RNA-Seq による

ゲノム情報の大規模解析とカスタム分析

1

東京農工大学「農学系ゲノム科学人材育成プログラム 」

特任教授　石井一夫

BGI ウェビナー：2014年6月13日(金)　14:00~15:00

本日の内容

１、自己紹介に代えて: ビッグデータ環境によるデータ解析系の構築 ２、RNA-Seqの概要、問題点その対策 ３、今後の展開 基本的にUNIXコマンドを使い始めたばかりの初心者を想定してお話します。 本スライドの内容は、SlideShareに公開しますので、 スクリプトなどが欲しい方はそちらからダウンロードしてください。

3

ビッグデータ環境によるデータ解析系 の構築

我々のビッグデータ処理の新しい産業応用

個別化医療（ライフサイエンス）： 　精神神経系疾患 （うつ病、総合失調症、双極性感情障害）の 　　　　　　　　　　　　　　　　網羅的ゲノム診断法の開発 ゲノム育種（グリーンサイエンス）： 　イネなどの新品種の開発 環境アセスメント（エコサイエンス）： 　環境微生物の分布、分類、生態調査 　

データの増加

6

1,000,000,000

1,000,000,000,000

1,000,000,000,000,000

1,000,000,000,000,000,000

1,000,000,000,000,000,000,000

ゲノム科学におけるビッグデータ分析 本研究室では、大規模データ解析に対し以下の4方法で対応している。 HPCも、モンテカルロも、クラウドも使えるものは何でも使う主義。 1. モンテカルロシミュレーション：大量データから無作為に サンプルを抽出し、元の全体データをシミュレーションして推測。 2. HPC、Big iron (大容量メモリサーバ)による大量並列処理 HP社の協力により、4TBメモリ、CPU: Xeon E7 (80 コア、 160スレッド)の大容量メモリ解析サーバを使用、 理研、「京」互換SCLS 3. Hadoop による分散処理システム（クラウドを利用）on AWS Amazon Elastic MapReduce（Amazon EMR）プラットフォーム 4. Hadoop によらない分散処理システム シェルスクリプトベースの分散処理。 usp-BOA （USP研究所）を利用。

１、モンテカルロシミュレーション乱数発生により大量データの一部を抽出し、全体の性状を推定特に、新規に機器を買う必要がなくすぐに気軽に実施できるので、結果をすぐに出したいときに頻用。

２、BIG IRON, ハイパフォーマンスコンピューティング

(HPC)の利用

理研（「京」互換スパコンSCLSシステム）の利用

理化学研究所HPCI講習会資料より

ヒューレットパッカード社の大容量メモリーサーバー利用

80コア、4TBメモリ

３、Hadoop による分散処理システム（クラウドを利用）on AWS

BIG　Data 処理のためのフレームワーク

Hadoop = HDFS + MapReduce

Hadoop File System (HDFS) コンピュータクラスタ上に、分散させて ファイルを保存 →　分散ファイルシステム これにより、ペタバイト、エクサバイト級のファイルの扱いが可能になる。

15

MapReduce 複数のプロセスを、 コンピュータクラスタ内の複数のコンピュータに 分散させて処理

17

MapReduce

個人レベル、１研究室レベルで コンピュータクラスタを使うには

AWS(Amazon Web Service)の利用

アマゾンウェブサ＝ビス（公共担当　吉荒様）よりの提供資料

アマゾンウェブサービス（公共担当　吉荒様）よりの提供資料

22

Unicage Cluster (BOA) (BOA: Bigdata Oriented Architecture)

4. Hadoop によらない分散処理システム シェルスクリプトベースの分散処理

23

Unicage Cluster

Basic Cluster Configuration

boam

boas001

Bubun file system

Infini-band High Speed Network

boas002 boas00n

Shell Script is written only on this server

Master Server

… …

Bubun file system

Bubun file system

Bubun file system

Bubun file system

Slave Servers

Master server: - Unix/linux OS - Unicage commands - Custom shell, ush - Bubun File System Slave server: -Unix/linux OS - Unicage commands - Custom shell, ush - Bubun File System

Perform actual processing

USP研究所2013年米国MIT発表資料より

Human DNA Sequance Quality Check

- Data: FASTQ sequences of human genome a person's DNA sequence is about 35GB 1: @ABCD:77:64U9X:6:3:11306:1000 2:N: 2: TTCCAGTACTTCCGCCAGGCACG 3: +B-=DFFBB<BF2:=*68B?BD>DCF=@=? 4: @AE3A?:BB2B<BB+B:8C7=<##### /Data resource : Prof. Ishii at Tokyo University of Agriculture and Technology/

Quality Score

DNA Base Squence Squence Name

Squence Name

USP研究所2013年米国MIT発表資料より

非定形データ

定形データ

数理モデル化

説明変数の選択

モデル最適化

モデル検証

ビッグデータ解析のワークフロー例 Hadoop MapReduceを用いた並列分散処理、 シェルスクリプトを用いた分散テキスト処理、 NoSQL によるデータ処理、モンテカルロ法による無作為抽出

RDMS（MySQL、PostgreSQLなど）によるデータ処理、集計

統計学的有意差検定（ステューデントのt検定、マン ホイットニーのU検定）、スパースモデリング

疾患患者の識別 判別分析、サポートベクトルマシン、機械学習、ベイズフィルタリング

線形モデル、ロジスティック回帰モデル、両者の混合モデル など

ウィルクスラムダ、AIC（赤池情報量規準）、BIC（ベイズ情報量基準）など

クロスバリデーション（リーブワンアウト法を含む）

26

２、RNA-Seqの概要、問題点その対策

RNA-Seq

1. Reference がある場合 　1.1　発現定量解析　臨床データ、モデル生物 　1.2　RNA多型解析　臨床データ、モデル生物 2. Reference がない場合 　2.1　発現定量解析　非モデル生物 　2.2　RNA多型解析　非モデル生物 small RNAについては方法などが異なるので今回は 除外します。主に発現定量解析に限定して議論します。

BGI JAPAN website より 転載

Referenceありの場合の RNA-Seq ワークフロー

Referenceがない場合のRNA-Seq ワークフロー

BGI JAPAN website より 転載

RNA-Seq を始めるにあたって

検出感度、反復回数、網羅性、コントロールなど 基本的問題を考えてみます。

問題点：検出感度（検出限界）

経験的な一般論として、 検出感度 　　RNA-Seq ＜ マイクロアレイ　＜　定量PCR 網羅性 　　RNA-Seq ＞ マイクロアレイ　＞　定量PCR RNA-Seq はマイクロアレイや定量PCRに比べて 検出感度がよくない傾向。検出感度 を上げるためには多くのリード数が必要。

問題点：リード数

リード数が必要はどれだけ必要か？ 大体、最低でも1000万リードはあったほうがよい。 １種類の生物に約10000個の遺伝子があったと仮定して 1000万リードのデータがある場合、 平均して１遺伝子あたり、1000リードの配列データが マッピングされる。解析の目的にもよるが、数千万リード は必要な場合が多い。

検出感度

検出感度ギリギリの遺伝子の定量は困難。 リードカウントが１0以下では離散型データになり変動比などの 比較が困難

　　　　　　　　　　　　

統計学的な評価をする場合の反復回数の問題

反復回数が１〜２回の場合：　統計学的評価（検定は困難） 反復回数が少ない場合（３〜５回）： 　 Student t検定では、大量の擬陽性データが検出される。 　 Mann-Whiteney U検定では最低でも、４回以上の反復がないと、 　　有意差は出ない。 臨床検体など、検体数が多く確保できる場合を除き、一般的な基礎実験 では、RNA-Seqで統計な議論をすることは困難。 RのパッケージなどでDEG(発現変動遺伝子)を検出してくれるものもある が、現実問題としては他の方法（例えば、qPCRなど）で確認することが 望ましい。 DEGやFDRなどの問題については書籍「トランスクリプトーム解析」 門田幸二、共立出版（2014）などに詳しいので、そちらを参照してください。 ここでは、深くは立ち入りません。

実験デザインの問題（基本の基本なのですが、、）

１、ネガティブコントロールを入れ忘れる人が結構います。 例えば、ある生物（細胞）に対するAという薬剤とBという薬剤の効果を 比較したい場合、薬剤処理群だけのデータをとり、薬剤非処理群のデー タをとり忘れるケースです。 相対的な定量値で判断しますので、ネガティブコントロールは必須です。 実験デザイン上、常にネガティブコントロールを意識する必要があります。 ２、実際のデータの解析でも、ネガティブコントロールを無視したような 解析、解釈をする人がいます。 上記の例のAという薬剤とBという薬剤の処理群の絶対量のみで比較 しようとするケースです。

サンプルの質の問題（これも、基本の基本なのですが、、）

採取した生物、細胞のクオリティ、RNA のクオリティなど、配列解析 を実施する材料のバリデーションなどはきちんと行ったほうがいいと 思います。 １、バイアビリティの悪いと思われる細胞や、クオリティの悪いと見られる サンプルを用いて解析を行い、？？？な結論を出していると思われる例 もたまに見られます。 ２、材料のコンタミやサンプルの取り違えなどが、 シーケンス後に検出される場合も。

メタゲノムまたは、2種類以上の生物の混じった検体のRNA-Seq

種によって特異的な遺伝子の定量は可能。 種によって配列の類似する遺伝子の定量は、困難。 メジャーな生物の遺伝子変動に飲み込まれ、マイナーな生物 の遺伝子の変動をみることは困難。 A A A G G メジャーな生物とマイナーな生物の間の多型を手がかりに定量

メジャーな生物

マイナーな生物

一般的な RNA-Seqの ワークフロー

http://bioinfo.mc.vanderbilt.edu/ NGS/rna-seq.htmlより

データ解析の方針

自作スクリプト、コマンド　＞　フリーソフト　＞　商用ソフト 基本的に車輪の再発明を奨励しています。 自作スクリプトやコマンドで処理できるようにしておけば、 いろいろな場面で応用が効くようになるので、 いろいろな解決法を身につけておいたほうがいいと思います。 各研究者の事情によりますがLinux環境での使用を奨励して います。 また、実際の解析の際には、単にプログラムのみでなくウェットの 実験のセンスや経験も必要になってきます。 ウェブでの情報も充実してきていますので、独学でもかなり できるようになります。

クオリティチェック

解析結果に結構影響が出ますので、必要な行程です。 FastQC, HTQC, qrpc, ShortRead, FASTXToolkitなどが使えます。 クオリティチェックは、FastQCの操作そのものが易しいのでこれを使います。 インストール法： wget http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/fastqc_v0.8.0.zip unzip fastqc_v0.8.0.zip cd Fastqc chmod 755 fastqc インストール後fastqcのパスを通します 使用法： ワイルドカードが使えますので、コマンド一行で、ファイル内の複数データを一気に全部 処理できます。 fastqc　/path/to/*.fastq

クオリティコントロール

クオリティの悪いデータを除く行程です。 cutadapt, FASTX-toolkit, PRINSEQ, ShortReadなどが使えます。 ただし、この行程は、シェルのコマンドや、Perl, Python, Rubyなどのスクリプトで 簡単に自作できます。また、そのほうがいろいろ融通が利きます。 私たちは、cutadaptを使うほか、自作スクリプトで対応しています。 その他の問題として： マルチプレックスのサンプルでデマルチプレックスを失敗しているケースがあります。 デマルチプレックスはegrepコマンドを用いたコマンド１行で、自在にできますので、 それで処理します。

市販のソフトによるクオリティチェック、クオリティコントロールなど

CLCゲノミクスワークベンチなどの市販のソフトでも可能です。 きめ細かな、クオリティチェック、クオリティコントロールのため には、自作スクリプトやコマンドのパイプラインが強力ですので、 慣れてきたら、FastQCでざっとクオリティをチェックして、 作り捨ての一行コマンドで 除いてしまったほうが早いような気がします。

マッピング

シーケンシング後のリードをリファレンスゲノム対してアラインメントします。 Bowtie2、BWA、TopHat などのプログラムを利用します。 我々の場合は、 ゲノムリファレンスに対しては、TopHatでマッピングし、 Cufflinkで計数します。 トランスクリプトームリファレンスに対しては、Bowtie2でマッピングし、計数します。 計数はRのパッケージなども使えますが、出力データであるsamファイルからコマンド群を使って 容易に抽出できますので、学生の練習の意味で awk, grepなどのコマンド群で計数します。 最近は、マルチコア、大量メモリのサーバが使えるので、Bowtie2などのマッピングは数分で 終了します。また、シェルスクリプトで並列処理も簡単にできますので、多検体データも簡単に 処理できます。

TopHat(インストール)

いろいろな人が解説をウェブに上げていますが、まず以下を読みましょう。 http://ccb.jhu.edu/software/tophat/index.shtml インストール： 1. TopHatのインストールの前に、bowtie2 (or bowtie) とsamtoolsをインストールし、 以下のコマンドのパスを通します。 bowtie2 (or bowtie) bowtie2-build (or bowtie-build) bowtie2-inspect (or bowtie-inspect) samtools 2. Tophatをダウンロードし解凍後、パスを通します。 wget http://ccb.jhu.edu/software/tophat/downloads/tophat-2.0.11.Linux_x86_64.tar.gz tar zxvf tophat-2.0.11.Linux_x86_64.tar.gz 3. Cufflinksもダウンロードし解凍後、パスを通します。 yum install –y rpmlib でバイナリパッケージをインストール後 wget http://cufflinks.cbcb.umd.edu/downloads/cufflinks-2.1.1.Linux_x86_64.tar.gz tar zxvf cufflinks-2.1.1.Linux_x86_64.tar.gz

TopHat（続き）(インストール) 4. bowtie2のインストール　（１〜３より先に行う） wget http://sourceforge.net/projects/bowtie-bio/files/bowtie2/2.1.0/bowtie2-2.1.0-source.zip unzip bowtie2-2.1.0-source.zip bowtie2-2.1.0-source.zip make 5. Samtoolsのインストール（１〜３より先に行う） sudo yum –y ncurses sudo yum –y ncurses-devel wget http://sourceforge.net/projects/samtools/files/samtools/0.1.18/samtools-0.1.18.tar.bz2 tar jxvf samtools-0.1.18.tar.bz2 cd samtools-0.1.18 vi Makefile Makefileの以下の行を以下に示すように書き換えます。 original: LIBCURSES= -lcurses # -lXCurses changed: LIBCURSES= -lncurses # -lXCurses 　 その後 make 作成されたsamtools をパスを通します。

TopHat（使い方）

インデックス有りの場合のマッピング 1.  Bowtie2によるインデックス作成 bowtie2-build <リファレンスファイル>.fa <リファレンスファイル> 2. TopHat によるマッピング tophat -p 14 –G <GTFファイル> -o <出力ファイル> <インデックスファイル>　<入力ファイル> 3. cufflinksによる計数 cufflinks -p 14 –G <GTFファイル> -o <出力ファイル> <インデックスファイル>　<入力ファイル> cuffdiff, cuffmergeを使う方法などもあるが（以下のサイトなど参照)、 我々はcufflinksを使って出した計数値を、Rかコマンドをつかって マージし、変動比、t検定、マンホイットトニーU検定などを行っている。 http://cat.hackingisbelieving.org/lecture/biwako/NGS-R-Bioconductor-2nd.html http://crusade1096.web.fc2.com/katei.html#3-2 ここではイルミナの場合の方法のみ示したが、その他の種のデータについては以下を参照 http://cell-innovation.nig.ac.jp/wiki/tiki-index.php?page=TopHat

TopHat（続き）（使い方）

インデックス有りの場合のマッピング注意点 １、インデックスファイル作成は、ヒトなどの場合非常に時間がかかりますので、 以下のサイトなどからダウンロードしたほうが楽です。 http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml ２、GTFファイル（または、GFFファイル）の出来不出来が解析の正否を左右します。 以下などからきちんと動作するGTFファイルを入手します。 http://support.illumina.com/sequencing/sequencing_software/igenome.ilmn ３、GTFファイルがない場合は、ゲノム配列とGenbankファイルから、 Bioperlのライブラリ(bp_genbank2gff.pl)を使って自分で作成します。

Bowtie2によるマッピング

トランスクリプトームデータへのマッピング 1. bowtie2-buildによるインデックス作成 bowtie2-build <リファレンスファイル>.fa <リファレンスファイル> 2. bowtie2によるマッピング シングルエンドリード bowtie2 [options]* -x <インデックスファイル> -U <リードファイル> -S　＜出力ファイル＞ ペアエンドリード bowtie2 [options]* -x <インデックスファイル> -１<リード１> -2　<リード2> -S　＜出力ファイル＞

マッピングの問題点

リファレンスによりかなり結果が異なる。 例えば、 bowtieでトランスクリプトームリファレンスにマッピングした場合の変動比と tophat でゲノムトランスクリプトームリファレンスにマッピングした場合の変動比が異なるという ことをしばしば経験する。この場合、トランスクリプトームリファレンスにマッピングした場合の ほうが変動比が大きくなる傾向がある（いつもではない。あくまで傾向で、すべてそうという わけではない）。 特に生物種によっては、トランスクリプトームのリファレンスと、ゲノムのリファレンスへのマップ 状況が異なり、リードがゲノムのリファレンスへ90％以上マッピングされるのに、 トランスクリプトームのリファレンスへ50％強しかマッピングされないような生物種が少なからず 存在する。　 →　非モデル生物に関しては、トランスクリプトームのデータをもとに自分で独自にアノテーション ファイル（GFFファイル）を作り直すなどの対策をしたほうがいいかもしれない。

並列処理と逐次処理

スパコンやクラウドなどでは、OpenMPやMPIなどのしくみを 使って並列処理を実現する。 これらは、特殊なプログラミングを使ってメッセージ交換など を行う必要がある。（詳細は省略します。理研のサイトなどに 詳しい解説があります。）ただ、その環境を使える人は多くな いと思われる。 ただし、通常のマルチコアのサーバでも、シェルのコマンドを つかって逐次処理、並列処理が簡単に出来るので、適宜利用 するとデータ解析はかなり楽になるので、紹介する。 本ラボでは、並列処理で、マッピングは多検体（25検体とか）でも 数分で終了することが普通

理研のスパコン利用

理化学研究所HPCI講習会資料より

並列処理と逐次処理の実際例

並列処理 ＆の利用: 以下のように＆でコマンドを連結させることで５個のbowtie のプロセスを同時に 実施できます。 bowtie2 –p -x index1.fa –U test1.fastq –S out1.sam & Bowtie2 –p -x index2.fa –U test2.fastq –S out2.sam & Bowtie2 –p -x index3.fa –U test3.fastq –S out3.sam & Bowtie2 –p -x index4.fa –U test4.fastq –S out4.sam & Bowtie2 –p -x index5.fa –U test5.fastq –S out5.sam 逐次処理 ＆＆の利用:マッピングのあとのsamtoolsの連続処理や、tophat-cufflinks などのように連続した ルーチンワークが続くときは、＆＆を利用した逐次処理で実施できます。 samtools view -bS aln.sam > aln.bam && samtools sort aln.bam aln && samtools index aln.bam && samtools faidx ref.fa && samtools mpileup -uf ref.fa aln.bam | bcftools view -cg – tophat -p 14 –G <GTFファイル> -o <出力ファイル> <インデックスファイル>　<入力ファイル>　&& cufflinks -p 14 –G <GTFファイル> -o <出力ファイル> <インデックスファイル>　<入力ファイル> http://jehupc.exblog.jp/15729095/ など参照

Trinity

De novo RNA-Seq アセンブリ用ソフトウェア 1. インストール wget http://sourceforge.net/projects/trinityrnaseq/files/trinityrnaseq_r20140413p1.tar.gz zxvf trinityrnaseq_r20140413p1.tar.gz cd trinityrnaseq_r20140413p1 make 注意点：バージョンによってはJava のバージョンがあわず makeが通らないことがある。 そのときは、複数バージョンのJava をインストールして alternatives コマンドでバージョンを変更して コンパイルする。 2. 使い方 Trinity --seqType fq --JM 100G --left reads_1.fq --right reads_2.fq --CPU 6 注意点：最近コマンド名が変わったので、ウェブ上の古いマニュアルを見るときは注意。

De novo RNA-Seq の方法論

アセンブリ、アノテーション：　読んだリードをデノボアセンブリし、それにアノテーションをつける。 マッピング：　これをリファレンスにマッピングを行う。 ゲノムの部分配列など他の情報があれば、これにアノテーションをつけて、マッピングすることも 考えられる。 読んだリードをデノボアセンブリは、Trinity, Velvet-Oases がよく用いられる。 出力結果は、Fasta 形式である。 Fastaファイルは、シェルコマンドやRなどを用いて、平均リード長や、N50、リード長さの分布など を調べる。 また、コンティグをリファレンスにしてマッピングし、そのマッピング情報により、samtoolsを用いて カバー率、深度などを調べる。多型情報などにより、アセンブリエラーを見つけたり、 適当な配列編集ソフトで正確な配列に修正することも可能。

Velvet-Oases

De novo RNA-Seq アセンブリ用ソフトウェア Velvet のインストール: wget http://www.ebi.ac.uk/~zerbino/velvet/velvet_1.2.08.tgz tar zxvf velvet_1.2.08.tgz $ cd velvet_1.2.08 make 'MAXKMERLENGTH=101’ ./velveth ./velvetg パスを通しておく。 Oasesのインストール: wget http://www.ebi.ac.uk/~zerbino/oases/oases_0.2.08.tgz tar zxvf oases_0.2.08.tgz # cd oases_0.2.08 gmake 'VELVET_DIR=/path/to/velvet/velvet_0.2.08' 'MAXKMERLENGTH=101’ ./oases パスを通しておく。

Velvet-Oases(使い方)

ManualのFor Impatient People の項目は非常によく出来ているのでこれを参考にする。 velveth directory 21,23 data/reads.fa velvetg directory_21 -read_trkg yes oases directory_21 ls directory_21 velvetg directory_23 -read_trkg yes oases directory_23 velveth mergedAssembly 23 -long directory*/transcripts.fa velvetg mergedAssembly -read_trkg yes -conserveLong yes oases mergedAssembly -merge Or use the python script oases pipeline.py. python oases_pipeline.py -m 21 -M 23 data/reads.fa

ビューア　IGV

ブロードで開発されたIGV がよく使われています。 IGV インストール wget http://www.broadinstitute.org/igv/projects/downloads/IGV_2.2.5.zip unzup IGV_2.2.5.zip cd IGV_2.2.5 ./igv.sh パスは、igv.sh と igv.jarの２つを通す必要があります。 使い方 下記の方法で、インデックスを作成した後に、閲覧します。 samtools view -bS aln.sam > aln.bam && samtools sort aln.bam aln && samtools index aln.bam && samtools faidx ref.fa https://www.youtube.com/watch?v=5kkPnCV06dE

IGVの使用例

アノテーション BLAST, BLAST+がよく用いられる。 インストール方法： BLAST バイナリパッケージをダウンロードして解凍後, パスを通しておく. wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/LATEST/blast-2.2.26-x64-linux.tar.gz tar zxvf blast-2.2.26-x64-linux.tar.gz BLAST+ バイナリパッケージをダウンロードして解凍後, パスを通しておく. wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.2.28+-x64-linux.tar.gz tar zxvf ncbi-blast-2.2.28+-x64-linux.tar.gz 使い方：コマンドのみ blastall -p blastx –d Osativa_193_peptide.fa -i Consensus.fa -m 9 > Consensus_Osativa_193_peptide_BLASTXOUT.txt 以下のサイトなどを参考にしてください。出力結果をシェルコマンドやRを用いて集計します。 http://www.geocities.jp/ancientfishtree/LocalBlast_JI.html

パスウェイ解析、GO解析

GUIツールですので、リンク先を示すにとどめます。 DAVID：　パスウェイ解析、GO解析が簡単に出来るウェブツールです。 http://david.abcc.ncifcrf.gov/ 使い方は、GUIでそんなに難しくないので以下などを参考にしてください。 http://togotv.dbcls.jp/20090925.html BLAST2GO:　GO解析が簡単に出来ます。 http://blast2go.com/b2ghome GSEA: パスウェイ解析、GO解析を行います。 http://array.cell-innovator.com/?p=2030 GOなどは、自分でデータべースのテーブルをダウンロードしてデータベース化し、 クエリで得られたデータをフッシャーの正確確率検定などをやった方がうまく情報が引き出せる こともあるので、ツールに頼らず自力の解析を試みてみるのもよい。

