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张迺蘅 张迺蘅 张迺蘅 张迺蘅教授给孙小依的信 教授给孙小依的信 教授给孙小依的信 教授给孙小依的信: 缅怀健的几句话 小依: 因健康状况限,我不能参健博士的纪念活。上几句纪念文字下: 健博士于上世纪 80 年代后期曾来我们的科研组从事博士后研究。他工作异常苦,日 以继夜埋在实验室里。他反应敏锐,独立工作能很突出,是位极有发展途的青年科学家。后来 他有机会出国深造,我理所当然地给予支持。此后我一直关注着他在国外工作的进展。果然不出所 料,历经数年他在人乳瘤病毒研究上取得优异成绩,为宫颈癌疫苗的研作出重大贡献。我为他的 杰出成就深感骄傲,为他的英年早逝极为痛心。 张迺蘅教授 原京医科大学校长、生系主任 中国京 2008 1 31 张迺蘅近照于中国京。Nai Heng Zhang in Bei Jiang, 2008

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Page 1: zhou jian-3b

张迺蘅张迺蘅张迺蘅张迺蘅教授给孙小依的信教授给孙小依的信教授给孙小依的信教授给孙小依的信::::

缅怀周健的几句话

小依:

因健康状况限制,我不能参加周健博士的纪念活动。奉上几句纪念文字如下:

周健博士于上世纪 80年代后期曾来到我们的科研组从事博士后研究。他工作异常刻苦勤奋,日

以继夜埋头在实验室里。他反应敏锐,独立工作能力很突出,是位极有发展前途的青年科学家。后来

他有机会出国深造,我理所当然地给予支持。此后我一直关注着他在国外工作的进展。果然不出所

料,历经数年他在人乳头瘤病毒研究上取得优异成绩,为宫颈癌疫苗的研制作出重大贡献。我为他的

杰出成就深感骄傲,为他的英年早逝极为痛心。

张迺蘅教授

原北京医科大学副校长、生化系主任

中国北京

2008年 1月 31日

张迺蘅近照于中国北京。Nai Heng Zhang in Bei Jiang, 2008

Page 2: zhou jian-3b

周健周健周健周健,,,,你是我们的骄傲你是我们的骄傲你是我们的骄傲你是我们的骄傲 ——在周健博士逝世 9周年之际的回忆

徐英含i

周健的硕士学位论文获浙江省 1985 年度科技进步一等奖,周健在河南的出色成就《人民日报》

曾以头版作了报道,周健在澳大利亚发明宫颈癌预防性疫苗,周健始终不忘中国根——“我是中国培

养出来的土博士”,这一切的一切,难道不值得我们为之骄傲吗!

