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LAPORAN BIOKIMIA Makassar, 14 November 2012 BIOMEDIK 1 BIOINFORMATIKA NAMA : MUHAMAD SHUBHY ( 110 212 0020) MUAMAR GHIFFARY (110 212 0068) WAHYUNI SAPAN (110 212 0060) SUDARMINI (110 212 0044) INDRY PRIANDINI B. (110 212 0012) SHERLY NOVIANA R. (110 212 0004) MUNADYA HAMJA (110 212 0006) IRFAN RINALDI (110 212 0052) IKA PRATIWI L. (110 212 0028) INDRA LUKMANA (110 210 0023) KELOMPOK : 1 A FAKULTAS KEDOKTERAN

Tugas Bioinformatika Kelompok 1a

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Page 1: Tugas Bioinformatika Kelompok 1a

LAPORAN BIOKIMIA Makassar, 14 November 2012

BIOMEDIK 1

BIOINFORMATIKA

NAMA : MUHAMAD SHUBHY ( 110 212 0020)

MUAMAR GHIFFARY (110 212 0068)

WAHYUNI SAPAN (110 212 0060)

SUDARMINI (110 212 0044)

INDRY PRIANDINI B. (110 212 0012)

SHERLY NOVIANA R. (110 212 0004)

MUNADYA HAMJA (110 212 0006)

IRFAN RINALDI (110 212 0052)

IKA PRATIWI L. (110 212 0028)

INDRA LUKMANA (110 210 0023)

KELOMPOK : 1 A

FAKULTAS KEDOKTERAN

UNIVERSITAS MUSLIM INDONESIA

MAKASSAR

2012

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Homo sapiens pro-melanin-concentrating hormone (PMCH), mRNA

Ini merupakan mRNA karena ada polieteil dan tidak ada batas antar exon (tidak adaintron)

Nomor Sequnce : NM_002674.2

1 agcaggctga cagagaaatt tttcattttc tttcttgttt gactgtatgc aaacatcaaa 61 ctaagaatgg caaaaatgaa tctctcttcc tatatattaa tactaacttt ttctttgttt 121 tctcaaggta ttttactttc agcatccaag tccataagaa atttagatga tgacatggta 181 tttaatacat tcaggttggg gaaaggcttt cagaaggaag acactgcaga aaaatcagtt 241 attgctcctt ccctggaaca atataaaaat gatgagagca gtttcatgaa cgaagaggaa 301 aataaagttt caaagaacac aggctccaaa cataatttct taaatcatgg tctgccactg 361 aatctggcta taaaacctta tcttgcacta aaaggatctg tagctttccc agctgagaat 421 ggagttcaga atactgaatc aacacaagaa aagagagaaa ttggggatga agaaaactca 481 gctaaatttc ctataggaag gagagatttt gacatgctca gatgtatgct gggaagagtc 541 taccgacctt gttggcaagt ctgatacctg ttggtccaca tcatcttttc agaagaaaat 601 aaaagcattt aattgccaat gggaggagaa gcccatactg ctactataac ttgtgtatgt 661 taaatgtctg ttttaaaaga aagtagtgtt aagatgtatc agtaactgaa atgatatgct 721 ttctctgtgc attaaacttt gtgaaaattc tgcataa

Gen :

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Exon :

1 agcaggctga cagagaaatt tttcattttc tttcttgttt gactgtatgc aaacatcaaa 61 ctaagaatgg caaaaatgaa tctctcttcc tatatattaa tactaacttt ttctttgttt 121 tctcaaggta ttttactttc agcatccaag tccataagaa atttagatga tgacatggta 181 tttaatacat tcaggttggg gaaaggcttt cagaaggaag acactgcaga aaaatcagtt 241 attgctcctt ccctggaaca atataaaaat gatgagagca gtttcatgaa cgaagaggaa 301 aataaagttt caaagaacac aggctccaaa cataatttct taaatcatgg tctgccactg 361 aatctggcta taaaacctta tcttgcacta aaaggatctg tagctttccc agctgagaat 421 ggagttcaga atactgaatc aacacaagaa aagagagaaa ttggggatga agaaaactca 481 gctaaatttc ctataggaag gagagatttt gacatgctca gatgtatgct gggaagagtc 541 taccgacctt gttggcaagt ctgatacctg ttggtccaca tcatcttttc agaagaaaat 601 aaaagcattt aattgccaat gggaggagaa gcccatactg ctactataac ttgtgtatgt 661 taaatgtctg ttttaaaaga aagtagtgtt aagatgtatc agtaactgaa atgatatgct 721 ttctctgtgc attaaacttt gtgaaaattc tgcataa