R 統計解析環境で、データ解析には欠かせません。Rはバージョンアップが頻繁で、RedHat系 Linuxでは、バイナリパッケージの提供が遅いので、以下の方法で自分でコンパイルしています。 インストール方法（＊はバージョン番号が入ります。） wget http://cran.ism.ac.jp/src/base/R-3/R-*.tar.gz tar zxvf R-*.tar.gz mkdir R_* cd R-* sudo yum install readline readline-devel sudo yum install libXt libXt-devel sudo yum install libX11 libX11-devel sudo yum install cairo cairo-devel sudo yum install libjpeg libjpeg-devel sudo yum install libpng libpng-devel sudo yum install libdiff libdiff-devel sudo yum install texlive* sudo yum install texinfo wget http://genome.lab.tuat.ac.jp/~kazuoishii/files/inconsolata.sty ./configure --prefix=/home/genome/Packages/R/R_* sudo make sudo make install

R Rは Haskellや、Lispのような関数型言語で、 Lispの方言のSchemeのセマンテックをもとに、 ベル研究所で開発されたSをまねて作られています。 ベクトルの処理のしかたなどが、 Rubyににています。 なので、Rubyや、Lispを勉強するとRの理解が深まりますので、是非勉強してください。 意識して書くと、Lispそっくりのコードになります。 生物学的パッケージがたくさんそろっており、便利ですが、 現在のところ、マルチコアで動作することができない。メモリダンプを起こしやすく、 動作が遅いなど、実はビッグデータ処理には向いていません。 以下で、インタラクティブな使い方に慣れてきたら、出来るだけ、 関数やスクリプトを書いて、バッチ処理や並列処理で対応してください。 http://cse.naro.affrc.go.jp/takezawa/r-tips/r.html http://www.iu.a.u-tokyo.ac.jp/~kadota/r.html http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html 以下にバッチ処理の汚い例で、申し訳有りませんがご紹介しています。 http://www.ospn.jp/press/20130124no32-1-useit-oss.html