我从 1978 年起,每年招收一位硕士研究生,周健是我的第五位研究生。我一生只收过十四位研

究生,他们个个事业有成,现在担任大学校长的就有两位,周健在其中尤为突出,但非常遗憾,他却

英年早逝。今天,我只能饱含眼泪,缅怀他的过去,以寄托我的哀思。

择优录取择优录取择优录取择优录取,,,,周健是优中之优周健是优中之优周健是优中之优周健是优中之优

每年报考我的研究生的人数颇多,为了不耽误他们的录取机会,有些考生我还介绍他们报考其

他导师。我的招生原则是,不管报名人数多少,我每年只招一个,并且不分男女,不计年龄,不论本

校他校,一律择优录取。1982 年,孙小依医师陪同周健来找我,他们都是温州医学院毕业的。我的

印象是,孙医师较会说话,因为周健的情况是由她来介绍的。周健是个瘦高个子,两只眼睛炯炯有

神。那时外语和政治是统一命题的,只有专业是导师命题的。专业考试的题型有几种,我注意问答

题,因为在问答题中我不仅可以了解考生对专业的熟悉程度,而且可以知道其行文的逻辑性、条理

性。周健的外文和专业笔试成绩都是第一,这样我就放心招他了。我可以预料,他今后的工作会有条

不紊。他写论文时表达能力强,还可以用外文写论文寄国外权威杂志发表。

严格要求严格要求严格要求严格要求,,,,周健是品学兼优周健是品学兼优周健是品学兼优周健是品学兼优

在科研事业中,一切均应实事求是,凡浮夸风、报喜不报忧、弄虚作假都是不允许的。我非常注

意研究生在科研工作中的科学态度,其中之一是抓他们的科研档案。我每天要查他们的实验记录。周

健的实验记录非常详尽,以日志的形式逐日把当天的工作记下,既记录试验成功的数据,也记录失败

的、与预期结果不符的数据。只有这样,才能改进实验,最后完成实验。他的实验记录完整、清晰。

当时,以杨光华教授等为主的卫生部研究生教育检查团来我校检查,最后他们的评语是,我校最好的

研究生在我们这里,其中就包括周健。

在科研工作中必须勤奋。周健在我这里,从 1982 年到 1985 年的整整三年中,不分寒暑假,不分

日夜,工作起来,一杆子到底,做好为止。有时因为细胞培养需要,不仅白天要在实验室观察,连晚

上也需要不间断地进行观察,周健正是这样地在实验室度过了许许多多个夜晚。

我们的实验室在当时还是条件不差的,有专用的实验室,一大两小,还有专用的细胞培养室,

仪器设备应有尽有。但是在这样的条件下,我们还是要合理使用科研经费。每购买一件仪器,我们师

生总是商量又商量,精打细算。那时我们还要办一个全国性科研培训班,人数较多,仪器设备就不够

用了。周健动手能力很强,他看到学校里有报废的电冰箱、离心机、孵箱等,就设法去要来,加以修

理和油漆,居然和新的一样。

办全国巨噬细胞科研培训班办全国巨噬细胞科研培训班办全国巨噬细胞科研培训班办全国巨噬细胞科研培训班,,,,周健是骨干周健是骨干周健是骨干周健是骨干

当时在国内,我们研究肺泡巨噬细胞的形态和功能处于领先地位。肺泡巨噬细胞的形态、功能

变化和大气污染密切相关。为了推动这项研究在全国范围内开展,为此我们在 1984 年举办了两期培

训班,每期数十人,来自全国各地,有病理学界的,也有防疫部门的;有教授,也有一般的医生。培

训的内容主要有:从灌洗兔肺泡巨噬细胞开始到如何在体外培养,从观察形态变化到检测其功能变

Page 3: zhou jian-3b

化,等等。这个班的讲课主要是周健,指导实验主要也是周健,连他们的生活安排也主要靠周健,因

为学员们的住宿也是通过周健的妈妈帮助解决的。

工作生活工作生活工作生活工作生活,,,,亲如一家亲如一家亲如一家亲如一家

因为我们的工作作风非常合拍,所以师生关系非常融洽。我们非常注意工作效率和计划性,对

工作要么不接受,一旦接受就不能拖拉,工作完成只能提前,不能推后。白天在工作室中,我很少和

大家聊天,大家都说我这个人太严肃,或者说有点怕我,但周健能理解我,不怪我。我们在工作时一

本正经,但在工作之余就比较随便,那时我经常留周健在我家里吃饭,我也有时到周健家里坐坐,聊

聊家常。我们亲如一家人,我至今还保存着一张在他家沙发上和他以及高他二届的研究生一起坐着聊

天的黑白照片。

周健的学位论文发表于美国权威杂志周健的学位论文发表于美国权威杂志周健的学位论文发表于美国权威杂志周健的学位论文发表于美国权威杂志,,,,获浙江省科技进步一等奖获浙江省科技进步一等奖获浙江省科技进步一等奖获浙江省科技进步一等奖

