Uct Tipos de Columna

8/12/2019 Uct Tipos de Columna

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Green Earth

page 4

2012

S O L I D P H A S E E X T R A C T I O N

P R O D U C T S C A T A L O GU C T

800.541.0559 www.unitedchem.com

F O R E N S I C S


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F O R E N S I C S C L I N I C A L V E T E R I N A R Y P H A R M A C E U T I C A L

UCT is a respected leader in the drug testing,pharmaceutical, clinical, environmental and

agricultural industries. Our wide range of

highly reproducible solid phase extraction

columns allow the chromatographer a

consistent extraction technique, and our

expertise in silane manufacturing allows a

greater control of the chemical processes

involved in producing our high quality

bonded phase. We manufacture our complete

product line of bonded phases. We manufacture

our complete product line of bonded silicasorbents, packaged in a variety of formats,

including SPE columns, 96 & 48 well plates,

universal cartridges and micro centrifuge tubes.

We also offer a variety of SPE accessories

including derivatizing reagents, GC liners,

and manifolds.

Since our beginnings in 1986, UCT has

evolved into a major competitor in the

field of silica based solid phase extraction

technology. Our commitment to ensuring

the satisfaction of our customers is accom-

plished by delivering on our promises:

top-quality, dependable solid phase

extraction products, and unmatched

technical support.

F O R E N S I C S


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UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226

www.unitedchem.com

CUSTOMER SERVICE

PRICES AND TERMSOur prices are subject to change without notice. The price

in effect when we receive your order will apply. All prices

are in US Dollars and are F.O.B. Lewistown, PA 17044.

Terms of payment are net 30 days.

MINIMUM ORDERS

We welcome all orders, therefore, we do not have a mini-

mum order requirement. When ordering, please include

your purchase order number, complete Ship To and

Bill To address, catalog number, quantity, and descrip-

tion of product(s). Also include your name and a phone

number where you can be reached should we have anyquestions concerning your order.

Custom items will be evaluated on an individual basis; quantity

requirements may be necessary.

SHIPMENTS

Normal processing is within 24-48 hours after receipt

of an order. Unless special shipping requests have

been made, our trained staff will send all orders by

UPS Ground service. The appropriate shipping charges

(freight & insurance costs) will be added to the invoice,

unless otherwise instructed by the customer.

SPECIAL PRICING

We offer special pricing for volume purchases and stand-

ing orders. Please call a sales representative for more

information on special pricing qualifications.

RETURN POLICYOur Quality Manager will handle all returns. Before return-

ing merchandise, please call to obtain a return authorization

number from your sales representative. We will need

to know the reason for the return, date of purchase,

purchase order number and invoice number in order

to issue a return authorization number. Returned mer-

chandise must be received before a credit can be issued.

Returns will not be accepted after 90 days. A restocking

fee of 25% of the price paid, or a minimum of $25.00

(whichever is greater) will be charged on all returns.

WARRANTYAll products manufactured by UCT are guaranteed against

defects in materials and workmanship for a period of 90

days after shipment. UCT will replace any items that

prove to be defective during this time period.

The exclusive remedy requires the end user to first ad-

vise UCT of the defective product by phone or in writing.

Secondly, the defective product must be returned within

30 days after proper approval from our Quality Manager.

All returns must indicate the purchase order number, the

lot number and the shipping date. UCTs total liability is

limited to the replacement cost of UCT products.

This warranty does not apply to damage resulting

from misuse.

Placing An Order

Phone: 215.781.9255 800.541.0559Fax: 215.785.1226

Mail

UCT, Inc.2731 Bartram Rd.Bristol, PA 19007

Technical Support

Phone: 215.781.9255 800.385.3153Fax: 215.785.1226Email: [email protected]: forums.unitedchem.comWeb: www.unitedchem.com


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A G R E E N E R E A R T H

Here at UCT, Inc. we are making an effort to keep the planet cleaner

and greener for everyone. It is our belief that we must act now topreserve our environment for future generations to come.

Organizations we support:

Arbor Day Foundation

Audubon Society

Sierra Club


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TABLE OF CONTENTS

Solid Phase Extraction Phases .....................................................................................................................................................6-8

Reservoirs for Bonded Phase Extractions ......................................................................................................................................9

Use of Bonded Phases for Sample Preparation ...........................................................................................................................10

CLEAN SCREEN Sample Prep Products .................................................................................................................................11-19

Mechanism of CLEAN SCREEN ....................................................................................................................................12

Copolymeric Bonded Phases for Drug Abuse Testing .................................................................................................13

CLEAN SCREEN XCELTM ............................................................................................................................................14-15

CLEAN SCREEN FAStTM ...................................................................................................................................................16

CLEAN SCREENETG / BNZ and CLEAN-THRUTIPS.................................................................................................17

CLEAN SCREEN RSV ................................................................................................................................................18-20

XtrackT HIGH-FLOW Bonded Phases .....................................................................................................................................21-22

CLEAN-UP

Solid Phase Extraction Phases.............................................................................................................................23-46 Hydrophobic Extraction Columns .............. ................ ............... ................ ............... ............... ................ ............... ...24-27

Hydrophilic Extraction Columns .............. ............... ................ ............... ............... ................ ............... ................ .....28-32

Ion Exchange Extraction Columns ................ ............... ................ ............... ................ ............... ............... ................ 33-40

A Map to Optimizing Sample Clean Up using Solid Phase Extraction ..................................................................36-37

Copolymeric Extraction Columns ................ ............... ................ ............... ................ ............... ............... ................ 41-45

Covalent Extraction Columns .............. ............... ............... ................ ............... ................ ............... ................ ............... 46

STYRE SCREEN Polymeric Resin Extraction Columns .........................................................................................................47-48

Benzenesulfonic Acid + C18, Polystyrene Divinylbenzene, Ion Exchange,

Reverse Phase, Carboxylic Acid and Quaternary Amine .............................................................................................48

Method Development Kits ........................................................................................................................................................49-50

Non-Polar Phases Endcapped, Polar Phases, Copolymeric Phases,

Ion Exchange Phases, Toxicology Phases and Pharmaceutical Phases .................................................................... 50

SELECTRASORBTM Bulk Sorbents ............................................................................................................................................51-53

Reservoirs...................................................................................................................................................................................54-55

Frits ..................................................................................................................................................................................................56

Solid Phase Extraction Accessories ..............................................................................................................................................57

Positive Pressure Manifold ......................................................................................................................................................58-59

Universal Vacuum Manifold and Accessories ........................................................................................................................60-61

Vacuum Manifold and Accessories ......................................................................................................................................... 62-63

SELECTRA-SIL Derivatizing Reagents ....................................................................................................................................64-68

GC Liners ....................................................................................................................................................................................69-70

SELECTRA HPLC Columns ...........................................................................................................................................................71

96 Deep Well Plates ...................................................................................................................................................................72-74

48 Deep Well Plates ...................................................................................................................................................................75-77

Flash Chromatography Columns .............................................................................................................................................78-79


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SOLID PHASE EXTRACTION PHASES


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REVERSE PHASE (HYDROPHOBIC)

SORBENT SORBENT CODE STRUCTUREC2 ethyl C02 -SiCH

2CH

3

C3 propyl C03 -Si-(CH2)

2CH

3

C4 n-butyl Cn4 -Si-(CH2)

3CH

3

Ci4 isobutyl Ci4 -Si-CH2CH(CH

3)

2

Ct4 tertiary butyl Ct4 -Si-C(CH3

)3C5 pentyl C05 -Si-(CH

2)

4CH

3

C6 hexyl C06 -Si-(CH2)

5CH

3

C7 heptyl C07 -Si-(CH2)

6CH

3

C8 octyl C08 -Si-(CH2)

7CH

3

C10 decyl C10 -Si-(CH2)

9CH

3

C12 dodecyl C12 -Si-(CH2)

11CH

3

C18 octadecyl C18 -Si-(CH2)

17CH

3

C20 eicosyl C20 -Si-(CH2)

19CH

3

C30 tricontyl C30 -Si-(CH2)

29CH

3

Cyclohexyl CYH1 -SiPhenyl PHY1 -Si

NORMAL PHASE (HYDROPHILIC)

Silica SIL1 -SiOHDiol DOL1 -Si-(CH

2)

3OCH

2CHOHCH

2OH

Cyanopropyl CNP1 -Si-(CH2)

3CN

Florisil FLSAlumina, Acidic ALAAlumina, Neutral ALNAlumina, Basic ALBCarbon CARB

ION EXCHANGE

Anion pkaAminopropyl (1 amine) NAX1 -Si-(CH

2)

3NH

2 9.8

N-2 Aminoethyl (1 & 2 amine) PSA1 -Si-(CH2)

3NH(CH

2)

2NH

2 10.1, 10.9

Diethylamino (3 amine) DAX1 -Si-(CH2)

3N(CH

2CH

3)

2 10.6

Quaternary Amine Chloride QAX1 -Si-(CH2)

3N+(CH

3)

3 Cl- always charged

Quaternary Amine Hydroxide CHQAX1 -Si-(CH2)

3N+(CH

3)

3 OH always charged

Quaternary Amine Acetate CAQAX1 -Si-(CH2)

3N+(CH

3)

3 CH

3COO always charged

Quaternary Amine Formate CFQAX1 -Si-(CH2)

3N+(CH

3)

3 HCOO always charged

Polyimine PAX -Si-(CH2)

3-R-[NHCH

2CH

2]

X

CationCarboxylic Acid CCX1 -Si-CH

2COOH 4.8

Propylsulfonic Acid PCX1 -Si-(CH2)

3SO

3H


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FUNCTIONALIZED SILICA-BASED PHASES

REVERSE PHASE (HYDROPHOBIC)