Exon 1

Exon 2

Exon 3

Graphich

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Keterangan :

Exon:  exon 1Title:  /number=1

inference=alignment:SplignLocation: 1..315Length:  315

Exon:  exon 3Title:  /number=3

inference=alignment:SplignLocation: 515..757Length:  243

Fasta

>gi|71361683|ref|NM_002674.2| Homo sapiens pro-melanin-concentrating hormone (PMCH), mRNAAGCAGGCTGACAGAGAAATTTTTCATTTTCTTTCTTGTTTGACTGTATGCAAACATCAAACTAAGAATGGCAAAAATGAATCTCTCTTCCTATATATTAATACTAACTTTTTCTTTGTTTTCTCAAGGTATTTTACTTTCAGCATCCAAGTCCATAAGAAATTTAGATGATGACATGGTATTTAATACATTCAGGTTGGGGAAAGGCTTTCAGAAGGAAGACACTGCAGAAAAATCAGTTATTGCTCCTTCCCTGGAACAATATAAAAATGATGAGAGCA

Exon:  exon 2Title:  /number=2

inference=alignment:SplignLocation: 316..514Length:  199

Gene:  PMCHTitle:  pro-melanin-concentrating

hormoneLocation: 1..757Length:  757

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GTTTCATGAACGAAGAGGAAAATAAAGTTTCAAAGAACACAGGCTCCAAACATAATTTCTTAAATCATGGTCTGCCACTGAATCTGGCTATAAAACCTTATCTTGCACTAAAAGGATCTGTAGCTTTCCCAGCTGAGAATGGAGTTCAGAATACTGAATCAACACAAGAAAAGAGAGAAATTGGGGATGAAGAAAACTCAGCTAAATTTCCTATAGGAAGGAGAGATTTTGACATGCTCAGATGTATGCTGGGAAGAGTCTACCGACCTTGTTGGCAAGTCTGATACCTGTTGGTCCACATCATCTTTTCAGAAGAAAATAAAAGCATTTAATTGCCAATGGGAGGAGAAGCCCATACTGCTACTATAACTTGTGTATGTTAAATGTCTGTTTTAAAAGAAAGTAGTGTTAAGATGTATCAGTAACTGAAATGATATGCTTTCTCTGTGCATTAAACTTTGTGAAAATTCTGCATAA

Gen Scan (perbandingan dengan genbank) :

Di genbank 1 gene saja dan 3 exon, sedangkan di genscane terbaca 2 gene dan tidak ada exon

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BALST

MENCARI KESAMAAN SEQUNCE NUKLEOTIDA DENGAN SPESIES LAIN (TIKUS)

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Hasil Translate Sequence FASTA PMCH Di Expasy

5'3' Frame 1SRLTEKFFIFFLV-LYANIKLRMAKMNLSSYILILTFSLFSQGILLSASKSIRNLDDDMVFNTFRLGKGFQKEDTAEKSVIAPSLEQYKNDESSFMNEEENKVSKNTGSKHNFLNHGLPLNLAIKPYLALKGSVAFPAENGVQNTESTQEKREIGDEENSAKFPIGRRDFDMLRCMLGRVYRPCWQV-YLLVHIIFSEENKSI-LPMGGEAHTATITCVC-MSVLKESSVKMYQ-LK-YAFSVH-TL-KFCI

5'3' Frame 2AG-QRNFSFSFLFDCMQTSN-EWQK-ISLPIY-Y-LFLCFLKVFYFQHPSP-EI-MMTWYLIHSGWGKAFRRKTLQKNQLLLLPWNNIKMMRAVS-TKRKIKFQRTQAPNIIS-IMVCH-IWL-NLILH-KDL-LSQLRMEFRILNQHKKREKLGMKKTQLNFL-EGEILTCSDVCWEESTDLVGKSDTCWSTSSFQKKIKAFNCQWEEKPILLL-LVYVKCLF-KKVVLRCISN-NDMLSLCIKLCENSA-