我从 1978 年开始,一直在进行肺泡巨噬细胞体外培养的研究,所以我每年招的研究生也让他们

在这个范围内选题。周健是我的第五届研究生,对于他的学位论文选题,我给他提了两个范围,一个

是我在 50 年代曾进行铅中毒动物实验,一个是当时正在进行的肺泡巨噬细胞的研究,希望他能将两

者结合起来研究。周健阅读了我过去发表的论文,又查阅了近年发表的有关文献,很快他就提出“铅

离子在体外对肺泡巨噬细胞免疫功能的影响”作为其论文选题。他在论文设计中,不仅沿用我们实验

室传统的实验方法,他认为铅离子对肺泡巨噬细胞免疫吞噬功能的影响迄今未见报道,因为在体外,

肺泡巨噬细胞不易吞噬大颗粒物质如鸡红细胞,因此很少用鸡红细胞观察铅离子对肺泡巨噬细胞功能

影响的研究。周健通过预试验,提出应用抗血清介导肺泡巨噬细胞吞噬鸡红细胞,从而提高吞噬指

数。在检测肺泡巨噬细胞吞噬指数的方法学方面,他除应用传统的光镜下计数外,还采用同位素标记

法,使检测更为灵敏、简便、可靠。他的论文发表于 Toxicology and Applied Pharmacology。除此

之外,他又完成了两篇论文,分别发表于《中华劳动卫生职业病杂志》和《浙江医科大学学报》。在

周健离开我这里后,我组合了他的论文、另一研究生的论文和我自己的工作申报浙江省科技进步奖,

荣获 1985 年度浙江省科技进步一等奖,周健列名第一,我列名第三。这是我校研究生的殊荣。

我没有能留住周健而遗憾我没有能留住周健而遗憾我没有能留住周健而遗憾我没有能留住周健而遗憾,,,,但我为他的辉煌成就而高兴但我为他的辉煌成就而高兴但我为他的辉煌成就而高兴但我为他的辉煌成就而高兴

周健在我这里快结束学业时,他去报考河南医科大学沈琼教授的博士生。当时我很想留住他在我

这里工作,但是他有远大的抱负,我不能阻止他。记得那时他的老父亲来找我,希望我能说服他留下

来,我说我已经做了,但是我要尊重他自己的意见。从今天来看,他当时的选择是正确的。

正是通过周健的辛勤工作,呕心沥血的研究,终于人类历史上第一个癌症疫苗——子宫颈癌疫

苗问世。子宫颈癌是女性生殖系统中最常见的恶性肿瘤,发病率占女性生殖系统恶性肿瘤的半数以

上,死亡率居我国女性生殖系统恶性肿瘤的首位。早年我的母亲和我的伯母就是死于子宫颈癌的。今

天,周健博士等发明的疫苗将造福于人类,新疫苗的使用有可能在一代人中根除子宫颈癌。我为周健

博士在人类征服肿瘤史中作出巨大的贡献而由衷地高兴。

痛悼周健英年早逝痛悼周健英年早逝痛悼周健英年早逝痛悼周健英年早逝

1999 年 3 月的一天,我接到周健姐姐的电活,周健因肝昏迷逝世于杭州 117 医院,我当时如五

雷轰顶,接受不了这个突如其来的事实。因为他这次回杭州,我事先不知道,所以我没有能在他生前

去医院看他,只有在追悼会上向他的遗体告别,这成为我终生的遗憾!

以周健逝世前的成就,如能再假以天年,他在医学上一定还能创造出一个一个的奇迹。老天爷,

你真太残忍了,夺去了还在英年的周健的生命。

周健,安息吧!你出色的成就给人类带来健康和幸福,澳大利亚政府为表彰你的突出贡献,拨款

为你组织隆重的纪念活动,这是你的光荣。我,一个 82 岁的老人,你过去的导师,为曾有你这样一

位出色的学生而感到骄傲。

Page 4: zhou jian-3b

徐英含(前排中间)、周健(后排右)、张洪福(后排左)等,1984 年于杭州

Ying Han Xu (front middle), Jian Zhou(back right) and Hong Fu Zhang (back left)

i徐英含教授, 浙江杭州浙江大学医学院

Page 5: zhou jian-3b

Dr Jian Zhou: Our Pride

Yinghan Xui

It has been nine years since Dr Jian Zhou left us. We are proud of his medical breakthrough on cervical cancer

vaccine which has the huge potential to win a future Nobel Prize in Medicine as claimed by Time Magazine.

