 T4O14 - GHOSH

    25/40

    Table - X

    CitralCitral andand GeraniolGeraniol content during different developmental phases ofcontent during different developmental phases oflemon grass andlemon grass and palmarosapalmarosa in different treatmentsin different treatments

    While analysing citral & Geraniol contents in lemon grass & pamarosa oils during the

    progress of developmental phases of the plants, it was revealed that the chemical

    constituents attained its max. peak during reproductive phase of development. Max.

    content was noted in N1P1K1 treatment.

    C = Citral, G = Geraniol

    Optimum

    treatments

    % Content during phases

    Vegetative Reproductive Post reproduct. Senile

    C G C G C G C G

    Control 66.80 66.42 80.00 85.00 70.00 70.00 76.00 62.00

    N1P1K1 72.50 70.00 88.80 90.00 78.80 80.32 80.00 70.00

    LD-20 PIC 68.00 68.00 82.40 88.00 72.00 78.00 76.00 64.00

    GA3-50g/ml 68.00 66.00 80.80 82.00 70.00 70.00 72.00 52.00

  • 8/3/2019 T4O14 - GHOSH

    26/40

    Table XI

    *Yield of green herbage and*Yield of green herbage and E.oilE.oil inin C.C. flexuosusflexuosus (CF) and(CF) and

    C. martiniC. martini(CM) under varying optimum treatments(CM) under varying optimum treatments

    Treatments Yield of herbage (quintal/hectare) Yield of E.oil (kg/hectare)

    C-flexuosus C. martini C-flexuosus C. martini

    Transplanted plants 250-300 200-250 110-120 100-110

    N1P1K1 500-550 350-400 250-260 170-180

    LD-20 PIC 300-350 250-300 130-140 110-120

    * Average of 5 years (upto six cuttings in C-flexuosus and four cuttings in C. martini

    Table XII

    CF CM

    Total cost of Cultivation including distillation (Rs.) 55000.00 45000.00

    Total Return (Rs.) 110000.00 90000.00

    Net Gain (Rs.)/ha. 55000.00 45000.00

    Economics of cultivation of lemonEconomics of cultivation of lemon grass(CFgrass(CF) and) and

    PalmarosaPalmarosa (CM) /hectare (Average of 5 years)(CM) /hectare (Average of 5 years)

  • 8/3/2019 T4O14 - GHOSH

    27/40

    CONCLUSION1. About 400 plants which are available in the area are of medicinal value.2. About 80 MAPs are widely used by the local people to cure their common diseases.

    3. About 40 MAPs which are depleting day to day have been recorded and these are conserved

    in the Medicinal Plant Garden as Ex situ conservation.

    4. Biotic pressure (including the over exploitation, excess cultivation of traditional crops,

    unscientific collection by the suppliers and destruction of the plants for the development of

    buildings i.e. urbanization) and Natural factors (including climatic change, drought, loss ofwater bodies etc) are the chief for the depletion and gradual loss of MAPs.

    5. Specific requirements of agronomical input and also other requirements like physical and

    chemical treatments for success in cultivation of the MAPs in the gangetic tract of West

    Bengal.

    6. Relation between weather condition of the area (bright sunshine, higher variation of

    maximum & minimum temperature and humidity) and essential oil formation.7. Relation between growth and essential oil synthesis of the plants.

    8. Definite role of reproductive phase for increased synthesis of essential oils & major

    chemical constituents.

    9. Morphological and Biochemical indexing of the essential oil formation.

    10. Increased production of herbage yield and oil yield per hectare towards different optimum

    treatments and net gain for cultivation of the plants would be attractive to the farmers toexploit the plants (specially the aromatic grasses) commercially in the area.

    11. The aromatic-cum-medicinal plants may be exploited as non-traditional cash in the

    Gangetic tract of West Bengal for the upliftment of rural economy.

  • 8/3/2019 T4O14 - GHOSH

    28/40

    SUGGESTIONGovernment or private agencies should

    take initiative and active interest in

    1) Conservation, development and cultivation practices of important

    medicinal and aromatic plants.

    2) Expansion of the area under cultivation to meet the growing domestic as

    well as export demand and reclamation of fallow land.3) Generation of quality planting material through the development of herbal

    gardens and nurseries and using modern biotechnology.

    4) Establishment of processing and distillation units in suitable places with

    easy access for the rural people.

    5) Expansion and dissemination of Agro Technology, as developed, to therural people and user agencies through local Panchayats and Block

    Development Offices.

    6) Establishment of training centres to train the rural people for the cultivation

    of MAPs and extraction of essential oils of aromatic crops.

    7) Establishment of Co-operative societies through which the raw products

    developed by the rural people can be easily sold directly to the users.