5'3' Frame 3QADREIFHFLSCLTVCKHQTKNGKNESLFLYINTNFFFVFSRYFTFSIQVHKKFR--HGI-YIQVGERLSEGRHCRKISYCSFPGTI-K--EQFHERRGK-SFKEHRLQT-FLKSWSATESGYKTLSCTKRICSFPS-EWSSEY-INTRKERNWG-RKLS-ISYRKERF-HAQMYAGKSLPTLLASLIPVGPHHLFRRK-KHLIANGRRSPYCYYNLCMLNVCFKRK-C-DVSVTEMICFLCALNFVKILH

3'5' Frame 1LCRIFTKFNAQRKHIISVTDTS-HYFLLKQTFNIHKL--QYGLLLPLAIKCFYFLLKR-CGPTGIRLANKVGRLFPAYI-ACQNLSFL-EI-LSFLHPQFLSFLVLIQYSELHSQLGKLQILLVQDKVL-PDSVADHDLRNYVWSLCSLKLYFPLRS-NCSHHFYIVPGKEQ-LIFLQCLPSESLSPT-MY-IPCHHLNFLWTWMLKVKYLEKTKKKLVLIYRKRDSFLPFLV-CLHTVKQERK-KISLSAC

3'5' Frame 2YAEFSQSLMHRESISFQLLIHLNTTFF-NRHLTYTSYSSSMGFSSHWQLNAFIFF-KDDVDQQVSDLPTRSVDSSQHTSEHVKISPSYRKFS-VFFIPNFSLFLC-FSILNSILSWESYRSF-CKIRFYSQIQWQTMI-EIMFGACVL-NFIFLFVHETALIIFILFQGRSNN-FFCSVFLLKAFPQPECIKYHVII-ISYGLGC-K-NTLRKQRKS-Y-YIGREIHFCHS-FDVCIQSNKKENEKFLCQPA

3'5' Frame 3MQNFHKV-CTEKAYHFSY-YILTLLSFKTDI-HTQVIVAVWASPPIGN-MLLFSSEKMMWTNRYQTCQQGR-TLPSIHLSMSKSLLPIGNLAEFSSSPISLFSCVDSVF-TPFSAGKATDPFSAR-GFIARFSGRP-FKKLCLEPVFFETLFSSSFMKLLSSFLYCSREGAITDFSAVSSF-KPFPNLNVLNTMSSSKFLMDLDAESKIP-ENKEKVSINI-EERFIFAILSLMFAYSQTRKKMKNFSVSL

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HASIL BLAST DI GENBANK

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BLAST ALIGN TWO SEQUNCES

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Homo sapiens chemokine (C-C motif) ligand 26 (CCL26), mRNA

NCBI Reference Sequence: NM_006072.4

LOCUS NM_006072 562 bp mRNA linear PRI 10-NOV-2012DEFINITION Homo sapiens chemokine (C-C motif) ligand 26 (CCL26), mRNA.ACCESSION NM_006072VERSION NM_006072.4 GI:51944958KEYWORDS .SOURCE Homo sapiens (human)

ORIGIN 1 ctggaattga ggctgagcca aagaccccag ggccgtctca gtctcataaa aggggatcag 61 gcaggaggag tttgggagaa acctgagaag ggcctgattt gcagcatcat gatgggcctc 121 tccttggcct ctgctgtgct cctggcctcc ctcctgagtc tccaccttgg aactgccaca 181 cgtgggagtg acatatccaa gacctgctgc ttccaataca gccacaagcc ccttccctgg 241 acctgggtgc gaagctatga attcaccagt aacagctgct cccagcgggc tgtgatattc 301 actaccaaaa gaggcaagaa agtctgtacc catccaagga aaaaatgggt gcaaaaatac 361 atttctttac tgaaaactcc gaaacaattg tgactcagct gaattttcat ccgaggacgc 421 ttggaccccg ctcttggctc tgcagccctc tggggagcct gcggaatctt ttctgaaggc 481 tacatggacc cgctggggag gagagggtgt ttcctcccag agttacttta ataaaggttg 541 ttcatagagt tgacttgttc at