It is even more significant as Dr Zhou is the first PhD educated in Mainland China to achieve such a hard-

earned glory. Dr Zhou has always been very proud of his Chinese heritage even though most of his world

class medical research was done overseas. As Dr Zhou’s Master Degree supervisor, I have always been

impressed by his hard work and diligence. Dr Zhou’s Master Degree thesis was awarded the best research

achievement in 1985 by the Scientific Academy of Zhejiang Province. Dr Zhou’s early success in medical

research was reported by China Daily before he started his research at Cambridge in 1989.

The best student

I have supervised 14 post-graduate students for Master’s Degree in my academic life since 1978. I prefer

quality to quantity therefore I only take in one post-graduate student per-year based only on their merit. They

have all achieved success in their field and two of them are current university Chancellors. Dr Zhou was my

fifth student but he is unarguably the best in academic achievement. Dr Zhou’s early departure is my biggest

regret.

Dr Zhou studied medicine at Wenzhou Medical School, graduating in 1982. He came to me the same year to

enquire about post-graduate study under my supervision. He was a young energetic man with tall slim build

accompanied by his wife Dr Sun. I took him under my wing after he passed the national entry exam with high

distinction in both general and academic subjects. He had showed huge potential in medical research in the

way he has analysed and solved the academic questions designed by me in the exam.

The brilliant young researcher

A good scientific researcher requires the virtue of honesty, diligence and excellent observation skills. One of

the most important aspects is the ability to write and summarize the laboratory records daily. Dr Zhou had a

brilliant eye for scientific detail. He has showed the excellent ability to design and conduct experiments as

well as to record and analyse laboratory data. Dr Zhou was one of my best post-graduate students.

From 1982 to 1985, Dr Zhou had worked very hard in my laboratory. Sometimes he had worked day and

night in order to conduct a series of experiments which require continuous observation involving cell cultures.

He has sacrificed many weekends and holidays for his research. Dr Zhou has also showed his extraordinary

ability to modify and convert discarded domestic equipments such as refrigerators, centrifuges and incubators

into laboratory use in order to conduct experiments with limited budget and meeting the high demands of the

national research training work-shop.

The key member of macrophage research

In 1980’s my laboratory played a leading role in the research of the morphology and function of alveolar

macrophage nationally. The research has found a close relationship between the level of environmental

pollution and the changes in macrophage cells in the lungs, which has national significance. In order to

advance the research into this field, we have conducted an extensive training course nationally in 1984. More

than 100 experts in pathophysiology, public health and general medicine attended the course. Dr Zhou was the

key lecturer as well as the key organizer.

The friendship

We have forged a very good relationship as mentor and apprentice because we both shared a passion for

medical research. We devoted our effort to plan and work efficiently with excellent work ethics. I have set

strict laboratory rules and appeared stern while at work. Dr Zhou has enjoyed working in my laboratory

because of our mutual understanding. We have also enjoyed a good relationship as friends. We have visited

each other’s house on the weekends and shared laughs together.

Page 6: zhou jian-3b

Thesis published on <Toxicology & Applied Pharmacology>

I have started research on the alveolar macrophage culture in vitro since 1978. All my post-graduate students’

research was involved in this area. As my fifth Master’s student, Dr Zhou’s research was focused on lead and

alveolar macrophage. Based on my research of lead poisoning on animal model in 1950’s, Dr Zhou designed

his Master research on the effects of lead ion on alveolar macrophages after intensive literature review. He

was the first to conduct research on the effects of lead ion on the immune function of alveolar macrophages.