SORBENT % ORGANIC EXCHANGE (meq/g)C2 ethyl 6.60 N/AC3 propyl 7.60 N/AC4 n-butyl 8.50 N/ACi4 isobutyl 8.80 N/ACt4 tertiary butyl 8.50 N/AC5 pentyl 9.50 N/AC6 hexyl 11.00 N/AC7 heptyl 11.00 N/AC8 octyl 11.10 N/AC10 decyl 15.70 N/AC12 dodecyl not tested N/AC18 octadecyl 21.70 N/AC20 eicosyl 24.30 N/AC30 tricontyl 26.00 N/ACyclohexyl 11.60 N/APhenyl 11.00 N/A

NORMAL PHASE (HYDROPHILIC)

Silica N/A N/ADiol 8.00 N/A

Cyanopropyl 6.90 N/AFlorisil N/A N/AAlumina, Acidic N/A N/AAlumina, Neutral N/A N/AAlumina, Basic N/A N/ACarbon N/A N/A

ION EXCHANGE

AnionAminopropyl (1 amine) 6.65 0.310N-2 Aminoethyl (1 & 2 amine) 9.70 0.320Diethylamino (3 amine) 8.40 0.280Quaternary Amine Chloride 8.40 0.250Quaternary Amine Hydroxide 8.40 0.250

Quaternary Amine Acetate 8.40 0.250Quaternary Amine Formate 8.40 0.250Polyimine 13.5 0.250

CationCarboxylic Acid 9.10 0.170Propylsulfonic Acid 7.10 0.180Benzenesulfonic Acid 11.00 0.320Benzenesulfonic Acid High Load 15.00 0.650Triacetic Acid 7.61 Anion 0.17 / Cation .06

COPOLYMERIC (MULTIFUNCTIONAL PHASES)

Aminopropyl + C8 12.3 0.163Quaternary Amine + C8 13.60 0.160Carboxylic Acid + C8 1 2.50 0.105Propylsulfonic Acid + C8 14.62 0.114Benzenesulfonic Acid + C8 12.30 0.072Cyanopropyl + C8 14.60 0.163Cyclohexyl + C8 N/A N/A

COVALENT PHASES

Epoxy N/A N/AAldehyde N/A N/AIsocyanate 7.1 N/AThiopropyl 6.50 N/A


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Chemistries are offered on these particles sizes...

Small Particle (5-20 m)

Intermediate Particle (25-40 m)

Standard Particle (40-60 m)*

Large Particle (125-210 m)

and are available in the following formats

Stated Volume (mL) Tube Configuration Bed Diameter (mm) Sorbent Mass (mg)

1 Cylindrical 5.5 50-200

3 Cylindrical 8.5 50-1000

6 Cylindrical 12.5 200-200010 Expanded 8.5 50-1000

15 Cylindrical 15.5 500-2000

25 Cylindrical 20 500-5000

75 Cylindrical 27.5 1000-10000

150 Cylindrical 38.0 10000-70000

RESERVOIRS FOR BONDED PHASE EXTRACTIONS


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USE OF BONDED PHASES FOR SAMPLE P REPARATION

CONDITIONING, SOLVATION (WETTING)

Columns are shipped dry, but those with hydrophobiccharacter need to be solvated in order to interact efficientlyand reproducibly with aqueous matrices. Sample capacity isseverely reduced on a dry column.

At low vacuum ( - 3 in. Hg) add 1.5 mL of methanol or aceto-nitrile per 100 mg of sorbent to the sample preparation col-umn. Release the vacuum or begin flushing immediately uponcompletion. The more air which passes through the columnbefore sample loading, the less solvated the sorbent will be.

Apply deionized or distilled water to remove excess solventwhich will interfere with hydrophobic binding. Use 1 mL H2Oper 100 mg sorbent. Momentary high vacuum (5 to 8 in. Hg)may be necessary to restart flow. At - 2.5 in. Hg the column

will resist air displacement (vacuum may be left on withoutdrying the sorbent). If the sorbent is accidentally dried, resol-vate and reflush.

When using ion exchange columns, apply 1mL of buffer tothe column after flushing to ensure that the sorbent pH isoptimal for the sorbent analyte interaction desired. Whereion exchange interactions are involved, follow guidelinesconcerning pKa, pH and ionic binding. Use the same vacuumguidelines as described for flushing.

When doing work where a very clean baseline is requiredwashing the column with the elution solvent as part of theconditioning step will improve the LOD and LOQ.

SAMPLE PREPARATION AND APPLICATION

Mechanisms may be hydrophobic, polar, or ionic. Add internalstandard to the sample if quantitation is desired. Optimizesample application by removing particulates if necessary(centrifugation or filtering) and/or diluting viscous matriceswith water or buffer to ensure proper pH for desired inter-actions. The analyte and sorbent should be uncharged foroptimum hydrophobic retention. On ion exchange sorbents,analytes must be oppositely charged to the sorbent [anions(-) on anion exchange sorbents (+); cations (+) on cation ex-change sorbents (-)]. During sample application, the analytebinds by displacing a counterion on the sorbent.

Apply sample at a rate of 1mL/min. Again, a momentary

increase in vacuum may be needed to initiatesample flow.

WASHING THE SORBENT AND ELUTING

Ideal washing removes as many interferences as possiblewhile retaining the analyte(s). Ideal elution recovers 100% ofthe analyte while leaving behind interferences. Make certainyour column is dry when changing between aqueous solu-tions and organic solvents.

HYDROPHOBIC AND POLAR ANALYTES

The best approach towards using these types of sorbents isto search for a solvent mixture which will wash the most inter-ferences from the sorbent without loss of analyte. Note thatwash pH may greatly affect cleanup and/or recovery. Keepanalyte and sorbent pKa in mind if applicable. Elute with thestrongest organic solvent, or by raising the percentage oforganic, possibly in combination with a pH change to disrupt

binding.

ION EXCHANGE

Ionic bonds are strong enough to allow the analyte to remainbound while interferences are washed away with high per-centages (up to 100%) of polar or nonpolar organic solvents.The pH will also affect sample cleanup. Remember to remain2 pH units from the relevant pKa of your analyte and sorbent,both of which need to remain charged for ionic retention.Elute with aqueous buffers containing a stronger counterionthan your analyte (classic ion exchange) or by changing pH todisrupt the ionic attraction. Make sure the elution solvent hasenough organic character to overcome any adsorption due to

the packing material.

COPOLYMERIC EXCHANGE

For ionically bound analytes, use washes of high organicstrength to remove interferences retained by hydrophobic(solvent strength dependent) interactions. If your analyte isalso capable of hydrophobic binding, remove polar interfer-ences ionically similar to your analyte by using aqueous orweak aqueous/organic washes while disrupting ionic (pH andionic strength dependent) binding. Elute by simultaneouslydisrupting ionic and hydrophobic interactions


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CLEAN SCREEN SAMPLE PREP PRODUCTS


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MECHANISM OF CLEAN SCREEN

Sample ApplicationWhen a sample is loaded onto the column at pH 6, many carboxylicacid functionalities present in the sample are ionized. This creates arepulsion between the column and many sample borne interferences,thereby reducing the likelihood of their adsorbing onto the column.At this pH, ibuprofen & barbiturates are not i onized and arehydrophobically adsorbed on to the column (figure 1). At the sametime, drugs with amine functionalities such as cocaine andphencyclidine adsorb on to the column via both hydrophobic andionic attraction (figure 1).

The column can then be washed with water or weak aqueousbuffers at or below pH 6 without risking loss of the analytes. Afterdrying the column, it is possible to elute the hydrophobically boundanalytes using solvents of minimal polarity such as methylenechloride or a hexane/ethyl acetate mixture (figure 2). Cationic

analytes will remain bound to the column. Many compounds ofintermediate polarity and potential interferences will also remainon the column. The majority of these potential interferences canbe removed by using a methanol wash.

Cationic analytes bound to the column can be eluted after anotherdrying step. The drying steps are necessary to remove water whichwould have prevented the water immiscible elution solvents fromoptimally interacting with the analytes (figure 3).

To elute the cationic analytes, an organic solution with a high pHshould be used. A methylene chlorideisopropanol-ammoniumhydroxide mixture will simultaneously disrupt these ionic

interactions and successfully elute the desired compound (figure 4).

NH+

PCP

SO3

CnH2n+1

NH+

PCPSO3

CnH2n+1

H2O

NH+

PCP

IBUPROFEN

SO3

CnH2n+1

H

CH3 O

figure

Dry Column

Elution 2

NH+

PCP

IBUPROFEN

SO3

CnH2n+1

CH C OH

CH3 O

Elution 1

figure 2

figure 3

figure 4

CH C O


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CLEAN SCREEN

COPOLYMERIC BONDED PHASES FOR DRUG ABUSE TESTIN

Analytical demand for more efficient, robust and clean extraction of drugs from biological matrices led to thedevelopment of CLEAN SCREENsorbents. Since 1986, CLEAN SCREENhas led the industry with dependableand reproducible Solid Phase Extraction products and applications. CLEAN SCREENphases are true copolymericsorbents that contain hydrophobic and ion exchange functional groups uniquely polymerized to a silica substrate.The design and quality of CLEAN SCREENprovides superior sample clean up, recovery and reproducibility.

Mixed mode separations allow maximum selectivity for extraction of acids, neutrals and bases. This selectivitymakes CLEAN SCREENideal for both screening and confirmation analysis for virtually all drug categories.CLEAN SCREENcolumns are used extensively by forensic and clinical chemists including:

Note:If performing extractions out of viscous matrices such as tissue or horse urine, turn to our XtrackT section,where high-flow/gravity flow columns are found. The DAU CLEAN SCREEN sorbent as well as other phasesare available in this larger particle size.