Rの使用例

●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●

●

●●●●●●●●●●

●

●

●

●

●

●●●●●●●●●●●●●●●●●●● ●●●●●●●

●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●

●●

●

●●●●

●

●

●

●●●●●●●●●

●

●

●

●●

●

●

●●●● ●

●●

●●

●

●●●●●●●●

●● ●

●

●●

●●●●●●●●●●●●

●●●

●●●●

●

●

●●●

●●●●●●●

● ●●

●●●

●

●● ●

●● ●

●

●●●

●●

●

●●●●

●

●●● ●●●

●

●

●

●●

●●

●

●●

●●●

●

●●

●

●●

●●

●●

●●●

●

●●●● ● ●●●●●

●

●●

●●●● ●●

●

●

●●●●●●

●

●●●●●●

●

●

●

●●●

● ●

●

●

●●●●●●●●

●●●

●

●

● ●●

●●

●●

●●●

●

●

●●●●●●●

●●

●●●●●●●●

●

●

●●●●●●●

●

●

●●

●●

●

●●●●

●

●

●

●●●

●

●

●●

●

●

●

●●

●●●●●●

●

●●●●●

●

●

●●● ● ●●

●●

●

● ●●●

●

●●●●●●●●●●●●●●●●●●●●

●●

●

●

●

●●

●●●●●●●●●

●

●●●

●

●

●

●●●●●

●

●

●

●●●●●

●

●

●

●

●

●●

●●●●

●

●●●●●

●●●●●●

●

●

●●●●

●●●

●

●●●

●

●

●●●

●

●

●

●

●●

●

●●●●●●

●

●

●●

●●●●

●

●

●

●●●●●

●

●

●

●

●●

●

●

●

●

●

●

●●

●

●

●● ●

●

●

●●●

●

●●●●

●

●●

●

●

●

●●

●

●●●●●●

●

●

●

●●●●

●

●●●●●●

●

●

●●

●●●

●

●

●

● ●

●●●

●

●●●

●

●

●

●

●●●● ●

●

●●●

●●●

●● ●

●

●●● ●●●●●●●●

●

●●●●●●●●●●●● ●●●●●

●

●●●

●●

●●●●●●●●

●●

●

●

●●●●●

●

●

●

●

●

●

●●

●●

●

●●●

●

●

●

●

●

●●

●●

●

●●●●

●

●●●

●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●

●●

●●●●

●

●

●●●●●●●●●●●●●

●

●

●

●

●●

●● ●

●

●●

●

●●

●●

●●●

●●

●●●●●

●●

●

●

●

●●●●

●

●

●●●●●●●●

●

●

●

●●

●●●●

●●●●●●●●●●●●● ●●● ●

●●

●●

●●

●

●

●

●

●●

●

●●

●

●●

●

●●●

●

●

●●●●

●●●●●● ●●●●

●●

●●●

●

●

●●

●

●●●●●●●

●●● ●

●

●●●●

●

●●●

●

●●●●●

●

●

●

●

●●

●

●●

●●

●●

● ●

●●●●●

●

●●●●●

●

●

●

●

●

●●●●

●●

● ●

●

●

●

●

●●

●

●●●

●

●

●●●●●

●●●●●●●

●●●●●● ●

●

●●

●●

●

●

●

●

●●

● ●

●●

●

●●

● ●

●●

●

●●

●

●●●

●

●●●●

●●●

●●●●

●●

●●

●●

●

●●●●●●●

●●●●●●●

●●

●

●●●●

●

●

●

●

●●

●

●

●●

●

●●

●

●

●●

●●●●

●

●

●

●

●●

●

●

●

●

●

●●●●●●●● ●●●

●●● ●●

●

●

●

●

●●●

●

● ●●●●●

●

●●●

●

●●

●●

●●●●●

●●

●●●●

●●

●

●●

●● ●●●●●

●

●●●

●

●●●●●●●●

●

●●

●● ●

●

●

●●●●●●

●

●●●●●●●

●

●

●

●

● ●●

●

●

●●

●●

●

●

●●

●●●

●●●●●●●●● ●●●

●●●

●●

●●

●●

●●

●●●

●

●

●●●● ●●●

●●

●

●●

●●●●●●●●

●●●

●●

●

● ●●

●● ●●●●

●

●

●

●●●●●●●●

●

●●

●●

●

●

●

●●●●●●●●●●●●●●●●●

●

●

●

●●

●

●●

●

●●

●

●

●●●●●●

●

●

●

●

●●●●●● ●●●

●

●

●

●●

●●●●●●●●●

●

●

●●

● ●●●●

●●●

●●●●●●●●

●

●●●●●●●

●

●

●●●●

●

●

●

●

●

●●

●●

●

●●

●

●

●●●●●●

●

●

●●

●

●

●●●●●●●●●

●

●

●●●●●●

●

●

●

●

●

●

●●

●

●

●

●●●

●

●

●

●

●

●

●●

●

●

●

●●●

●●●

●

●●●

●

●

●

●

●●●●●●●●

●

●

●

●

●●●●●●

●

●

●●

●

●●

●●●●●● ●●●●●●● ●

●

●

●●

●

●●

●●●●

●

●●●●●●●

●

●●●

●

●

●● ●●●

●

●●●

●●

●●●

● ●●

●

●

●●

●

●●●

●

●

●●●

●

●

●

●●●● ●●

●● ●●

●●

● ●

●

●

●●●●

●●●●●●

●●●●●●●●

●

●●●●●

●

●●

●●●

●

●

●●●

●

●●●

●

●

●

●

●

●●●●●●●●●●●●

● ●

●

●●●●●●

●●

●

●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●

●● ●●●●●

●

●●●●●●●

●●●

●

●

●

●●●

●

●●

●●●●● ●●

●

●

●●●

●

●

●

●●●● ●

●●●●●

●

● ●●●●

●

●●●

●●

●

●●●

●●●●●●● ●

●

●●●●●●●●●●● ●

●

●

●●

●●

●

●

●

●

●

●●

●

●

●

●●● ●●

●●

●●

●

●●

●

●

●●●●●●●

●

●

●

●

●●●

●● ●

●

●

●●●●●●●●●

●

●●●

●

●●

●●●●●

●●●

●

●

●

●●●

●

●●

●●

●

●

●●●●●

●

●

●

●●

●

●●●

● ● ●

●

●●

●

●●●

●●

●

●

●●

●● ● ●●●

●

●●●●●

●

●

●

●

●●

●

●●

●●●●●●●

●●

●●

●●

●

●●●●●

●

●

●●

●

●●●● ●●

●

●

●

●●

●●

●●●●●●●●●●●●●●●

●●

●●●●●

●●●

●

●

●●

●●●

●●

●

●●

●

●

●

●●●

●●●

●●●●●●●●●●

●

●

●●● ●

●

●●

●●●

●

●

●●●●●

●●

●●●●●

●●●●●●●●●●●●●

●

●

●

●

●

●

●

●

●●●●●●●●

●

●

●●●●●

●

●●●●●●●●●● ●●●●●●●●●●●●●

●

●●●

●

●●

●●

●

●

●

●●

●●●●●●

●

●●●●

●●● ●●

●●

●

●●●●●●●●

●●●●●●●●●●●●●●●

●●●●●●●●● ●●● ●●●

● ●●●●

●●●●●●● ●●

●

●●●

●●

●●●●●

●

●

●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●

●

●

●●●

●

●

●●●

●

●

●

●●●

●●●●●●●●●●●●●●●●

● ●●

●

●●●●●●●●●

●●●●●●●●●●●●●

●●

●●

●

●●

●●●

●●

●●●

●

●●

●

●●● ●●●●●●●●●●●●●●●●

●●

● ●●● ●●

●●

●●●●●

●

●●●●●●

●●●●●●● ●

●

●●

●●●

●●●●●●●●●●●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●

●●

●

●●●

●

●●●

●

●●●●●●

●●●●

●●● ●●

●

●●●●●●●●●●●●●

●

●●●●●● ●

●

●

●●●●

●●●

●●

●

●●

●●●●

●

●

●●●

●

● ●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●● ●

●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●

●

●●●

●

●

●

●●●●

●●●

●●●

●●●

●

●

●●

●●●●●●●●●●●●●●

● ●

●●●●●●●

●

●●●●●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

● ●●●●●

●

●●

●●●●●●●●●●●●●●●● ●●

●● ●

●

●●●●

●●●●

●●●●●●●●●●●●

●●●●●●●● ●●●●

●●

●●●●●●●●●●●●

●

●●●●●●●●●

●

●●●●●

●

●●●●●●●

●●●●●

●●

●

●

●●●

●●●

●●

●●●●

●

●● ●●●●●

●

●●●●

●

●●●

●●

●

●

●

●●●●●

●●

●● ●●●●

●●

●●●●●●●

●●

●●

●

●●

●

●●●●●

●●

●●

●

●●

●●●●●●

● ●

●●●●●

●

●●●

●

●●

●

●

●●

●●

●●●

●

●

●●●● ●●● ●●

●●

●●●●●●●●●

●

●●●●●●●

●

●

●

●●●●●●●

●

●

●

●●●

●●●●●

●

●●●●●

●●

●●●●

●

●

●●

●

●●●●●●●●●

●

●●

●●

●●●●●●●

●

●

●●●●● ●●●●●● ●

●

●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●

●●

●●●●

●●●●●●●●●

●●● ●● ●●●

●●●

●

●●●

●

●●●●●● ●●

●

●●●●●●●●●●●●●

●

●

●

●

●

● ●●●●●●●●●●●●

●

●●●●●●●●●●●●

●

●●●●●

●●●●●

●

●●●● ●●

●●●

●●

●●●●●●●●●●●

●●●●●●●●●●●

●

●●●●●●●

●●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●● ●● ●●●●●●●●●●●

●

● ●●●●●●●●●●

● ●●

●

●●●●

●●

●●

●●●●●●

●

●●●●

●●●●●●

●

●

●●

●

●

●

●●●●●

●

●●●●●●●●●●●●●●●

●●●

●●

●

●

●●● ● ●

●

●●●●●

●

●

●

●●●●

●

●●

●

● ●

●●●●

●

●

●

●●●●●●●●●●●●●●●●●

●●●●●

●

●●● ●

●●

●●

● ●

●

●

● ●●

●

●●●●●

●●

●

●●●●●●●

●

●●●

●●

●

●

●●

●●●●

●

●●

●●●●●●●●

●

●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●

●

●●●

●●

●●●●●●●●●●●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●

●

●

●●●

●

●●●●●●●●

●

●

●

●●●●●●●●●●

●

●

●

●●

●

●●●●

●●●●

●●●

●●●●●●●●●●●●●● ●●●

●

●●●●●●

●●●

●●

●●

●●●●●●●●

●●●●●●●●●●

● ●

●●●●

●

●●●●●●●●● ●

●●

●●

●●●●●●●●

●●

●● ●

●●

●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●● ●●

●●●●●●●●●●●●●●●●●●●●

●

●●

●●●

●

●●●●●●●

●

●●●●

●

●●●●●●●

●

●

●●●

●

●

●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●

●

●●

● ●●●●

●

●●●●●●●●●

●●●●●●

●

● ●●●●●●

●●

●●

●

● ●● ●●●●

●

●●

●●

●●●

●

●● ●

●●

●●●●●●

●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●● ●

●●●●●●●●

●

●●●

●

●

●●●●● ●●

●

●

●

●

●

●

●●●

●

●

●●●

●

●●●●●●●●●

● ●●●●●

●

●

● ●●●●●●●●●●●

●

●●●●●●

●

●●

●●●●

●●

●

●●●●●●●●●

●●

●●●●●●●●●●

●●

●● ●●

●●

●●●●●●●●● ●● ●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●

●

●●

●

●

●●●●●

●●●●

●

●

●

●

●

●●●●●●●●●

●●

●

● ● ●●●

●

●●●● ●●●

●●●●●●●●●●●●●●●●●●●●●

●●

●●●●

● ●●

●●●●●●●●

●●

●

●●●

●●

●●●●

●

●

●●●●●

●

●●●●●

●

●●●

●

●●●

●

●● ●● ●

●

●●●●●●●

●

●●

●●●●

●

●

●

●

●

●

●●

●●

●●●

●●●●●●●●

●●

●

●●

●

●●●●●●●●●

●

●

●●

●●

●●●

●●

●●

●

●●●

● ●●

●

●●●

●

●●●●●●

●

●

●

●●●

●●●●●●●●●●

●

●●●

●●●●

●

●●●●●●●●●

●●●●●

●●

●●

●●

●

●

●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●

●

●●●●●●●

● ●

●●

●

●

●

●●●●

●

●●●

●

●

●

●

●●

●

●●●●

●

●●

●

●●

●●●●●●●●

● ●●●●●

●

●

●● ●

●

●●

●

●

● ●●●●

●

●●● ●●

●●●●

●●●●● ●●●●

●

●

●●

●●

●

●

●●●

●

●

●

●

●●●●●●●●●●●●●●

●

●

●●

●

●●

●●

●●●●●

● ●

●

●

●●

●●

●

●

● ●

● ●

●

●

●●

●

●

●

●●●●● ●●●

●●●

●●

●●

●●

●

●●

●

●

●●

●●

●

●

●●

●

●● ●●

●

●●●●●●●

●

●●

●

●●●●●●●●

●

●● ●●●●

●

●

●●

●●

●●●●

●

● ●●●●●●

●●

●●

●

●

●●●●●●●● ●●●

●

●

●●

●●

●

●

●

●

●

●

●●

●

●

●●●●

●●

●

●●●●●

●●

●

●●●●●●●●●●●●● ●

●●

●

●●●

●

●

●●●●●●

●

●●●●

●

●

●●●

●●

●●●●●

●●

●●●●●●●●●

●

●●●

●

●

●

●●●●●●●●●

●

●

●●●

●

●●●●●●●●

●

●●

●

●

●●●●

●●●●●●

●

●●

●●●●●●●

●

●

●

●●●●

●

●

●●●●●●●●

●

●●

●

●●●●

●

●

●

●●

●

●●●●●●●●●

●●●●●●

●

●

●

●

●●●●

●●

●●●●●●●

●

●

●●

●●●

●

●●●●

●

●●

●

●●●

●●●●●●●●●●●●●●●●

●

●

●●●●●

●

●●●●●●●●●●

●●●

●● ●

●●●●

●

●●●●●●●●●●

● ●●●

●●●●

●●●●

●●

●●●●●●●●●●●●●

●●

●●●●

●

●●●●●●

●

●

●

●●●

●●●●●●

●●

●●●●●

●●●

●

●

●

●

●

●

●●

●

●

●

●●●●●●●●

●●

●●

● ●●●

●

●

●●

●●

●●●●●●

● ●●●

●●●

●

●

●

●

●

●

●

●

●

●●●●

●

●

●●

●●●●● ●

●●●●

●

●

●

●●

●●

●

●●●●

●●●●●●●●

●

●●●●●

●

●

●

●

●●

●●●●●●●●

●

●

●●●●●

●● ●●●

●

●

●●●● ●●●

●●●

●

●

●●

●●

●

●

●●

●●

●●

●

●●●

●

●●●●●

●

●●●●●●●●●●●●●●

●

●

●●

●●

●

●●

●

●

●●

●

●

●●●

●

●

● ● ●●

● ● ●●

●●●

●

●●●●

●

●

●

●●

●●●

●● ●●●

●

● ●●●

●●●●●

●●●●●

●●

●

●●

●●●

●

●●●

●

●

●●●●

●●●

●

●●●● ●●

●

●

●●●●

●●

● ●●

●

●●●●●

●●

●●●

●●●● ●●

●●● ●●

●

●

●●●

●● ●●

●●●●

●●

●

●●●●

●●

● ●●●

●●●●●●●

●

●

●●

●●

●●

●

●

●

●

●

●●

●

●

●

●●●●●●●●●●

●

●●●

●

●●

●

●

●

●

●

●●

●

●

●

●●●●●●●●●●●●●●● ●●

●●●

●

●●●●●●

●

●●

●

●●●

●

●●●●●●

●

●

●

●

●●

●

●

●

●●●●●

●● ●

●●

●●●●●

●●

●

●

●

●●

●●●●●

●●

● ●

●●●●

● ● ●●

●●

●

●●●

●●●

●

●●

●●

●● ●●

●

●

●● ●●

●

●

●●

● ●●●●●●●●●●●

●●●

●

●●●● ●●●●●

●●

●

●

●●

●

●●●●●●

●

●●

●●●●

●●●●●●●●

●●

●

●

●

●

●●●●●●●●●

●

●

●

●●●●

●●●●

●●●●●●● ●●●●●●●●● ●

●

● ●●●●●●

●

●

●

●●

●

●

●●

●

●

●

●

●●●●

●

●●

●

●●

●● ●● ●●

●

●●

●●

●●

●

●

●

●

●●●●●●

● ●

●

●

●

●●●

●●●

●●●●●● ●

●

●●

●

●●

●●

●

●●●●●

●

●●

●

●

●

●

●

●

●

●●●●●

●

●

●

●●●●●●●●● ●●

●

●●

●

●●●●●

●●●●

●

●●● ●

●●

●●●

●●

●●●●●●

●●●

●

●●● ● ●●●●

●

●

●

●

●● ●●

●

●●

●

●

●●

●●●●●●●

●●

●●●●

●

●

●

●●

●●●●●●

●

●●●

●●

●

●

●

●●

●

●●●

●

●●●●●●●●

●●●●

●●

●●●●●●●●●●● ●

●

●●

●●

●●●●● ●●● ● ●●●● ●

●

●●

●

●●

●●●●

●●

●

●●●●●●●

●

●

●●

●

●

●●

●●●●●

●●●● ●●

●

●

●●

●

●●

●

●

● ●●●●

●

●●●●●●●●●●

●●

●●●

●● ●●●

●

●

●●●●

●

●

●●●●● ●●●●

●

●●●

●

●●●● ●

●

●

●

●

●●●●

●●

● ●●

●

●●●●●

●● ●

●

●

●

●

●●●

●

●

●

●●

●●

●

●

●

●●●

●●

●

●●●

●

●

●

●

●

●●

●

●

●●● ●●

●●

●●●

● ●

●●

●

●●●●●●●●

●●●●

●

●

●●●●● ●●●●●●●

●●●

●●

●

●

●● ●●

●

●●●

●

●●●

●

●●●●●

●●●●

●●

●

● ●

●

●

●

●

●

●●

●

●

●●

●

●

●

●●

●

●

●

●

●●

●●●●

●●

●● ●●

●●●

●●●

●●●

●●●● ●●●

●●●

●

●

●

●●●●●●●●

●●●

●●●●

●

●

●●●●

●

●

●

●● ●●●

● ● ●

●●●

● ●

●

●

●

●

●

●●●

●

●●● ●●●●●●●●●●

●●

●●●

●

●

●●●●●●●●●●●

●●

●

●●●●●

●●

●●●●

●●

●

●

●

●●●●

●●

●● ●●●●●●●

●

●●

●●●●●●●●

●

●

●●

●

●

●

●●

●

●●●●●

●

●

●●●●●●

●

●

●

●●●●●●●

●

●●● ●●●●●

●●

●●●●

●

●●●

●

●

●● ●

●

●●●● ● ●●

●

●

●

●●● ●●

●

●

●

●

●

●

●

●

●

●●

●●

●● ●●

●●

●

●●

●

●

●

●

●●●●●

●

●●●●

●

●

●●●●

●●

●

●

●●●

●●

●

●

●

●

●

●●●●●●●●

●

●

●●● ●

●●●●●●●● ●●●●●●

●

●

●●

●●

●

●●

●

●

●●●●

●

●

●●●●●●●●●●●●

●●●●

●●

●●●●●●●●

●

●●●●●

●

●●●

●

●

●

●●

●●

●●●●●●

●●

●

●●●●

●● ●●●●

●

●

●

●●●●●●

●●●

●● ●

●

●●● ●●●

●

●●

●●

● ●

●● ●●

●●

●●●●

●

●●●●

●

●●

●●●●●●●

●●●

●●●●●●

●

●

● ●

●●

●●

●

●●

●●●●●●

●●●

●●●●

●

●●●●●●●●●

●

●●●●●

●●

●●

●

●●

●●●●●●●●●●

●

●●●●●

●●●●●

●●

●

●

●●●●●●●●●

●

●●

●

● ●

●●● ●●●●

●

●

●

●●

●●●

●

●

●●●●●●●●●●●●●●

● ●●●●

●

●●

●

●

●●●

●●

●●●●●

●● ●

●

●

●

●●

●●

●

●

●

●

●

●●

●●

●●

●

●

● ●●

●●●●

●

●

● ●●

●

●

●

●●●●●●

●

● ●●

●

●

●

●

●

●

●

● ●●●

●

●●

●

●●●●●●

●●●

●● ●●●

●●

●

●

●

●● ●

●●●●

●

●

●●

●

●

●

●

●

●

●●

●

●●

●●● ●

●● ●

●

●

●●●●

●

●

●●●

●

●

●●●

●

●

● ●

● ●●

●

●●

● ●●●

●●●●●●●●●●●●●●

●

●●

●

●

●●

●

●●●●●●●● ●

●

●

●●

●

●

●

●●●

●●●

●

●

●

●●● ●

●●

●

●●

●

●

●

●

●●

●

●●●

●●●

●

●●●●●●●●●

●●●●●

●

●

●● ●

●●●

●

●

●●

●●

●

●

●●●●●

●●●●● ●

●

●●●●●●

●●

●

●●●●●●●●●●●

●

●●●●●●

●

●● ●●●●●●

●●

●

●

●

●

●

●

● ●

●

●

●

●

●

●

●●

●●

●●●●

●

● ●●

●●●

●●●

●●●●●

●

●●●

●

●

●●●●

●

●

●

●

●●●●

●●●●●

●●

●

●●●●●

●

● ●

●

● ●●

●

●●

●●●●

●

●●●●●●●●

●

●

●

●

●●

●●

●●

●●●

●

●

●

●●●●●●● ●

● ●●●●●

●

●●●●

●

●●

●

●

●

●●

●

●●●

●●● ●●

●●

●

● ● ●●●●●●

●

● ●●●

●●

●●●

●●●

●●●●●●● ●●

●● ●●

●●●●

●

●●●

●●

●

●

●

●

●

●

● ●

●

●

●

●●●●

●

●●●

●

●

● ●

●

●●●●●

●

●●

●

●●●●

●

●●

●

●

●●

●●●

●●●●

●

●●●●●●

●●

● ●●●●

●

●●

●

●●

●

●

●●●●

●

●

●●

●

●●●●

●●●

● ●

●●

● ●

●

●●●● ●

●

●

●

●●●●●

●●●●●

●●

●●

●●

●●

●

●●

●●●

●●●●

●

●● ●●●

●

●

●

●● ●

●

●●●

●

●●●●●●

●

●

●●

●

●

●

● ●●●●

●

●

●

●●●●●●●●●●

●

●●

●

●

●

●●●●

●

●

●

●

●●●

●

●

●●

●● ●●

●

●●

●

●

●●

●●●●

●●

●●

●●●

●

●●●●

●

●

●

●

●

● ●●●

●●● ●

● ●●●●●

●●●

●●

●●

● ●●●

●

●

●

●●●●

●

●

●●●●●

●●●●

●

●

●●

●●

●

●●●

●●

●

●●

●

●●

●●●●●●●●●●●●●

●●●●

●●

●

●

●

●●

●

●

●●

●

●

●

●●●●

●●

●●

●

●

●

●●●●

●

●●● ●

●

●●●

●

●

●●

●●

●

●

●●● ●●

●

●

●●●●●●

●

● ●●

●●●●●

●

●

●●●●●● ●

●

●

●●●●

●

●

●●

●●

●

● ●

●

●●●

●

●●●●●●●●

●

●●●

●

●●

●●

●●●

● ●●

●

●●●

●

● ●●●

●

●●

●● ●

●●●●●●●●

●●

●

●●●

●●●●●●●●●●●

●●

● ●●

●

●

●

●

●

●

●

●

●

●

●●●●●●●●

●● ●

●●

●● ●

●● ●

●

●●●●

●

● ●

●

●

●●

●

●●●

●●●

● ●

●

●

●

●

●●

●●

●●●

●●

●

●●

●●●

●●

●●

●●

●●

●●

●●●

●●●●●

●●●●

●●

●●

●

●●●●

●●●●●

●

●

●

●●●

●

●●●

●

● ●● ●

●

●●●●

●●

●

●

●●

●

●●●●●●●●● ●●

●●●

●

●

●

●

●

●●●

●

●

●

●●

●●

●●●

●

●●●●

●

●

●●

●●

●

●●

●●●

●●

●

●

●●

●●

●●

●

●●●

●

●

●●●

●●●●●●● ●●

●

●●

●●●●

●

●

●●

●

●

●●

●

●

●●●●●●●●●●●

●

●●●●

●●●●

●

●

●●●●

●

●

●●●

●●

●●●●●●

●

●●●●●●

●

●●●

●●●

●

●

●

●●

●

●●●●●

●

●●●●

●●●

● ●

●

●●

●

●

●

●●● ●

●●

●●●●

●

●

●●

●

●

●

●

●

●

●

●

●●●● ●

●

●

●●

●

●●● ●

●

●●●

●

●

●●

●

● ●

● ●

●●●

●

●●

●

●●●

●

●●●

●

●●

●

●●●

●

●●

●

●

●●●●

●

●

●

●

●●●●

●●

●

●

●

●●●●

●●

●●

●●

●

● ●

●

●

●

● ●

●●●

●

●

●

●●● ●●

●

●

●

●

●

●

●

●

●●●

●●●

●

●●

●

●●

●●●●

●

●

●●●●●●● ●

●

●●

●

●●

●●●● ●

●●

●

●

●

●

●

●

●●●●

●●●

●

●

●●●

●●

●●

●●●●● ●

●

●

●

●●

●●●●●●●● ●●

●●

●

●●

●●

●●

●

●

●●

●●●●

●●

●

●

●

●

●●●●●●●

●

●●●●●●●●●

●

●●●●●

●

●●

●

●

●●●●

●

●

●

●

●

●

●

●●●

● ●

●●●●●

●

●

●

●

●

●●

●●

●●●●

●

●● ●●●

●

●

●

●●●

●

●●●

●

●

●●●●●

●●● ●●●

●●

●

●

●●●●●●●● ●

●●

●●●●

●

●●

●●

●

●●●

●●

●●●

●

●●

●

●

●●

●●●●●●●

● ●●●

●

●

●

●

●

●●●●

●

●●●●●●●●●●●●●●●●●●

●

●●

●●●●●

●

●●●●●●●●●

●●

●

●●

●

●

●

● ●●●●●●

●

●●●●●●●

●

●●●●●

● ●

●

●●●●●●●

●

●

●●● ●● ●● ●● ●

●●●

●

●●

●

●● ●

●

●●

●●● ●●● ●●●

●

●

●●●●

●

●

●●

●

●

●

●●

●●●●

●

●

●

●●

● ● ●●

●● ●

●●●●

●●

●

●

●

●

●●

●●

●●

● ●

●

●

●

●

●

●

●●

●

●●

●

●●

●

●●

●●

●

●●

●

●●●

●●

●●

●

●

●● ●●●●●●●●

●●●●●●●●●

●●●

●●●●

● ●●●●● ●

●

●●

●●●●

●

●●

●●●

●

●

●

●● ●

●●●●●●

●

●

●●

●

●

●

●●●

●●

●

●

●

●●●●●●●●●●

●

●●

●

●

●

●●●

●●●●●

●

● ●

●●●

●

●

●●

●●●●●

●●

●●●

●

●

●●●●

●●●

●●●●●

●

●●●●●●

●

●

●●

●●

●●

●

●●●●●●●

●

●●

● ●●

●

●

●●●● ●●

●

●

●

●

●●

●

●●●●

●●●

●

●●●

●●●●

●

●

●

●

●●●

●

●●●●●

●

●●●●●

●

●

●

●

●●

●

●● ●● ●●

●●● ●

●●

●

●●●●●●●●●

●●

●● ●●

●

●●●●●

●●

●●● ●●

●

●●

●

●

●

●●●●●●●●●●●●●●●●

●●

●

●

●●

●●●●●

●●

●●●●●●

●

●

●●●●● ●

●●

●●●

●

●● ●●

●

●●

●

●●●●●●

●● ●

●

●●●●●●●●●●●

●●● ●

●● ●●

●●●

●

● ● ●

●

● ●

●

●●

●

●●●●

●●

●●

●●●

●●

●

●

●

●

●

●

●

●

●

●

●●

●●

●●●●●●

●

●●●●●●

●

●

●●●

●●●●●●●●●●●

●

●●●●●●●●

●

●

●

●●●●

●●●

●●

●

● ●

● ●

●●●

● ●

●

●

●●●

● ●●●●● ●●●

●●●●

● ●

●●

●●●●

●●●●●

●

●

●

●●●●

●●

●●●● ●

●●●

●●●●●

●

●●●●●●

●●●●

●

●●

●

●

●

●●●●●●●

●

●●●

●

●●

●

●

●●●

●●●●

●

●●●

●

● ●●●●●●●●●●

●

●

●●●●●

●●

●●●

●●

● ●●

●

●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●

●●

●

●●

●

●

●

●

●

●●

●

●

●

●●●●●

●

●●●

●

●●

●●

●

●●

●●●●

●●●

●

●

●●

●●

●

●●●●●●●

● ●●

●●●

●●●

●●●●

●

●●●●

●●

●●

●

●

●●

● ●

●

●

●●

●

● ●●

●

●●

●

●

● ●●● ●●●●

●●

●

●

●

●

●●●

●

●●●●●●●●● ●

●

●●

●

●●

●●●● ●●

●

●●●●●●●●●●

●

●

●

●●

●

●●

●●●

●●

●

●

●●

●●●●●●● ●

●

●●

●●●●●

●●●●

●●●

●

●

●●●

●

●

●

●●

●

●

●●

●●●

●

●

●

●●●● ●

●●

● ●●●●

● ●●

●●

●

●

●

●●●

●●●

●●

●

●

●

●●●●

●

●●

●

●●●

●

●●● ● ●●●

●●

●●●

●

●

●●

●●●

●

●● ●

●●●

●●●●●

●

●●

●

●

●

●●●

●

● ●

●●●

●

●●●●

●

●

●

●●●

●

●

●●●

●●

●●

●

●●

●● ●

●

●●

●●●

●●

●

●

●

●●

●

●●●

●

● ●●●●●●

●●

●

●

●●

●

●

●

●

●

●

●

●

●●● ●

●●●

●●●●●●

●●●●●●●●●●●

●●

●●

●●●

●

●

●●●●

●●●●

●●

● ●●

●●●●●●

●●

●●●●●

●●

●

●●●

●●

●

●

●●●●

●

●●

●

●●●●●●●●●●

●

●●

●

●●

●

●●

●●●

●

●

●●●

●

●●●

● ●● ●● ● ●●

●●

●●

●●●●●●

●

●●

●

●●

●●●●●

●●●

●

●

●●●●●●●

●

●

●

●●

●●

●

●●●

●●● ●

●●●●

●●●

●● ●●● ●●

●●●●●●

●●●●●●●●●●●●●●

●●●

●

●●●

●●● ●

●●

●

●

●● ●●

●

●●●

●

●●●●

●

●●●

● ●

●

●

●●●●●●●●●●●●●●

●

●

●

●●●

●●●

●

●

●●

●

●●● ●●●●●●●●●●●●

●

●

●●

●

●

●●●

●

●●

●

●

●

●

●

●

●●●

●

●●

●●

●

●

●

●

● ●

●●●●●

●●

●

●

●

●

●

●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●

●

●

●

● ●

●

●●●●●●●●●●●●●●●●●●

●

●

●●

●●

● ●●●

●●

●

●●

●

●

●

●●●

●

●●●

●

●

●●●

●

●

●

●●● ●●●●●●●●●●●●●●●●●●●●●●●●

●●● ●

●●

●● ●●

●

●

● ●●●●●●●●

●●●●●●●●●

●

●●●●●

●●

●

●

● ●●

●

●●● ●

●

●

●● ●

●●●●

●

●●●●●

●

●●●●●●●●●●●●● ●●

●

●

●●●●●

●

●●●●●

●●

●

●

●●

●●

●●●

●●

●

● ●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●

●

●●●●

●●

● ●●●●●●●●

●

●●

●

●

●

●

●

●●●

●

●

●●●●●●●●●●●

●

●●

●●

●

●

●●

●

●●●●●●

●

●●●●●

●

●●

●

●●●●●●●●●

●

●●●●●

●

●● ●●

●

●

●

●

●

●●

●●● ●●

●

●●●●● ● ●●●● ●●●

●

●

●●●●●●●●●

●●

●

●●●

●

●

●●●

●

●●

●

●●●●●●● ●● ●●●●●

●●●●●●●●●

●●

●

● ●

● ●

●

●●●●●●●

●●

●

●●●●●●●●●●●●

●●●●●●●●●●●●●●

●●●●

●

●●

●

●

●●●

●

●●●

●●

●

●●●●

●

●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●

●●

●●

●

●●

●●●●●●●●

●

●●●●●●●●●

●●●●●●●●

●●

●

●

●●

●

●

●●

●●●●●●●●●●●●●●

●●●●●●●●●●

●●

●●●●●● ●●●●●●

●●●●●

●●●

●

●

●●●

●●●●●●●●

●

●●

●

●

●●● ●●

●

●●● ●●●●●●●●●●●●

●

●●●●●●●●●●●

●

●

●

●

●

●

●●●●●

●

●●●

●●

●

●●

●●

●

●●●●

●●

●

●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●● ●

●

●

●● ●

● ●●●●

●●●●

●

●●● ●●●●●

●

●●●●●●●●●

●●

●● ● ●

●

●●

●●

●

●

●●●●●●●●●

●

●

●●

●

●

●

●

●●●

●

●●●●●●●●●●●

●

●

●

●●●

●●●●●●●●●●●●●●●●●●●● ●●●

●

●● ●●

●

●●●

●●●●

●

●●●●

●●

●

●●

●

●●●

●

●●

●●●

●

●●●

●●●●●●●

●●

●●●●●●●● ●●●●

●

●

●●

●●●●●●●●

●

●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●●●●

●●

●●●●●●●●

●

●●●●●●●●●●●●●

●

●●●

●●

●●●●●●●●●●

●

●●●●●

●●●●●

●

●

●●●●●●● ●

●●●●●●●●●●●●●●

●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●

●

●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●

●●

●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●●

●

●●●

●● ●

●● ●●

●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●

●

●

●●

●● ●●●●

●●●

●●

●

●●●●

●

●●

●

●

●

●

●●

●

●

●●●

●

●

●●●●●●●●●

●

●

●

●●●

●

●●

●

●

●●●●● ●● ●●●●●●●●●●

●●●●●●●● ●●●●●●

●● ●●● ●

●●

●●

●

●●●

●●●●●●●●●●●●

● ●●

●●

●●

●

●●●●●●●

●●●●●●

●

●

●

●

●●●●●●●

●●

●

●●●●●●● ●●

● ●

●●●●●●

●

●

●●●

●●●●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●

●●●●●●

●

●●●●●●●●

●

● ●●●●●●●●●●●●●●●●●●●●●●●

●

●●

● ●

●●●●●●●

●

● ●●●

●

●

●

●

●

●●●●

●

●

●

●●●●

●

●●

●●

●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●

●●

●●●

●

●●●

●●●●●●●

●●●

●

●

●●

●

●

●

●

●●●●●●●●●

●

●●●●●●●●

●●

●●●●

●

●●●●●●

●

●●●●●● ●●●● ●

●

●●

●●

●●●●●●●●

●

●●

●●●●●

●

●●●●

●●

●●●●● ●

●

●●●●●

●

●●●●

●●

●●●

●●

●●

●

●

●

●

●●

●

●●●●●●●

● ●

●●●●● ●●●●●●●●●●

●

●

●●

●

●●

●●

●

●

●●

●●

●

●●●●

●

●

●

●● ●●●

●●●●

●

●●

●

●●●●

●

●●

●

●●

● ●

●●

●

●●●● ●

●●

●●

●●●●● ●

●

●

● ●

●

● ●

●●●

●●

● ●

●●●

●●●

● ●

●●

●

●●●●

●●●●●

●●

●●●

●

●●●

●

●●

●

●●●

●

●

●●●●●●●●

●

●●● ●●

●

●

●

●

●

●●●●

●

●●●●

●●●●●

●

●

●●●

●●●●

●

●●●●●●●

●●

●

●●

●

●●●●●●

●

●●

●●●

●●

●

●

●●

●●

●●●● ●● ●●

●

●●

●●●●●

●

●

●● ● ●●

●

●

●●

●●●●●

●

●●●●