The research was published on <Toxicology & Applied Pharmacology> titled “The effects of lead ion on

immune function of rabbit alveolar macrophages: quantitation of immune pathagocytosis and Fc-rosette

formation by 51Cr in vitro”. Dr Zhou has also published two research articles on this topic in <Chinese

Journal of The Preventive Medicine> and <The Journal of Zhejiang Medical School> respectively. Our

research in this area has been awarded “the best research achievement” in 1985 by the Scientific Academy of

Zhejiang Province.

Successful career

After Dr Zhou finished his Master research in my laboratory, I was really hoping that he could have stayed on

to assist my research. His father also wished that he would continue his career in his hometown. Instead, Dr

Zhou started his PhD study with Professor Sun at Henan Medical School to advance his career. I understood

his move and his ambition in medical research.

The world’s first cervical cancer vaccine was created after Dr Zhou’s life long hard work and research.

Cervical cancer is one of the most common cancers which has killed many women. My grand mother and

mother were both died from cervical cancer. Dr Zhou’s vaccine makes it possible for us to wipe it out in the

future. I am really happy that Dr Zhou has made an incredible medical breakthrough and great contribution to

human wellbeing.

Early departure

I received a unexpected phone call from Dr Zhou’s sister and learnt of his sudden death in March 1999. Dr

Zhou died at the 117 Hospital, Hangzhou from septic shock. I wish I would have visited him at the hospital

before his tragic departure.

As a young man, Dr Zhou has achieved incredible success in medical research. He would have made even

greater contribution to medical research and human wellbeing if his life had not been cut short.

Dr Zhou, the world are a better place because of your great contribution to humanity, you can sleep peacefully.

The Australian government has set up a fund in your name in honour of your contribution. I, am 82 years old

chap and your Master’s supervisor, I am really pride of you – the best student ever!

i Professor Yinghan Xu, Medical School, Zhejiang University

Page 7: zhou jian-3b

谷淑燕给孙小依的信谷淑燕给孙小依的信谷淑燕给孙小依的信谷淑燕给孙小依的信::::忆周健忆周健忆周健忆周健

小依:你好!