Post Mortem Investigations Criminal Investigations Urine Drug Testing Athletic Drug Testing

Racing Laboratories Therapeutic Drug Monitoring Medical Drug Screening

CLEAN SCREENDAU

column is copolymerized on arigid, purified silica gel support.The two functional groupsinclude a reverse phase, and anion exchanger, benzenesulfonicacid. This column is commonlyused for analyzing a wide rangeof drugs of abuse, includingacidic, basic & neutral drugs.

Application:Dual functionalityfor weak bases and hydrophobiccompounds.

CLEAN SCREENTHC

column is copolymerized on arigid, purified silica gel support.The two functional groups includea reverse phase, and an ionexchanger, primary amine.This column is commonlyusedfor analyzing THC andits metabolites.

Application:Dual functionality for

acids and hydrophobic compounds.

DAU

Part Number without Part Number with Sorbent Amount/ Unit per

Clean-ThruTips Clean-ThruTips Tube Volume Pack

CSDAU131 CCDAU131 130mg/1mL 100CSDAU133 CCDAU133 130mg/3mL 50CSDAU203 CCDAU203 200mg/3mL 50CSDAU303 CCDAU303 300mg/3mL 50CSDAU503 CCDAU503 500mg/3mL 50CSDAU206 CCDAU206 200mg/6mL 50CSDAU506 CCDAU506 500mg/6mL 50CSDAU1M6 CCDAU1M6 1g/6mL 30ZSDAU005 ZCDAU005 50mg/10mL 50ZSDAU013 ZCDAU013 130mg/10mL 50ZSDAU020 ZCDAU020 200mg/10mL 50CSDAU515 CCDAU515 500mg/15mL 5

% Organic Loading: 12.30 Exchange Capacity (meq/g): 0.072

THC



CSTHC131 CCTHC131 130mg/1mL 100CSTHC203 CCTHC203 200mg/3mL 50CSTHC303 CCTHC303 300mg/3mL 50CSTHC503 CCTHC503 500mg/3mL 50CSTHC206 CCTHC206 200mg/6mL 50CSTHC506 CCTHC506 500mg/6mL 50CSTHC1M6 CCTHC1M6 1g/6mL 30ZSTHC013 ZCTHC013 130mg/10mL 50ZSTHC020 ZCTHC020 200mg/10mL 50

% Organic Loading:12.3 Exchange Capacity (meq/g):0.163

GHB

Part Number without Part Number with Unit per

Clean-ThruTips Clean-ThruTips Pack

CSGHB203 200mg/3mL 50ZSGHB020 200mg/10mL 50

The small polar nature of the molecule and the lack of a UV chromatophore complicate the chromatographic andspectrophotometricanalysis of GHB. Chemically, GHB is unstable and readily forms Gamma-butyrolactone when heated inacid conditions. Most analytical methods are based upon the interconversion to the lactone and chemical derivatization to

form the TMS derivative. This column is for the extraction of free GHB.


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CLEAN SCREEN XCELTM 2 STEP EXTRACTION COLUMN


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CLEAN SCREEN XCEL

SOLID PHASE EXTRACTION COLUMNS

CLEAN SCREEN XCEL I

Part Number Sorbent Amount/ Unit per

Endcapped Tube Volume Pack

CSXCE111 130mg/1mL 100CSXCE103 130mg/3mL 50CSXCE106 130mg/6mL 50ZSXCE010 130mg/10mL 50

CLEAN SCREEN XCEL II



CSXCE211 130mg/1mL 100CSXCE2103 130mg/3mL 50CSXCE2106 130mg/6mL 50ZSXCE2010 130mg/10mL 50

CLEAN SCREEN XCEL

SOLID PHASE EXTRACTION WELL PLATES

96 DEEP WELL PLATES


Tube Volume Pack

WSH96XCE11 130mg 1

48 DEEP WELL PLATES

Part Number Sorbent Amount/ Unit per Tube Volume Pack

WSH48XCE11 130mg 1

CLEAN SCREEN XCEL I:

The Xcel I column will extract awide array of basic drugs includingbenzodiazepines and opiates.

CLEAN SCREEN XCEL II:

The Xcel II column is designed solely forthe extraction of the THC metabolite.

The Clean Screen Xcel solid phase extraction columns are a line of sample prep phases designedto reduce the number of steps in the extraction, and therefore the amount of time and solventnecessary to complete the sample cleanup. Further advantages include reduced sample size andimproved cleanliness and recovery.


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CLEAN SCREEN FASt

SOLID PHASE EXTRACTION COLUMNS

CLEAN SCREEN FAStTM



CSFAS203 200mg/3mL 50

ZSFAS020 200mg/10mL 50

Benefits: Eliminate the centrifuge and sample transfer steps Lower costs by decreasing turn-around time Reducing instrument and LC column maintenance

CLEAN SCREEN FAStTMemploys a process that uses positive pressure and a solid phase sorbent bed builtwith small pore frits to quickly and efficiently prepare samples for LC/MS analysis. This method eliminatesthe timely centrifugation, reduces matrix suppression effects and removes particulates greater than ~ 1m.Samples can be diluted at a ratio as low as 1:1, which is `useful for analytes at very low concentrations.

A FASTER AND CLEANER SPE

ALTERNATIVE TO

DILUTE AND SHOOT


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CLEAN-THRU TIPS

CLEAN SCREEN

ETHYL GLUCURONIDE SOLID PHASE EXTRACTION COLUMN

BENZODIAZEPINE SOLID PHASE EXTRACTION COLUMN

ETG


Clean-Thru

Tips Clean-Thru

Tips Tube Volume PackCSETG203 CCETG203 200mg/3mL 50ZSETG040 ZCETG040 400mg/10mL 50

BNZ



CSBNZ203 CCBNZ203 200mg/3mL 50CSBNZ206 _______ 200mg/6mL 50ZSBNZ020 _______ 200mg/10mL 50ZSBNZ030 _______ 300mg/10mL 50

CLEAN-THRUTIPS

Part Number Description Unit per bag

CLTTP050 TIP 50

CLEAN SCREENETG

columns are available exclusivelyfrom UCT for analysis by GC/MS &LC/MS. This 3mL column contains aproprietary carbon packingmaterial for the extraction andconcentration of ethyl glucuronide.

CLEAN SCREENBNZ

columns are available forbenzodiazepine extractions,with specific focus on7-amino benzodiazepines.

CLEAN-THRU tips provides the first solid phase extraction cartridge system that eliminates sample carry overfrom the vacuum manifold lid. The technology was pioneered by our research scientists & consists of one of ourSPE columns with a disposable tip that attaches to the end of each column. Columns available in the CLEAN-THRU configuration are found in the CLEAN SCREENsections. This system was designed in order to meet the strictrequirements that the Substance Abuse and Mental Health Services Administration certification has placed on labo-ratories to address the problem of cross contamination between samples. CLEANTHRUtips provide a completely

disposable system that eliminates any contact between the sample, wash solvents and the extraction apparatus.The continuous passage of the sample through the system provides a direct, accurate route to waste or collectionvessels. As each extraction is completed, the column and tip are discarded as a unit. CLEAN-THRUeliminates yourconcerns about sample residue remaining in the extraction system.U CLEAN SCREENsorbent as well as otherphases are available in this larger particle size.

StandardConfiguration

UCTs Column with

a CLEAN-THRUTip


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Advantages of CLEAN SCREENRSV:

75% Reduction in total liquid volumes

Lower cost per extraction

Faster extraction times

Less disposal cost

Increased automated throughput

50% Reduction in eluate volume

Faster dry down times

Reduced exposure to organic solvents

Superior flow characteristics

Less flow restriction from matrix proteins

or particulates

More reliable for automated processing

High capacity

Greater linear range

CLEAN SCREENRSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS

Reduced Solvent Volume extraction columns are micro bed packed columns whichoffer the advantages of disc technology yet maintain the proven track record of ourconventional SPE columns. Reduced Solvent Volume columns use 75% less solvent thantraditional packed columns. Less solvent means faster extractions, higher throughputand less waste disposal, which translates into significant savings in both time andmoney. Results demonstrate that therapeutic and abused drugs in urine and bloodmatrices can be extracted with cleanliness, high recoveries and consistent reproducibilityby using the Reduced Solvent Volume extraction column.


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CAPACITY

SOLVENT REDUCTION

RECOVERY

CLEAN SCREEN RSV

REDUCED SOLVENT VOLUME EXTRACTION COLUMNS

91% (n=4) for 1000 ng/mL90% (n=2) for 3000 ng/mL

91% (n=4) for 4000 ng/mL

No analyte breakthrough at 1000 ng/mL

and less than 0.2% analyte breakthrough

at 3000 and 4000 ng/mL levels.

Linear Ranges Exhibiting

Greater than

85% Recoveries in Urine

THC-COOH Standards (ng/mL) in spiked urine

Analyte

AbsoluteRecovery

Concentration(ng/mL)

100908070605040302010

0

200018001600140012001000

800600400200

0

1000 3000 4000

Highest Concentration Tested

Lowest Concentration Tested

Analyte Solvent Usage Solvent Usage % Solvent

Clean-ThruTips Reduced Solvent Traditional Packed Reduction

Volume SPE columns columns

Barbiturates 4.25 mL 18 mL 76%Benzoylecgonine 4.65 mL 19 mL 76%THC-COOH 4.85 mL 16.4 mL 73%Phencyclidine 5.15 mL 19 mL 70%

Butalbital

Amobarbital

Pentobarbital

Secobarbital

Phenobarbital

Benzoylecgonine

Phencyclidine

THC-COO

H

2ng/mL25ng/mL


20/80U C T


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CLEAN UP RSV


CLEAN SCREEN RSV


COPOLYMERIC EXTRACTION FOR ALL DRUG CLASSES



CSDAUA83 CCDAUA83 80mg/3mL 50ZSDAUA08 ZCDAUA08 80mg/10mL 50

SPECIFICALLY FOR THC & THCA EXTRACTION



CSTHCA83 CCTHCA83 80mg/3mL 50ZSTHCA08 ZCTHCA08 80mg/10mL 50

C18, OCTADECYL TRI-FUNCTIONAL

Part Number Part Number Sorbent Amount/ Unit per Pack

Endcapped Unendcapped Tube Volume Pack

CEC18A083 CUC18A083 80mg/3mL 50

CLEAN SCREENDAU

Column is copolymerized on a rigid,

purified silica gel support. The twofunctional groups include a reverse phaseand an ion exchanger, benzenesulfonicacid. This column is commonly used foranalyzing a wide range of drugs of abuse,including acidic, basic and neutral drugs.

