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Spot tests tospot the cheats

By: INSERT NAME


What is a spot test?

A spot test is …a simple chemical reaction that allows a substance to be identified.

Heroin Cocaine

In drug testing…• a drug or class of drugs gives a characteristic visible change

when mixed with another chemical (the reagent)• a sample, such as urine, is tested with the reagent• the presence of the drug (or class of drug) is evidenced by the

characteristic visible change; this is a positive test• if the characteristic change is not seen, it is a negative test


B CA

Now it’s your turn …

You have been given three different urine samples from three different athletes: a cyclist (A), an archer (B) and a tennis player (C)

You have two different reagents (labelled Y and Z)•Reagent Y gives a characteristic red colour if a steroid drug is present•Reagent Z gives a characteristic blue colour if a beta-blocker is presentUse the reagents to identify which athletes have taken a banned substance


Which two athletes have taken a banned substance?

Which class of banned substance did each athlete take?

Do your results agree with one another?

1. Place the culture plate on a white piece of paper2. Mark the columns and rows, as in the diagram3. Place a drop of urine sample A into the top two wells in column A4. Place a drop of urine sample B into the top two wells in column B5. Place a drop of urine sample C into the top two wells in column C6. Add drops of reagent Y to row Y, and drops of reagent Z to row Z

What you have to do

Work in pairs. One of you should use the tophalf of the well plate. The other should repeatthe procedure in the bottom half.


• are quick and easy to use• are cheap (no complex, expensive equipment needed)• allow selection of samples for further analysis• can be performed outside the lab

Spot tests: the pros

They …

The colour identifies the class of drug. The intensity of the colour is an approximate measure of how much of the drug is present.


• usually only indicate the class of substance (identification of the actual substance requires a different test)

• don’t tell you how much of the banned substance is present• don’t give a permanent record of the test result

Spot tests: the cons

Modern instruments can identify the actual drug present, an accurate measure of how much of it is present, and they provide a permanent record.

They …



GlaxoSmithKlineand London 2012

By: INSERT NAME


The Olympic Games

the most recognised brand in the world

the world’s biggest audience - four billion

10 500 athletes across 205 countries

eight million tickets

26 sports

70 000 volunteers


The Paralympic Games

the second biggest event in the world - audience of 1.8 billion

20 sports

471 medal events

two million tickets

4200 athletes across162 countries


Congrats Team GB!

Olympics

Paralympics


GlaxoSmithKline Brought Science to the Games …

GSK formed a ground-breaking partnership with King’s College London to help make London 2012 the cleanest Games possible …

the anti-doping laboratory operated all day, every day

4910 samples were tested for the Olympic Games – 3870 urine and 1040 blood

more than 1200 tests were carried out for the Paralympic Games

… that’s more tests than any other Games

GSK and London 2012


Official Laboratory Services Provider

Making London 2012 the fairest Games possible will be crucial in making the Games a success. As a company with science at its heart, GlaxoSmithKline is perfectly placed to provide a world class laboratory, in partnership with King’s College, with the capability to carry out more doping tests than ever before. Science plays an integral role in sport and I hope the London 2012 Games inspires more young people to take up roles in sport science related careers.

Lord Seb Coe, Chair of the London Organising Committee of the Olympic Games and Paralympic Games

“

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GSK and London 2012


Check out this YouTube channel for videos …

GSK and London 2012

GSK Vision London 2012:http://www.youtube.com/playlist?list=PLA2D719CFD3F27508

Visit the Scientists in Sport website for more info …

Scientists in Sport:http://www.scientistsinsport.com

Documents

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