●●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●

●● ● ●●

●

●● ●●

●

●

●

● ●●●●

●●

●●

●●

●

●

●●

●

●●●●●●●● ●●

● ●

●

●

● ●●●●●●

●

●●

●

●●●● ●

●

●●

●

●

●●●●●●

●

●

●●● ●●●●● ●

●

●●

●●●

●

●

●●●●●●●●●●●

●●

●

●

●●●

●●●

●●●●

●●

●

●●●

●

●● ●●

●●

●●

●●

●●●●●

●

●●

●●

●

●●●●●●●

●

●

●●●●●

●

●

●●

● ●

●●

●●

●●●

●

●

●●●

●

●

●

●

●●●

●

●●●

●

●●

●●●●

●●●●

●

●●

●●●●

●

●

●●●●● ● ●●●●

●●

●●

●●●

●

●

●

●●●●●●●●●

●●

●

●●●

●●●

●

● ●●

●●●●

●

●

●●●●●●●

●

●●

●●●

●

●●●

●●

●

●●●●●●●●●● ●

●

●

●

●

●●

●

●

●●●● ●

●● ●● ●

●●●

● ●

●

●

●●●

●

●●

●

●

●

●

●●●●●

●●

●

●●

●

●

●●●

●

●

●

●

●

●●●

●

●

●●●●●●●●

●

●●●●

●●●●●●●●●●●

●

●●●

●●●●

●

●●●●●●●●●●●

●

●●

●●

●●●●●●●●●

●

●●

●●●

●●

●●

●●

●●●●●●●●●

●

●

●●

●●●●●●

●●●●

●●●●

●

●●●●●

●●●

●

●

●

●

●

●●●●●

●●

●●●

●

●

●●

●●

●●

●●●

●

●●●●

●●●●

●

●●●●●●●●

●●

●●●●●●●●●

●

●●●●●●

●

●● ● ●

●

●●

●

●●

●●

●●

●●

●●●

●

●

●

●●●●●●

●●●●

●●●

●●●

●

●●●●

●●

●● ●●

●

●●●

●

●

●

●

●●

●

●

●

●●●●●●●●

●

●●●

●●

●

●

●

●

●● ●●

●

●●●●●●●●●●

●

●●

●● ●

●●

●●

●●

●●●

●●●●

●●

●

●

● ●● ●●●●●●

●

●

●●●●

●●

●

●●●●

●●●●

●

●

●●● ● ●●

●●

● ●●●● ●

●

● ●● ●●●●●●

●●●

●●●●●●

●●

●●●●●

●●●●

●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●●●●●●●●

●

●●

●●

●

●●

●

●

●●

●

●

●

●●●●●●●●●●●●

●

●● ●●●●●

●●●●

●

● ●●

●

●●●●

●●

●

●

●●

●

●

●●●●●●●

●● ●

●●● ●

●●● ●●●●●●

●

●

●● ●●●●

●

●●

●●●●●●●●●

●●●

●

●●

●

●

●

●●●●●●●

●

●●●●●●

●

●●●●

●●● ● ●●

● ●●●●●●●●● ●

●●●

●●

●

●●

●

●

●

●●● ●●

●●

●●●●

●●

● ●●

●

●●

●●●

●●

●●●●●●●●

●

●●●

●

●

●●●●

●●

●

●

● ●

●

●●

●

●●

●●

●●●●

●

●●●

●●●

●

●

●

●

●

●●

●

●●●

●●●●●

●

●●

●

●●●●

●

●●

●

●●●

●

●●

●●●

●●●

●

●

●●

●●

●●●

●

●

●

●●

●●

●

●●

●

●●●●

●●

●●●

●

●●●

●

●● ●

●●

●●●●●●●

●

●●●

●

●

●

●

●

●

●

●

●

●●●

●

●●●

●

●●●●●

●

●●

●●

●●●●

●●

●

●

●

●

●

●

●●

●

●●●●

●●●●●●●● ●●

●

●●●●●●●●●

●

●●

●●●●●●●●

●

●

●●

●●●●● ● ●●●●

●●

●●●●●●●●●●●●

●●●●●

●●●●

●●●

●●

●●●

●

●●● ●

●●●●●●●●●

●

●●●

●●●●

●●●●

●

●●

●●

●●

●

●● ●

●

●●

●●

●

●

●●●

●

●

●●●

●

●

●●

●●●

●

●

●

●

●

●

●●●●●●●

●

●

●

●●●

●

●●

●●

●

● ●

●

●

●

●●

●

●●●●●●

●

●●

●

●

●

●

●

● ●●●●●

●

●●

●●

●

●●

●

● ●

●

●●●●● ●●●

●●●

●

●●

●

●

●●

● ●

●●●

●

●●

●

●●●●●

●

●●●●●●●

●●●●

●

●

●● ●●● ●●●●

●●

● ●●● ●●

●

●●●●

●

●●●●

●

●

●

●

●

●●

●●●●●●●●●

●●

●

●

●●●

●●●

●

●

●

●●

●●

●●●●●●●●●●●●●●●

●

●●

●

●

●●●●●●

●●●●● ●●

●●

●

●

●● ●

●●●●●●●

●●

●●●

●

●

●●●

●

●●●●●●●●●●●●●●●

●●●

●

● ●

●

●●●●

●●

●

●

●●

●●

●●●●●

●

●●

●

●

●

●●●●● ●● ●

●

● ●

● ●●

●●

●

●●

●●

●●●●

●●●●●●●●●●

●

●

●● ●

●●

●

● ●●

●●●●●●●● ●●

●

●● ●●●●

●

●●

●●●●

● ●

●

●

●●

●

●

●●●●●●

●●

●●●●●●●●●●●●

●

●

●

●●

●

●

●

●

●●●

●●

●

●●

● ●●●

●●

●●

●

●●

●●●

●●●●●●●

●

●●

●

●●●

●

●●

●

●

●

●

●

● ●

●

●●●●●●

●

●

●

●●●

●

●●●●●

●

●●●●●●

●

●●●●●●

●

●

●

●●●●●●●●●●●●●

●

●●

●●●●

●●

●

●●●

●

●

●

●●

●●

●●

●●

●

●

●●●●●

●●

●● ●●

●

●

●

●

●●

●

●●●● ● ●●●●●●

●●

●

●●

●●

●●●

●

●

●●●

●

●

●● ●●●●●●

●●

●●

●

●

● ●

●●●●●

●

●●●

●

●

●

●

●●●●●●●●

●

●

●

●

●

●●

●

●

●●●

●

●●

●

●

●●●●●

●

●●●●

● ●

● ●●●● ●●●

●●

●●●

●●●●

●

●

● ●●

●

●●●●●●

●

●

●

●●●●

●

●●

●

●●

●●●

●●

●

●●

●●●●

●

●●●

●●

●

●

●●

●

● ●●

●

●●

●●

●

● ●●● ●●

●

●

●●

●

●

●

●●

●

●

●

●●●●●

●

●

●

●●●●●●

●

●● ●●●●

●●

●●●●●●

●●

●●

●

●

●

●

●● ●●

●

● ●●●

●

●●

●

●

●●●●

●●

●●

●

●

●●●

●●

●●●

●●

●●●

●

●●

●

●

●

●●

●

●●

●

●● ●

●●

●● ●●●●●●●●●●●

●●

●●● ●●●●

●●

●

●

●

●

●●● ●●

●

●●

●

●● ●

●● ●

●

●

●●●

●●●●●●●●

●

● ●●●●●●●

●

● ●

●

●

●

● ●●●

●

●●

●●

●

●

●●

●

●●●

●

●

●●

●

●

●

●

●●

●●

●

●

●●●●●

●

●●

●

●●●●●●●●●●●●●●●●● ●

●●● ●

●●●●

●●

●●

● ●

●●

●●

●●● ●

●●

●

● ●

●

●

●

●●

●

● ●●

●●

●●

●

●

● ●●●●●●●

●

●

●●●

●●

●●

●

●●

●

●●●●●●●

●

● ●

●●

●●●●

●

●

●●

●

●

●●

●●●●

●

●

●●

●●

●●

●●●

●●●●●

●●●

●●

●

●

●

●

●

● ●

●●●

●

●

●●

● ●●●●●● ●●

●

●●●●●

●

●

●●●●●●

● ●

●●

●●

●●●

●

●●

●

●

●

●●●●●

●● ●

● ●

●

●●●●●●●

●

●●●●

●

●●●●● ●

●●●

●●●

●●●●

●●●●

●

●●

● ●

● ●●●

●

●●●

●

●●●●●●●●●●●

●

●

●

●●

●

● ●●●

●●

●

●

●

●

●

●

●

●●●

●

●●●

●

●●

●

●●●●●●

●●●●

●●●●●●● ●●●●●●●●●●●●●

●●

●

● ●●●●

●●●●●● ●●

●

● ●● ●

●●●

●

●

●

●

●●●● ●

●

●●●

● ●●

●●●●●

●●●

●

●

●●●●●●●

●

●

●

● ●●●●

●●

●

●●

●

●

●

●●●●● ●

●●●

●

●

●

●

●●●

●●●●●

●●

●●●

●

●

●

●●

●

●●

●

●●●●

●

●

●●

● ●●

●●●●

●

●

●●●

●●

●●

●

●

●

●●

●

●●● ●

●●●

●● ●

●● ●●

● ●●●●

●

●●●●●●● ●

●

●

●

●●●●

●

●

●●

●

●

●●

●●●●

● ●●●

●

● ●

●●

●

●●●●● ●

●

●●●

●

●●●●

●●

●●●

●●

●

●

● ●●●●

●● ●

●●

●

●●

● ●

●● ●●

●

●●● ●●●●●●●

●●

●●

●●●●

●

●●●

●●●●●●●●●●●●●

●●

●●●●●●

●

●

●

●

●●● ●

●

●

●

● ●●●●●●●●●

●

●

●●

●●●

●

●

●●●●

●

●●

●

●●

●●●●●●●●

●●

●●

●

●●

●●

●

●

●

●

●

●●

●●

● ●●●● ●

● ●

●●●

●●

●

●

●

●●

●

●

●●●

●● ●●●

●●●●●

●

●

●●●

●

●●

●●

●●●

●●●●●●

●

●●

●●

●●

●●●●

●

●●●

●●

●

●●

●

●●●

●

●

●

●

●●

●●

●● ●

●

●●

●

●●

●

●●●●

●●●●●●●

●

●●●●●●

●

●●●●

●

●

●●●

●●●● ●●●

●

● ●

●

●● ●

●

●●●

●

●●●●●

●●

●

● ●●

●

●●

●●● ●

●

●

●●●●●

●●● ●●●●

●●●

●

●●●

●

●

● ●●

●●

●●●

●

●●●●●

●

●

●●

●

●●●●●●●●

●●

●●●●●●●●●●●●●

●

●

●

●

●

●●●●●

●

●

●

●●

●●

●●●

●

●●

●

●●●

●

●●

●●

●●●

●●

●●●●●●

●

●●

●

● ● ●● ●●

●

●●●●

●

●●●●●●●

●●

●

●

●●●●

●●

●●●●●●●●●●

●●●

●

●

●●●

●

●● ●

●●●●●

●

●

● ●● ●●

●

●

●

●●●●

●●●

●

●

●

●●

●

●

●●●●● ●

●

●●

●●●●●●●

●●

●●●

●●

●●

●

●●

●●

●●●● ●

●●●●

●●

●●●

●●

●●●●

●●

●●●●

●●●

●

●●●●

●●

●●

●●●

●

●●●

●●

●●●●●

●

● ●●

●

●

●

●●

●●

●●

●

●●●●

●

●●●●●

●●●

●●●●●

●

●

●●

●

●●● ●●●●●

●

●●●●

●●

●

●●

●●●

●

●

●

●

●

●

●●

●●

●●

●●

●●●

●

●●

●

●

●

●● ●●

●

●●

●●●

●

●

●

●

●●●●●

●

●●●

●●●●●●

●

● ●●

●

●●

●●

●●

●●

●●

●

●●●●●

●

●●●●

●

●●●

●●●

●

●●●

●

●●

●

●●●●

●●●●● ●●●

●●

●●●

●●●

●●

●●

●

●

●●●●● ●

●

●

●

●

●●●

●

●●

●

●●●

●

●

●●

●

●●●

●● ●

●

●●● ●

●●●

●

●●●●●●●

●

●●

●

●

●

●●●●

●

●●

●●

●●

●

●●

●

●

●●●

●

●●

●

●●

●●●

●●●

●●

●

●

●●●

●●

●●●●●●●

●

●

●

●●●

●●

●●

●

●

●

●●●●●●●●●●

●

●●● ●●

●●

● ●●● ●

●

●

●

●●●

●

●

●●●●●●●

●

●●●● ●●

●

●●●

●

●

●

●

●●●

●●●

●

●

●

●●

●

●

●●

●●●●●

● ●●●●●● ●●●●● ●

●●

●●

●

●●●

●

●●

●●●

●

●

●●

●●

●

●●●● ●

●

●

●●

●● ●

●●

●

●● ●

●●

●

●

●

●

●

●

●

●●

●

●

●

●●

●

●●

● ●●●●●

●

●●●●●

●●●

●●●●●●● ●●

●

●●●●●●

●●

●●

●

●●●

●●●

●

●●

●

●

●

●●

●●●●●

●

●●●●

●● ●●●●●●

● ●●

●

●●

●●●●●

●

●●

●

●● ●

●●

●●●

●

●●●●●●

●● ●

●●●

●

●●

●

●●

●

●●●●●● ●●

●●

●

●

●

●

●● ●●●

●

●●●●●●●●●

●●

●

●●

●

●●●

●●●●

●● ●●

●●

●

●●

●

●●●

●

●

●

●●●●

●

●●●●●●

●

● ●●

●

●●

●

●●●●●

●●●●● ●●●

●●●●●●●

●●●●

●

●

●

●

●●●●●●●●●●●●●

●●●

●●●●

●

● ●●

●

●

●

●

●●

●

●

●●●●

●

●

●

●● ●

●●

●●

●

●●

●●●●

●

●

●

●●●●

●●

● ●●●

●

●

●●

●●●

● ●●●

●●

●●●

●

●●

●

●

●

●

●●●

●●

●

●

●

●●

●●●●●

●●

●

●

●●●

●●

●●●

●

●

●●

●●

●

●

●●●

●●●●●●

●

●●●

●●●●●

●

●●

●

●●●

●●●●●

●

●

●●●

●●

●●●

●

●

●

●

●

●●

●

●

●

●

●

●●

●

●

●

●● ●

●●●●

●●●

●

●●●●

●

●●●

●●●

●

● ●●●●

● ●

● ●

●●●

●●●

●●●●

●●●

●●

●● ●

●

●●●●

●

●●●●●●●●●●

●

●

●

●

●

●●●●

●

●

● ●●●

●●●●

● ●●●

●

● ●●

●●

●●●●●

●

●●

●

●●

●

●● ●●●● ●

●●

●●

●

●

●

●●

●

●

●●

●

●

●

●

●●●

●●

●

●

●

●

●

●●

●●

●

●

●●●

●

●

●● ●

●

●●●●●●●●●

●

●●

●

●●●

●●●

●●

●●

●●●

●

●●●●

●●●

●●

●●●

●●●●●●●●●●

●

●

●

●●

●

● ●●●●

●●●

●●

●●

●

●●

●●

●●

● ●

●●●

●

●●

●●

●●

●●

●

●

●●●●

●●

● ●● ●●●

●●●●●●●

●

●

●●●●●●

●

●●●●●●

●

●●●●●●●

●

●●● ●●

●●●● ●●●

●●●

●●

●

●

●●

●●●

●

●

●

●●●

●●●●

● ●●

●

●●●●

● ●●

●

●

●●●●

●●

●●●

●

●●●

●● ●●

● ●

●

●●● ●●●

●●●

●● ●●

●

●●●

●

●

●

●

●

●●●

●

●

●

●

●●

●

●●

●

●

●●

●●

●

●●

●

● ●●●●●●●

●

●●●●●●

●

●●

●●

●

●●●

●●●

●●

●●●●

●●●

●

●

●●● ●●● ● ●

●●●●●

●

●●

●●●●

●

●

●●●●

●●●●●

●●

●●

●

● ●●

●

●

●●

● ●●●

●

●●

●●

●

●

●●●●●●

●

●

●

●●●●

●●

●

●●

●●●

●●●

●

●●

●

●

●●●

●●●●●●●●●

●●

●

●

●●

●●

●

● ●●

●

●● ●●●

●

●●●

●

●

●

●●●●●●●

●●●●

●

● ●●●●●

●●

●●● ●

●

●●●●● ●●●●●

●

●●●

●

●

●

●●●●

●

●●

●

●●●

●

●●

●●

●

●

●

●

●●●●●● ●

●

●●

●●●●

●●

●●●●●

●●

●

●

●●●

●●●

●

●●

●●●●●

●

●●●

●●

●●

●●●●●●

●●●

●●●●

●

●

●

●

●

●●●●●●

●

●● ●

●●

●

●●●

●●●

● ●●

●

●

●●●●

●

●

●●●●●●●●●●●

●

●●●

●●●●●●●●

●●●●

●●

●

●●●

●●●●●

●●●●●

●

● ● ●

●

●

●

●●

●

●

●●●

●

●● ●●

●●

●●●

●

●

●●●●●●●

●●

●●

●●●

●

●

●

●

●

●●● ●

●● ● ●●●●

●

●

●●

●●

●●

●

●●●

●

●●

●

●

●

●●●

●

●●●

● ●●●

●

●

●●●

●●●●

●

●

●●●●

●●●●●●

●●● ●● ●

●

●

●●●

●

●●●●●●●●●

●●

●●●

●●●●●

●

●●

●●●●

●●●●●●

●●

●●

●●●●●●●

●

●

●

●●●●

●●

●

●●

●●●

●●●●

●●

●

●

●● ●●

●●

●

●

●

●

●

●

●●

●●

●●

●

●

●

●●●

●

●●●

●●●●●●●

●

●

●

●

●

●

●●

●

●

●

●●

●●●

●

●●

●

●

●

●●●●● ●●●●

●●●●●●●●●●●

●

●

● ●●

●●

●●●

●

●●

● ●●●● ●●●

●

●●● ●

●

●

●

●

●●●●

●

●●

●

●

●

●

●

●●

●●●

●

●

●●

●

●●

●

●

●●●

●●●

●

●

●●

● ●

●●●

●

● ●●

●●● ●

●●

●●●

●●●●●

●

●●●

●

●

●

●

●

●●

●●●

●

●

●● ●●●

●●●●

●●

●

●●

● ●

● ●

● ●●

●●●●

●● ●

●●●●● ●

●●

●●●

●

●

●●●●●

●

●●●

●●

●

●

●●

●

●

●

●

●

●●●

● ●

●

●

●●●

●●

● ●●●●

●●●

●

●● ●

●●

●

●●

●●●●●●●●●●●●

●

●●●●

●●

●

●●

●●

●

●

●●

●

●

●● ●●●●●

●

●●●●●

●●

●

●●

●●

●

●●

●●

●● ●●●

●

●●

●

●●●●●●●●●●●●●

●

●●●●

●

●●

●●

●●●●●●●●●

●●

●

●

●●

●

●● ●●

●

● ●●

●●●●●

●●

●

●●●●●

●

●

●

●

●

●

●●

●●●

●

●●●●●

●●●●●●

●●●●

● ● ●●

●

●●●●●●●●●●●

●●●●●

●

●

●

●●

●●●●

●●

●

●●

●●

●● ●●●● ●●

●

●●

●

●●

●●

●

●

●●

●

●●●●

●●●●

●

●

●

●

●●

●

●●●●●●

●

●●

●

●

●●

●

●

●

●

●

●

●

●●

●●●●

●

●●

●●●●●●

●

●

●

●●

●

●

●

●●●●●●●●● ●●

●●

●

●●●

●

●

●●

●●●●●●

●●

●●●

●●

●

●●●

●

●

●

● ●●

●●●●

●

●●●●●

●

●

●

●●

●

●●●●●

●

●

●●

●

●

●●●● ●

●●

●

●

●●●

●

●●

●

●

●●●●●●●

●●●●●

●

●●●●●

●●●● ●●

●

●

●

●●●

●

●

●

●

●

●●

●●●●●

●

●

●

●

●

●

●●●

●

●●●●

●●

●●

●

●●●

●● ●●●

●●●● ●

●●

●●

●●●●

●

●

●

●●●

●

●●

●●

●● ●

●

●

●● ●●

● ●

●● ●

●

●

●●●●●

●●

●●

●

●

●●

●●●●

●

●

●●●●

●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●

●●●

●

●

●

●

●●●

●

●

●

●

●

●●● ●●●●●●●

●

●●●●

●●●●●●●●●●●● ●●●

●

●

●●●●●

●

●●

●●●● ●●●●●●

●●●

●

●●●●●●●

●●●●●●

●●●●●

●

●

●●

●

●●

●●

●●

●

●

●●●●

●

●●●●●●●●

●

●

●

●●

●●●

●

●

●●

●

●●●●●●●●●

●

●●●●

●●●●●●●●●●●●●●

●

●●●

●

●

●●●

●

●

●

●●●

●

●●●●●●●●●●●●

●●

●

●

●●●●●●●● ●

●

●●●

●●●●●●●●●●●●●

●●●●

●

●●●●●●

●

●●

●

●

●●

●●

●●●●●

●

●

●●

●●●●●●●●●●

●●

●●●●●●

●

●

●●

●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●

●

●●

●

●●●

●

●

●

●●●

●

●●●

●

●●

●

●● ●

●●●●●●

●●●●

●●●●

●●

●●●

●●●●

●

●

●

●

●

●

●

●

●

●

●●

●

●

●● ●

●

●●●●●●●● ●●●●●●●

●●

●

●●

●●●●●●●●●●●●●

●

●●

●●●●●●●●●●●●

●

●

●●● ●● ●● ●●●●●●●●●

●

●●● ●

●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●

●

●●●●

●●

●

●●●

●●●●●●●

●

●

●●●●

●●

●

●

●●●●●●●●●●●●●●●●●●●●

●●●

●

●●

●●●●●●●●

●

●●

●

●●●●●

●● ●●●

●●●●

●

●●●

●

●

●●●● ●●●

●●●●●●●●● ●●●●

●

●●

●●●

●

●

●

●

●

●●● ●●●●

●

●

●● ●

●

●

●

●

●●●●●●●●●●

●

●●●

●●●

●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●

●●●

●

●● ●●●●●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●●●●●●●

●●●

●●●●●●●

●●●

●

●●

●●●

●●

●

●

●●●

●●

●●●

●●

●

●●●●●

●●●●●

●

●

●●●

●● ●

●●●●●●

●●●●●●●

●

●

●

●●●●●●●●

●●

●●●●●●●●●

●●

● ● ●●

●●●

●●● ●●●●●●●●●●●●●●●●●●●

●

●● ●●●●●●●

●

●●●●●

●●●

●

●●

●

●

●●●●●●●

●

●●●●●●●●

●

●

●●●●●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●● ●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●

●●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●

●

●●●●

●●

●●●●●●●●●

●

●●●●●●●●●●●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●

●

●●

●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●

●

●

●

●

●

●

●

●●

●●●●●●●●●●●

●●●●● ● ●●●●●●●●●

●●●●

●●●●●●● ●●●

●

●

●●

●●●●●

●

●●●●

●●

●●●●

●

●●●

●

●●●●●●●●●●●●

●

●●●●●●●●●●

●

●

●● ●●●

●●●●

●

●●

●

●●●

●

●

●●●●

●●

●

●

●

●

●●●●●●●●

●●●●●●●●

●

●●

●

●●●●●●

●

●●●●●●●

●

●●●

●●

●

●

●● ●●●

●●●●

●●●●●●●●●●●●●●

●● ●●

●●●●

●●

●●●●●●●●●●●●●●

●●

●●●●

●

●●●

●●●

●●

●●●●●●●●

●●●●●●●●●●●●●●●●●●●●

●

●

●●● ●●●●●●●●●●●●●●●●●●●

●●

●●

●●

●

●●●●

● ●●

●●●

●●●

●

●●●●

●●●●●●●●●●●●●●

●●●●

●

●●●

●

●

●●●

●●

●●

●

●

●●●●

●

●●●

●

●●●●●●● ●●●●●●●●●●●●

●

●●●●●●●

●

●

●

●

●●●●

●

●●●●

●

●●●●●●

●

●●●●●●●●●●●●●

●●

●●●●●●

●

●

●

●

●

●

●●

●

●●●●●●●●

●●

●●

● ●

●●

●●●●●●●●●●●●

●

●●●●●●

●●

●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●●●

●● ●●

●

●●●

●

●

●

●●●●●●●

●

●

●

●

●

●●●

●

●●●●

●

●●●●●●●●●

●

●

●

●

●●●

●

●●●●●●

●

●●

●●

●●●●●●●

●

● ●●●

●

●●

●

●●

●●●●

●

●●●●

●

●●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●● ●

●●

●

●

●

●●●●●●●●●●●●●

●

●●

●●●●●●●

●

●●

●●●●●

●●

●

●

●

●

●● ●●

●

●●●●

●

●

●

● ●●

●●

●

● ●

●●●

●

●●●● ●●●●●●●

●

●

●●

●●●●

●●

●●●

●

●●●●●●●●

●

● ●

●●●●●●●●●●●●●●●

●● ● ● ●●●●

●●

●●

●

●●●●●

●

●●●

●●

●

●●●

●●●

●●● ●●●●●

●

●

●●●●

●

●●●

●●

●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●

●

●

●

●

●

●●

● ●●

●●

●●

●

● ●

●

●

●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●●

●●

●●●●●

●

●

● ●●●●●

●

●

●

●●●●●●●●●

●

●● ●●

●●●●●●●●●●●●●

●

●●

●●●●●●●●●●●

●

●● ●●

●●● ●●●●●●

●● ●

●● ●● ●

●

●

●

●

●●

●

●

●

●

● ●●

●●

●●

●●

● ●●

●

●

●

●●●

●

●

●●●●●●●●●●

●

●

●

●●● ●●●●●

●●

●

●

●● ● ●●

●●●

●

●●

●

●

●●●●●

●

●●●●

●

●

●●●●●●●●●●●●●●●●●●● ●●●

●

●●

● ●●

●

●

●●●●●●●●●●●●●●●●●●●●

●●●● ●

● ●

●●

●●●●

●

●●●●●●●●●●●●

●

●

●

● ●●

●

● ●

●

●●

●●●●●●●

● ●

●

●

●

●

●

●

●

●

●●

●

●●●●●●●

●●

●

●

●

●●●●●●●

●

●●

●●●

●

●●●●

●●●

●

●●

●

●●●●●●●●●●●●●●●● ●

●

●●

●● ● ●●●

●●●

●

●

●

●

●●

●

●●●●

●●

●●●● ●● ●

●●

●

●

●●

●●●

●

●●●●●●●●●●●●●●

●

●●

●●

●●● ●●●●

●●●

●

●

●●●

●

●●

●

●●●●

●

●●●

●

●

●

●●

●●●

●●

●

●

●●

●

●●

●●

●

●●

●●●

●

●●●

●●●●

●●●

●●●●●●

●●

●●●●●● ●

●

●

●●

●●●● ●●●

●

●●

●

●●●●●● ●●●●●

●●●●●●

●●

●

●●

●

●●

●●

●●

●

●

●

●●

●●

●

●●●●

●

●●●

●

●

●●●●

●

●●

● ●●●

●

●

●●

●

●

●●

●●

●●

●●

●

●●

●●

●

● ●

●

●●

●

●

●●●●●

●

●●●●●●●●●

●●●

●●●●

●

●●●●

●

●●

●

●

●●●

●

●

●

●

●

●●●

●

●

●●●

●

●

●

●

●●

●

●

●

●

●

●●●●●●

●●●●●

●

●

●

●

●●●●

●

●

●

●

●

●

●

●●

●

●

●

●

●

●

●

●●

●

●

●

●●

●

●●●●●

●

●

●●

●●

● ●●

●●

●●●●●

●●

●●●●●

●

●●●●

●

●

●●●

●

●

●

●

●● ●

●● ●●

●●●● ●●

●

●

●

●●

●●●●

●

●●

●●

●●

● ●●●●●

●

●

●

●

●●

●

●●●

●●●●●

●●●

● ●●●

●●

●●●●

●

●

●

●

●●●●●●●● ●

●

●

●●

●●●●

● ●

●

●●●● ●●

●●

●

●

●

●

●●

●

●●

●●● ●

●

● ●

●

●

●●●●●

●●●●●

●

●●

●● ●●

●

●

●

●

●

●●●

●●

●

●●●●

●

●

●●● ●

●

●●

●

●

●

●●●

●●

●●●●●●●●●●● ●●●●●●●●●

● ●

●

●

●

●

●●●●● ●●●

●●

●●●

● ●●

●●

●●●

●● ●

●

● ●●

●●●●

●

●●●

● ●● ●●

●●

●

●

●●

●●

●●●

●

●●●●●

●

●

●

●

●●●

● ●

●

●●

●●●●

●●

●

●●●

●

●

●●

● ●●●●●●●

●●●

●●

●

●

●●●

●●

●

●●●●

●

●

●

●

●●

●

●●● ●●●●

●●

●●●●

●●●●●

●

●

●●

●

●

●

●

●

●

●

●●

●●

● ●●

●

●

●●●●●

●

●

●

●

●

●

●

●●●●

●●●

●

● ● ●●

●

●

●

●

●●

●●● ●

●

●● ●

●

●●●

●●●

●●●●● ●●

●

●●●●●●

●

●

● ●●●

●

●

●

●●●●

● ●

●

●

●

●

●● ●● ●●●●●

●●

●

● ●

●●

●●

●

●

●

●

●●●●●●

●

●●●●●●●●●●

●

●● ●●

●

●

●

●

●●

●●●

●

●

●●●

●●●●●

●

●●●●

●

●

●● ●

●●●

●●●●●

●●●

●

●●●

●

●●●●

●

●

●

●●●

●

●●

●

●●

●●●●●●●●

●●

●●●●●●●●

●

●●

●●●●

●●

●

●

●

●●

●

●●

●●●●●●●●●●●●●●●

●

●●●●●●●

●●

●●●●●●●

●

●

●●●

● ●

●●

●

●

●

●●●●●

●●

●●

●●●●●

●

●●●

●

●●

●●

●

●●●●●●●

●

● ●●

●●●●●●

●

●

●●●

●●●

●●●

●●●

●

●● ●●

●

●

●

●●●

●

●

●●

●

● ●●●●●●

●●

●●

●

●●●●●●●●●● ●●●

●●●●●● ●

●

●

●

●

●●●●

●

●●●

●

●

●

●

●● ●●

●

●●

●●

●●

●●

●

● ●

●

●

●●●

●

●

●● ●

●●●●

●●●●●

●

●

●

●●

●●

●

●

●

●●●●●●●

●

●●

●

●

●

●

●

●

●● ●● ●

●●

●

●

●●●●

●●

●

●

●

●●●●

●●●

●

●

●●

●

●

●●●

●

●●

●

●

●●

●●●

●

●

●

●

●●●●●●

●

●

●●

●●●

●

●●

●●●●●●

●

●

●●

●

●

●●●

●●●●●●

●

●●●●● ●

●●●●●

●

●

●

●

●●

●●●●

●●

●

●

●

●

● ● ●●●●●●

●

●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●●● ●●

●●

●

●●

●

●

●●●● ●

●

●●

●●

●

●●●● ●●

●

●

●●

●●

●

●

●●

●

●

●

●

●

●

●

● ●●●

●●●

●

●

●

●●

●●●

●

●

●

●●●●

●●●

●

●

●

●

●

●●

●

●●●

●

●

●● ●

●

●

●●

●●●●

●

●

●

●

●●● ●

●●●

●●

●●●

●●

●●

●

● ●●

●

●●

●●

●

●

●

●

●

●

●●●

●●

●●

●●

●

●

●

●

●

●

●●

●●●

●●

●●

●

●

●●●●●●●●●●

●

●●

●

●●

●

●

●

●

●●

●

●

●

●●●●●

●

●●●

●

●●●

●

●

●

●● ●●●●

●

●

●●●

●

●

●●●●

●

●● ●

●

●

●●

●●

●●●

●●

●● ●

●

● ●

●

●

●

● ●● ●●●

●●●●●●

●

●

●●

●●●●

●

●●

●

●

●●●

●

●

●●

●

●

●

●●

●

●●●●●

●

●●

●

●

●

●●● ●

●●●●●

●

●

●

●●●●●●●●

●●●

●●

●

●

●●

●●●●

●●●●●●●

●●

●

●●● ●●●

●

●

●●

●●

●●● ●

●●●

●●●●

●●

●●●●●

●●

●

●●

●●

●●

●●●●

●●

●●●●

●

●●●●●

●●

●●●●●●

●

●

●

●●

●●●●●

●

●●●●

●

●

●●● ●●

●●

●

●

●

●

●

●

●●

●●

●●

●●●

●

●●

●

●

●

●●

● ●●●●

●

●

●●

●

●

●●●●●●●●●

●●

●●●

● ●

●

●●●●●●● ●

●●●●●●●●

●

●●●●●

●● ●

●

●

●

●●

●●●

●

●

●● ●

●●●

●

●

●●

●

●

●

●

●●● ●●

●

●

●●●

●●●●

●●●

●

●●●●

●

●

●●●● ●●●●●●

●●● ●●

●●●●●●●●●●●●●●●●●●

●●●●●●● ●●

●

●●●●●

●

●●●

●●●●

●

●

●●

●

●●●●●●●●●

●●●

●

●

●

●●

●

●

●● ●●

●

●●●●●●●

●●

●●●●●●●●●● ●●●●

●

●●●

●

●●●●●●●●●●

● ●

● ●●●●●●●

●

● ●● ●●

●●●●

●●●●

●

●●

●●●●●●●●● ●●●●●●●

●

●●●●●●●●●●●●●●

● ●●●

●

●●●●●●●●●●●●●●●●●●

●●

●●●●

●

●

●

●●●

●●●●

●●●

●

●

●●

●

●● ● ●●●●

●●●●●●●●●●●●●●

●

●●●●●

●

●

●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●

●●●●●●●●

●●

●

●●●●●●●

●

●

● ●●●●

●

●●●●●●●●●●●●●●●●

●●

●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●

●

●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●●

●

●●

●

● ●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●

●

●●●●●●●●●●

●

●●●●●●●●●

●

●

●●

●●

●

●●●

●

●●

●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●

●●●

●

●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●

●●

● ●●●●●●●

●

●●●●●●●●

●●

●

●●●●●

●

●

●●

●

●●●●●●●●●●●●●●●●●●●● ●●●●

●

●●●●●●

●

●

●●●●

●

●●●●●●●●●

●

●●●●●●

●●●●

●●●●●●●●●●

●

●● ●●

●

● ●●●

●

●●●●

●

●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●

●●●● ●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●

●

● ●●●●●●●●●●●●●

●

●● ●●●●●●●●●●●●●●●

●●●●●●●●●●●●●

●

●●●●●●●

●

●●●●●●●●●

●

●●●●●●●

●

●

●

●

●●●●●●●●

●

●●

●●●●●●

●●●●●●●

●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●

●

● ●●

●

●

●

● ●●●●●●

●●

●●●●●●

●

●●●●●●●

●

●●●●●

●

●●●●●● ●

●●●●●●●●●●●●●●

●●

●●

●

●

●●●

●●●

●

●● ●●●●●●●●●●●●●●●●●●●●●

●

●●

●●●●

●

●●

●●●●

●

●●●

●

●●●●●●

●

●

●●●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●

●●

●

●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●

●●●●●●●●●●

●

●●●●●●●●●

●

●●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●

●

●●●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●

●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●

●

●●●

●

●●

●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●●●●●●

●

●

●●

●●●

●●●●●●●●

●

●●●

● ●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●● ●●●

●

●●●●●●●●●●

●

●●●●●●●●●●

●

●●

●

●●●●●●●●● ●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●● ●●●●●●●●●

●●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●●●●●●● ●●● ●●●●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●●