CLEAN SCREENTHC

Column is copolymerized on a rigid,purified silica gel support. The twofunctional groups include a reversephase, and an ion exchanger, quaternary

amine. This column is commonly usedfor analyzing THC and its metabolites.

% Organic Loading:21.70Application:

Removes hydrophobic impurities,de-salting and purificationof hydrophobic compounds.


21/80U C


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XtrackT HIGH-FLOW SOLID PHASE EXTRACTION COLUMNS


22/80U C T


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XtrackT

HIGH-FLOW BONDED PHASES

Viscous sample matrices are frequently resistant to flow through standard solid phase columns. Increased particlesize enhances flow characteristics allowing ease of sample application and analysis. XtrackTcolumns are designedto give uniform flow for even the most viscous samples including equine urine, post mortem blood and tissues,meconium, amniotic fluid, milk, etc.

XtrackTalso functions as a gravity flow column for most blood and urine samples. A single column providesextraction for a broad spectrum of compounds with selective elution of acid neutrals, steroids and bases XtrackTyields very clean extractions and excellent recoveries without need for additional liquid clean up steps. XtrackT is available in hydrophobic, hydrophilic, ion exchange, and copolymeric phases, including the CLEAN SCREENDAU sorbent. XtrackTis recommended for any chemist challenged by viscous sample matrices, or those desiringgravity flow capacity.

Upon request, we can also provide any of our CLEAN-UPsorbents in this large particle size.

Advantages of XtrackT

Resists plugging Improved flow of all sample types Gravity flow of most samples

Broad spectrum extractions Very clean extractions High recoveries Reproducibility

GRAVITY-FLOW CLEAN SCREENDAU COLUMNS

Part Number with Part Number without Sorbent Amount/ Unit per


XRDAH203 XCDAH203 200mg/3mL 50XRDAH303 XCDAH303 300mg/3mL 50XRDAH503 XCDAH503 500mg/3mL 50XRDAH206 XCDAH206 200g/6mL 50XRDAH506 XCDAH506 500g/6mL 50XRDAH20Z XCDAH20Z 200mg/10mL 50XRDAH50Z XCDAH50Z 500mg/10mL 50XRDAH515 XCDAH515 500mg/15mL 50XRDAHM15 XCDAHM15 1g/15mL 30

CLEAN SCREENDAU

Column is copolymerized on a rigid,purified silica gel support. The twofunctional groups include a reversephase, and an ion exchanger ,benzenesulfonic acid. This column iscommonly used for analyzing a widerange of drugs of abuse, includingacidic, basic & neutral drugs.

HIGH-FLOW CLEAN-UPENDCAPPED C18 COLUMNS

Part Number with Part Number without Sorbent Amount/ Unit

per


XRODHM06 XCODHM06 1g/6mL 30XRODH515 XCODH515 500mg/15mL 50

% Organic Loading: 21.70

Application:

Removes hydrophobic impurities,de-salting and purification ofhydrophobic compounds.

HIGH-FLOW CLEAN-UPPROPYLSULFONIC ACID COLUMNS

Part Number with Part Number without Sorbent Amount/ Unit perClean-ThruTips Clean-ThruTips Tube Volume Pack

XRPCH50Z XCPCH50Z 500mg/10mL 50


Application:

Scavenger for amines,alcohols and other nucleophiles

High-Flow also available in:

Carboxylic Acid, Aminoethyl, Benzenesulfonic Acid and Quaternary Amine


23/80U C


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HYDROPHOBIC HYDROPHILIC ION EXCHANGE

CLEAN-UP SOLID PHASE EXTRACTION COLUMNS


24/80U C T


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CLEAN-UP

HYDROPHOBIC EXTRACTION COLUMNS

This sorbent is composed of a silica backbone bonded

with hydrocarbon chains. It is used to extract compounds

which exhibit non-polar or neutral characteristics out of

complex matrices. The C18 phase is the most widely used

for non-polar interactions because of its nonselective nature;

C18 will extract a large number of compounds with differing

chemical properties. To enchance selectivity, UCT offers a

wide range of hydrophobic sorbents. Multiple chain configu-

rations are available for some Endcapped or unendcapped

sorbents are available for all chain lengths.

Analytes* Washes Elutions

alkanes aqueous, non-polar

alkenes usually with to

aromatics some polar polar organic

neutral compounds organic solvent

*typical compounds which can be extracted using hydrophobic columns

Silica Backbone

Hydrocarbon Chain

O

Si

O

Si

O

Si

O

Si

O Si (CH2)17 CH3

CH3(CH2)SiO

OSi(CH3

)3

OSi(CH3)3

OSi(CH3)3

OSi(CH3)3

17

Example of a Hydrophobic Phase


25/80U C


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CLEAN-UP


MECHANISM OF HYDROPHOBIC BONDING

Compounds are retained by non-polar interactionsfrom polar solvents or matrix environments. They are

bound by dispersion forces / van der Waals forces.Elution, or disruption of the non-polar interactionsis achieved by solvents or solvent mixtures with suf-ficient non-polar character. Some polar solvents, suchas acetonitrile have enough non-polar characteristicsto disrupt nonpolar binding to cause elution of acompound from the sorbent. Methanol can be usedas well, although it should be noted that it will takeoff both polar and nonpolar analytes of interest andinterferences.

UNENDCAPPED VS. ENDCAPPED

Bonded phases are manufactured by the reactionof organosilanes with activated silica. During thepolymerization reaction of carbon chains to the silicabackbone, a very stable silyl ether linkage forms. Ourunendcapped columns allow hydroxyl sites to remain,thus making these columns slightly hydrophilic.

In order to decrease this slight polarity, thesehydroxyl sites are deactivated. Proprietary bondingtechniques ensure that these sites are 100% reacted,leading to a complete endcapping. Because thereare no hydroxyl sites left, our endcapped columnsare more hydrophobic than our unendcapped columns.

Sorbent

Compound

H

C

H

H

C

H

H

C

H

H

C

H

H

C

H

H

C

H

O

O

O

SiSi

Si

OSi(CH3)3

OSi(CH3)3

O

O

O

SiSi

Si

OH

OH

Unendcapped Structure Endcapped Structure

Example of a Hydrophobic Bonding

Sorbent Structure

C2 ethyl -SiCH2CH3

C3 propyl -Si-(CH2)

2CH

3

C4 n-butyl -Si-(CH2)

3CH

3

Ci4 isobutyl -Si-CH2CH(CH

3)

2

Ct4 tertiary butyl -Si-C(CH3)3

C5 pentyl -Si-(CH2)

4CH

3

C6 hexyl -Si-(CH2)

5CH

3

C7 heptyl -Si-(CH2)

6CH

3

C8 octyl -Si-(CH2)

7CH

3

C10 decyl -Si-(CH2)

9CH

3

C12 dodecyl -Si-(CH2)

11CH

3

C18 octadecyl -Si-(CH2)

17CH

3

C20 eicosyl -Si-(CH2)

19CH

3

C30 tricontyl -Si-(CH2)

29CH

3

Cyclohexyl -Si

Phenyl -Si

Hydrophobic Sorbents & Structures


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CLEAN-UP


C8, OCTYL

Part Number Part Number Sorbent Amount/ Unit per


CEC081L1 CUC081L1 50mg/1mL 100CEC08111 CUC08111 100mg/1mL 100CEC08113 CUC08113 100mg/3mL 50CEC08123 CUC08123 200mg/3mL 50CEC08153 CUC08153 500mg/3mL 50CEC08156 CUC08156 500mg/6mL 50CEC081M6 CUC081M6 1g/6mL 30CEC0811Z CUC0811Z 100mg/10mL 50CEC0812Z CUC0812Z 200mg/10mL 50CEC0812M15 CUC0812M15 2g/15mL 20CEC0815M25 CUC0815M25 5g/25mL 20

C18, OCTADECYL



CEC181L1 CUC181L1 50mg/1ml 100CEC18111 CUC18111 100mg/1ml 100CEC18113 CUC18113 100mg/3ml 50CEC18123 CUC18123 200mg/3ml 50CEC18153 CUC18153 500mg/3ml 50CEC18156 CUC18156 500mg/6ml 50CEC181M6 CUC181M6 1g/6ml 30CEC1811Z CUC1811Z 100mg/10ml 50CEC1812Z CUC1812Z 200mg/10ml 50CEC1815Z CUC1815Z 500mg/10ml 50CEC1812M15 CUC1812M15 2g/15ml 20CEC1815M25 CUC1815M25 5g/25ml 20

CEC18110M375 CUC18110M75 10g/75ml 10

C30, TRICONTYL



CEC30111 CUC30111 100mg/1mL 100CEC30123 CUC30123 200mg/3mL 50CEC30156 CUC30156 500mg/6mL 50CEC301M6 CUC301M6 1g/6mL 30


Application:

Removes large or morehydrophobic compounds.


