www.impactjournals.com/oncotarget/ Oncotarget, Supplementary Materials 2016

The antinociceptive effects of ferulic acid on neuropathic pain: involvement of descending monoaminergic system and opioid receptors

Supplementary Materials

Supplementary Figure S1: (A) The effects of repeated pre-administration of 6-OHDA (5 μg and 10 μg per mouse) on mechanical allodynia and thermal hyeralgesia in CCI mice. (B) The effects of repeated pre-administration of PCPA (50 and 150 mg/kg) on mechanical allodynia and thermal hyeralgesia in CCI mice. Results are expressed as meanSEM from 8 mice. *p < 0.05 vs. vehicle-treated sham group.

Supplementary Figure S2: (A) The effects of repeated pre-administration of alpha-AR antagonist phentolamine (0.5 and 1.5 mg/kg) on mechanical threshold in CCI mice. (B) The effects of repeated pre-administration of beta-AR antagonist propranolol (0.5 and 1.5 mg/kg) on mechanical threshold in CCI mice. Results are expressed as meanSEM from 8 mice. *p < 0.05 vs. vehicle-treated sham group.

Supplementary Figure S3: (A) The effects of repeated pre-administration of beta1-AR antagonist metoprolol (5 and 10 mg/kg) on mechanical allodynia in CCI mice. (B) The effects of repeated pre-administration of beta2-AR antagonist ICI118,551 (0.5 and 1 mg/kg) on mechanical allodynia in CCI mice. (C) The effects of repeated co-administration of beta3-AR antagonist SA 59230A (0.5 and 1 mg/kg) on mechanical allodynia in CCI mice. Results are expressed as mean ± SEM from 8 mice. *p < 0.05 vs. vehicle-treated sham group.

Supplementary Figure S4: (A) The effects of repeated pre-administration of 5-HT1A antagonist WAY-100635 (0.1 and 0.5 mg/kg) on thermal hyeralgesia in sham and CCI mice. (B) The effects of repeated pre-administration of 5-HT1B antagonist isamoltane (0.5 and 1 mg/kg) on thermal hyeralgesia in CCI mice. (C) The effects of repeated pre-administration of 5-HT2A/2C antagonist ritanserin (1 and 2 mg/kg) on thermal hyeralgesia in CCI mice. (D) The effects of repeated pre-administration of 5-HT3 antagonist ondansetron (0.05 and 0.1 mg/kg) on thermal hyeralgesia in CCI mice. Results are expressed as mean ± SEM from 8 mice. *p < 0.05 vs. vehicle-treated sham group.