HIGHLIGHT SEQUENCE FEATURES

A. GENE

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B. EXON

1 ctggaattga ggctgagcca aagaccccag ggccgtctca gtctcataaa aggggatcag 61 gcaggaggag tttgggagaa acctgagaag ggcctgattt gcagcatcat gatgggcctc 121 tccttggcct ctgctgtgct cctggcctcc ctcctgagtc tccaccttgg aactgccaca 181 cgtgggagtg acatatccaa gacctgctgc ttccaataca gccacaagcc ccttccctgg 241 acctgggtgc gaagctatga attcaccagt aacagctgct cccagcgggc tgtgatattc 301 actaccaaaa gaggcaagaa agtctgtacc catccaagga aaaaatgggt gcaaaaatac 361 atttctttac tgaaaactcc gaaacaattg tgactcagct gaattttcat ccgaggacgc 421 ttggaccccg ctcttggctc tgcagccctc tggggagcct gcggaatctt ttctgaaggc 481 tacatggacc cgctggggag gagagggtgt ttcctcccag agttacttta ataaaggttg 541 ttcatagagt tgacttgttc at

KETERANGAN :

: EXON 1

: EXON 2

: EXON 3

: EXON 4

C. GRAPHIC

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1. EXON 1

exon: exon 1

Title: /number=1 inference=alignment:Splign

Location: 1..30

Length: 30

2. EXON 2

3. EXON 3

exon: exon 3

Title: /number=3 inference=alignment:Splign

Location: 182..296

Length: 115

EXON

GEN

CDS

exon: exon 2

Title: /number=2 inference=alignment:Splign

Location: 31..181

Length: 151

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4. EXON 4

exon: exon 4

Title: /number=4 inference=alignment:Splign

Location: 297..562

Length: 266

GENE :

Gene: CCL26Title: chemokine (C-C motif) ligand 26

Location: 1..562

Length: 562

CDS :

CDS: NP_006063.1

Title: C-C motif chemokine 26 precursor

Location: 109..393

Length: Span: 285

Product: 94

D. FASTA

>gi|51944958|ref|NM_006072.4| Homo sapiens chemokine (C-C motif) ligand 26 (CCL26), mRNACTGGAATTGAGGCTGAGCCAAAGACCCCAGGGCCGTCTCAGTCTCATAAAAGGGGATCAGGCAGGAGGAGTTTGGGAGAAACCTGAGAAGGGCCTGATTTGCAGCATCATGATGGGCCTCTCCTTGGCCTCTGCTGTGCTCCTGGCCTCCCTCCTGAGTCTCCACCTTGGAACTGCCACACGTGGGAGTGACATATCCAAGACCTGCTGCTTCCAATACAGCCACAAGCCCCTTCCCTGGACCTGGGTGCGAAGCTATGAATTCACCAGTAACAGCTGCTCCCAGCGGGCTGTGATATTCACTACCAAAAGAGGCAAGAAAGTCTGTACCCATCCAAGGAAAAAATGGGTGCAAAAATACATTTCTTTACTGAAAACTCCGAAACAATTGTGACTCAGCTGAATTTTCATCCGAGGACGCTTGGACCCCGCTCTTGGCTCTGCAGCCCTCTGGGGAGCCTGCGGAATCTTTTCTGAAGGCTACATGGACCCGCTGGGGAGGAGAGGGTGTTTCCTCCCAGAGTTACTTTAATAAAGGTTGTTCATAGAGTTGACTTGTTCAT

E. GENSCAN

View gene model output: PS | PDFGENSCAN 1.0 Date run: 14-Nov-112 Time: 00:23:30

Sequence /tmp/11_14_12-00:23:30.fasta : 609 bp : 52.01% C+G : Isochore 3 (51 - 57 C+G%)

Parameter matrix: HumanIso.smat

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Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..

----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

1.01 Init + 156 293 138 2 0 80 45 176 0.532 10.61

Suboptimal exons with probability > 1.000

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..