Application:



Application:



27/80U C


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CLEAN-UP


CYCLOHEXYL



CECYH123 CUCYH123 200mg/3mL 50CECYH153 CUCYH153 500mg/3mL 50CECYH1M6 CUCYH1M6 1g/6mL 30CECYH12M15 CUCYH12M15 2g/15mL 20

PHENYL



CEPHY1L1 CUPHY1L1 50mg/1mL 100

CEPHY111 CUPHY111 100mg/1mL 100CEPHY123 CUPHY123 200mg/3mL 50CEPHY153 CUPHY153 500mg/3mL 50CEPHY156 CUPHY156 500mg/6mL 50CEPHY1M6 CUPHY1M6 1g/6mL 30CEPHY12Z CUPHY12Z 200mg/10mL 50


Application:

Scavenger for phenolic compounds.


Application:

Scavenger for polar compounds.

OTHER REVERSE PHASES AVAILABLE:

C2 ethyl

C3 propyl

C4 n-butyl

Ci4 isobutyl

Ct4 tertiary butyl

C5 pentyl

C6 hexyl

C7 heptyl

C10 decyl

C12 dodecyl

C20 eicosyl


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This sorbent is composed of a

silica backbone bonded with

carbon chains containing polar

functional groups. Groups which

will possess such polarity include

amines, hydroxyls and carbonyls.

Analytes* Washes Elutions

R-OH, R-SH non-polar organic polar organic

R-NH2, solvents solvent

R2-NH, ie: hexane/ethyl acetate (80:20) usually with

R3-N methylene chloride some aqueous

CLEAN-UP HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS

O

Si

O

Si

OSi

O

Si

O Si (CH2)3 CH2O CH 2CH

OH OH OH

OH

O Si (CH2)3 CH2O CH 2CH

OH OH OH

OH

Silica BackboneHydrophilic Chain

Example of a Hydrophilic Phase


29/80U C


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Mechanism of

Hydrophilic Bonding

Compounds are retained on hydrophilic

sorbents through polar interactions including

hydrogen bonding, pi-pi or dipole-dipole

interaction. These types of interactions

occur when a distribution of electrons

between individual atoms in functional

groups is unequal, causing negative and

positive polarity. Compounds typically

extracted on a hydrophilic column include

analytes which have polar groups,

including amines, hydroxyls and carbonyls.

Elution is performed by strong polar solvents.

Sorbent Structure

Silica -SiOH

Diol -Si-(CH2)

3OCH

3CHOHCH

2OH

Cyanopropyl -Si-(CH2)

3CN

Note: If un-ionized, ion exchange sorbents can be used as hydrophilic (polar) sorbents.

Hydrophilic

Sorbents & Structures

Example of Hydrophilic Bonding

HO

CH2

OH

CH

HO C CH2

O

CH2Compound

Sorbent

+

+

-

-

CLEAN-UP HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS


30/80U C T


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CLEAN-UP

HYDROPHILIC EXTRACTION COLUMNS

UNBONDED SILICA (ACID WASHED)



CUSIL111 100mg/1mL 100CUSIL123 200mg/3mL 50CUSIL153 500mg/3mL 50CUSIL156 500mg/6mL 50CUSIL1M6 1g/6mL 30CUSIL11Z 100mg/10mL 50CUSIL12M15 2g/15mL 20CUSIL15M25 5g/25mL 20CUSIL110M75 10g/75mL 10

PHARMA-SIL


Endcapped Tube Volume PackPHSIL1L1 50mg/1mL 100PHSIL123 200mg/3mL 50PHSIL156 500mg/6mL 50PHSIL1M6 1g/6mL 30PHSIL12M15 2g/15mL 20PHSIL15M25 5g/25mL 20

HIGH SURFACE ACTIVITY SILICA



HSSIL153 500mg/3mL 50HSSIL156 500mg/6mL 50

FLORISIL



CUFLS111 100mg/1mL 100CUFLS123 200mg/3mL 50CUFLS153 500mg/3mL 50

CUFLS156 500mg/6mL 50CUFLS1M6 1g/6mL 30CUFLS11Z 100mg/10mL 50CUFLS12Z 200mg/10mL 50CUFLS15Z 500mg/10mL 50CUFLS12M15 2g/15mL 20CUFLS15M25 5g/25mL 20CUFLS110M75 10g/75mL 10

40-63 m Particle Size60A Pore Size

500m2/g Surface Area

Application:

Removes hydrophilic (polar)impurities, purification ofhydrophilic (polar) compounds.

40-63 m Particle Size

60A Pore Size525m2/g Surface Area

Application:


40-63 m Particle Size60A Pore Size547m2/g Surface Area

Application:


% Organic Loading: N/A

Application:

Removes polar-type compounds.

Florisil

products aremanufactured byU.S. Silica, Co.


31/80U C


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CLEAN-UP


ALUMINA, ACIDIC



CUALA111 100mg/1mL 100CUALA123 200mg/3mL 50CUALA153 500mg/3mL 50CUALA156 500mg/6mL 50CUALA1M6 1g/6mL 30CUALA12M15 2g/15mL 20

ALUMINA, BASICPart Number Sorbent Amount/ Unit per


CUALB123 200mg/3mL 50CUALB153 500mg/3mL 50CUALB156 500mg/6mL 50CUALB1M6 1g/6mL 30CUALB12Z 200mg/10mL 50CUALB15Z 500mg/10mL 50CUALB12M15 2g/15mL 20CUALB15M25 5g/25mL 20CUALB110M75 10g/75mL 10

ALUMINA, NEUTRAL



CUALN1L1 50mg/1mL 100CUALN111 100mg/1mL 100CUALN123 200mg/3mL 50CUALN153 500mg/3mL 50CUALN156 500mg/6mL 50CUALN1M6 1g/6mL 30CUALN12Z 200mg/10mL 50CUALN15Z 500mg/10mL 50CUALN12M15 2g/15mL 20

CUALN15M25 5g/25mL 20


Application:Removes polar-type compounds.


Application:



Application:



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Application:Removes steroid-type compounds.


Application:


CLEAN-UP


CN, CYANOPROPYL



CECNP1L1 CUCNP1L1 50mg/1mL 100CECNP111 CUCNP111 100mg/1mL 100CECNP123 CUCNP123 200mg/3mL 50CECNP153 CUCNP153 500mg/3mL 50CECNP156 CUCNP156 500mg/6mL 50CECNP1M6 CUCNP1M6 1g/6mL 30CECNP12M15 CUCNP12M15 2g/15mL 20CECNP110M75 CUCNP110M75 10g/75mL 10

DIOL



CUDOL111 100mg/1mL 100CUDOL123 200mg/3mL 50CUDOL153 500mg/3mL 50CUDOL156 500mg/6mL 50CUDOL15Z 500mg/10mL 50CUDOL12M15 2g/15mL 20

CARBON-GRAPHITIZED NON-POROUS, 120/400 MESH



CUCARB1L1 50mg/1mL 100CUCARB111 100mg/1mL 100CUCARB123 200mg/3mL 50CUCARB153 500mg/3mL 50CUCARB156 500mg/6mL 50CUCARB1M6 1g/6mL 30

CLEAN-UP Carbon

Application:

Carbon supports have been used toisolate extremely polar organic com-pounds. They work by a hydrophobicmechanism with a high surface areaand ion exchange. This interaction canhappen in a wide range of polar andnon-polar solvents.


33/80U C


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UThis sorbent is composed of a silica back-bone bonded with a carbon chain terminatedby a negatively or positively charged functionalgroup. Ion exchange interactions occur be-tween a sorbent that carries a charge anda compound of opposite charge.

This electrostatic interaction is reversibleby neutralizing the sorbent and /or analyte.Ion exchange bonds can also be disruptedby introduction of a counter ion to com-pete with the analyte for binding sites onthe sorbent.

Anions Cations

Organic solvent or aqueous

buffer at pH that allows the ion

to remain charged AND/OR at

a low ionic strength AND/OR

at a weak concentration.

Organic solvent or aqueous

buffer at pH that would neutralize

the ion AND/OR at a high

ionic strength AND/OR at a

strong concentration.

Si

O

Si

O

Si

O Si (CH2)2

OH

OH

SO3

SO3

O

Si

O

O Si (CH2)

OH

2

OH

Silica Backbone

Anion Exchanger

Example of a Cation Exchange Phase

Example of a Anion Exchange Phase

O

Si

O

Si

O

Si

O

Si

O Si (CH2)3 NH+

3

NH +3(CH2)3SiO

OH

OH

OH

OH

Silica Backbone

Anion Exchanger

CLEAN-UP ION EXCHANGE EXTRACTION COLUMNS

)


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MECHANISM OF ION EXCHANGE BONDING

Compounds are retained on the sorbent through ionic bonds. Therefore, it is essential that the sorbent and theanalyte to be extracted are charged. Generally, the number of molecules with charged cationic groups increasesat pH values below the molecules pKa value. The number of molecules with charged anionic groups decreases atpH values below the molecules pKa value. To ensure 99% or more ionization, the pH should be at least two pHunits below the pKa of the cation and two pH units above the pKa of the anion. Elution occurs by using a solventto raise the pH above the pKa of the cationic group or to lower the pH below the pKa of the anion to disruptretention. At this point, the sorbent or compound will be neutralized.