●

●

●

●

●●

●

●●●●●●●●

●●●●

●●

●

●●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●

●●●●●

●●●●●

●●

●

●●●●● ●●

●

●●●●●●●●●●

●●

●●●●●●●●●●●●

●

●●●●

●●●

●●●

●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●●

●

●

●●●●●●●

●●

●●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

● ● ●

●●●

●

●

●●

●●●●●●●●●●●

●

●●●●●●●●●

●●●

●●●●●●● ●●

●●●●●●●

●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●●●

●

●●

●●●●

●

●

●●●●●●●●●●●

●●●●

●

●●

●

●

●

●●

●●●●●

●

●

●

●●●●●

●

●

●●●●●

●●

●

●

●

●

●

●●●●●● ●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●

●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●● ●●●●

●

●●●●

●

●●●●●●●●●●●●●●●●●●

●

●●

●

●

●●

●

●●●●●●●

●

●●●●●

●

●

●●

●●●

●

●●●● ●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●

●●●●●●●●●●●

●

●● ●

●●●

●●●●●●●

●

●●● ●●●●

●

●● ●

●●

●●● ●

● ●●●●●●

●

●●

●●●●●●●●

●

●

●

●●

● ●

●

●● ●●●●●●●●●●●●●

●●●●●●● ●●

●

●●●●●

●

●

●●●●

●

●

●

●

●●●●●● ●

●

●●

●●●

●

●●●●●●●●

●

●●

●●●●●●

●

●●●

●

●●●●●●●●●●● ●●●●● ●●●●

●

●

●

●

●●

●

●●●●●● ●●●●●●

●

●●●

●●

●

●●●●●

●

●

●

●

●

●

●●

●

●●●● ●●●●●●●●●

●

●●●●

●●

●●●

●●●

●●●●●

●

●●●●●

●●

●●●

●

●●●

●

●

●●●●●

●●

●●●●●●●●●

●●

●

●

●●

●● ●●●●

●

●

●●●●●

●

●●

●

●●●

● ●●●●

●

●●

●

●●

●●

●●●●●●●●

●

●

●

●●

● ●

●

●●●●●

●●●●●●

●

●●●

●

●

●

● ●

●

●

●

●●●●●●●

● ●

●

●

●●●

●●●●

●

●●

●

●●

●

●

● ●●●●●●●●●

●

●●●

●

●●●●●●●●

●

●

●

●●●●

●●●●●●●●●●●●●●●●●

●

●

●

●●●

●

●●●●●

●●●●●●●●●●●●●●●●●●

●

●

●

●

●●●

●

●●●●

●

●●●

●

●

●

●●

● ●

●

●●

●●

●●

●

●●

●

●

●

●

●● ● ●●●●●●●

●●●●

● ●●●●●●●●●

●

●●

●●

●●

●

●

●●●

●

●●

●●●

● ●●

●

●

●●●●●●●

●●

●●●●●●●

●

●

●

●

●●●●

●

● ●●●●

●

●

●●●●●●●●●●●●●●

●●

●●●

●

●●

●●●●

●● ●●●

●

●●

●

●

●

●

●

●

●

●●●

●

●●

●

●● ●●●●

●

●●

●

●●

●●●

●●

●

●●●

●

●●

●

●

●●● ●●●●●

●

●●●

●●

●●

●●

●●●

●

●●

●

●●

●

●

●●

●

●

●

●●●●●●

●

●●●

●

●●

●

●

●

●●●

● ●

●●●●●●●●●●●●●●●●●●●●●●●

●●● ●●●●●

●

●

●●●●●

●

●

●

●●●

●

●● ●●●●●●

●

●

●●

●●●●●●●●●

●●

●

●

●●●

●●● ●●●

●

●

●

●●

●

●●

●

●

●●●●●●●● ●●● ●●

●

●

●

●

●

●●

●

●

●●

●●●●

●●

●●●

●●

●●

●●●●

●

●●

●

●●●●●●

●

●

●●

●

● ●

●

●

●

● ●●●

●●

●

●

●

●●● ●

●●

●

●●●

●●● ●

●●

●

●● ●

●

●

●

●●●●●●●

●

●●●●●●●●●● ●● ●●●

●

●●●●●●●●●

●● ●

●

●

●●●●●

●●●

●

●

●●●●● ●

●

●●●●

●

●

●●

●

●

●

●

●●●●

●

●

●

●●

●

●

●●

●●●●●●●●●●

●●●●●●●●●

●●●

●●●

●

●

● ●

●●

●●●●

●

●●

●

●●●●●●●●● ●●

●

●●

●

●

●●

●

●

●

●

●●●

●●●●

●

●

●●●●

●●●●●●●

●●

●●●

●

●●●

●

●

●

●●

●●●

●●

●●●

●●

● ●●

●●

●

●

●●●●●●●

●●●● ●

●●●●

●

●

●

●

●●● ●●

●

●

●

●

●

●●●

●●●

●

●●●●●

●

●

●

●●● ●

●●●

●

●●

●

●

●●

● ●●

●

●

●

●

●●

●

●

●●●

●

●

●●

●●

●●●●●●●●●●●● ●● ●

●

●

●●●

●

●●

●

●

●●

●

●●●

●●

●●

●●●●●●●●●

●

●

●●

●●

●●●

●

●●●●●

●

●● ●●

●●

●●

●

●

●●

●

●● ●

●

●

●

●●

●●●●

●●

●●●●●●●

●●

●● ●

●●

●●●●●

●

● ● ●

●

●●● ●●●●

●●●●●

●

●●●●●●

●

●●●●●●●

●

●●

●●●●

●

●●

●●

●●

●

●

●●

●●

●●● ●

●●

●

●●

●

●●●●● ●

●

●●

●

●●●●●

●●

●●●●●●●

●●●●●●●●●●●

●

●●●

●●●

●

●

●

●

●

●

●

●● ●●

●●●

●

●

●●●

●

●●●●●●●●

●●

● ● ●●●

●

●●●●●

●

●

●

●●●

●

●●

●

●●●●●●●●

●●

●

●●●●●●

●

●●●

●

●●●●●● ●●●●●●●●●

●●

●●

●●

●

●●●●●

● ●●

●●●

●●●●●●●

●

●●●

●●●●● ●

●

●

●●

●●●●

●

●●

●

●

●

●●

●●

●●

●●

●

●

●●●●

●

●

●

●●

●●● ● ●●

●

●●

●

●●

●● ●

●●

●

●●

●●

● ●

●

●●

●

●●●● ●●●

●

●●●●●●●●●●●

●●

●

●

● ●

●●●

●●

●

●●

●

●

●●●●●

●

●

●

●

●●●

●●

●●●●

●

●●

●

●●●●●●●●

●●

●

●

●

●

●

●●●

●●

●

●

●

●●●●●

● ●●●

●

●

●

●

●●

●●●● ●

●

●

●

●

●● ●●● ●

●●

●

●●●

●

●

●

●

●●

●

●

●

●

●●●●●●●

●

●

●

●●●●●●●●●●

●●

●

●

●

●●●●

●

●●

● ●

●●●●

●●

●

●

●

● ●●●●●●●

●

●●

●●

● ●●

●●●●●●●●

●●

●

●●●●

●

●●●●●●●●●●●●●●●●●

●●●

●●●●●●●●●●●●●

●●●

●●●

●

●

●

●

●

●● ●●●●

●

●

●●●●●●●●●●●●

●●

●

●●●●

●

●●

●●

●●

●●●

●●

●● ●

●

●

●

●

●●●● ●●

●

●●

●●●●

●●●

●

●●●●●●

●●●●● ●●●●●

●

●●

●

●●●●

●● ● ●

●●

●●●●●●●

●

●●

●

●●

●●● ●●

●●●●

●

●

●

●

●

●●●●●●● ●●

●●

●

●●

●●

●●

●

●●●●●●

●

●

● ●●●●

●

●●●

●●

●●●

●●

●

●

●

●

●

●

●● ●●

●●●

●

●●●

●●●

●●●●

●●●● ●●●●●●●●●●●●●●●●●

●●

●●●

●

●●

●

●

●

●

●

●●●●

●

●

●●

●

●

●

●

●●●●●●●●

●●

●

●●●●

●

●●●●●●●●●●

●

●●●●●

●

●●

●

●

●●●●●●●●●●

●

●

●●

●

●● ●●

●●●

●●

●●●●●

●●●● ●

●●

●

●

●●

●●

●

●

●●

●

●●●●

●

●

●

●

●

●●●●●

●

●●

●

●

●●●●●●●●●●

●●

●●●●●

●

●

●● ●●

●●●●●●

●

●

●●●●

●

●

●●●●●●●●●●●●●●

●

●●

●●

●

●●●●

●

●

●●●●●●

●●●●●●●●

●

●

●

●

●

●●●

●●

●

●●

●

●

●●●●

●

●

●●

●

●

●●

●●

●●●

●●●

●●

●

●●●

●●●●●●●

●

●●

●●

●

●

● ●

●

●

●●

●

●●

●

●

●

●

●●●●

●

●

●

●

●●●

●●

●●●●

●●●●● ●

●

●●

●●●●●

●●●

●● ●

●

●●●

●●●●●

●

●

●●

●

● ●●●●● ●●

●

●●●

●

●●●●

●

●

●

●

●●

●

●

●

●●

●

●●●

●

●

●●

●●

●

●●

●●● ● ●

●

●

●

●

● ●

●●●●●

●

●●

●

●●●

●●

●

●●

●●

●

●

●

● ●

●

●●

●●

●●

●

●●

●●

●

●●

●

●●●

●

●●

●●

●●

●

●

●

●

●●

●

●

●

●

●

●●●●

●●

●

●● ●●●●

●●

●

●

●●

●

●

●●

●

●●

●

● ●●

●●●

●●

●

●

●

●●

●●●●

●

●

●●

●

●●●●●●

●

●

●

●●●

●●●●●●

●●

●

●

●

●●

●●

●

●

●●●●

●●●●●

●●

●●●●

●

●●

●

●●●

●●

●●

●●●

●●●●

●

●

●

●

●●●●●

●●●●●●

●

●● ●●●

●●●

●● ●

●●

●

●

●

●

●●

●●●●●

●

●●●●

●

●

●

●●

●

●●●●●●

●●

●●●●●●●

●

●

●

●●

●

●●●●●●

●

●●●●●●●●●● ●●●

●●

●

●

●

●●●●●

●

●

●

●● ●

●●

●●● ●●

●

●

●●

●

●

●●

●●

●

●

●

●

●

●●●

●

●

●●

●

●

●

●●

●

●●●●●●●●●●●●●●

●

●

●

●●●

●

●●

●

●

●

●●

●●●●●●●●

●●●●●●

●

●●

●

●●

●●

●

●

●●●●●

●●

●●

● ●

●

●

●

●

●●●

●● ●●●

●●

●●●●●● ●●●●●

●●

●

●

●●

●●●

●●●●●●●●●

●●●●

●

●●●●

●●

●

●

●

● ●

●

●●

●

●●●●●●

●

●●●

●●●

●

●●●●●

●●●●

●●

● ●●●

●

●

●●

●

●

●●

●●

●● ●●●●

●

●●●●

●

●●●

●●●●●●●●●●

●●●●

●

●

●

●●●

●●●●

●

●● ●●

●

●● ●●●●●

●●●

●●

●●●

●●

●●

●

●●

● ●●

●●●

●●

●●

●●

●●●

●

●● ●● ●

●

●

●●

●

●

●●●●●●●●●

●

●●●● ●●●

●●

●

●

●●

● ●

●

●●

●●●●●●●●●●●●●

●●●●●●●

●●●

●

●●

●●●

●●●●

●●

●●●●●● ●

●

●●

●●

●

●●

●

●

●

●●●●

●

●●

●●●

●●

●●●

●

●●●

●●●

● ●

●●●●

●●

●

●

● ●

●●●●● ●● ●●

●

●●● ●

●

●

●

●●

●

●

●

●● ●●●● ●●●

●●

●●

●

●

●●

●

●●

●

●

●

●

●●●●●

●●●

●

●

●

●●

●

●

●

●

●

●

●

●●

●

●

●

●

●

●

●●●

●

●

●

●●

●●●

●●●●

●●

●●

●

●●

●

●

●

●●●

●

●●●●

●

●●●●●●

●

●

●●

●●

●

●●

●

●

●●

●●

●

●

●●●●●●●●●● ●●

●

●

●●●

●●●●●

●● ●●

●

●

●

●●

●

●●●●

●●●

●

● ●

●

●

●

●

●●●

●●●

●

●

●

●●●●●

●

●

●●●●●

●

●

●●●●●●●●●●●●

●●

●●●●

●●

●

●●●

● ●

●●

●●

●

●●●

●

●

●●

●●●●●

●●● ●

●

●●

●

●●

●

●

●●●●

●●●●●●●●●●● ●●●

●

●●

●

●

●●●

●●

●●●

●●●

●

●●●●●

●

●

●

●

●

●

●

●

●●

●●●

●●●

● ●

●●●

●

●

●●

●

●●●●

●

●

●●●●●

●

●

●●

●●

●●

●●

●●

●●

●

●●●

●

●●

●●

●

●

●

●

●●

●●●●●●●●●●●●

●

●

●●●●●●

●

●

●

●

●●

●●●

●●●●●●●●

●

● ●●●●●

●●●

●●

●

●

●●●

●●

●●

●● ●●

●●●●

●

●

●●●●●

●●

●

● ●●

●●

●● ● ●●●●

●●●●

●

●●●●●●

●

● ●●●

●●●

● ●

●●●

●

● ●●

●

●

●

●●

●●●

●

●●●●●●● ● ●●●● ●●●●

●

● ●●●

●

●

●●● ●●●●●●●●

●

●

●●

●●

●●●

●●●

●●●

●

●● ●●●

●●

●●●●●●●●●●

● ●

●

●

●●

● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●● ●●● ●

●

●●●

●●●

●

●

●

●●

●

●

●● ●

●

●●●●●●●●●

●

●

●● ●●●● ●

●●

●●●

●●

●●●● ●

●

●

●

●●●

●

●

●●●●●●

●

●●●●●●●

●●

●

●●●●

●

●●

●

●

●●

●●●●●●●● ●● ●●●

●

●●●

●●●●●●●●●●

●

●

●●

●

●●

●

●

●

●●

● ●●●●●●●

●

●

●●● ●●●●●●●●●●●●●● ●

●

●●

●●●●●

●

●●

●

●●●●●

●●

●

●●

●

●

●

●

●

●● ●●

● ●

●●●●

●

●●

●

● ●●●

●

●●

●

●● ●

●●

●●●

●●●● ●●●●●● ●

●●

●●

●

●

●

●

●

● ●●

●

●

●●

●

●

●●

●●●

●●●●●●

●●

● ●●

●●●●

●

●●● ●

●

●

●

●●

●●

●

●●

●●●●●●●●

●

●●●●●●

●

●

●● ●●●●●●●

●●●●●●●●●●●●●● ●

●●

●● ●●

●

●

●

●

●

● ●●

●●●

●

●

● ●●●●●

●

●●●

●

●●

●●

●

●●●●●●●●●●

●

● ●

●●

●●●●●

●

●● ●●●

●●●

●

●●

●

●●●●

●

●●●●●●

●

●

●

●●●●

●

●●

●

●●

●

●●●●

●

●

●● ●●●●●

●

●●

●●●

●●

●

●

●●● ●

●●

●●●●●

●

●●●● ●●●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●● ●● ●●

●● ● ●● ●●●

●●

●●●

●●●●●

●

●

●●●●

●

●

●●

●

●

●●●●

●●●

●●

●●●●●●●●●●●●●●●● ●● ●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●

●●

●●

●●

●●●●●●●●●●●●

●

●●

●

●

●

●

●

●●

●●

●●●●●

●

●●●●●●● ●●

●

●●

●●●●

●●

●

●

●●

●●

●

●●●

●

●

● ●

●●

●●

●

●●

●●

●

●

●

● ●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●

●

●

●●●

●●

●●●●

●

●●

●

●●●

●

●●

●

●●●● ●●●

●

●

●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●● ●●●●●●●

●

●●●●●●●

●

●●●●

●

●●

●●●

●

●

●●

●●●●●●●

●

●●

●

●●

●

●

● ●●●●

●

●●●● ●

●●●●●●●●●

●

●●●

●

●●●

●

●●●

●

●

●

● ●●●●

●

●

●●●

●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●

●

●●●●●●●●

●●

●●

●●●●

●

● ●●●●●●●

●

●

●

●●●●● ●

●

●

● ●

●

● ●

● ●●

●

●●●●

●

●

●●●●●●●●●

●

●●●●

●

●●●●● ●●●●

●●●●●

●●●●●●●● ●●●●

●●●

●●

●

●●●●●

●●●●●●●●●

● ●

●

●●●●●●●

●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●

●

●●●●

● ●●●

●

●●

●

●●●●●●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●

●

●●

●●

●

●●●●●●●●●●●●●

●

●●

●●

●●

●●●●

●●

●

●●●●●●●●●●●●●

●●

●●

●●●

●●

●●

●

●●●

●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●●●●●●●●●●●

●●●●●●

●

●●●●

●●●●

●●●

●

●●●●●●●●●●

●

●

●

● ●

●

●●●●

●

●●●●●

●

●●●●●●●●●●

●

●

●

●●●●●●●●●●●●●

●

●

●●●●●●●●●

●

●●●●●●●●●●●●●

●

●●●●

●

●

●●

●

●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●

●

●●●●●●●●●●●●●●●●●●●●

●●

●●●

●

●

●●●●●●

●●●●●●●

●●●●

●

●●●●●●

●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●

●●

●●

●●●●●●●●●●●●●●

●

●●●●●●

●●●●● ●●

●●

●●●

●

●●●●●●●●●●●● ●

●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●

●

●●●●●●●●●●●

●

●●●●●●●●●

●

●

●

●●●●●●●

●

●●

●

●●

●

●

●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●

●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●● ●●●●●●●●●●●● ●●

●

●●●●●●●●●●●●●●●●●●●●●

●●

●●●●● ●●●●●●●●●

●

●●●●

●

●●●●●●●●●

●

●

●●

●●

●

●●

●

●

●

●

●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●

●

●

●●

●●●●●

●

●● ●

●

●●●●● ●

●●●●●● ●

●

●

●●●●●●

●●

●●

●●●●

●●

●

●●

●

●

●

●●●●●●●●● ●

●

●●●●●●●●

●●●●●

●

●●

●●●

●

●●●●●●●●●●

●

●

● ●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●

●

● ●●●

●

●●●●

●

●

●

●●●

●●●●●●●

●

●●●●●●●●

●●

●●●●●●●●

●

●

●

●●●●●●●●●

●●●●●●●●●●

●

●● ●●●

●

●●●●

●

●●●●●●

●

●●

●

●●● ●

●

●●●●●

●●

●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●

●●

●●

●●●●●●●●

●●●●●●●● ●●●

●

●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●

●

●

●●●●

●●

●

●●

●●●

●●

●

●

●●

●

●●

●

● ●●●●●●●●●●●●●●●●●●●

●●

●

●●●●●●●●●●●●●●●●●

●●

●●

●●●●

●

●●●●●●●

●

●●●●●● ●●●●●

●●

●●●●●●●●

●

●●●

●●●

●●●

● ●

●●

●

●●●●

●●

●

●

●●●●●●●●●●●●●

●●● ●

●

●

●●●●●●

●

●

●●●●●●●

●●●●●●●●● ●●

●●

●

●●●●

●●●●●●●●●●●

●

●●●●●

●

●

●

●

●●●●●●●●●

●●

●

●

●●●●●●●●●● ●●●

●●

●

●

●●●●●

●●●

●●

●●●●●●●●●●● ●●

●

●●●

●

●●●●●

●●●

●●

●●●

●

●

●●●●

●

●

●

●●●●

●

● ●●●●●●

●

●

●

●

●

●

●●●●●●●●● ●●●

●●●●

●

●

●

●

●

●

●●●●●

● ●

●●●●●

●●

●●●●●●●●

●●

●●●●

●●●

●●

●

●

●●●●●●●●●●●

●

●

●

●●●●●●

●

●●●●●●●●●

●

●●

●

●

●

●

●●●●

●

●

●●● ●●

●

●●

●

●

●●●

●

●

●●

●●

●● ●●●

●

●●●●●●●

●●●

●●●●●●

●

● ●

●

●●

●

●●●●●●●●●●●●●●●●●●

●●

●●●●●

●

●●●

●

●●●●●

●●●●

●

●●●●●●●

●●

●●●

●●●●●●●●●●●●●●

●●●●

●●●●●●●●

●●●●

●

●

●

●●● ●

●●

● ●

●

●

● ●

●

●●●●●●●●

●●

●●

●●●

●●

●

●●● ●●

●●

●

●

●

●●

●●●●

●

●

●

● ●

●●●

●

●●

●●●

●●●

●

●

●●

●

●●

●

●●●●●●

●

●

●●

●●

●

●●●●●●●●●●

●

●

●

●●

●●

●

●

●

●

●

●

●●●●

●

●●●

●

●

●●

●

●●●●●

●

●●

●●●

●

●

●

●

●

●●●●

●

●●

●

●

● ●●●●●●●●

●

●●

●●

●●●●

● ●

● ●●

●●●

●

●●●●

●●

●

●●●●●●

●

●

●

●●●●●●● ● ●●

●

●●●● ●●

●

●●●●

●●●●●●●●●●

●

● ●●●●

●

●●●●●

●

●

●

●●●

●●

●●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●

●

●

●●

●●

●●●●

●●●●●●●●●●●●

●●●

●●

●

●●

●●●

●

●

●

●●

●●

●●●●●

●

●●●

●●●●

●●

● ●●

●

●●

●●●● ●●

●

●●●●●●●

●●●●

●

●

●

●● ●●●●●●

●

●●

●●●●●

●●

●

●

●●

●

●●●●●●●●●●●●

●

●●●●

●●

●

●

●●

●

●●●●●●

●●●

●●

●●●

●●

●●●

●●

●

●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●●

●●

●

●●●●●● ●●●

●●

● ●●●

●●●●

●

● ●●

●●●

●

●

●

●●●●●●

●

●●●

●●●●●

●

●●●

●

●●●●●●●

●●●

●●●●●●●

●●●●●

●●

●●

●●●●

●● ●●●

●

●

●

●●●● ●

●●●● ●

●●

●●●●

●

●●●●●

●

●

●

●

●

●●●

●●●●

●

●

●

●

●

●

●

●

●● ●●

●●

●

●●●●●●●●●●●●

●

●●●

●

●

●●

●

●●●●

●

●●●●

●●

●●●●

●

●● ●●●●●●● ●

●●●

●

●●●●●●●●●●●●●●●●●

●● ●

●●

●

●

●● ●

●

●

●

●●●●●●●

●

●

●●

●●●●●●

●●

●

●

●●●

●●

●●●

●●●●●●●●

●●●●●●●

●●● ●

●

●

● ●●●●●

●●●

●●

●●

●●

●

●

●●●●

●●●●●●

●

●●●●●

●●

●●

●●

●●

●

●

●

●●●●●

●

●●●●●●●●●●●●

●●●●

●●

●●

●●

●●●●●●●●●●●●●

●

●●●

●

●●●●●

●

●● ●●●

● ●

●

●

●

● ●●●●●●

●

●●●●

●

●

●●

●

●●●●

●

●●●●

●●

●●● ●●●

●

●

●●

●

●●●

●●●

●●●●●●●●●●●●●

●●

●●●

●●●●

●

●●

●

●●●●●●●●●●●●●●●●

●

●●●

●●●

●

●

●●

●●

●

●●

●●●

●

●

●● ●

●

●

●

●

●●●●●●●●

●●●

●●●●●●●●●●●●●●●●●●●●●

●●●●●

●

●

●

●●●●●●●

●●

●

●●●●

●●

●

●●●●●●●●●

●●

●●●

●

●

●●

●

●

●

●

●●

●

●

●●

●

●●

●

●●●

●●

●●●

●

●● ● ●

●●

●●●

●

●●●●●

●

●●●●

●

●●●●

●

●●●

●●●

● ●●●

●

●● ●

● ●●●●●

●

●

●

●

●

●

●

●

●● ●●●●●●●●●

●

●●

● ●

●●●

●●●●●●●

● ●● ●●

●●

●●●

●●●

●

●●

●

●●

●●●

●

●●●●

●

●●●●●●

●● ●

●

●

●●●●●

●●

●

●

●●

●

●

●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●

●

●

●

●

●●●●

●

●●●●●

●

●●●

●●

●

●

●●●●

●●●

●

●●

●

●

●

●●●

●

●

●

●●

●

●

●

●

●●

●

●●

●●●●

●

●

●●●●● ●●

●

●

●●●●●●●●

●

●●

●●●

●●●

●

●

●●

●

●

●●●●●●●●

●● ●●●●●●●●

●

●

●

● ●

●

●

●

●

●

●

●●

●● ●

●● ●●

●●● ●●