    8) Establishment of a coordinated network among the different segments

    involved in the development of aromatic crops, especially the scientists, \

    farmers, traders and the Government and non-Government organizations

    for sustainable utilization of MAPs for the welfare of rural masses.

  • 8/3/2019 T4O14 - GHOSH

    29/40

    Promotion of MAPs in the area has been done by organizing :

    1. By organizing national symposium (NACMAP-05)

    2. Local seminars & workshops.3. Training programmes in our institution and in a rural technology centre

    for the sake of farmers and unemployed youths.

    4. Awareness programme in Schools & Colleges to make the local people

    aware about the importance of the conservation of MAPs in the area

    and also make them interested to grow the crops as nontraditional cashcrops in their fallow land (not suitable for traditional rice cultivation)

    with the agro technology developed in our institution, which will

    ultimately enable them to earn extra money and enhance their job

    opportunities

    I owe my sincere gratitude to Prof. Dr. S. K. Chatterjee, Dr. C. M. Ghosh,

    West Bengal, India and

    Prof. Dr. Ch. Franz (Austria) and Dr. L.E. Craker (USA)

    for sending invaluable massage for my promotional activities of MAPs

    in the remote area for uplifting rural economy.

  • 8/3/2019 T4O14 - GHOSH

    30/40

    ACKNOWLEDGEMENTThe Author is grateful to the following Scientists /

    Organizations for their Help and Encouragement :

    1.Prof. Dr. S. K. Chatterjee (Ex. Prof. and Head Deptt. of Botany, B.U.,

    Director and other med. Plants, Govt. of West Bengal ; M.D., W.B.

    Pharmaceutical & Phyto Chemical Development Corp Ltd. India).

    2.Prof. Dr. Ch. Franz, Director, Institute of Applied Botany and FoodScience, Vienna, Austria & Vice-President, ICMAP.

    3.Dr. S. Sahoo, Scientist, R.R.L., Bhubaneswar, India.

    4.Dr. D. D. Patra, Scientist, CIMAP, Lucknow, India

    5.Dr. Chintamoni Ghosh, Director, State Medicinal Plants Board, W.B.,

    6.U.G.C., New Delhi, India.

    7.D.S.T., Govt. of West Bengal,India

    8.Department of Agri Horticulture Society of India, Kolkata

    9.Principal, B.N. Mahavidyalaya, Itachuna, Hooghly, W.B., India for

    providing the research plots and necessary laboratory facilities.10.Director, Rural Technology centre, Burdwan University, India.

  • 8/3/2019 T4O14 - GHOSH

    31/40

    LET US TAKE A QUICK LOOK AT THE PHOTOGRAPHSLET US TAKE A QUICK LOOK AT THE PHOTOGRAPHS

    Lemon Grass

  • 8/3/2019 T4O14 - GHOSH

    32/40

    Lemongrass Field

    Palmarosa(high yielding var.)

    Palmarosa Field

  • 8/3/2019 T4O14 - GHOSH

    33/40

    Citronella OcimumMenthaLemon Grass

    Centella CatharanthusAndrographis AdhatodaAdhatoda

  • 8/3/2019 T4O14 - GHOSH

    34/40

    Research Lab. Extraction

    With Dr. Kubeckza

    (Germany) With Dr. Franz(Vienna)

  • 8/3/2019 T4O14 - GHOSH

    35/40

    THAILAND

    WOCMAP-III

    WITH DR. HEYWOOD(THAILAND)

    WOCMAP -III

    WITH EOAI SECRETARY

  • 8/3/2019 T4O14 - GHOSH

    36/40

    WITH DR. KHANJUJA

    CIMAP WITH EOAI PRECIDENT

    WITH DR. S.K. CHATTERJEENACMAP- 2005

  • 8/3/2019 T4O14 - GHOSH

    37/40

    WOCMAP- IV (CAPETOWN)

  • 8/3/2019 T4O14 - GHOSH

    38/40

    Work Shop

    Training Programme Consultancy

    With Dr. S.K. Chatterkee

    Principal Speaker

  • 8/3/2019 T4O14 - GHOSH

    39/40

    In conclusion, it is worth mentioning thatthe urgent need of the hour is the sincerecooperation and coordination among

    scientists, policy makers, traders and farmersinstrumental for the successful promotionand development of medicinal and aromatic

    plants in a particular agroclimatic regionwith appropriate agrotechnology fulfillingthe needs of domestic as well as exportdemand of herbal products on one hand andreclamation of wasteland thereby upliftingrural economy on the other.

  • 8/3/2019 T4O14 - GHOSH

    40/40

    THANK YOU