ION EXCHANGE SORBENTS & STRUCTURE

Sorbent Structure pKa

Anion Exchangers

Aminopropyl (1 amine) -Si-(CH2)3NH

3+ 9.8

N-2 Aminoethyl (1 & 2 amine) -Si-(CH2)3NH

2+(CH

2)2NH

3+ 10.1, 10.9

Diethylamino (3 amine) -Si-(CH2)3NH+(CH

2CH

3)2 10.6

Quaternary Amine Chloride -Si-(CH2)3N+(CH

3)

3 Cl- always charged

Quaternary Amine Hydroxide -Si-(CH2)3N+(CH

3)

3 OH always charged

Quaternary Amine Acetate -Si-(CH2)3N+(CH

3)

3 CH

3COO always charged

Quaternary Amine Formate -Si-(CH2

)3

N+(CH3

)3

HCOO always chargedPolyimine -Si-(CH

2)3R-[NHCH

2CH

2]

X

Cation Exchangers

Carboxylic Acid -Si-CH2COOH 4.8

Propylsulfonic Acid -Si-(CH2)3SO

3H


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CLEAN-UP

ANION EXTRACTION COLUMNS

AMINOPROPYL


Tube Volume Pack

CUNAX1L1 50mg/1mL 100CUNAX111 100mg/1mL 100CUNAX123 200mg/3mL 50CUNAX153 500mg/3mL 50CUNAX156 500mg/6mL 50CUNAX1M6 1g/6mL 30CUNAX11Z 100mg/10mL 50CUNAX12Z 200mg/10mL 50CUNAX15Z 500mg/10mL 50CUNAX12M15 2g/15mL 20CUNAX15M25 5g/25mL 20CUNAX110M75 10g/75mL 10

PSA ( N-2 AMINOETHYL )


Tube Volume Pack

CUPSA1L1 50mg/1mL 100CUPSA111 100mg/1mL 100CUPSA123 200mg/3mL 50CUPSA153 500mg/3mL 50CUPSA156 500mg/6mL 50CUPSA1M6 1g/6mL 30CUPSA11Z 100mg/10mL 50CUPSA110M75 10g/75mL 10

DIETHYLAMINO


Tube Volume Pack

CUDAX111 100mg/1mL 100CUDAX123 200mg/3mL 50CUDAX153 500mg/3mL 50CUDAX156 500mg/6mL 50CUDAX12M15 2g/15mL 20CUDAX15M25 5g/25mL 20


Exchange Capacity (meq/g): 0.310

Application:

Scavenger for acids, cycliccompounds, cholesterols, and otherlipid-type and compounds.



Application:




Application:



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MOLECULAR

ANALYTE CHARGELOG P > 3 LOG P < 2

CN, NH2,PSA,

PHENYL,PI

GAS

SAMPLE

SOLIDLIQUID

LIPIDS, OIL ADDITIVES, CARBOHYDRATES,PHENOLS, OIL SOLUBLE VITAMINS,

PESTICIDES, HERBICIDES

DRUGS OF ABUSE, PHARMACEUTICALS,TDM, PESTICIDES

PARTITION COEFFICIENT

Po/w= (Coil /Cwater) EQUILIBRIUM. OIL IS USUALLY OCTANOL

LOG P SCALE

HYDROPHILIC (POLAR) < 1

MODERATELY POLAR 1-2

HYDROPHOBIC (NONPOLAR) > 2

TYPICAL APPLICATIONS:

+ -

CARBON SILICA GELFLUOROSIL

DIOL

LOG P < 1

NON POLAR, REVERSE PHASE EXTRACTION

AQUEOUS, BIOLOGICAL FLUIDS, BUFFERS

HYDROPHOBIC, LOG P > 2.5

INVOLVES VANDERWALLS/DISPERSION FORCES

INTERACTION BETWEEN SORBENT CH BONDS AND

ANALYTE CH BONDS

PROTONATED/NEUTRAL STATE,

AROMATICS, ALKYLCHAINS

POLAR OR NORMAL PHASE EXTRACTION

NON POLAR ORGANIC

HYDROPHILIC, LOG P < 2.5

INVOLVES HYDROGEN BONDING

AND DIPOLE/DIPOLE INTERACTIONS

AMINES, HYDROXYLS, CARBONYLS,

AROMATIC RINGS, HETERO ATOMS (O, S,

N, P)

NATUREOF SAMPLE

TYPES

OF FUNCTIONAL

GROUPS

BONDING

MECHANISM

NATURE

OF ANALYTE

MATRIX

TYPE

OF EXTRACTION

ANALYTE

CONFIGURATION

ANALYTE

POLARITY

SORBENT

TYPE

NON POLAR TO MODERATELY POLAR SOLVENTSWATER/ACETONITRILE/METHANOL MIXES

MEDIUM TO HIGH POLARITY SOLVENTSMETHANOL/ETHYL ACETATE/ACETONE/THF

MIXES

ELUTION SOLVENT:

A MAP TO OPTIMIZING SAMPLE CLEAN UP

C8- C30PS/DVD

C2- C7CYCLOHEXYL

ALUMINA


37/80U C


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CLEAN-UP


QUATERNARY AMINE WITH CHLORIDE COUNTER ION


Tube Volume Pack

CUQAX1L1 50mg/1mL 100CUQAX111 100mg/1mL 100CUQAX123 200mg/3mL 50CUQAX153 500mg/3mL 50CUQAX156 500mg/6mL 50CUQAX1M6 1g/6mL 30CUQAX12Z 200mg/10mL 50CUQAX15Z 500mg/10mL 50CUQAX12M15 2g/15mL 20

QUATERNARY AMINE WITH ACETATE COUNTER ION


Tube Volume Pack

CAQAX111 100mg/1mL 100CAQAX123 200mg/3mL 50CAQAX1M6 1g/6mL 30CAQAX15M25 5g/25mL 20

QUATERNARY AMINE WITH HYDROXIDE COUNTER ION


Tube Volume Pack

CHQAX1L1 50mg/1mL 100CHQAX111 100mg/1mL 100

CHQAX123 200mg/3mL 50CHQAX153 500mg/3mL 50CHQAX156 500mg/6mL 50CHQAX1M6 1g/6mL 30CHQAX12Z 200mg/10mL 50CHQAX12M15 2g/15mL 20CHQAX15M25 5g/25mL 20

POLYIMINE


Tube Volume Pack

CUPAX123 200mg/3mL 50CUPAX153 500mg/3mL 50CUPAX1M6 1g/6mL 30



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe acetate counter ion.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe hydroxide counter ion.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and otherelectrophiles.

ANY CHARGED COMPOUNDS

IONIC

2 BCX, PCX &COPOLYMERS

CATIONSANIONS

STRONG STRONG

QAX &COPOLYMERS

CCX, TAX, &COPOLYMERS

MUST BE ABLE TO BE CHARGED

AQUEOUS OR ORGANIC

ION EXCHANGE

INVOLVES IONIC EXCHANGE

ACIDS AND BASESANIONS: PHOSPHATES, CARBOXYLIC ACIDS,

SULFONIC ACIDSCATIONS: AMINES, PURINES, PYRIMIDINES

NATUREOF SAMPLE

TYPES

OF FUNCTIONAL

GROUPS

BONDING

MECHANISM

NATURE

OF ANALYTE

MATRIX

TYPE

OF EXTRACTION

ANALYTE

CONFIGURATION

ANALYTEPOLARITY

SORBENT

TYPE

BRISTOL, PA 19007

800-385-3153

www.unitedchem.com

2731 BARTRAM ROAD

UCT, Inc.

ACIDIC ELUTIONSOLVENT

BASIC ELUTIONSOLVENT

USING SOLID PHASE EXTRACTION

TYPICAL APPLICATIONS:

ELUTION SOLVENT:

NH , DEAE,PSA, PI, &

COPOLYMERS

WEAK WEAK


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CLEAN-UP


QUATERNARY AMINE WITH CHLORIDE COUNTER ION


Tube Volume Pack

CUQAX1L1 50mg/1mL 100CUQAX111 100mg/1mL 100CUQAX123 200mg/3mL 50CUQAX153 500mg/3mL 50CUQAX156 500mg/6mL 50CUQAX1M6 1g/6mL 30CUQAX12Z 200mg/10mL 50CUQAX15Z 500mg/10mL 50CUQAX12M15 2g/15mL 20

QUATERNARY AMINE WITH ACETATE COUNTER ION


Tube Volume Pack

CAQAX111 100mg/1mL 100CAQAX123 200mg/3mL 50CAQAX1M6 1g/6mL 30CAQAX15M25 5g/25mL 20

QUATERNARY AMINE WITH HYDROXIDE COUNTER ION


Tube Volume Pack

CHQAX1L1 50mg/1mL 100CHQAX111 100mg/1mL 100

CHQAX123 200mg/3mL 50CHQAX153 500mg/3mL 50CHQAX156 500mg/6mL 50CHQAX1M6 1g/6mL 30CHQAX12Z 200mg/10mL 50CHQAX12M15 2g/15mL 20CHQAX15M25 5g/25mL 20

POLYIMINE


Tube Volume Pack

CUPAX123 200mg/3mL 50CUPAX153 500mg/3mL 50CUPAX1M6 1g/6mL 30



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe acetate counter ion.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe hydroxide counter ion.



Application:

Scavenger for acids and sulfonylchlorides, isocyanates and otherelectrophiles.


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CLEAN-UP

CATION EXTRACTION COLUMNS

CARBOXYLIC ACID


Tube Volume Pack

CUCCX1L1 50mg/1mL 100CUCCX111 100mg/1mL 100CUCCX123 200mg/3mL 50CUCCX153 500mg/3mL 50CUCCX156 500mg/6mL 50CUCCX1M6 1g/6mL 30CUCCX12Z 200mg/10mL 50CUCCX12M15 2g/15mL 20CUCCX15M25 5g/25mL 20

PROPYLSULFONIC ACID


Tube Volume Pack

CUPCX111 100mg/1mL 100CUPCX123 200mg/3mL 50CUPCX153 500mg/3mL 50CUPCX156 500mg/6mL 50CUPCX12Z 200mg/10mL 50CUPCX15Z 500mg/10mL 50

TRIACETIC ACID


Tube Volume Pack

CUTAX111 100mg/1mL 100CUTAX123 200mg/3mL 50CUTAX153 500mg/3mL 50CUTAX156 500mg/6mL 50CUTAX1M6 1g/6mL 30CUTAX110M75 10g/75mL 10



Application:

Scavenger for strong anions(Quaternary amines or metals)



Application:

Scavenger for amines, alcohols andother nucleophiles.