----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

NO EXONS FOUND AT GIVEN PROBABILITY CUTOFF

Predicted peptide sequence(s):

>/tmp/11_14_12-00:23:30.fasta|GENSCAN_predicted_peptide_1|46_aa

MMGLSLASAVLLASLLSLHLGTATRGSDISKTCCFQYSHKPLPWTW

F. EXPASY TRANSLATE TOOL

Translate Tool - Results of translation

Please select one of the following frames:

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5'3' Frame 1

XXXXXXXXXXXXXXXXWN-G-AKDPRAVSVS-KGIRQEEFGRNLRRA-FAAS-WASPWPLLCSWPPS-VSTLELPHVGVTYPRPAASNTATSPFPGPGCEAMNSPVTAAPSGL-YSLPKEARKSVPIQGKNGCKNTFLY-KLRNNCDSAEFSSEDAWTPLLALQPSGEPAESFLKATWTRWGGEGVSSQSYFNKGCS-S-LVH

5'3' Frame 2

XXXXXXXXXXXXXXXTGIEAEPKTPGPSQSHKRGSGRRSLGET-EGPDLQHHDGPLLGLCCAPGLPPESPPWNCHTWE-HIQDLLLPIQPQAPSLDLGAKL-IHQ-QLLPAGCDIHYQKRQESLYPSKEKMGAKIHFFTENSETIVTQLNFHPRTLGPRSWLCSPLGSLRNLF-RLHGPAGEERVFPPRVTLIKVVHRVDLF

5'3' Frame 3

XXXXXXXXXXXXXXXLELRLSQRPQGRLSLIKGDQAGGVWEKPEKGLICSIMMGLSLASAVLLASLLSLHLGTATRGSDISKTCCFQYSHKPLPWTWVRSYEFTSNSCSQRAVIFTTKRGKKVCTHPRKKWVQKYISLLKTPKQL-LS-IFIRGRLDPALGSAALWGACGIFSEGYMDPLGRRGCFLPELL--RLFIELTCS

3'5' Frame 1

MNKSTL-TTFIKVTLGGNTLSSPAGPCSLQKRFRRLPRGLQSQERGPSVLG-KFS-VTIVSEFSVKKCIFAPIFSLDGYRLSCLFW--ISQPAGSSCYW-IHSFAPRSREGACGCIGSSRSWICHSHVWQFQGGDSGGRPGAQQRPRRGPS-CCKSGPSQVSPKLLLPDPLL-D-DGPGVFGSASIPXXXXXXXXXXXXXXXX

3'5' Frame 2

-TSQLYEQPLLK-LWEETPSPPQRVHVAFRKDSAGSPEGCRAKSGVQASSDENSAESQLFRSFQ-RNVFLHPFFPWMGTDFLASFGSEYHSPLGAAVTGEFIASHPGPGKGLVAVLEAAGLGYVTPTCGSSKVETQEGGQEHSRGQGEAHHDAANQALLRFLPNSSCLIPFYETETALGSLAQPQFQXXXSXXXXXXXXXXX

3'5' Frame 3

EQVNSMNNLY-SNSGRKHPLLPSGSM-PSEKIPQAPQRAAEPRAGSKRPRMKIQLSHNCFGVFSKEMYFCTHFFLGWVQTFLPLLVVNITARWEQLLLVNS-LRTQVQGRGLWLYWKQQVLDMSLPRVAVPRWRLRREARSTAEAKERPIMMLQIRPFSGFSQTPPA-SPFMRLRRPWGLWLSLNSSXRXHXXXXXXXXXXX

G. BLAST1) BLAST P (ONE SEQUENCE)

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2) BLAST P (TWO OR MORE SEQUENCE)

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H. PROSITE

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Homo sapiens proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) (PSMB8), transcript variant 2, mRNA

NCBI Reference Sequence: NM_148919.3

1 ttcctcctcc gagagcggac agatctctgg gtgctgggcg gtcatggcgc tactagatgt 61 atgcggagcc ccccgagggc agcggccgga atcggctctc ccggttgcgg gaagcgggcg 121 tcgctcggac ccaggacact acagtttctc tatgcgatct ccagagctcg ctttaccccg 181 gggaatgcag cccacagaat tcttccagtc cctgggtggg gacggagaaa ggaacgttca 241 gattgagatg gcccatggca ccaccacgct cgccttcaag ttccagcatg gagtgattgc 301 agcagtggat tctcgggcct cagctgggtc ctacattagt gccttacggg tgaacaaggt 361 gattgagatt aacccttacc tgcttggcac catgtctggc tgtgcagcag actgtcagta 421 ctgggagcgc ctgctggcca aggaatgcag gctgtactat ctgcgaaatg gagaacgtat 481 ttcagtgtcg gcagcctcca agctgctgtc caacatgatg tgccagtacc ggggcatggg 541 cctctctatg ggcagtatga tctgtggctg ggataagaag ggtcctggac tctactacgt 601 ggatgaacat gggactcggc tctcaggaaa tatgttctcc acgggtagtg ggaacactta 661 tgcctacggg gtcatggaca gtggctatcg gcctaatctt agccctgaag aggcctatga 721 ccttggccgc agggctattg cttatgccac tcacagagac agctattctg gaggcgttgt 781 caatatgtac cacatgaagg aagatggttg ggtgaaagta gaaagtacag atgtcagtga 841 cctgctgcac cagtaccggg aagccaatca ataatggtgg tggtggcagc tgggcaggtc 901 tcctctggga ggtcttggcc gactcaggga cctaagccac gttaagtcca aggagaagaa 961 gaggcctagc ctgagccaaa gagagagtac gggctcagca gccagaggag gccggtgaag 1021 tgcatcttct gcgtgttctc tatttgaaca agcatttccc ccagggaagt ttctgggtgc 1081 cccactaagt agaataaaga aaaacggtta taaataaaaa aaaaaaaaaa aaaaa

