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osor
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•– –
•– –EL
ISA
ELIS
AEL
ISA
ELIS
A
Agr
aQua
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ELIS
AG
C-E
CD
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
Agr
aQua
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ELI
SA
ELI
SA
Agr
aA
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uant
Qua
nt®®
• • •ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
• • • •
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
• • •
•– – – – – – – –
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
• Ant
ibod
y co
ated
mic
row
ells
)
–
• (non
-coa
ted
dilu
tion
mic
row
ells
)
– –
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
•(M
ycot
oxin
stan
dard
( –
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
• (Myc
otox
inco
njug
ate)
– –
• (Sub
stra
te a
nd S
top
solu
tion)
–(T
MB
)– –
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
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•20
015
0–
DO
N
•10
0–
•N
o.1
–
ELI
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ELI
SA
Agr
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Qua
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•–
100
L20
0L
1L
•–
100-
200
L
•–
•–
•–
•–
•–
(450
nm)
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
•– –
•– –
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
Agr
aQua
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28
•4
•30
• • •18
• •
Agr
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DO
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w/v
L
1
L
• • •3
MP
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abs
Agra
Qua
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flato
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(1-2
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NTIT
ATI
VE
KIT
D
ata
Redu
ctio
n W
orks
heet
Ope
rato
r:TL
MD
ate:
6-N
ov-0
2A
ssay
ID:
Ana
lysi
s of
soy
sam
ples
Pla
te L
ot#:
SIN
-02-
0053
Con
j. Lo
t:S
IN-0
2-00
54S
tds
Lot:
SIN
-02-
0046
, 005
5-00
59S
ubst
rate
Lot
:S
IN-0
2-00
78S
top
Lot:
SIN
-02-
0079
Bio
Rad
Rea
der
Sect
ion
I: C
alib
ratio
n C
urve
Std
. Lev
elAb
s.B
/Bo
Log(
Con
c.)
Logi
t B/B
o0
ppb
=2.
274
1.0
ppb
=2.
039
0.90
0.94
R^2
=-0
.994
92.
5 pp
b =
1.74
90.
770.
400.
52Sl
ope
=-1
.191
55.
0 pp
b =
1.36
30.
600.
700.
17In
terc
ept =
0.96
1510
.0 p
pb =
0.72
70.
321.
00-0
.33
20.0
ppb
=0.
501
0.22
1.30
-0.5
5
Sect
ion
II: S
ampl
e C
alcu
latio
ns
No.
ABS
SAM
PLE
logi
t B/B
opp
bAv
g. p
pbSt
dev
12.
274
0 pp
b st
d#D
IV/0
!#D
IV/0
!2
2.03
91.
0 pp
b st
d0.
941.
053
1.74
92.
5 pp
b st
d0.
522.
344
1.36
35.
0 pp
b st
d0.
174.
575
0.72
710
.0 p
pb s
td-0
.33
12.0
96
0.50
120
.0 p
pb s
td-0
.55
18.5
27 8
1.58
4S
ampl
e 3
mix
ed0.
363.
199
2.40
5S
ampl
e 3
mix
ed#N
UM
!#N
UM
!10 11
1.88
1S
ampl
e 1
0.68
1.72
122.
207
Sam
ple
21.
520.
3413
2.17
8S
ampl
e 3
1.36
0.47
141.
741
Sam
ple
40.
512.
3715
0.54
7S
ampl
e 1
spik
ed 1
0 pp
b to
tal A
fla-0
.50
16.8
316
0.60
5S
ampl
e 2
spik
ed 1
0 pp
b to
tal A
fla-0
.44
15.0
317
0.59
7S
ampl
e 3
spik
ed 1
0 pp
b to
tal A
fla-0
.45
15.2
618
0.53
2S
ampl
e 4
spik
ed 1
0 pp
b to
tal A
fla-0
.52
17.3
519 20
2.18
1S
ampl
e 1
1.37
0.45
212.
261
Sam
ple
22.
240.
0822
2.21
3S
ampl
e 3
1.56
0.31
231.
462
Sam
ple
40.
263.
9124
0.61
7S
ampl
e 1
spik
ed 1
0 pp
b to
tal A
fla-0
.43
14.6
925
0.54
6S
ampl
e 2
spik
ed 1
0 pp
b to
tal A
fla-0
.50
16.8
626
0.64
3S
ampl
e 3
spik
ed 1
0 pp
b to
tal A
fla-0
.40
14.0
1
-0.8
0
-0.6
0
-0.4
0
-0.2
0
0.00
0.20
0.40
0.60
0.80
1.00
1.20
0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40
Log(
Conc
.)
Logit(B/B0)
• •
•–
•– –
•–
•–
Agr
aQua
nt®
Agr
aQua
ntA
graQ
uant
®®A
flaA
fla
Afla
toxi
nB1 B2 G
1G
2G
IPS
AA
graQ
uant
Afla
4/40
(Lot
# S
IN(A
)01-
0001
)
104
50g
Afla
toxi
n B
1, B
2, G
1, G
2
(ppb
)20
31
1
100
11.6
527
.46
100
42.4
%
Agr
aQua
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graQ
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flaA
fla
Agr
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flaA
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41
4G
IPSA
414
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250
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421
371
Agr
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41
4G
IPSA
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250
g1
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421
371
Agr
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flaA
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GIP
SA
1 (L
ot#
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34
13
Agr
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fla
Agr
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flaA
flaA
graQ
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Agr
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Afla
Afla
Agr
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Agr
aQua
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graQ
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ON
DO
N
• •
ELIS
A
TLC
0.5
0.7
GC
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DA
OA
C0.
9
HPL
C0.
9
CV
48.1
40.3
44.7
120
.216
.718
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.19.
514
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80.1
78.4
79.4
105
105
105
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98.2
99.5
0.99
760.
9983
0.99
78
5.7
1.2
4.3
n=105
n=81
.....
DO
ND
ON
n=178
DO
ND
ON
CV
1
18.8
23
.2
31.7
25.9
21.4
16.6
19.6
13
.0
•
CV
1
11.7
24.8
16.1
15.5
11.7
23.4
17.0
11.0
.....
n=159
n=85
n=40
n=15
91.3
96.0
84.9
73.2
93.0
97.3
77.2
96.8
91.4
85.0
105
83.0
94.2
97.1
73.0
106
n=40
n=15
ND
Fals
e po
si.
3.4
0
Fals
e po
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9.4
0
Fals
e ne
ga.
131.
2
•
ND
Fals
e po
si.
2.5
Fals
e po
si.
0.0
0
10
0
Fals
e ne
ga.
0
.....
n=159
n=85
fals
e n
egat
ive
n=83
• CV
AB
C
13.2
15
.2
10.5
15
.2
11.0
16
.2
9.1
12.8
11.1
11
.6
10.3
13
.5
AB
C
118
124
110
105
121
117
99.9
113
118
107
112
108
AB
C
ND
Fals
e po
sitiv
e0
6.7
02.
3
Fals
e ne
gativ
e0
00
0
Fals
e po
sitiv
e0
00
0
Fals
e ne
gativ
e0
00
0
•A
BC
53
2
CV
4.1
19.6
7.4
CV
1 16.5
21.9
25.0
13.8
18.8
17.2
19.1
20.9
32.4
19.4
21.1
40.6
n=80
919
Agr
aQua
ntA
graQ
uant
®®A
flaA
fla
®
®
4mL
300.
6mL
Agr
aQua
ntA
graQ
uant
®®A
flaA
fla
Myc
oSep
®#1
12
ELI
SA
ELI
SA
Agr
aA
gra Q
uant
Qua
nt®®
////
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