Exchange Capacity: Anion: 0.17 Cation: 0.06Application:

Chelator for metal ions. i.e. tin palladium copper ruthinium chromium

nickel


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CLEAN-UP

CATION EXTRACTION COLUMNS

BENZENESULFONIC ACID


Tube Volume Pack

CUBCX1L1 50mg/1mL 100CUBCX111 100mg/1mL 100CUBCX123 200mg/3mL 50CUBCX153 500mg/3mL 50CUBCX156 500mg/6mL 50CUBCX1M6 1g/6mL 30CUBCX11Z 100mg/10mL 50CUBCX12Z 200mg/10mL 50CUBCX15Z 500mg/10mL 50CUBCX12M15 2g/15mL 20CUBCX110M75 10g/75mL 10

BENZENESULFONIC ACID - HIGH LOAD


Tube Volume Pack

CUBCX1HL1L1 50mg/1mL 100CUBCX1HL11 100mg/1mL 100CUBCX1HL23 200mg/3mL 50CUBCX1HL53 500mg/3mL 50CUBCX1HL56 500mg/6mL 50CUBCX1HLM6 1g/6mL 30CUBCX1HL1Z 100mg/10mL 50CUBCX1HL2Z 200mg/10mL 50CUBCX1HL5Z 500mg/10mL 50CUBCX1HL2M15 2g/15mL 20CUBCX1HL5M25 5g/25mL 20CUBCX1HL10M75 10g/75mL 10



Application:




Application:



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This sorbent is composed of a silica back-

bone with two types of functional chains

attached - an ion exchanger or polar chain

and a hydrophobic carbon chain. Our

copolymeric phases are produced in a way

to allow for equal parts of each functional

group to attach to the silica backbone. Thiscopolymerization technique yields reproducible

bonded phases and unique copolymeric

chemistries which allow the controlled use

of mixed mode separation mechanisms.This

type of dual chemistry is beneficial especially

when one is looking for both a neutral &

charged compound. This is common when a

neutral parent drug metabolizes & becomes

a charged compound.

Cations/Anions

Alkanes

Alkenes

Aromatics

1) Aqueous to disrupt

hydrophilic interactions.

2) Methanol to disrupt residualhydrophobic and hydrophilicinterferences.

1) Organic, possibly with some

aqueous to elute hydrophobicallybound analytes.

2) Aqueous buffer with a pH thatwould neutralize ionically boundanalytes or an aqueous with highionic strength or a solvent with acounter ion that would bindto sorbent.

CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS

Example of a Copolymeric Phase

Silica Backbone

Hydrophobic Chain

Ion Exchanger

O

Si

O

Si

O

Si

O

Si

O Si (CH2)3 NH+3

OH

OH

CnH2n+1Si

OH

OH

O

Analytes Washes Elutions


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CLEAN-UP

COPOLYMERIC EXTRACTION COLUMNS

COPOLYMERIC SORBENTS & STRUCTURES

Sorbent Structure* pKa

Benzenesulfonic Acid (BCX2) -Si-(CH2)

2 SO

3H always charged

Strong Cation Exchange Column

Propylsulfonic Acid (PCX2) -Si-(CH2)

3SO

3H < 1

Strong Cation Exchange Column

Carboxylic Acid (CCX2) -Si-(CH2)

2COOH 4.8

Weak Cation Exchange Column

Quaternary amine (QAX2) -Si-(CH2)

3N+(CH

3)

3 always charged

Strong Anion Exchange Column

Aminopropyl (NAX2) -Si-(CH2)

3NH

3 + 9.8

Weak Anion Exchange Column

Cyanopropyl (CNP2) -Si-(CH2)3CNHydrophilic Exchange Column

Cyclohexyl (CYH2) -Si-(CH2)

Hydrophobic Exchange Column

*Each copolymeric sorbent also contains a carbon chain approximately equal to a C8 chain

MECHANISM OF COPOLYMERIC BONDING

Using a sample composed of a theoretical neutral parentdrug and its charged (acidic) metabolite, it is appliedat a pH of 6 (figure 1). At this pH, many amine groups are

positively charged. Since the column is also positivelycharged, compounds with this chemistry (cations)are repelled. Depending on the pKa of the metabolite,carboxylic acid groups may be negatively charged, allowingthe metabolite to bond to the positively charged sorbent.Since the column also possesses a hydrophobic chain,the neutral parent drug also bonds to the column.

Water or a weak aqueous buffer (pH6) washes awayhydrophilically bound interferences (figure 2). The columnis then dried, careful to free the column of any residualaqueous phase that would interfere with elution.

Example of Copolymeric Bonding

(CH2)n NH3+ CO

O

SorbentCompounds


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CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS

Sample Applicationgure 1

Column Washgure 2

Elution 1gure 3

The hydrophobicallybound neutral parentdrug is eluted with asolvent of minimalpolarity, such ashexane/ethyl acetate -80:20.

Elution 2gure 4

The final elutionemploys an acid toneutralize the chargeof acidic analytes. Ionicinteraction is released,and analytes are elutedin an appropriate solventmixture.

NH3+

CO

O

O

NH3+

NH+

NH+

O

-

Sorbent

Sorbent

Sorbent

Metabolite

Aqueous Buffer

Parent Drug

NH3+

CO

O

O

NH3

SH

HO

OH

+

-Sorbent

Sorbent

Sorbent

Metabolite

Aqueous Buffer

Hydrophilically

Bound

CompoundsParent Drug

NH3+

CO

O

NH3+

O

-

Sorbent

Sorbent

Sorbent

Parent Drug

Hexane / Ethyl

Acetate

80:20

NH3+

CHO

O

NH3+

Sorbent

Sorbent

Sorbent

Metabolite

Strong Acidic

Solution

pH 2-3

Anion Exchange Sorbent Cation Exchange Sorbent

Goal pH Goal pH

WASH

Elution

Percent of Compound in Ionic State

Functionality Ionization

To promote

bonding between

sorbent and analyte

pH units away from pKa

2 < pKa 1 < pKa at pKa 1 > pKa 2 > pKa

Acid Anionic (-) 1 9 50 91 99

Base Cationic (+) 99 91 50 9 1

To disrupt

bonding between

sorbent and analyte

To promote

bonding between

sorbent and analyte

To promote

bonding between

sorbent and analyte

> analyte pKa

or

< sorbent pKa

> analyte pKa

or

< sorbent pKa

> analyte pKa

or

< sorbent pKa

> analyte pKa

or

< sorbent pKa


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CLEAN-UP


HYDROPHOBIC PLUS CYCLOHEXYL


Tube Volume Pack

CUCYH256 500mg/6mL 50CUCYH2M6 1g/6mL 30

HYDROPHOBIC PLUS PROPYLSULFONIC ACID


Tube Volume Pack

CUPCX2L1 50mg/1mL 100CUPCX211 100mg/1mL 100CUPCX223 200mg/3mL 50CUPCX256 500mg/6mL 50CUPCX22Z 200mg/10mL 50

HYDROPHOBIC PLUS CARBOXYLIC ACID


Tube Volume Pack

CUCCX2L1 50mg/1mL 100CUCCX211 100mg/1mL 100CUCCX223 200mg/3mL 50CUCCX256 500mg/6mL 50

HYDROPHOBIC PLUS BENZENESULFONIC ACID


Tube Volume Pack

CUBCX2L1 50mg/1mL 100CUBCX211 100mg/1mL 100CUBCX223 200mg/3mL 50CUBCX253 500mg/3mL 50CUBCX256 500mg/6mL 50CUBCX2M6 1g/6mL 30CUBCX21Z 100mg/10mL 50CUBCX22Z 200mg/10mL 50CUBCX25Z 500mg/10mL 50

OCTADECYL PLUS BENZENESULFONIC ACID


Tube Volume Pack

CUBCX35Z 500mg/10mL 50CUBCX32M15 2g/15mL 20


Exchange Capacity (meq/g): N/A

Application:

Dual functionality for phenols andhydrophobic compounds.



Application:

Dual functionality for weak basesand hydrophobic compounds.



Application:

Dual functionality for strong base andhydrophobic compounds.



Application:




Application:



45/80U C


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CLEAN-UP


HYDROPHOBIC PLUS QUATERNARY AMINE


Tube Volume Pack

CUQAX2L1 50mg/1mL 100CUQAX211 100mg/1mL 100CUQAX223 200mg/3mL 50CUQAX253 500mg/3mL 50CUQAX256 500mg/6mL 50CUQAX2M6 1g/6mL 30CUQAX21Z 100mg/10mL 50CUQAX22Z 200mg/10mL 50CUQAX25Z 500mg/10mL 50



Application:

Dual functionality for weak acids andhydrophobic compounds.

HYDROPHOBIC PLUS AMINOPROPYL


Tube Volume Pack

CUNAX2L1 50mg/1mL 100CUNAX211 100mg/1mL 100CUNAX223 200mg/3mL 50CUNAX253 500mg/3mL 50CUNAX256 500mg/6mL 50CUNAX2M6 1g/6mL 30CUNAX21Z 100mg/10mL 50CUNAX22Z 200mg/10mL 50CUNAX25Z 500mg/10mL 50CUNAX22M15 2g/15mL 20CUNAX25M25 5g/25mL 20CUNAX210M75 10g/75mL 10



Application:

Dual functionality for strong acidsand hydrophobic compounds.