Exon

1 ttcctcctcc gagagcggac agatctctgg gtgctgggcg gtcatggcgc tactagatgt 61 atgcggagcc ccccgagggc agcggccgga atcggctctc ccggttgcgg gaagcgggcg 121 tcgctcggac ccaggacact acagtttctc tatgcgatct ccagagctcg ctttaccccg 181 gggaatgcag cccacagaat tcttccagtc cctgggtggg gacggagaaa ggaacgttca 241 gattgagatg gcccatggca ccaccacgct cgccttcaag ttccagcatg gagtgattgc 301 agcagtggat tctcgggcct cagctgggtc ctacattagt gccttacggg tgaacaaggt 361 gattgagatt aacccttacc tgcttggcac catgtctggc tgtgcagcag actgtcagta 421 ctgggagcgc ctgctggcca aggaatgcag gctgtactat ctgcgaaatg gagaacgtat 481 ttcagtgtcg gcagcctcca agctgctgtc caacatgatg tgccagtacc ggggcatggg 541 cctctctatg ggcagtatga tctgtggctg ggataagaag ggtcctggac tctactacgt 601 ggatgaacat gggactcggc tctcaggaaa tatgttctcc acgggtagtg ggaacactta 661 tgcctacggg gtcatggaca gtggctatcg gcctaatctt agccctgaag aggcctatga 721 ccttggccgc agggctattg cttatgccac tcacagagac agctattctg gaggcgttgt 781 caatatgtac cacatgaagg aagatggttg ggtgaaagta gaaagtacag atgtcagtga 841 cctgctgcac cagtaccggg aagccaatca ataatggtgg tggtggcagc tgggcaggtc 901 tcctctggga ggtcttggcc gactcaggga cctaagccac gttaagtcca aggagaagaa 961 gaggcctagc ctgagccaaa gagagagtac gggctcagca gccagaggag gccggtgaag 1021 tgcatcttct gcgtgttctc tatttgaaca agcatttccc ccagggaagt ttctgggtgc 1081 cccactaagt agaataaaga aaaacggtta taaataaaaa aaaaaaaaaa aaaaa

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EXON 1EXON 2EXON 3EXON 4EXON 5EXON 6

GENE

1 ttcctcctcc gagagcggac agatctctgg gtgctgggcg gtcatggcgc tactagatgt 61 atgcggagcc ccccgagggc agcggccgga atcggctctc ccggttgcgg gaagcgggcg 121 tcgctcggac ccaggacact acagtttctc tatgcgatct ccagagctcg ctttaccccg 181 gggaatgcag cccacagaat tcttccagtc cctgggtggg gacggagaaa ggaacgttca 241 gattgagatg gcccatggca ccaccacgct cgccttcaag ttccagcatg gagtgattgc 301 agcagtggat tctcgggcct cagctgggtc ctacattagt gccttacggg tgaacaaggt 361 gattgagatt aacccttacc tgcttggcac catgtctggc tgtgcagcag actgtcagta 421 ctgggagcgc ctgctggcca aggaatgcag gctgtactat ctgcgaaatg gagaacgtat 481 ttcagtgtcg gcagcctcca agctgctgtc caacatgatg tgccagtacc ggggcatggg 541 cctctctatg ggcagtatga tctgtggctg ggataagaag ggtcctggac tctactacgt 601 ggatgaacat gggactcggc tctcaggaaa tatgttctcc acgggtagtg ggaacactta 661 tgcctacggg gtcatggaca gtggctatcg gcctaatctt agccctgaag aggcctatga 721 ccttggccgc agggctattg cttatgccac tcacagagac agctattctg gaggcgttgt 781 caatatgtac cacatgaagg aagatggttg ggtgaaagta gaaagtacag atgtcagtga 841 cctgctgcac cagtaccggg aagccaatca ataatggtgg tggtggcagc tgggcaggtc 901 tcctctggga ggtcttggcc gactcaggga cctaagccac gttaagtcca aggagaagaa 961 gaggcctagc ctgagccaaa gagagagtac gggctcagca gccagaggag gccggtgaag 1021 tgcatcttct gcgtgttctc tatttgaaca agcatttccc ccagggaagt ttctgggtgc 1081 cccactaagt agaataaaga aaaacggtta taaataaaaa aaaaaaaaaa aaaaa