写纪念文章真是难为我了, 我这个人表达能力极差。但作为周健博士的朋友,老师早就不敢当

了,在如此重要的时刻,不做点甚么,从心里实在过意不去。如果不能表达出周健博士的真实一面,

岂不如不写。矛盾之下,我回忆与周健博士的相识,相处和从他身上,不论是科研工作或待人处世所

受到的启发,点点滴滴,供你参考。

20 年前,我和周健博士相识,缘为人乳头瘤病毒。1983 年,我从德国慕尼黑 Hans Wolf 教授的实

验室带回来几十株人乳头瘤病毒 DNA 的克隆,包括与人肿瘤相关的 6、11、16 和 18 等型别。我曾用

原位核酸杂交和间接核酸杂交方法,检测出尖锐湿疣和宫颈癌组织细胞中存在人乳头瘤病毒 DNA,并

在国内首先发表了论文。我的研究课题是 EB 病毒和鼻咽癌,没有继续人乳头瘤病毒的研究。1986

年,周健在北京等我从德国回来,告诉我,打算扩展他的博士研究课题,研究人乳头瘤病毒与食道癌

发生的关系。当时,在国内能开展分子生物学工作的实验室很少;而当时在国内,惟只有我采集了多

个型别的人乳头瘤病毒 DNA 样本。周健说服了我,在我的实验室继续人乳头瘤病毒的研究。就这样,

我们的缘分从人乳头瘤病毒开始。

我在德国期间,曾和 Hans Wolf 教授共同整理了一本实验室操作方法(Laboratory Manual)作

为 Course on Molecular Virology,1983-1985 年曾在北京、广州等地学习班上作教材,它是周健

实验操作的主要参考书。他非常努力,非常勤奋,很快掌握了书中介绍的方法,利用我们实验室的条

件顺利地完成了课题。在实验室里他总是来得最早,走得最晚。经常是清晨传达室还没开大门,他已

经来了;半夜值班人员已经睡了。他才离开,所以我经常要和值班人说对不起。在实验室里他从不把

自己当外来人,总是做尽量多的事情,例如打扫卫生,送洗实验器材等等,还奋不顾身地扑灭超净台

失火。超净台是负压送风,酒精溢出,台面立即起火,电机开着非常危险,在场的人不知所措,是周

健找来灭火器,避免了一场灾难。

我们经常在一起讨论工作,互相学习实际工作中的经验和方法。他很善于动脑筋,总能想办法解

决遇到的问题,Elu-tipd columns 是当时纯化核酸的工具,国内买不到,从德国带回来的有限,用

一个少一个,他就想办法,处理回收再用。

我们的友谊就是从实验室开始的,表面上他是博士生,我是老师,实际上我们是很好的朋友。多

年来不论他在英国、美国、澳大利亚,我们都保持着书信联系,交流科研进展。他经常在信纸上画质

粒图和标克隆位点。发表了论文马上寄给我,有时还是杂志社的清样。我有一个专门的文件夹,存放

他和孙小依的信件,至今仍保留着。

我对周健博士的帮助其实只是提供了人乳头瘤病毒 DNA 样本和实验室条件。但是他总是记得这一

点点帮助,即使是他在科研工作中已经做出很大成绩和贡献时。记得在一次学术报告中,他讲人乳头

瘤病毒颗粒及感染上皮细胞的机理。在报告会上他说我是他永远要记住的老师,我很感动,不是因为

他记住我,而是因为他的为人,一个优秀科学家的谦和品质。他去英国剑桥后我曾收到 Lionel

Crawford 教授的信,感谢推荐如此优秀的人到他实验室工作。1990 年 Lionel Crawford 教授写信告

诉我周健博士去澳大利亚,非常舍不得,后来 Lionel Crawford 教授还曾让我帮助了解一个申请去他

实验室的中国人的情况。可见周健不论在那里,都会很出色,都是给人留下好的记忆。在他和孙小依

的安排下,我曾去布里斯班,在他的研究所介绍 EB 病毒基因工程疫苗人体免疫的结果。回国时,他

们为我准备了难以找到的实验用品和一些资料如那本 Antibodies A Laboratory Manual.,非常细

心周到。

多年来我从周健博士那里得到的帮助很多。我们的友谊扩展到两个家庭。周健和孙小依对我、皮教

授和我们的女儿总是充满着关爱,让我体会到年龄差别,成绩和贡献大小不影响真诚的友谊。他们对

皮小燕就像自己的小妹妹,他们送她的红鼻子博士陪她走进大学。他们千里迢迢从美国买来的

“Molecular Biology of the Cell” 她至今保留着。周健不在了,我们和孙小依和周妈妈多年来一

直保持联系,我们每年必定收到小依的贺卡。

他在杭州病故后,我去杭州见他最后一面。他走得太突然,一走就是这么多年,但很多美好的记

忆和友谊让我一直珍藏在心里,回忆起来历历在目,这是一个有成就的好人留给我们的财富。

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谷淑燕

原中国医学预防科学院(现中国疾病预防控制中心)病毒所教授

1997 年于布里斯本植物园,左起周健和谷淑燕。 From left: Jian Zhou and Shu Yan Gu in

Brisbane,1997.

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A Letter to Xiaoyi Sun: Memories of Jian Zhou

Dear Xiao Yi,

As a friend of Dr Jian Zhou and his former supervisor, it is my great honor to write some words in memory of

him. At the moment, all the memories regarding Jian Zhou are coming back to me. I would like to recall how

we met and what I had learned from him when we worked together. I would also like to say something about

his attitude towards his research work, his inter-personal relationship skills that had inspired me.