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CLEAN-UP

COVALENT EXTRACTION COLUMNS

COVALENT

Covalent sorbent has aldehyde functional groups that are bound to the silicabackbone by a hydrocarbon chain. The aldehyde group will react selectively

with compounds containing a primary amine. A formal bond is createdbetween the stationary support and the primary amine containing material.

MECHANISM OF COVALENT BONDING

The primary amine in the sample performs a nucleophilic attack on the aldehydefunctional group attached to the support. This results in a Schiff base, with theamine immobilized on the stationary support. This chemistry can be utilized tobind proteins, such as antibodies, to the support, allowing highly specific extractions.

THIOPROPYL


Tube Volume Pack

CUTHX1L1 50mg/1mL 100CUTHX111 100mg/1mL 100CUTHX123 200mg/3mL 50CUTHX153 500mg/3mL 50CUTHX156 500mg/6mL 50CUTHX1M6 1g/6mL 30CUTHX11Z 100mg/10mL 50CUTHX12Z 200mg/10mL 50CUTHX15Z 500mg/10mL 50CUTHX12M15 2g/15mL 20CUTHX15M25 5g/25mL 20CUTHX110M75 10g/75mL 10


Application:

Scavenger for alkylating agents,alcohols and amines.


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STYRE SCREEN POLYMERIC RESIN EXTRACTION COLUMNS


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STYRE SCREEN

POLYMERIC RESIN EXTRACTION COLUMNS

Styre Screenextraction columns contain an ultra clean, highly cross-linked styrene and divinylbenzene copolymersorbent that is functionalized with both a reverse phase, hydrophobic component and a strong cation exchanger.High and reproducible recoveries for acidic, neutral and basic compounds are achievable with a single column. TheStyre Screenparticles have an average particle size of 30 microns and a very high analyte capacity making them idealfor standard solid phase extraction applications. The increased analyte capacity means that less sorbent bed mass isneeded which results in faster flow rates and less solvent use. Higher throughput and less solvent waste disposal

translate into significant savings in both time and money. In addition, no conditioning steps are required for mostdrugs of abuse applications.

DBX - BENZENESULFONIC ACID + C18


Tube Volume Pack

SSDBX031 30mg/1mL 100SSDBX033 30mg/3mL 50SSDBX056 50mg/6mL 50

DVB - POLYSTYRENE DIVINYLBENZENE

Part Number Sorbent Amount/ Unit per Tube Volume Pack

SSDVB031 30mg/1mL 100SSDVB033 30mg/3mL 50SSDVB056 50mg/6mL 50

BCX - ION EXCHANGE


Tube Volume Pack

SSBCX031 30mg/1mL 100SSBCX033 30mg/3mL 50SSBCX056 50mg/6mL 50

C18 - REVERSE PHASE


Tube Volume Pack

SSC18031 30mg/1mL 100SSC18033 30mg/3mL 50SSC18056 50mg/6mL 50

CCX - CARBOXYLIC ACID


Tube Volume Pack

SSCCX031 30mg/1mL 100SSCCX033 30mg/3mL 50SSCCX056 50mg/6mL 50

QAX - QUATERNARY AMINE


Tube Volume Pack

SSQAX031 30mg/1mL 100SSQAX033 30mg/3mL 50SSQAX056 50mg/6mL 50

Application:

Dual functionality for weak acidsand hydrophobic compounds.

Application:N/A

Application:

Scavenger for amines, alcoholsand other nucleophiles.

Application:

Removes hydrophobic impurities,de-salting and purification ofhydrophobic compounds.

Application:

Scavenger for strong anions.(Quaternary amines or metals)

Application:



49/80U C


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SOLID PHASE EXTRACTION COLUMN METHOD DEVELOPMENT KITS

UCT understands that the optimum

sorbent for any given separation

cannot always be chosen empirically

and that the cost of purchasing

individual sorbents for screening

purposes can be prohibitive. We offer

the following kits at prices designed

to reduce the cost of assay development:

Non-Polar Phases

Polar Phases

Ion Exchange Phases

Copolymeric Phases Pharmaceutical Phases

Toxicology Phases


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SOLID PHASE EXTRACTION COLUMN

METHOD DEVELOPMENT KITS

NON-POLAR PHASES, ENDCAPPED

Part Number: MDK-NPE-I Total Number of Tubes: 140 7 packages / 10 each

CEC18111 CECn4111 CEC08111CECYH111 CEPHY111CEC02111 CEC30111

POLAR PHASES

Part Number: MDK-PU-I Total Number of Tubes: 80 8 packages / 10 each

CEC02111 CUDOL111 CUPSA111CUCNP111 CUSIL111 CUDAX111CECNP111 CUNAX111

COPOLYMERICPart Number: MDK-PU/1EX-11 Total Number of Tubes: 60 6 packages / 10 each

CUCNP211 CUCCX211CUNAX211 CUPCX211CUQAX211 CUBCX211

ION EXCHANGE PHASES

Part Number: MDK-IEX-I Total Number of Tubes: 70 7 packages / 10 each

CUNAX111 CUCCX111

CUPSA111 CUPCX111CUDAX111 CUBCX111CUQAX111

TOXICOLOGY PHASES

Part Number: MDK-TOX-111 Total Number of Tubes: 40 8 packages / 5 each

CSDAU203 CEC02123CSTHC203 CEC08123CUSIL123 CEC18123CECNP123 CUC18123

PHARMACEUTICAL PHASES

Part Number: MDK-PHM-111 Total Number of Tubes: 40 8 packages / 5 each

CEC08123 CEC18123CUBCX223 CUCCX123CUNAX223 CUQAX123CEC02123 CUCNP123

This kit contains ten 100 mg/1 ml tubes of each of seven non-polar phaseswhich include the endcapped hydrophobic phases, for C2, C4, C8, C18 and C30along with cyclohexyl (CYH), and phenyl (PHY) phases.

This kit contains ten 100 mg/1 ml tubes of each of eight phases with polarcharacteristics, including C2, cyanopropyl (CNP), diol (DOL), silica (SIL),primary amine (aminopropyl; NAX), secondary amine (aminoethyl; PSA),tertiary amine (diethylamino; DAX).

This kit contains ten 100 mg/1 ml tubes of each of six mixed mode phases containing anon-polar and polar or ion exchange functionality. The non-polar functionality in each casehas an effective chain length of a C8. The polar or ion exchange functionality of each phaseconsists of one of the following: cyanopropyl (CNP2), primary amine (aminopropyl; NAX2),quaternary amine (QAX2), carboxylic acid (CCX2), propylsulfonic acid (PCX2), andbenzenesulfonic acid (BCX2).

This kit contains ten 100 mg/1 mL tubes of each of seven ion exchange phases including

primary amine (aminopropyl; NAX), secondary amine (aminoethyl; PSA), tertiary amine(diethylamino; DAX), quaternary amine (QAX), carboxylic acid (CCX), propylsulfonic acid(PCX), and benzenesulfonic acid (BCX) phases.

This kit contains five 200 mg/3 ml tubes of each of eight phases. The two standard phasesused for drugs of abuse testing are CSDAU and CSTHC. Other phases commonly used intoxicology are the polar and non-polar phases. The polar phases are unbonded silica andcyanopropyl (CNP); the non-polar phases are endcapped, C2, C8, and both endcapped andunendcapped C18.

This kit contains five 200 mg/3 ml tubes of each of eight phases that are most often selectedfor pharmaceutical applications. Two copolymeric phases having the dual functionalities ofnon-polar C8 and either benzenesulfonic acid (BCX) or aminopropyl (NAX). The remainingcolumns are phases which include three endcapped, hydrophobic phases (C2, C8, C18), twoion exchange phases (CCX, QAX), and one polar phase (CNP).


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SELECTRASORB

BULK SORBENTS PACKING MATERIAL

COPOLYMERIC BONDED PHASESFOR DRUG ABUSE TESTING

Description Part Number Sizes

CSDAU

CSDAU00X 10g CSDAU00C 100g CSDAU00K 1kg

CSTHC

CSTHC00X 10g CSTHC00C 100g CSTHC00K 1kg


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SELECTRASORB

BULK SORBENTS

HYDROPHOBIC

Description Part Number Part Number Sizes

Endcapped Unendcapped

C8, OCTYL

CEC0800X CUC0800X 10g CEC0800C CUC0800C 100g CEC0800K CUC0800K 1kg

C18, OCTADECYL

Tri-Functional

CEC1800X CUC1800X 10g CEC1800C CUC1800C 100g CEC1800K CUC1800K 1kg

HYDROPHILIC



CN,CYANOPROPYL

CECNP00X CUCNP00X 10g CECNP00C CUCNP00C 100g CECNP00K CUCNP00K 1kg

UNBONDED SILICA

40-63 m CUSIL00X 10g CUSIL00C 100g CUSIL00K 1kg

PHARMA-SIL

40-63 m PHSIL00X 10g PHSIL00C 100g PHSIL00K 1kgHigh Surface Activity Silica

40-63 m HSSIL00X 10g HSSIL00C 100g HSSIL00K 1kg

DIOL

CUDOL00X 10g CUDOL00C 100g CUDOL00K 1kg

FLORISIL

CUFLS00X 10g CUFLS00C 100g CUFLS00K 1kg

ALUMINA, ACIDIC

CUALA00X 10g CUALA00C 100g CUALA00K 1kg

ALUMINA, BASIC

CUALB00X 10g CUALB00C 100g CUALB00K 1kg

ALUMINA, NEUTRAL

CUALN00X 10g CUALN00C 100g CUALN00K 1kg

COPOLYMERIC



BENZENESULFONIC

ACID + C8

CUBCX20X 10g CUBCX20C 100g CUBCX20K 1kg

CARBOXYLIC

ACID + C8

CUCCX20X 10g CUCCX20C 100g CUCCX20K 1kg

QUATERNARY

ACID +

Documents

Uct Tipos de Columna