GRAPHIC

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FASTA

>gi|73747874|ref|NM_148919.3| Homo sapiens proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) (PSMB8), transcript variant 2, mRNATTCCTCCTCCGAGAGCGGACAGATCTCTGGGTGCTGGGCGGTCATGGCGCTACTAGATGTATGCGGAGCCCCCCGAGGGCAGCGGCCGGAATCGGCTCTCCCGGTTGCGGGAAGCGGGCGTCGCTCGGACCCAGGACACTACAGTTTCTCTATGCGATCTCCAGAGCTCGCTTTACCCCGGGGAATGCAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGACGGAGAAAGGAACGTTCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTCCAGCATGGAGTGATTGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCCTTACGGGTGAACAAGGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGTGCAGCAGACTGTCAGTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTGCGAAATGGAGAACGTATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGCCAGTACCGGGGCATGGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGTCCTGGACTCTACTACGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACGGGTAGTGGGAACACTTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGCCCTGAAGAGGCCTATGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGCTATTCTGGAGGCGTTGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAAAGTACAGATGTCAGTGACCTGCTGCACCAGTACCGGGAAGCCAATCAATAATGGTGGTGGTGGCAGCTGGGCAGGTCTCCTCTGGGAGGTCTTGGCCGACTCAGGGACCTAAGCCACGTTAAGTCCAAGGAGAAGAAGAGGCCTAGCCTGAGCCAAAGAGAGAGTACGGGCTCAGCAGCCAGAGGAGGCCGGTGAAGTGCATCTTCTGCGTGTTCTCTATTTGAACAAGCATTTCCCCCAGGGAAGTTTCTGGGTGCCCCACTAAGTAGAATAAAGAAAAACGGTTATAAATAAAAAAAAAAAAAAAAAAAA

Hasil genscan terdapat 1248 bp dan genbank 1135 bp

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Hasil Translate Fasta

5'3' Frame 1XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSSESGQISGCWAVMALLDVCGAPRGQRPESALPVAGSGRRSDPGHYSFSMRSPELALPRGMQPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ-WWWWQLGRSPLGGLGRLRDLSHVKSKEKKRPSLSQRESTGSAARGGR-SASSACSLFEQAFPPGKFLGAPLSRIKKNGYK-KKKKKK

5'3' Frame 2XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPPPRADRSLGAGRSWRY-MYAEPPEGSGRNRLSRLREAGVARTQDTTVSLCDLQSSLYPGECSPQNSSSPWVGTEKGTFRLRWPMAPPRSPSSSSME-LQQWILGPQLGPTLVPYG-TR-LRLTLTCLAPCLAVQQTVSTGSACWPRNAGCTICEMENVFQCRQPPSCCPT-CASTGAWASLWAV-SVAGIRRVLDSTTWMNMGLGSQEICSPRVVGTLMPTGSWTVAIGLILALKRPMTLAAGLLLMPLTETAILEALSICTT-RKMVG-K-KVQMSVTCCTSTGKPINNGGGGSWAGLLWEVLADSGT-ATLSPRRRRGLA-AKERVRAQQPEEAGEVHLLRVLYLNKHFPQGSFWVPH-VE-RKTVINKKKKKK