Jian and I became to know each other due to a project of Human Papilloma Virus (HPV) about 20 years ago.

I brought back several dozens of HPV DNA clones including 6, 11, 16 and 18 types which are related to the

human tumors from the Prof. Hans Wolf’s Lab in Germany examined the HPV DNA of papilloma wart and

cervical cancer tissue using the methods of nucleic acid in situ hybridization and indirect nucleic acid

hybridization. I then published the first research paper on this area in China. However as my major research

project at that time was Epstein-Barr virus (EB virus). I did not continue the research on the HPV. In 1986,

Jian intended to expand his PhD research project to study the relationship between the HPV and esophageal

cancer. At that time, there were only a few biochemical labs in China, and only myself had many types of

HPV DNA. He was in Beijing to wait for my coming back from Germany. I was persuaded by his

envisagement, and our close personal relationship started from the HPV.

During my stay in Germany, Prof. Hans Wolf and I co-authored a Laboratory Manual which was used widely

as a teaching material in Molecular Virology in Beijing, Guangzhou, and some other places from 1983 to

1985. That was the major operating protocol applied by Dr. Jian Zhou. Worked very hard and diligently, Jian

became familiar with the operation quickly and successfully completed his project in our laboratory. He was

always the first one coming to the laboratory and the last one leaving. He even very bravely helped to

extinguish an accidental fire in the laboratory.

We usually learned something new from each other. We also exchanged ideas and discussed experimental

work and the problems we faced in research. He was really good at problem solving. Elu-tipd columns are a

tool to purify nucleic acid, and it could not be purchased in China at that time. However, Jian Zhou found a

way to recycle them for re-use.

Our friendship was formed and built up in the laboratory. Outwardly, he was a PhD student and I was a

supervisor, but inwardly we were good friend. In the following years, no matter he was in Britain, America,

and Australia, we still kept in touch and wrote to each other, exchanging research development. Whenever

Jian published a paper, he always sent me a copy immediately. I now still keep a file of research reports and

letters from him and his wife, Xiao Yi.

The only help I gave to Jian was that I provided him HPV and the lab equipments. He always remembered

and appreciated this help, even when he made great achievement and contribution in his research work. I

remembered that once Jian Zhou explained the mechanism of the HPV pellet and infection of epithelial cell in

an academic lecture, he respected that I always was his teacher. I was deeply touched by his attitude – the

humble moral character of an outstanding scientist. After he arrived at Cambridge University, I received a

letter from Prof. Lionel Crawford, thanking me for recommending him such a unique person. Prof. Lionel

Crawford also informed me in 1990 that Dr. Zhou moved to Australia to work, and he felt very regret for his

leaving.

Jian Zhou and his wife made an arrangement for me to visit his Laboratory in Brisbane and introduced

Epstein-Barr virus gene engineering and humoral immunity of vaccine. They also helped me to obtain some

lab equipments and useful materials which were not available in China, such as Antibodies – A Laboratory

Manual.

I obtained a lot of assistance from Jian Zhou in the past years, and our friendship developed between our two

families. He and his wife cared about my husband, me, and our daughter. The age difference and the

achievement would not affect our friendship at all. They treated our daughter like their sister, and saw her

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growing from a child to a university student. They bought “Molecular Biology of the Cell” in the United

States and brought it back to China for her. Although Jian Zhou was passed away, we still keep in touch with

Xiao Yi and his mother. We still receive greeting card from Xiao Yi every year.

After Jian Zhou passed away in Hangzhou, I went there and saw him for the very last time. His sudden

leaving has been for many years up to now, but the beautiful memories are always vivid in my mind. I would

like to say that this is the “wealth” passed to us by an extraordinary person, a person with a great achievement

in his field.