●

●●●●

●

●

●●●●

●

●

●●●

●●●●●●

●

●●

●●

●

●●

●

●●●●●●●●●●●●●●●●● ●

●●●

●●●●●●●●●●●●●●●●●●

●

●

●●●●●●

●

●●●

●●●●●●●●

●

●●●

●●

●●●●●●●●●

●

●

●

●●

●

●

●

●

●

●●●●●●●●●

●

●●

●

●●●●

●

●●

●

●●

●●●

●●●

●●●●

●

●

●●●●

●

●

●●●

●

●●●●●●●●●

●

●●●●●●

●

●●●●

●

●●

●●

●

●●●●

●●●●●●●

●

●●●

●●●●●

●

●●

●

●●●●●

●

●●●

●

●

●

●●

●●

●●●●●●●●●●●●●●●

●● ●● ● ●

●●●●

●● ● ●

●●

●

●● ●●

●

●

●●●

●●●

●●

●●

●

●

●●●●●

●

●●●● ● ●● ●

●

●●●

●

●

●●

●●●

●●●

●●●●●●●●● ●●●●

●

●

●

●

●●

●

●●●

●●●●●

●●● ●●●

●

●●● ●●●● ●●●●●●●

●●●●●●●

●●

●●●●● ●

●

●

●

●●●

●

●●●●●●

●●

●

●●

●

●

●

● ●●

●

●

●●●●●

●●● ●●

●

●●

●

●

●●

●●

●

●●

●●●●●●●●●●●●●●

●

●

●●

●

●●●

●

●

●

●

●

●● ●●

●

●●●

●

● ●

●

●

●

●

●

●●

●

●●

●●●●

●●

●●●●

● ●

●●●

●●●●●●●●

●●

●

●

●

●●

●●

● ● ●●●●●●●

●

●

●

●●

●

●●

●●●

●●

●

●

● ●●

●

●●●

●

●

●●●●●●●

●

●● ●●●●●

●

●●●●

●

●

●

●

●

●●●

●

●

●

●●●

●●●

●●●

●

●

●

●

●●

●●

●

●

●●●

● ●

●●●

●

●●●●●●●

●●

●

●●●

●●

●●

●●●●●●●●●●●●●●●● ●●●

●

●

●●

●●●●

●

●

●●●●●●●

●●

●

● ●●●●

●

●

●●●

●

●●

●

●●

●●●

●

●●●

●●

●

●●

●●●

●●

●

●●●●●●●●●●

●

●

●

●●●●●●●

●

●

●●

●

●●

●

●●●●

●●

●●●●

●●

● ●●●

●●

●

● ●●●●●

●

●

●●● ●●●●

●●●

●

●

●

● ●

●●●●

●

●●●●

● ●

●

●

●

●● ●

●

●

●●●●

●●

●●

●● ●●

●●●●●●●

●

●

●

●

●●●●● ●●●

●

●●

●●●●●

●

●

●

●●●

●

●

●

● ●●●●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●●

● ●●●

● ●●●

●●

●

●

●

●●

●●

●

●●

●● ●

●

●●●

●

●●

●

●●●●

●●

● ●

●●

●●●●

●

●●●

●●

●●

●●●● ●●

●●●

●●

●●●

●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●

●

● ●

●● ● ●●●

●

●●●●

●

●●

●

● ●

● ●●●

●

●●

●

●●

●●

●●●●●●●●●●●●

● ●●●●

●

●●●

●

●●●●●

●●●●●●

●

●

●

●●●●●●●●

●

●●

●

●

●●●

●

●

●

●●●●●

●

●●●●●●

●

●●●●●

●

●●●

●●

● ●●

●

●●

●

●

●●

●

●●

●●●●●●●●●

●●

●●

●●●

●

●

●

●● ●

●

●

● ●●

●●●

●●●● ●●●

●●●●

●

●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●

●●●●●●●●●●●●●●●●

●

●●●●●●●●●● ●● ●●

●

●

●

●

●

●●

●●●●

●

●●●

●●●

●

●

●●

●

●

●●

●●

●

●●●

●

●●●

●

●

●

●●

●●●●

●

●

●

●●

●

●●●

●●

●●

●

●●● ●●●

●

●

●

●

●●● ●●

●●

●●● ●

●

●●●●●●●●●●●●

● ●●

●

●

●

●

●●

●●

●

●●

●

●

●

●●

●●●●●●●

●

●

●

●

● ●

●●

●●●

●

●●●

●

● ●●

● ●

●

●

●●●

●

●●●●

●

● ●●●

●●●● ●● ●● ●●

●●●●

●

●

●●

●●●●●●●●

●●

●●

●

●●●●●●

●

● ●

●●

●●●●●

●●

●

●

●

●

●

●

●●

●

●●●●

●

●●●

●●●

●●●●●●●● ●●

●●

● ●

●

●

●

●

● ●

●

●●

●●●

●●

●●●●●●●●●●

●●

●

●●

●

●●●●

●●

●

●●

●

● ●●●

●●●●● ●●

●

●●

●●

●

●●●

●

●●●

●●

● ●

●

●

●

●●●●●

●●●● ● ●●

● ●

●●●●

●●●

●

●●

●●●

●●●●

●

●

●●●●●●

●●

●

● ●●

●

●●●●●●●●

●

●

●●●

●

●

●●●

●●

●

●●

●●

●●

●

●●

●

● ●●

●●● ●●●●

●●●

●●

●●●●●●●●●●● ●

●

●●

●●● ●

●

●●● ●

●

●●●●●

●

●

●

●●

●

●●●●

●●

●

●●●

●●●

●

●● ●

●

●●●●●

●●●

●

●

●●●

●

●

●

●

●

●●●●●●●●●●

●

●●●●●●●●●●●●

●●

●

●

●●●●●●●

● ●

●

●●

●

●

●

●

●●●●●●●●●●●

●

● ●●

●●●

●

●●●

●

●●

●●

●●

●●●●

●

●

●

●●

●

●●

●

●●●

●

●●●

●

●●●

●

●

●●●●●●●●●

●

●

●

●

●

●

●

●●● ●

●●

●● ●●●

●

●

●

●●●

●●●●●

● ●

●

●

●

●●●●●

●

●●●●

●

●●●●

●●●

●

●●

●●

●

●●●●●●

●

●

●

●

●

●

●● ●

●●●●

●●

●●●●●●●●●●●●●●●

●

●●●●● ●●

●●

●●

●

●●●●

●●

●

●

●●●●●●●●

●

●●●●

●●●●●

●

●●●● ●●●●

●

●

●●●● ●●●

●

●●●●●●● ●●

●

●

●●●●

●

●

●●●

●●●●

●

●

●●●●●●●●

●

●

●

●●●●●

●

●● ●●

●●

●

●●

● ●●

●●●

●●

●●

●

●

●

●●●●●●●

●●

●

●●●●

●●

●●

●●●

●●●●● ●●●●●●

●

●●●●●●●

●●

●●●

●

●

●● ●●●

●

●●●●

●●

●

●● ●

●

●

●● ●●● ●●●●

●

●

●

●●●

●

●

●

●

●

●●●●●●

●●

●●●●●●●●●●● ●●●

●

●●●

●

●

●●●

●

●●

●

●●●

●

●

●

●

●

●

●●●

●

●●●● ●●● ●●●●

●

●

●●●●●●

●●●

●●●●●

●

● ●●●●●●●●

●●

●

●

●●

●

●●●●●●●

●

●

●

●

●

●●

●●●

●

●●●● ●●●●●●●●●●●●●

●

●

●● ●●●●

●

●●●

●

●●● ●

●●

●

●●

●

● ●●●●●●●

●●●●

●

●●

●

●●●

●●●●

●

●●●●●●● ●●●●●●●●●●●●●●●● ●●●

●

●

●●●

●

●●●

●

●●●

●

●●

●●●●●●●●

●● ●

●●●●●●●●●●

● ●●●●●●●●●●●

●

●

●

●

●

●

●

●

●

●

●

●

●

●

●●

●●●●●●

●●

●

●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●

●●●●●

●

●

●●●●●●●

●●●●

● ●●●●●●●●

●

●●●

●●

●●

●

●●●

●

●

●

●●●●

●

●●●●●●●●●

●

●

●●●

●

●●●●●●●●●●●

●●●●●●●

●●●●● ●●●●●

● ●●●●

●

●

●

●●●●●

●●●

●●

●●

●●

●●●●

●●●

●

●●●●●●●●●●●

●

●●●●●●●

●●

● ●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●

●

●

●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●

●

●●●●● ●

●

●● ●●●●●●●●●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●

●●

●

●

●

●

●

●

●●●

●●

●●●

●

●●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●● ●●●

●

●●●●●

●●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●● ●

●●

●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●

●

●

●

●●●

●

●● ●● ●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●●●●●●●●

●

●●●●●

●

●●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●

●●

●

●

●

●●●

●

●

●●●

●●

●

●●●●●●●●●●●●●●

●

●●

●

●●●●

●●●

●●●

●●

●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●●●●

●

●●●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●

●

● ●●

●

●

●●

●

●●

●●●●

●●

●●●●●●

●

●●●●●●●●

●

●

●●●●●●●●●●●●●●●

●

●●●

●●●

●●

●●

●

●

●●

●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●

●

●

●

●

●●

●●

●●●●●

●

●●●

●●

●●● ●●

●

●●●

●

● ●●

●

●●

●

●●

●

●●

●

●●

●

●● ●

●●●●●●●

●

●●●

●

●

●

●●●●●●

●●

●●●●●●●●●●●●●● ●

●●●●●●●

●●

●●●

● ●

●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●

●●●●

●●

●●●●●●●●●●●●

●

●●●●●●● ●●

●●

●

●

●

●●●●●●●●●●

●

●●●●●

●

●

●●

●●●●

●

●●●●●

●

●●●●●●●●●●●●● ●●●●●

●●

●

●

●

●●●●●● ●●●●●●●●●●●●●

●

●●●●●●●● ●●

●

●●●●

●●●●●●●●●●●●●●

●

●●●●●● ●●●

●

●

●

●

●●●●●●

●●

●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●

●

●●

●●

●●●●●●●●●

●

●●●●●

●●●●●●●●●

●

●●●

●●●

●

●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●

●●●●●●●●●●●●●

●●

●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●

●

●●●●●●●●●●●●●●●●● ●●●●●●●●●●

●●●

●●●●●●●●

● ●●

● ●●

●●●

●●●●●●●

●

●●

●

●●●●●

●

●●●●●●●●●●●●●

●●●●● ●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●●●

●●●

●●●●●●●●●●

●

●●●●

●

●

●●●●●●●●●●●●●●

●

●

●

●●

●●●

●●●●●●●●●

●●●●●●●●

●

●●●●●●●●●●●●●●●

●●

●●●●

●●

●

● ●●●

●

●● ●●●

●●●●●●●●●●●●●●

●●

●

●●●●●●● ●●●●●●●● ●

●

●●●●●●●●●●●●●●●

●

●●●●

●●●●

●●●●●●●●●●●●●●● ●●●●

●

●

●●

●●●

●

●●

●●●●

●●●●

● ●●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●●

●

●

●●●●●

●●●●●●●●●●

●●●●●●●

●● ●●●

●

●

●

●

●

●●●●●●●●●●●●●

●

●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●

●●●●●●●●●●●

●

●●●●●●●●●

●

●

●

●

●●●●●

●●●

●●

●

●●●●●●●

●

●●

●

●●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●

●●●●

●●●●●●●●

●

●●●●●●

●

●●●●

●●●●

●

●●●●●

●

●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●

●

● ●

●

●●●● ●●●●●●●●●●●●●●●●●

●

●

●●●●

●

●● ●●●

●

●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●

●

●

●

●

●

●

●●●●●●●

●

●

●

●●

●

●

●

●

●●●

●

●●● ●●●

●●

●●●●

●

●●

●●

●

●●●●●●●●●●●●

●

●

●

●●

●●●●●

●●

●●●

●

●●●●

●●●●

●

●●●●●● ●●●●●●

●

●

●

●●●●●●●● ●●●●●●●

●●

●●●

●●●●●●

●

●●

●

●

●● ●●● ●●●●●

●

●●

●

●

●●

●

●

●●●●●

●●●●●

●

●●

●●●●●●

●

●●●●●●

●●

●●

●●

●●●

●

●●●●●●

●●

●●

●

●

●

●●

●●●

●

● ● ●

●

●●●●●●●●●●●

●

●●●●

●●

●●

●

● ●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●●●●●● ●●●●●●●●

●

● ●●●

●

●

●●●●●●●●

●

●●●

●●●

●

●●●●●●●

●

●

●

●●●●●●●● ●

●●

●●●●

●

●

● ●●

●

●●●●●●●●●●●●●● ●●●

●●

●

●●●●●●

●

●●●●●●

●

●

● ●●●●●●●●●●●

●●

● ●●●●●●●●

●●●

●●●●●●●●

● ●

●●●●●

●●●●●

●● ●●

●●●

●

●

●●

● ●●

●●● ●●●●●●●●

● ●●●●● ●●●●●●●●●●● ●

●

●

● ●●●

●

●

●

●●●●●●●●●●● ●

●●●●

●

●

●

●

●

●

●●●●●

●

● ● ●●

●

●●●●●●●●●●● ●

●●●

●●●

●

●●

●

●●●●

●●●

●

●

●●●●●

●●●

●

●●●

●

●●●●

●●●●

●●●● ●

●

●

● ●

●●

●

●●●●●●●

●

●

●

●●●●●●●●●●●

●

●

●

●●●●

●●●

●●●●●●●

●

●

●

●●●●●●●●

●

●●●●●●●●●●●

●

●●● ●

●

●

●

●

●●

●● ●

●

●

●●

●

●●●●●●●●●

●●

●

●

●●●●

●

●

●

●●●●●

●●●

●

●

●

●

●●

●

●

●●●

●

●●

●●●

●

●●●● ●●●●●●●●●●

●●

●

●●●

●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●● ●●●●●●●●●

●●●

●●●●●●●●

●●●●●●●●

●●

●●

●●

●

●

●●

●

●

●

●● ●●●●●●●●●●●●●●●

●

●

●

●●●●

●

●●●

●●

●

●●●●●

●●●

●●●●●●

●

●●

●

●●●●●●●●●

●

●●●●●●●●●●●●●

●●●

●

● ●● ●●●

●●

●

●●

● ●●●●

●

●

●

●

●●●●●

●

●

●

●

●●●

●●

●

●

●● ●

●●●●

●

●●

●●

●

●●

●●

●

●●

●

●

● ●

●

●

●●

●

●

●●●●

●●

●●

●●

●

●●●● ●●●

●

●●

●●

●●●

●

●●●●●●

●

●●●●●

●●●

●●

●●●

●

●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●

●

●● ●

●●●●●●●●●

●●

●● ●●●

●

●

●●●

●

●●

● ●

●●

●

●● ●

●●●●

●

●

●

●

●

●●●●●●●

●●

●

●

●●●

●

●

●

●

● ●●●●

●● ●

●

●

●●

●

●

●●●

●●

●

●

●●●

●●●●● ●●

●●●●● ●●

●●

●●●

●

●●●●●●●● ●● ●●●●

●

●●

●●●

●●

●

●●

●

●●●●●●

●

●

●

●

●

●

●

●●●

●● ● ●●●

●

●● ●

●

●●

●

●●●●● ●●●

●

●●

●●●

●

●

●●●

●●

●● ●●●

●●●●

●

●●

●●

●●

●

●●●●●

●

●●●●●

●

● ●

●●●●●●

●

●

●●

●

●●●

●

●●

●●●●●●

●● ●

●●●●● ●

●●●

●●●

●●●

●● ●

●● ●●●●

●●●

●●●●●●●●●

●

●●

●●

●

●●●●

●●●●●

●●

●

●

●

●●●●●●

●●

●●

●

●

●

●●

●●●

●●●●●●●●●●●

●

●●●● ●

●

●

●

●●●●

●

●

●●●

●●

●

●

●

●

●●●●●

●●●●●

●

●

●

●●●

●

●

●

●●●●● ●●

●

●●●●●●●

●

●

●

●●●●●

●●●

●●

●

●●●●●●●●●●

●

●

●●

●

●

●●

●

●●●

●

●●●●

●

●

●

●

●

●●●

●●●●●●●●●●●●●●

●●

●

●

●

●

●●●

●

●

●●

●●

●

●●●●

●

●

●●●

●

●

●

●●●●

●●●

●●

●●

●

●●●●●●●

●●●●●

●●

●●●

●

●●●●●●●●●●●● ●●

●

●●●

●

●●●

●● ●

●●

●●

●●

●●

●

● ●

●

●●●●●●

●●●

●●●●● ●●●

●●●

●

●●●●●●●●●

●●●

●●●● ●

●

●●

●

●●●

●

●●● ●●●●●●●●●●

●

●

●

●

● ●●

●

●●

●

●●

●

●

●●●●●

●

●●

●

●

●

●

●●

●●

●●●

●

●●

●●●

●●

●●

●●●

●

●

● ●●

●●

●●

●●●

●

●

●

●●●

●●

●● ●

●

●

●

●●●

●●

●

●●●●●●

●●

●

●

●

●●

●

●

●●●●

●●

●

●●●●●● ●●

●●

●

●●●

● ●●

●

●

●●

●

●●●

●

● ● ●●

●

●● ●●●●●●● ●●● ●

●●●

●

●

●●●●●

●

●●

●

●●

●●●●●

●●

●

●

●

●●●●●●●

●●●

●●●●●

●●

●

●

●●●●

●●●

●●●

●

●

●

●

●

●

●

●

●

●●●

●●●

●

●

●●

●●

●

●

●●● ●

●

●●

●

●

●

●

●●●

●●

●

●

●●●●

●

● ●●●●●●●

●●

●●●

●●

●●●●

●●●

●

●

●

●

●●●●●●●

●

●

●● ●

●

●●●●●●●

● ●

●●●●●●●●

●

●●●●●●●●●●●●●●●

●●●

●●●●●●●●

●

●●●●●●

●

●●

●

●

●●●●●●●●●●●●● ●●

●●

●

●●

●

●

●●

●

●

●

●●

●●

●

●●●●●●●● ●●●●●●●●●●●●

●

●●

●●

●

●

●●●●●

●● ●

●

●

●●●●●●●●● ●●●●●●●●●●●

●

●●●●

●

●●

●●

●●

●

●

●

● ●●●●●●●● ●

●

●

●●

●●

●

●●

●●●

●●

●

●●

●

●

●●

●

●

●

●

●●

●●

●

●●●

●

●

●

●●●●●●●

●

●●●●●

●

●

●

●

●●

●●

●●●

●

●

●

●●

●

●●●●

●

●

●

●●●●●

●

●●

●

●●●●●

●

●

●

●●●●●●●●●

●

●

●

●●●●

●

● ●

●

●●

●

●

●

●●●●●●●●

● ●

●●●

●

●

●

●●●●●●●●●●

●●

●

●●

●

●

●

●● ●●●●●●●●●●●●●●●●●●●●●● ●●●●● ●●●

●● ●

●

●●●●●●●●●

●●

●●

●●●●●●●●●●●●●

●

●

●

●●●

●

●●●

●

●●●●●●●●●●

●●

●●●

●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●●

●

●●

●

●●●●

●

●●●

●●

●

●

●●●●●●●●

●●

●

●●

●●

●

●

●●

●

●

●●●●●

●

●

●

●●●●●●●

●

●

●

●●

●

●

●

●●

●●●●●●

●

●●●●●●●●● ●

●

●●

●●● ●●●●●

●●●●●●●●●

●

●●●●●●●●●●

●●

●

●

●●

●

●

●

●●●●

●●●●

●

●

●●●●

●

●

●●●

●

●●●

●●

●

●

●

●●

●●●●

●●●●●●●●

●●

●

●●●

●●●

●

●

●●●

●

●●

●

●● ●●

●

●●

●

●●●

● ●●

● ●●

●

●●●●●●

●

●●

●

●● ●●

●●●

●●

●

●

●

● ●

●

●●●●●●●●●●

●●●●●

●

●

●●

●●●●● ●

●●

●●

●●

●●

●

●●●

●

●●

●●

●

●

●

●

●●●●●●

●

●

●

●●●●● ●

●

●●●

●

●

●

●●

●●

●

●●●

●

●

●●

●● ●●

●●●

●

●●

●

●●●

●

●

●

●

●●●●

●●● ●

●

●

●

●●

●●●●●

●●

●

●

●

●

●●

●●●●●●●●●●●●●●●●●

●

●●●●

●

●●

●

●

●●

●

● ●●

●

●●

●●●

●●●●

●

●

●●●●●

●

●●

●

●

●

●

●

●●●●

●●

●●●

●●●●●●●●●

●

●●●●

●●●●●●●●●

●

●

●

●

●

●●●●

●

●

●

●

●

●●

●●●● ●●●●●●●●

● ●●●●

●

●●●●

●

●●●

●●

●

●●●●●●●●●●●●●●●

●

●

●

●●●●●

●

●

●

●●●●●

●

●●●●●●●

●●

●

●

●●●●●●●●●●

●●

●

●●●●

●●●

●●●●

●

●●●

●

●●

● ●

●●

●

●●

●

●

●

● ●●●●●●●●●●

●

●●

●

●●●●

●

●● ●

●●

●

●●●

●●

●●

●●

●

●

●

●

●●

●

●

●

●●●

●● ●●

●

●●

●●●

●●●●

●

● ●●

●

●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●

●●●●●●●

●

●●●●●

●●●●●●●●●●●●●

●

●●

●●

●

●●

●●●

●●

●●

●

●●●●●●●●

●

●●● ●●●●●

●

●●●●●●

●

●●●●

●

●

●

●●

●

●●●●●●●●●●●●

●

●●●●●●●

●

●●●

●

●

●

●●●●

●● ●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●●

●

●

●●●●●●●●●●●●●●

●

●●●●●

●●

●

●●●●●●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●

●●●●●

●

●●●●●●●●●●●●●●

●●●●●

●●●●● ●●

●●

●●

●●●●●●●●●●

● ●●

●●●

●

●●●

●●

●●●●●●●●●●●●●●●●

●●● ●●

●●●

●

●●

●●

●

●

●●●●●●●●

●

●●

●

●●●●●●●●

●

●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●

●●

●●●●●●●●●

●

●●●●●●●●●●●●●●●

●

●

●●●●●

●●

●●●●●●●●●

●

●●●

●

●●●

●

●

●●

●

●●●●●●●●●●●●●●●●

●

●●●●●●

●●

●●●●● ●

●

●●●●●●●

●

●

●●●●●●

●

●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●

●

●

●

●●●●●●●●●●●●●●

●

●●●●●●●●

●●●

●●

●●●

●

●●●●●

●● ●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●

●●

●●●●

●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●

●

●●

●

●

●●

●

●● ●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●

●

●

●

●●

●

●● ●

●

●

●●●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●

●●

●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●●●

●

●

●●●●●●●●●●

●

●

● ●

●

●

●

●

●

●●●

●●●●●●

●●

●●●●●

●

●●●●●●● ●

●●

●●●●●

●●●●●●●●●

●

●●●●●●●●

●●●

●

●

●

●

●●●●●●●●●●●●●●●●

●

●●●●●

●●

● ●●●●

●

●●

●●●●●

●

●●●

●●

●

●●

●●●●●

●●

●

●

●●●

●●●

●

●●●●●●

●●

●●●●

●

●

●

●●●

●●●

●

● ●●

●●

●●

●●●●●●●●●●

●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

● ●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●●

●

●

●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●

●

●●

●●

●●●

●

● ●●●●●●●●●●●●●●● ●●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●●●●●●●●●