5'3' Frame 3XXXXXXXXXXXXXXXXXYXXXXXXXXXXXXXXXXXXXFLLRERTDLWVLGGHGATRCMRSPPRAAAGIGSPGCGKRASLGPRTLQFLYAISRARFTPGNAAHRILPVPGWGRRKERSD-DGPWHHHARLQVPAWSDCSSGFSGLSWVLH-CLTGEQGD-D-PLPAWHHVWLCSRLSVLGAPAGQGMQAVLSAKWRTYFSVGSLQAAVQHDVPVPGHGPLYGQYDLWLG-EGSWTLLRG-TWDSALRKYVLHG-WEHLCLRGHGQWLSA-S-P-RGL-PWPQGYCLCHSQRQLFWRRCQYVPHEGRWLGESRKYRCQ-PAAPVPGSQSIMVVVAAGQVSSGRSWPTQGPKPR-VQGEEEA-PEPKREYGLSSQRRPVKCIFCVFSI-TSISPREVSGCPTK-NKEKRL-IKKKKKK

3'5' Frame 1FFFFFFLFITVFLYST-WGTQKLPWGKCLFK-RTRRRCTSPASSGC-ARTLSLAQARPLLLLGLNVA-VPESAKTSQRRPAQLPPPPLLIGFPVLVQQVTDICTFYFHPTIFLHVVHIDNASRIAVSVSGISNSPAAKVIGLFRAKIRPIATVHDPVGISVPTTRGEHIS-EPSPMFIHVVESRTLLIPATDHTAHREAHAPVLAHHVGQQLGGCRH-NTFSISQIVQPAFLGQQALPVLTVCCTARHGAKQVRVNLNHLVHP-GTNVGPS-GPRIHCCNHSMLELEGERGGAMGHLNLNVPFSVPTQGLEEFCGLHSPG-SELWRSHRETVVSWVRATPASRNRESRFRPLPSGGSAYI--RHDRPAPRDLSALGGGXXXXXXXXXXXXXKXNXXXXXXXXXXXXXXXXXXXXXX

3'5' Frame 2FFFFFFYL-PFFFILLSGAPRNFPGGNACSNREHAEDALHRPPLAAEPVLSLWLRLGLFFSLDLTWLRSLSRPRPPRGDLPSCHHHHY-LASRYWCSRSLTSVLSTFTQPSSFMWYILTTPPE-LSL-VA-AIALRPRS-ASSGLRLGR-PLSMTP-A-VFPLPVENIFPESRVPCSST--SPGPFLSQPQIILPIERPMPRYWHIMLDSSLEAADTEIRSPFRR-YSLHSLASRRSQY-QSAAQPDMVPSR-GLISITLFTRKALM-DPAEARESTAAITPCWNLKASVVVPWAISI-TFLSPSPPRDWKNSVGCIPRGKASSGDRIEKL-CPGSERRPLPATGRADSGRCPRGAPHTSSSAMTAQHPEICPLSEEEXXXXXXXXXXXXXXXTXXXIXXXXXXXXXXXXXXXXX

3'5' Frame 3FFFFFFIYNRFSLFYLVGHPETSLGEMLVQIENTQKMHFTGLLWLLSPYSLFGSG-ASSSPWT-RGLGP-VGQDLPEETCPAATTTIIDWLPGTGAAGH-HLYFLLSPNHLPSCGTY-QRLQNSCLCEWHKQ-PCGQGHRPLQG-D-ADSHCP-PRRHKCSHYPWRTYFLRAESHVHPRSRVQDPSYPSHRSYCP-RGPCPGTGTSCWTAAWRLPTLKYVLHFADSTACIPWPAGAPSTDSLLHSQTWCQAGKG-SQSPCSPVRH-CRTQLRPENPLLQSLHAGT-RRAWWCHGPSQSERSFLRPHPGTGRILWAAFPGVKRALEIA-RNCSVLGPSDARFPQPGEPIPAAALGGLRIHLVAP-PPSTQRSVRSRRRNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Blast

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5'3' Frame 1XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSSESGQISGCWAVMALLDVCGAPRGQRPESALPVAGSGRRSDPGHYSFSMRSPELALPRGMQPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ-WWWWQLGRSPLGGLGRLRDLSHVKSKEKKRPSLSQRESTGSAARGGR-SASSACSLFEQAFPPGKFLGAPLSRIKKNGYK-KKKKKK

Blast ( Two or more Sequence )

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Prosite

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