Shuyan Gu

Professor of Beijing Virus Research Institute, the Chinese Medical Preventive Academy Sciences

————————————————————————

English translator: Mr. Ron Li, Brisbane, Australia

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我的师兄周健博士我的师兄周健博士我的师兄周健博士我的师兄周健博士

常福聚常福聚常福聚常福聚ii

From left Xiao Yi Sun, Jian Zhou and Fu Qu Chang at HPV international Conference in Edinbrough, UK. 1992年

十月于英国爱丁堡的 HPV国际学术会上,左起孙小依、周健及常福聚。

1986年,我和周健在河南医科大学相识。那时,他是我的师兄,我们同在沈琼教授的指导下攻读博

士。我们的导师是中国食管癌细胞学创始人,专门用组织和细胞学方法研究食道癌。周健打破了沈琼

教授的形态病理学的研究传统,而从事人乳头状病毒(HPV)的分子生物学研究。无论白天黑夜,他

天天在实验室做试验。在短短的二年内,周健作出了巨大的成绩。沈琼教授很佩服他的工作,对他的

确很重視,并将我推荐给他。从此,我跟周健作助手,跟他学分子生物学技术。他手把手地教我,而

且帮我从北京和杭州买分子生物学方面的书籍。他是我的师兄,也是我的科学启蒙人。

博士毕业后,我们继续做 HPV方面的研究。周健先后到北京、英国、澳洲和美国等分子生物学试

验室工作。每到一处,他都给人们留下了美好的印象。

1988年,周健在剑桥大学的英国帝国癌症研究基金会 (ICRF) 的病毒实验室做研究,成为国际 HPV

研究的先驱 Lionel Crawford教授接收的第一位中国研究员。Crawford教授很喜欢他,认为他是一个很

有创造性的年青人。

周健的夫人孙小依医生也是一位勤学上进的研究员。他们不但是一对彼此相爱的夫妻,也是一对配

合默契、勤奋能干的研究人员。1990年,应 Ian Frazer教授的邀请,周健夫妇到昆士兰大学他所在的

实验室一起工作。在澳洲,他们用 HPV的 L1和 L2晚期蛋白,在体外成功地合成了世界上第一例克

隆 HPV病毒样颗粒。这个人工合成的 HPV病毒样颗粒里面不含病毒 DNA内核,所以没有感染性,

但外壳上有很多抗原,进入身体后就会刺激免疫系统而产生抗体,因此它本身就是一个很好的疫苗。

这一发明为 HPV疫苗的研制奠定了基础。

周健和 Frazer 教授将这一成果发表在 1991 年第 185 期的《病毒学》期刊上。1991 年 6 月,昆

士兰大学将这项发明成果申请了专利,当年 7 月,俩人在美国西雅图举行的乳头状病毒国际会议上报

告了这项成果。动物试验成功之后,又开始转入临床试验。

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我于 1988 年去了芬兰,在 Syrjanen 教授的试验室作 HPV 研究工作。我们经常在 HPV 国际会议

上相遇(见图 1 和 2)。每次相逢,我们都是十分的高兴,总是要一起庆贺。

1999 年,听说周健突然去世的消息,这对我真是十分的震惊。年仅 42 岁,他的发明创造刚刚得

到 HPV 研究界的公认。我们需要他,整个 HPV 研究界需要他。用基因工程手段生产的病毒样颗粒不含

病毒感染成分、却能刺激人体产生免疫反应,从而有效地控制 HPV 感染,这是人类医学史上的一项重

大突破。在他的研究基础上,目前许多国家已将 HPV 疫苗应用于青年妇女,以防止 HPV 感染引起的子

宫痉癌。遗憾的是,周健却未能亲眼看到这项研究成果在临床上大规模应用和在人类抗肿瘤中的巨大

作用。

(2007年 12月于英国伦敦)

i常福聚博士 Dr. Fuju Chang, MD, MSc, PhD,Consultant Cytopathologist & Histopathologist,

Department of Histopathology,St Thomas' Hospital,Guy's & St Thomas' NHS Foundation Trust,UK