●

●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●●● ●●●●

●

●●

●

●●●●●●●

●

●●●●

●●

●●●●●

●

●●●●●●●●●●●●●●●●●● ●

●●

●

●●

●

●●●●●●●●●●●●

●

●●

●●●●●●

●●●

●

●

●●●●●●●●●●●●●

●

●●

●

●

●

●

●

●●●

●

●

●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

● ●●

●

●

●●

●●●●●●

●●●●●●

●

●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●● ●

●

●●●●●●●●●●

●

●●● ●

● ●

●

●●●●●●●●●●●●●●

●●

●

●

●●●

●

●●●●●●●●●●●●●●

●●

● ●●

● ●

●●●

●

●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●

●

●●●●

●

●●

●

●●

●● ●

●●

●●

●●●●

●●●

●

●●●●●●●●●●●●

●●

●

●●

●● ●

●●●●●●●●●●●●●●

●

● ●

●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●● ●●● ●●

● ●●

●

●

● ●

●●

●●

●●

●

●●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●●● ●●

●●

●●●●●●●●

●●

●●

●●

●

●●●●●●●●●●●●●

●

●●●●●

●●●●●●

●

● ●

●●

●●

●

●

●●●●●●

●●●

●

●

●●

●●● ●

● ●●●

●●

● ●

●●●

●●

●●●

●

●

●●●●

●

●

●

●

●

●●

●

●●●

●

●●●

●

● ●●

●

●●●●●●●●●●

●

●●●●●●●●

●●●●●●

●●

●●●●●

●●

●●●●●

●

●●●●●●

●

●●

●●

●

●

●●●

●

● ●●

●

●● ●

●●●

●●

●●●●

●

●

●●

●●●●●●●

●●

●

●●●●

●

●●

●

●●●

●●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●●●●●●●●

●

●

●

●

●

●●

●

●

● ● ●

●●

●●

● ●●●●●●●●●●●

●●●

●●●

●●●●●●●

●

●

●

●●●●

●

●●●●

●

●●

●

●

●

●

●●●●●●

●

●

●●●● ●

●●●

●●

●

●

●●

●●●

●●●●

●

●●●●●●●●●●●

●

●●●●

●

●

●

●

●

●●●●●●●●

●

●

●

●

●

●●●●●●●●●●

●●●

●●●●

●●

●●●●●●●●

●●

●●●●●

●●●

●

●●●●

●

●

●●

●●●●●●●●

●

●●●●●●●●●

●●

●

●●

●

●●●●●●●●●●●●●

●●

●

●

●

●

●●●●●●●

●●

● ●

●

●

●●

●

●●

● ●●●●●

●

●●

●

●

●●●●●●

●

●●

●●

●

●●

●

●

●●●

●

●●

●●●

●

●●●

●

● ●●

●●

●●●●

●

●

●

●●●● ●●

●

●●●

●●

●●●●●●●●●●●●●●●●●●●●

●●●

●

●●

●●●● ●

●

●

●●●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●● ●●

●

●

●●

●

●

●●●●

●●

●●

●

●

●●

●●●●●●●

●

●●●●●●●●

●

●●●

●

●●●●●●●●●●●●●

●●●●●●●●●●●●●

●

●

●●●●●● ●●

●

●

●●

●●●●●

●●●

●

●

●

●●

●

●●●●

●

●●●●

●

●

●

●●●●●

●●●●●●●●●

●

●● ●●

●●

●●●

●●

●

●

●●●

●

●●●●●

●

● ●

●●

●●●●

●

●

●●

●

●●●●●

●

●●●●●●

●

●

●●●

●●●●●●●

●

●

●● ●●

●●●

●●●●

●

●

●●●

●

●

●

●

●●●●

● ●●●

●●●●●●●●

●

●

●

●

●

●

●

●

●

●

●●

●

●●●●

●●

●●

● ●

●

●

●

●

●●●●

●

●●●●●●

●

●●

●

●●●

●

●●●

●

●● ●

●●●●●●●●●●●

●●

●

●

●●

●

● ●●●

●

●●

●

●●●●● ●

●●●●●●

●

●

●●

●●●●

● ●

●

●

●●

●●●●

●

●●●

●

●●●●

●●● ●●●●

●●

●

●●

●● ●●●●●

●●● ●●

●●●●● ●

●

●●

●

●●

●

●●●●●●

●●●

●●

●

●● ●●

●

●●●

●

●

●

●

●

●●●●●

●

●

●● ●●

●

●

●●●●●●●●

●

●●●●●●●●●

●

●● ● ●●

●●

●

●

●

●

●●●●●●●●●

●

●

●●

●●●●●

●●●

●●

●

●

●

●●●●● ● ●●●●●●

●●

●●

●●●

●

●

● ●●

●●

●

●●●●●●●●●

●

●●●

●

●

●

●●●●

●●●●●

●

●

●●

●●●

●

●●●●

●●

●

●●

●

●

●●

●

●

●●●

●●

●

●

●

●

●

●

●

●●●

●●

●●●

●

●●●●●●●●●●

●●

●●●●●●●●●●●

●●●●

●●

● ●

●

●●

●●

●●● ●

●●●

●

●●●

●

●

● ● ●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●

●

●●

●

●

●●

●●

●

●●

●

●●

●●●

●●●

●

●●

● ●

●●

●

●●

●

●

●

●

●

●●●●

● ●

●

●●●●●●●

●●●●●●

● ●●

●●

●●

●●●●

●●●●

●●

●

●

●●

●

●●●

●●

●●●●●●●

●

●

●

●

●●●

●●● ●●

●●

●● ●

● ●●●

●

●

●

●

●

●

●●

●●

●●●●●

●

●●●●● ● ●

●●●●●●

●

●●●

●

●●

●● ●

●

●

●● ●

●

● ●

●

●● ●●●●●

●

●●●

●

●●

●

●●

●

●

●

●●●●●

●

●●●●

●●

●●●●

●

●●●

●

●●●●●

●●●

●

●

●●

●

● ●●●●

●●

●

●

●●●

●

●●

●

●●

●●●●●●●

●

●

●

● ●

●

●

●

●

●●

●●●● ●● ●

●●

●●●

●

●

●●●●●

●

●

●●

●

●●●●●●●●●●●●●●●

●●●

●●

●

●

●

●

●●●●●

●

●●

●

●

●●

●● ●●●

●●

●

●●

●●

●

●●●

●

●

●

●●

●

●●

●

●●●●●●

●●●

●

●

●

●●●●●●

●●

● ●

●●●●

●

●

●

●

●●

●●

●● ●●

●

● ●●●●

●

●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●

●●●●●●●

●

●

●

●●●●

●

●

●●●

●●●

●●●●

●●

●●●

●

● ●

●

●

●

●

●

●

●● ●

●●

●

●

●●●●●

●

●

●

●

●●●●

●●

● ●●

●

●●

●●

●

●●●●

●

●

●

●

●●

●●

●

●● ●

●●

●●●

●●●●

●

●

●●●●●●

●●●

●

●

●

●

●●

●

●

●●●

●

●

●●●●●

●●●●

●●●

●

●●●●

●

●●●

●

●

●

●

●●●●●●●

●●

●

●●

●

●●●●●

●●

●

●

●●

●

●●●●●●

●●●

●●●●

●

●

●

●

●

●●●

●●

●●●●●●●

●

●

● ●

●●●

●●

●●●●

●● ●

●●●

● ●●

●

●●

●● ●

●

●

●

●●

●

●

●●

●●●

●●

●

●●

●●●● ●●

●●●

●●

●●●●●●●●●

●

●●●●

●

●

●●●

●●

●●

●●

●●

●

●

●●

●●

●

●●●

●●

●

●

●

●

●●●●

●●●●●

●●

● ●

●●

●

●●●

●●

●●

●

●●

●

●●

●●●

●●● ●●

●

●●

●●●

●

●

●

●●●

●

●●

●●●●

●●●●

●●

●

●●●

●

● ●

●

●●●●

●●●●

●

●

●

●

●

●●

●

●●●

●●

●●●●

●

●●●

●● ●

●●

●●

●●

●

●●●●●●

●

●●●● ●●●●●

●

●●

●

●●●

●● ●

●●●

●●●

●

●●

●●

●

●

●●

●

●●●●

●●●

●●●

●●

●●

●● ●

●●

●●●

●●●●● ●

●●●

●●

●

●

●●

● ●

●●●

●

●●●

●●●

●

●●●●●●

●

●●

●●

●

●●● ● ●

●●●● ●

●●

●

●

●

●

●

●

●

●

●●

●●●●

●

●

●● ●●

●

●●●

●

● ●●

●

●●●

●●●

●

●

●●

●●●●●● ●

●●

●●●

●

●●●●●●

●

●

●●

●●

●●●

●

●

●●●

●●●●●

●●

●

●

●

●

●

●●●

●

●

●●

●●●●

●

●

●●●●●

●

●●●●●●●●● ●

●●

●●● ●●●●

● ●

●●●

●●

●●

●

●●

●

●

●●●●

●

●

●

●

●● ●●

●●

●●

● ●

●

●

●●●●●●●●●●●●●

●

●

●●●●

●

●●●●

●

●●●●

●

●●●

●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●

●●●●

●●●●●●●●●●

●

● ●

●●

●

●●●●●

●

●●

●

●●●●●●

●

●● ●●● ●

● ●

●

●

●

●●

●●

● ●●●●●●●●●●●●●●●●●●●●

●●

●●

●●

●

●●●●●●●●●●

●

●●●●●●●●●●●

●

●●●

●●●

●● ●● ● ●●●

●

●

●

●●●●●

●

● ●

●●

●

●●

●

●

●●

●

●

●●

●

●

●

●●

●

●●

●

●●●●●●●

●

●

●

●●●●●

●

●

●●●●●●●●●●●●●●

●●

●

●●

●●

●●

●●●●●●●

●

●●●●●

●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●

●

●

●●●●●●●●

●

●●

●

●

●

● ●●●

●

●

●

●

●●

●●●●●

●

●

●● ●

●

●●●●●●

●●●●●●●

●●

●

●

●●

●

●

●●

●

●

●●● ●●

●

●●

●●●

●

●

●● ●●

●

●●●●

● ●

●●●

●●

●

●●

●

●

●

●

●●

●●

● ●●●●●●

●●●●●●●●

●●

●●●

●●●●

●●●

●●●

● ●●

●

●

●

●●●● ●●

●●

●

●●●●●

●

●

●●

●●●●●●●●●●●●●

●

●●●●●●●

●

●●●●●

●●

●●●●●●●●●●●●

●

●

●

●●●●●●●●●●

●

●●●

●

●

●●

●

●

●●●●●●●

●●

●●

●●●●

●

●●●●●

●●

●

●●●●●

●●

●●

●

●●

●

●●●●● ●●●

●

●●● ●●

● ●

●●

●●

●●●●●●●●●

●

● ●●

●

●● ●

●●●

●●● ●

●

●

●

●●● ●●● ●●●● ●

●●●

●

●●●●●●●●●●

●

●●●●●

●

●

●

●●●

●●●●●●

●

●●

●

●●

●●

●●●●●●

●●

●●●●●

●

●●●●●●●●●●●

●●

●

●●

●

●

●●●●●

●

●●●

●

●

●●

●●●

●

●

●

●

●●

●●

●●●●●●●●

●

●

●

●

●●●

●●

●

● ●●

●

●

●●

●

●

●

●

●●

●

●●●●●●●●●●●●●●●●●

●●●●●●

●

●●●●●

●

●●

●●●

●●●●●● ●

●●●●●●●●

●

●●

●●●

●

●●

●●

●

●

●

●●●●●

●●

●●

●●

●●●

●●

●

●●●●

●

●

●

●●●●●●●●

●

●

●●●

●

●

●

●●●

●●

●

●

●

●

●●●●

●

●●

●

●

●

●

●●

●

●

●

●●

●●●●

●

●●

●●

●●●

●

●

●●

●●

●

●

●●●●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●● ●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●● ●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●●●●●●●●●●●● ●●●●

●

●●●●●●●●●●●●●●●●● ●●●●● ●●

●●●●●●●●●●●●●●●●●● ●●●●●

●

●●

●● ●

●●

●

●●●●

●

● ●●

●●

●

●●●●●●●●●

●

●●●●●

●

●

●●●●

●●

●

●●●●●●●●●●●●●

●

●●●●●

●

●

●

●

●

●●●●●●●

●

●●

●

●

●

●●●●●●●●●●●● ● ●●●●●●●●

●

●●●●●●●●●●●●●

●

●●

●

●●

●●●

●●●●●●●●

●●●● ●● ●

●●

●

●●●●●●●

●

●

●●●

●

●●●●●

●

●●●● ●●●

●●●●●●●●●●●●●●●

●

●●

●

●

●

●

●

●●●●●

●

●●

●●

● ●●●

●●●●●

●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●●●●●●

●●●●●●●●●

●●

●●● ●●●●●●●●●●●●●●●●●●●

●●●●

●●●●●●●●●●●●●●● ●

●

●●● ●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●

●●●

●●●●●●● ●●

●

●●

●

●●●●●●●●●●●

●●●●●

●●

●

●

●

●●●

●●●

●

●● ●●

● ●●

●●●●●●●●●●●●

●

●

●

●●●●●

●

●

●●●●●●●●●●

●●

●●●●●

●

● ● ●●●●●●●●●●

●

●●●●●●●●●

●●●

●●●●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●

●●

●

●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●●

●

●●●

●●●

●

●●●●●●●●●●

●●●

●●●● ●●

●

●●●●●●●●●● ●●●●●●●●●●

●●●

●●

● ●●●●●

●●

●

●

●●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●

●

●

●

●

●●

●●●●●●●

●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●

●

●●●●●●●●●

●

●●

●

●●

●

●●

●

● ●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●

●

●●

●

●●

●●●

●●

●●●●

●

●●●●

●●●●●●●●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●●●●●●●●●● ●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●

●

● ●●●

●

●●

●

●●●●●●●

●●●

●

●●●●

●●●●●●

●●

● ●●●●●●●●●●●●●●●●●●●●●●● ●

●●

● ●

●

●

●

●●●●●●

●

●●

●●

●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●●●●●●●●●

●

●●●●●●●

●

●●● ●● ●●●●●●●●●●●●●●●●●●●

●

●●●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●

●●

●●●●

●

●●●●

●●

●●●

●●

●●● ●● ●

●●●●●●●●●●●●●●●

●●●●●

●

●

●

●●

●●

●● ●●●●●●●●●●

●

●

●

●●

●

●●● ●

●●●●●●

●

● ●

●●

●●●●

●

●

●●

●●

●

●●●●

●

●●●●●

●

●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●

●

●●●●●●●●

●●

●

●●●●●●●●●●

●

●

●●●●●●●

●●

●

●

●●●●●●●●●●●●

●

●

●●

●

●

●

●

●

●

●●●●

●●

●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●● ●

●

●●●●

●

●●●●●●●●

●

●

●●

●●●●●●

●

●●

●●●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●●

●●●●●

●

●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

● ●●

●

●

●●●●

●●

●●●●●

●●

●●●●●●●●●●●

●

●●

●●

●

●●●●●●●●

●

●

●

●

●

●●

●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●

●●

●●

●●

●●●●

●●

●

●

●

●●●

●●●●●●

● ●●

●●

●

● ●●●

●●●

●●

●

●●●●●●●●●●

●

●●● ●●●●●●

●●●●●●

●

●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●● ●

●

●●

●●●

●

●●●●●●●●●●●●●●●●●

●

●

●●

●

●

●●●

● ●●

●●●●●●●

●●

●●●●●●

●

●●●●●●●●

●

●

●●●

●

●●●●●●●

●●●

●

●●

●

●●●●●●●●●●●●●

●●●●●●●●●

●

●●

●●●

●

●●●●

●

●

●●●●

●

●

●●●●●●

●●●●

●

●● ●● ●

●●

●

● ●●

●

●● ●●

●

●

● ●●●●

●●

●

●

●

●●

●●●●●●●●

●

●

●●

●

●●

●

● ●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●

●●

●

●●●●

●●●● ●●●●

●●●

●

●

●

●●●●●●●●●●●●●●

●●

●●

●

●●●●

●●

●●

●

●

●●

●●●●●●●

●

●●●

●● ●

●

●●●

●●

● ●

●

●●

●●

●

●

●●●●●●●●●●

●●

●

●●

●●

●●

●

●●

● ●●

●

●

●

●●●●●●●●●●

●●●●●●●● ●●●

●●●●

●

●

●● ●

●

●

●

●

●

●●●●●●

●

●●●●●

●●● ●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●

●

●

●●

●

●

● ●●

●●●

●●●● ●● ●

●●

●●●●

●●●●●●●●●●●

●

●

●

●●●●●●●●●

●●

●●●●● ●

●●

●●●●●●

●

●

●

●●● ● ●

●●●●●●●●

●

●

●●●●●●

●

●●

●

●

● ●●●●●●

●●●

●●

●●●●●●●●●●●

●●●●

●

● ●

●

●

●●

●●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●

●●●●●●●●

● ●

●

●

●●●

●

●●●●●●●●●●

●●

●●●●●●●●●●●●

● ●

●

●

●

●●

●●

●●●●

●●

●●●●●●●

●

●●●●●●●●●

●

●●●

●

●●●●●●●●●●●

●

●●●

●●

●●●●●●●

●

●●●●

●

●●

●

●●

●

●

●

●●●●●●●

●

●

●●●

●

●●●●●●●●●●●●●●

●

●●●●●●

●●●●●●●

●●●

●

●●●● ●● ●

●●●●●● ●●●●

●●

●●

●

●●●

●

●

●●

●

●●●

●●

●

●

●●

●

●●●

●

●●

●

●

●●●● ●●●●●●●●

●●

●

●● ●

●

●●●●●●●

●

●●

●●●

●●●●

●

●

●

●●

●

●●●

●

●●

●●

●●

●

●

●●

●●

●●●●● ●●●

●●

●

●●

●●

●●●●

●

●●

●

●●●●●●●●●●●●

●●

●●

●

●●●●

●●●●●●●●●●● ● ●

●

●

●

●●

●

●●●●

●

●●●●●●●●●●

●●

●●●●

● ●●●●●●●●●●●

●

●●

●

● ●●●●●●●●●

●●

●

●

●●●● ●●●●●

●

●

●●●●●●

●●●

●●●

●●●●●

●●

●

●

●●●

●

●

●

●●●●●●●●

●●

●●

●

●●

●

●●

●

●●●

●

●

●

●●● ●●

●

●●

●

●●●●●●

●●●● ●

●

●

● ●●

●●

●●●

● ●

●●● ●

●●●●●●

●

●●●●●

●

●●●●

●●●

●●●●●

●●●

●●

●

●

●●●●●●●

●

●

●● ●

●●●

●

●●

● ●

●●●●●●●

●●

●

●

●●●●●●●●

●

●●

● ●●

●

●

●

●

●●

●

●●

● ●

●●●

●

●

●●

●

●●●●●●●●●●●●●●

● ●●●

●

●

●●

●●●

●●● ●●

●

●

●

● ●

●●

●

●

● ●●●●●●●●

●

●

●●●●●●●●●●●●

●●●●●●

●● ●

●●

●

● ●●●●

●●●

●

●

●●●●●● ●

●●●●

●

●

●●

●

●●●

●

●

●●●●●●●●●●

●●●

●●●●

●●

● ●●

●

●

●

●●

●

●●●●

●●●

● ●●

●

●

●

●●●

●●● ●●

●

● ●●●●●●●●●

●

●●●●

●

●

●

●●● ●●

●

●

●

●●

●●

●

●

● ●

●

●

●

●●●

●●

●

●

●●●●●●●

●

●●●●

●

●

●

●●●●

●

●●●●●●●● ●

●●

●

●●●●● ●

●

●●

●

●

●●●● ●●●

●

●●● ●

●●

●●●

●●●

● ●

●●●

●●●●

●

●●●●●●

●●

●

●

●●

●●

●● ●

●●

●

●

●●●●●●●● ●

●

●●●●● ●

●

●

●●●

●

●●●

●

●

●

●●

●

●

●●

●

●●

●●

●●

●●●

●

●●

●

●

●●●●

●●

●

●●

●

●●●

●

●●

●●

●

●

●●●●●●●●

●

●

●

●

●

● ● ●●

●

●●●●●●●●

●

●

●●●●

●●

●

●

●

●● ● ●

●● ●

●

●●

●●

●

●●

●

●●

●

●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●●

●

● ●●

●●

●●●●●●●

●

●●●●●●●●●●●●

●

●

●●●●

●●●

● ●

●●●

●●●

●

●

●

●

●●

●●

●●

●●

●

●● ●●●

●●

●●

●

●

●

●

●

●●●●

● ●

●

●●

●

●●

●●

●●

●●●

●

●●

●

●●●●●●●●●●

●●

●●●

●●●●●●

●

●

●

●●●●●●●●

●

●

●

●●●●●

●

●●

●

●●●●●

●

●

●●●

●●

●

●

●●●●●

●

●

●

●●●●●●

●

●

● ●● ●●●●●●

●

●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●

● ●●

●

●

●

●

●●

●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●●●

●

●●●

●●●

●●●●

● ●● ●

●●●●●●●●●●●●●●

●

●

●

●

●●●●●●●● ●●●●●●●●●●●

●●●●

●

●

●●

●●

●

●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●

●●

●●●●●●●●●● ●●●●●●●●●●●●●●●●●

●

●●●●●●●●●● ●●●●● ●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●

●

●●●●●●●

●●●● ●●●●●

●●●●●●●●●●

●

●

●●

●●

●

●

●

●●●●

●●●●● ●●●●●●●●

●●

●●●●●●●●●●●●●●

●

●●●●●

●●

●●●●●

●

●

●●

●●●●●● ● ●●

●●●●

●●●●●

●●

●●

●●

●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●●●●

●

●●●●●●●●

●●●●●●●

●

●

●

●●

●

●●●●●●●●●●●●●●●●●●●

●

●●

●●●●●

●●●●●●●●●

●

●

●●●●●

●●●●●●●●●●●● ●●● ●

●

●●●●

●●● ●●●●●●●●

●

●●●●●●

●●●●●●●●●

●

●●●●

●

●● ●●

●●

●●●●●●●●● ●●●●●

●

●●●

●●●

●●●●●●●

●●

●●●●●●●●●●●●●●

●

●●● ●

●●●●●●●●●●●●●●●●●●●●●●●● ●●●

●●

●● ●

●

●●●

●

●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●●●●●●●●●●●

●

●●●●

●

●●

●

●●

●

● ●

●●●●●●

●

●

●●●●●●●●●●●● ●●●●

●●

●●●●●●●●

●

●●●●●●●

●

●●

●

●●●●●●●●●●●●●

●

●

●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●● ●

●●

●●●

●●●●

●

●

●

●

●

●●●●●●

●

●

●

● ●● ●

● ●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●

●●

●

● ●●●●●●●●●●●●●●●●●● ●

●●

●

●

●

●●

●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●●●

●

●

●●●●●●●●●

●

●●●●●●●●●●

●

●

●●● ● ●●●

●●●

●●

●●●●●

●

●●

●●●●

●

●●●●

●●●

●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●

●●

●

●●●●●●●●●

●

●

●●

●

●●

●●●●●●●●●●●●

●●

●●●●●●●●

●

●●

●●●

●●

●

●●

●

● ●

●

●●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●

●

● ●

●

●

●

● ●●●●●●

●●●●

●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●●●●●

●●●

●

●●●

●●

●●

●

●●●

●

●●

●●

●●●

●●

●

●

●●●

●●

●●●●●●●●●●●●●●●

●

●●●

●

●●●●●●

● ●●

●●

●

●

●

●●

●

●

●

●

●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●

●

●●●●●●●●●●●●

●●

●

●●

●

●●●

●

●●●●●● ●●●●●●●●●●●

●●●● ●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●

● ●● ●●●

●●

●

●●●●

●

●●●●●●

●

●

●

●

●

●●●●●●●

●

●

● ●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●●●●

●

●●●

●

●●●●●●●

●

●

●●●●●●●●●●

●

●

●●●●

●

●

●

●●●●

●

●●● ● ●

●

●

●

●●●

●

●●●●●●●●●●●●●●●●●●●

●

●

●●●●●●

●

●●●

●

●●

●● ●●

●

●

●●

●

●

●●●

●●

●

●●● ●●

●●●

●

●●

●●●●

●

●●

●

●●

●

●

●

●

●●●●●

●●●●●●●●●●●●●●

●●

●

●

●

●

●●●

●

● ●●●●

●●●

●●●

●

●● ●●●

●

●

●●●●

●

●●●●

●

●

●●●

●

●●●●

●

●●

●●●

●●●●●●●

●

●●●●●●

●

●●●

●●

●

●●●●

●

●●

●

●●●●●

●

●

●●●●●●●●●●

●

●●●●●●

●

●

●

●●●

● ●●

●

●

●

●

●

●

●●● ●●

●

●

●

●●●●●●● ●●●●●

●●

●●●●●●●●●●●●●

●

●●

●

●●

●

●

●

●

●

●

●●●

● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●

●

●●●

●

●

●

●

●

●

●

●●●●

●

●

●

●

●●

●

● ●●●

●●●●●●●●●●●●

●

●●

●●● ●

●●

●●

●●

●

●●●●●●●●●●●●●

●

●

●

●

●

●

●

●●

● ●●●

● ●●●●

●

●●

●

●●

●

●●

●

●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●● ●● ●●●●●●● ●●

●

●●●●●●

●●●●● ●

●

●●●●●●

●

●●

●

●

●

●●

●

●● ●●●● ●●●●

●

●●

●●

●●●●●●●●●

●

●●●●●●●●

●●

●●

●

●

●●

●

●●

●● ●

●●● ●●

●

●●

●

●

●●●●

●●

●●●

●

●

●●●●●●●●●

●

●●

●

●

●

●●●

●

●●●●

●●

●

●●●

●●●

●

●●●●●

●

●

●

●

●

●●●●●●●

●●

●

●●

●

●

●

●

●●

●●●●●●●●

●

●●●

●

●●

●

● ●

●

●●

●

●●●●●

●

●●●●●●●●●●●●●

● ●●● ●●●●●●●●●●●●

●●

●●

●

●●

●

● ●●● ●

●● ●●

●● ●●●●●●●

●●

●

●

●

●●

●

●

●

●●●

●●● ●●

●●

●●

●●●

●●

●●

●

●●●●●

●

● ●●●

●

●●●

●

●●●

●

●

●●●●●

●

●

●

●●●

●

● ●

●

●

● ●

●●

●●●●●●●

●

●●●●●●

●

●●●●●

●

●●●●●

●

●●●

●●

●●●

●

●●

●●●●●●●●●●●●●●

●

●●●

●

●

●●●●●●●●●

●●●●●

●●

●

●

●

●

●

●●

●●

●●● ●●

●

●●●●●●●

●

●●●

●●

●

●●●● ●●●

●

●

●

●

●

●

●●●●●

●

●

●●

●

●

●●●

●●●●

●●

●●●●●

●●

●●●●

●

●●●●●●●●●

●●●

●

●●●●●●

●

●●● ●●

●

●

●

●●●●

●●

●●● ●

●●

●

●

●

●

●●●●

●●●●

●● ●●●

●

●●● ●●●●

●●●

●●

●

●●

●●●●●

●●

●

●●●●●●●●●●●● ●

●

●●●●●●● ●●●

●●

●

●

●●● ●●

●

●

●●

●

●●●●●

●●

●

●●

●●●

●

●

●

●

●

●

●●●●●

●

●

●

●

●●●●●●●

●

●●●

●●

●

●

●

●●●

●

●●

●

●

●●

●

●

●●●

●

●

●●●●

●●

●●● ● ●●

●●●●●

●

●

●

●●●●●●●●●●●●●●

●●

●●●

●●● ●●●●●●●●●●●●●●●●●●●●

●

●●

●●●

●●●●

●● ●

●●

●

●

●

●

●

●

●●

●

●●●

●

●●

●

●●●

●

●●●●●●

●

●●●

●

●

●

●●●●●

●

●

●●

● ●●

●●

●●

●

●●●●●●

●

●●●●●●

●

●

●

●●●

●

●●●

●

●●●●●

●●●●●●●

●

●●

●

●●

●● ●

●●●●

●●

●

●

●

●●●

●

●●●●●●●●●●●

●●

●

●●

●

●

●

●●●

●●

●

●

●●●

●●

●

● ●●

●

●●

●

●●

●

●●●

●

●●

●

●

●●●

●

●●

●●

●

●●●● ●●●●

●

●

●

●●●

●●

●●

●●●

●

●●

●●

●●●

●

●●●

●

●●●

● ●

●●

●●●●

●●●

●●

●

●

●

●●●

●●● ●

●

●●

●

●●●●●●●●

●

●●●

●●●●

●

●

●

●

●

●

●

●●

●●

●●●●●●

●●

● ●●

●●●

●

●●●

●

●

●●

●●● ●●

●●●

●

●●

● ●●

●

●●

● ●

●●●●●●●● ●

●

●●

●●●●

●

● ●●

●

●●

●●

●●

●

●

●●

●●

●

●

●●●● ●

●

●

●●●

●●

●●

●

●

●

●●●

● ●

●●

●●

●●

●

●

●●

●●

●

●●

●

●● ●●

●●

●●●●●●●●

●●

●

●●

●●

●

●

●

●

●●

●●●●

●

●

●●●●

●

●●

●

●

●

●

● ●

●

●

●●●●●●●

●

●●●

●

●

●

●

●

●

●●●●

●

●●

●●

●

●

●

●

●●

●●

●

●

●●●

● ●●

●

●●

●●

●●

●●●●●

●●

●

●●

●●

●

●●●

● ●● ●●

●

●

●●●●

●

●●

●

●

●●

●

●

●●●

●

●

● ●●

●

●● ●●●●

●

●

●●

●

●

●

●

●●●●●

●

●●

●● ●●

●●●

●

●

●

●●

●●

●●●

●●●●

●

●●●

●

●

●

●●

●

●

●●

●●

●●●

●● ●

●

●

●

●●

●

●●●●●

●

● ●

●●●●●

●

● ●

●●

●● ●

● ●●●●

●

●●

●

●●●●●●●

●

●●●

●

●

●

●●●●

●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●●●●●

●

●

●●●●●●●●●●●

●●●●●

● ●●●●●

●●

●●

●

●

●

●●

●

●

●

●

●●

●

●●●●

●

●●●●

●

●●

●

●●●●

●

●●●

●●●●● ●

●

●●

●

●●●●

●

●●

● ●

●

●●

●

●

●●●●●●●

●●●

●●

●

● ●●●●

●●●●● ●

●●●

●

●●●● ●●

●●

●●●

●

●●●●●

●

●

●●

●●

●

●

● ●● ●●

●

●

●

●

●●●●

●

●

●●●●●●●●

●

●●●

●●

●●●●●●●●

●

●●●●

●

●●

●

●

●●●● ●●●●

●

●●●●

●●

●●

●

●

●●

● ●●

● ●

●

●●

●●●

●●

●●●

●●

●

●

●

●

●●

●●

●

●●

●

●●

●

●●

●

●●

●

●●●

●●

● ●●●

●●

●●

●

●●●●●●

●● ●

●●●

● ●●

●●

●

●●

●

●

●●●

●

●●●●●●●●●●●●●●●

●●

●

●

●●● ●

●

●

●●

●●●●●●

●

●● ●●●●●●●●●

●

●●●●●●●●●

●

●●●●●

●

●

●●●

●

●●● ●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●

●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●●

●●●

●●●

●

●

●

●●●●●●●●●

●●

●

●

●●●●● ●●●

●●●● ●●●

●

●●●●●●●●●●

●

●●●●●●●

●

●●●

●

●●●

●

●●

●●●●

●●●●●●●●●●●●

●

●●●●●●●●●●●

●●●●●●●●●

●

●●●●●●●●●●●

●

●●●●●●●●●

●

●●●

●

●

●●●●●●●●●

●

● ●●●●

●

●●●●●●●● ●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●

●●●●

●

●●●●

●

●●●●●●

●

●●● ●●●●●●●

●

●●●

●

●

●

●

●●●●● ●●

●●

●●●●

●●●

●●●

●● ●

●●

●●●

●

●●●●

●

●

●●●●●●●●●●●●●●●●●●●

●

●●●

●●●

●●●●●●

●

●●●●

● ●●

●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●●●●●● ●

●●●●●

●

●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●

●●● ●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●

●

●

●

●●●●●● ●

●

●●

●●

●

●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●

●

●●●●●●●●●

●

●

●

●

●●●●●●●●●●●●●● ●●●●●

●

●●●

●

●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●● ●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●

●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●

●●●

●●

●●●

●●

●●●●

●

●●●

●

●●●●●●●●●●●●●●●

●

●● ●●●

●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●● ●●●●● ●●

●●●●●

●●

●

●

●●●●●●

●●●●●●●● ●●●●●●●●●

●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●

●

●●●

●●

●●●●●●●

●

●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●

●●

●● ●●●

●●

●

●

●●●●●●●

●●

●●●●●●●●

●

●●

●●●●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●

●●●●●●●●●●●●●●●●

●

●●● ●●

●

●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●● ●●●●●●●

●●

●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●

●●●

●

●

●●●●●●●●●●●●

●

●

●

●●●●●●● ●● ●●●

●

●●●●●●●●●●●●●●●

●●●●

●

●●●●

●

●●●●●●●●●●●●

●●

●

●●●

●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●●●●●●●●●●●●●●●● ●●

●●●●●●●●●

●●●

●

●●●●●

●

●●●●●●●●●●●

●●●

●●●

●●●●●●●●

●

●

●

●●●●

●

●●

●●●

●

●

●●●●●●●●●●●

●●●●●

●

●●●●●●●●●● ●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●●●●●●●●●● ●

●

●

●

●●●●●●●●●

●

●●●●●

●

●●

●

●●●●●●

●

●●●●

●

●●●●●●

●

●●●●●●

●●●●●●●●●

●

●●●●●●●●●●●●●●● ●●●

●●

●●●●●●

●

●●●●●●●●●●●

●●

●●●●●●●●

●

● ●●

●

●●

●●●●●●●●●●

●●

●

●

●

●● ●●●●●●●

● ●

●●●●●●●●●●●●●●●●●

●●●●●

●●

●

●

●●●

●●

●

●●●●

●

●●●●●

●

●

●

●●● ●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

● ●

●

●

●

●●●●

● ●

●●●●

●

●

●●●●

●●

●●

●

●

●●●

●

●●●●●●

●

●●

●

●●●●●●●●

●●●●●●●●● ●●●●●●●●

●

●●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●●●●●●

●

●●

●

●●●

●

●

●●

●●●

●●●●

●

●

●

●

●

●

●

●●●

●●●

●

●●●●●

●●

●●●●

●●●

●●

●

●

●

●●

●

●●●●●

●

●●●

●●

●

●

●●●

●●●●●

●●

●●●●●●●● ●●

●●●

●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●●●

●●●●

●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●

●

●

●

●●●

●●● ●

●

●

●●

●●

●

●●

●

●

●

●●

●●

●●●●●●●●●●

●●

●●●●●

●

●●●●

●

●

●●● ●

●

●

●●●●●●●●●●

●

●

●

●● ●●

●●●

●●●●

●

●

●●●

●●●

●

●●●●●●●●●●●●●●●●

●●

●●●●

●●

●

●●● ●●●

●

●●●●●●● ●●●●

●

●

●

●●●

●

●

●●●●

●

● ●●

●

●

●

●●●●

●●

●●●●●●●●●●●●●●●●●●●●●

●

●

●●●

●

●●●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●

●

●●●●●●●●●●●●●

●●

● ●● ●●●●●

●

●●●●

●

●●●●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●●●●●●●

●

●●

●

●

●●●●● ●●●●

●●

●

●●●●●●●●●

●

●●●●●●●● ●●

●●●

●

●●●●●●

●

●

●

●

●●

●

●

●●

●●●●●

●●

●

●●●●●●

●●

● ●

●●●●●

●

●

●●●●

●

●●●

●

●

●

●

●

●

●●●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●● ●●

●●●●●

●●●●●●●●●●

●

●

●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●● ●

●

● ●

●

●●●

●

●●●

●●

●●

●

●

●

●●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●●

●

●●

●●● ●●

●

●

●●

●●

●

●

●●●●●●●●●

●

●

●●●

●

●

●●●●

●●

●●

●

● ●● ●

●●

●

●●

●

●

●●

●●

●

●

●

●

●●●● ●●

●

●●●●●●

●●●●●

●●

●

●

●●●

●

●

●●●●●

●● ●

●●

●●

●

●

●

●

●●●●●● ●●●●●●●●●●●●●●●●●●●●

●

●●●●● ●● ●

●●●

●

●

●

●

●

●●

●

●

●

●

●●

●●●

●

●●●●

●

●

●●●

●

●●

●

●

●●

●

●

●●●●

●

●

●●

● ●●●● ●●●●●●●

●●●●●●●

●●

●

●●●● ●●●●●●●●●

●

●

●

●●

●●

● ●●●

● ●●●● ●●

●

●●●

●● ●

●●

●●

●●●● ●●

●

●●●●●

●

●

●

●

●

●●●●

●

●●●

●●●●●●

●

●

●

●

●

●●

●

●

●●

●●●●●●●

●●●

●●

●

●●●

●

●

●

●●

●●●●

●●

●●●●●●

●

●

●

●

●●

●

●●●●●●●

●

●

●●●●●●

●

●●

●

●

●●

●●●●●

●

●●

●● ●●●●●

●●

●

●●●●●●

●

●

●●●●●

●●

●

●●

●●

●● ●

●● ●●

●●

●●●

●●●

●

●●

● ●●

●

●●

●●●

●●

●

●●

● ●

●

●●●●●●

●

●●

●

●

●

●● ●●

●

●

●●●●

●

●●

●

●

●●

●

●

●

●●●●

●●●

●

●

●●●●

●●●

●●●●●●

●

●●●

●●●●●

●●

●●●●●

●

●●●

●

●●

●

●

● ●●●●

●●●● ●●●●●●●●●●●● ●●

●

●●

●

●

●●●

●●

●● ●●

●●

●●

● ●●

●●

●●●●

●●

●

●●

●●●

●● ●●●

●

●●● ●

●

●

●

●

●

●

●

● ●●

●●

●●

●

●

●●

●

●●●●●●

●●●●●●●●●●●

●●●● ●

●●●●●●

●●●●

●

●●●

●●

●

●● ●●●

●

●

●

●●●

●

●

●●●

●

●

●

●●●●

●

●

●

●●●●●●●

●

●

●●●●●●● ●

●

●●

●●

●

●

●●●●

●

●●●

●

●●●

●

●●●

● ●●

●

●

●

●

●●

●

●●

●●●

●

●

●

●●●

●

●

●●●●

●●●●●

●●●

● ●●

●●

●●●●●

●●

● ●●●●

● ●

●

● ●●●

●

●●●●●●●●● ●

●

●

●●

●●

●●●

●●

●●●

●

●

● ●●

●●

●●●●●●●●●●●●●●●● ●

●

●●

●●●●●●

●

●

●

● ●●●

●● ●

● ●●

●

●●● ● ●●

●

●

●

●

●

● ●●●●●●●●

●

●● ●

●●●●

●●●

●

●●

●

●●

●●

●●●●

●●●

●

●

●

●●●●●●●●

●

●●

●

●●●

●●●●●●

●

●

●●

●

●●●●●●●

●

●

●●●

●

●

●

●

● ●

●

●

●

●●●●●●●●●

●

●

●

●

●

●●●●●●●●●●

●

●●●●●● ●● ●● ●●

● ●●

●

●

●●

●

●●●

●

●●

●

●●●●

●

●

●

●

●●

●●

●

●● ●

●●

●●

●

●

●

●●●●●●

●

●

●

●●

●

●●

●

●●

●

●●

●

●

●

●

●

●

●

● ●

●●●

●

●

●

●

●●●●● ●●●●●●

●

●

●

●●●●●●●●

●

●●

●

●●●●

●

●●

●●●●●●●●●●●

●●

●●● ●

●

●●

●●

●●

●●●●

●

●●●●●

●●

●

●●

●● ●

●

●● ●

● ●●●●●

●● ●

●●● ●●●

●

●●

●

●●●●●

●● ●●●

●●●●●●●

●●●

●

●●●●●●●

●

●

●

●

●

●

●

●

●

●

●

●●●●●●●

●

●

●●

●●●●

●

●●

●●

●●

●●●●

●

●●●

●●●●●●●●

●●●

●●

●●●

●

●●●●●● ●

●

●

●●

●●

●

●

●

●●

● ●●●●

●●●●●●

●●●

●●

●

●●●

● ●●●●●●

●●

●

●● ●●

●●

●● ● ●

●

●●

●

●●

●

●● ●●●

●●●●

●●

●●●● ●● ●

●

●●●●●●●●●●●

●●

●●●

●

●●●●

●

●●●●●

●●

●●●●●● ●●

●●

●●●●●●●●

●

●

●●●●●

●

●●

●

●

●●●

●●

●●●

●

●

●●●●●

●

●●●●●

●●●

●

●●●

●●●●●

●

●

●

●●

●

●

●

●

●●●●●

●

●●

●●●

● ●

●

●

●

●●●

●●

●

●● ●●●●● ●●●●●●

●

●

●

●

●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●● ●

● ●

●●●●●●

●

●●●●●●

●

●●●●●

●

●●●●●

●●

●●●●●

●

●

●

●●

●

●●●●●

●● ●

●

●

●

●●

●●

●

●

●

●●

●

●

●

●●

●

●

●

●●●

●●

●

●

●●●

●●●

●●

●●

●

●

●

●●

●●●● ●

●

●

●●●●●

●●

●

●

●

●

●

●●●●

●●●

●

●●

●●●

●●●●

●

●

●

●●

●

●●●

●●●●●●●●●

●

●●

●

●●

●

●

●

●●●●

●

●●

●●●●

●

●●● ●●

●

●●

●

●●●●●●

●

●●● ●●●●●●●●

●●●●

●

●

● ●

●

●●●●●●●●

●

●●●

●

●

●

●

●

●

●●

●

●● ●●●● ●●

●●

●●●●●●●

●

●

●

●

●

●●●●

●

●

●●

●●●●●●

●

●●●●●●

●

●●●●●

●●●●

● ●

●●●●●●●●●●●●

●

●

●

●●●

●

●

●

●●●●

●

●●●●

●●

●

●

●●●●●

●●●●●●●

●

●

●

●

●

●● ●

●●

●

●●●●●●●

●●

●●●●●●●●

●● ●

●

●●

●

●●●●●●

●●●

●

●

●

●●

●●

●

●

●●

●

●

●

●●●●

●

●

●

●

● ●●●●

●

●●

●●●●

●●

●

●

●●

●●

●●●●●●●●●

●

●●●

●●●●●●● ●●●●●●●●

●

●

●

●

●●●●

●

●●●

●●●●●

●●●

●

●

●●●●

●

●●

●●●●

●●

●●●

●

●●●

●

●●

●●●●●●●●●

●●

●●●●●●●●●●

●●

●●

●●●

●●●●●●

●●●●●●●●●●●●●●●

●● ●●

●●

● ●●●

●

●

●●●●●●

●

●

●

●●●●●●● ●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●

●●●

●●

● ●

●

●●●●●●●●●

●

●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●

● ●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●●

●

●●●●●●●

●

●●

●●

●

● ●

●

●

●

●

●●●●●●●●● ●●●● ● ●●

●

●●●●●●●●●●●●●●●●●●●●

●

●●● ●

●

●●●●●●●●

● ●●●●●●●●●●●●●●●

●

●

●●●●●●●●●●

●

●●

●

●

●

●●●●●● ●●

●●

●

●● ●

● ●

●

●●●●●●●●●●●●●●●

●

●●

●

●

●

●●●●●● ●●●●●●●

●●●

●

●

●●

●●●

●

●●

●●●●●

●

●●●●

●● ●●●●

●

●●

●●●●●●●●●●●●●●●●●●●●

●●

●●●

●

●

●●

●

●●●●●

●

●●●●●●●●●

●

●●●●●●●●●●●●

●

●●

●

●

●

●●●●●●●●●●●

●

●●●●●●●●●●

●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●● ●●●●●

●

●●

●

●

●

●●

●

●●

●●●●●●●●●●●●●●●●●● ●●

●

● ●●●

●

●

●

●●●●

●●●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●

●

●●●●●●●●●●●●●

●

●● ●●●●●●●●

●●

●● ●

●

●●●●

●

●●●●●● ●●●●●●●

●

●

●

●

●

●●

●

●●●● ●

●●

●

●

●● ●●●

●●

●

●

●●

●

●

●●●●●

●

●●●●●●●●

●●

●

●●●●●●

●

●

●

●

●●

●

●

●

●

●●

●

●

●●●●●●●

●●●●●●● ●●●●

●●●

●●●●

●

●●●●●●●●●●● ●●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●

●

●

●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

●

●

●

●

●

●●

●

●●●●

●

●●●

●

●●

●● ●

●

●

●

●●●●●●

●

●

●●●

●

●

●

●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●

●

●●

●

●

●

●

● ●●

●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●

●

●●●●●●●●

●●

●●●●●●●●●●●●●

●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●

●●●

●●

●●●●●●●●●

●●●

●●●●●●●●

●●●●●

●●●●●●●●●

●●●●

●●●●●●●●●●

●

●●

●

●●●●●●●●

●

●●●●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●●●

●●

●●●

●

●●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●● ●●

●

●●●●

●

●●●●● ●●

●●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●●●

●

●

●

●●

●

●

●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●● ●

●●

●●●●●●●●●●●●●●●●●●●

●

●●

●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●

●●

●

●

●●●●●●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●●

●●●●

●●

●

●

●

●

●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●● ●●● ●

●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●● ●●●●●●●

●●●●●●

●

●●●●●●

●●●●

●

●●●●●●●●●

●

●● ●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●● ●●

●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●

●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●● ●●●

●●●●●

●●●●●

●

● ●●●●●●

●

●●●

●

●●●●●

●●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●

●● ●

●

●

●●●●

●

●●

●●

●

●●

●●

●●●●

●

●●●●

● ●

●●●

●

●

●●●●●●●

●●●

●

●

●

●●●●●●●●●●●●●●

● ●

●●

●

●●●●●●●●●●●●●●

●●

●

●

●●

●●

●●●●●●●●

●●●●●●●●●●●● ●●●●●●●●

●

●

●●●●●●●●●●●●●

●●

●●

●

●

●

●●●●● ●

●

●●●●●●

●

●●●●●●●●●●●●●●●

●

●●

●

●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●● ●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●

●

●

●●●●●●

●

●●●

●●

●●●●

●●

●●

●

●●●●●●● ●

●●●●●●●●●●

●

●●

●●●●●

●

●

●●●●●●●●●●●●●●

●●●

●

●●●●

●●●●●●●

●

●

●●

●●● ●

●●

●●●

●●●●●

●

●●●●●●●●

● ●

●

●

●●●●●●●●●●●●●●

●

●●●●●●●

●

●●

●

●

●

●●●

●●●●●●

●

●●

● ●●●●●

●●●●

●●●●●●●●●

●

●

●●●●

●

●

●● ●●●●●●●●●●●●

●

●

●

●●●●●●●

●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●● ●●●

●

●●●

●

●● ● ●●●

●

●

● ●

●

●

●

●

●●

●●●●

●●●

●

●●

●●●

●

●

●

●●

●●

●●

●●●●●●● ●

●● ●

●●●●●●●●●●

●●●

●

●●●

●

●●●●●●●●

●

●

● ●

●●

●●● ●

●

●●

●

●●●

●

●●

●

●●

●●

●

●●

●●

●●●●●●●●●●●●●●●●●●

●

●

●●

●●●●●

●

●

●

●

● ●●●●●●●●●

●

●●●●

●

●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●●● ●●●●●●●●●●●●●●●●●

●●●

●●●●●

●

●●●●●●● ●

●●●● ●●

●●●●●●●●●●●●●●

●

●●●●

●●

●

●

●●●●

●

●

● ●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●

●●●●●●●●●●●●●

●●●●●●●●

●

●

●

●

●●●●

●

●●●●●

●

●

●●●● ●●●● ●●●●●●●●●●

●

●●●●●●●

●

●

●

●

● ●●●●●●●●●●●●

●●●

●

●●●

●●

●

●

●●●

●

●

●●●●●●●

●

●

●●

●● ●

●

●●●

●

●

●

●

●

●

●

●●

●

●

●

●

●●●●●●●●

●●

●

●

●●●●

●

●●●

●

●●●●● ●●

●●●●●●●●●●●●●●●●●

●

●●

●●●●●●●●●●●

●

●

● ●●●●●●●●●

●

●

●●●

●

●●●●●

●● ●

●●

●

●

●

●

●

●

● ●●●●

●

●

●●●●● ●●●●●●●●●●●

●●

●●●●●●●●●●●

●●

●●●

●

●●●●●●

●

●●●●●

●●

●●●●

●

●●

● ●●●●●●●●●

●

●●●●●●●●

●

●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●●

●●●

●●

●

●●●●●●

●

●

●

●●

●

●●●●

●

●●●●●●●●●●●●●●●●

●●

●●

●●●●●●

●

●●

●●

●●

●●

●●●●

●

●●

●●●

●●●●●●●●●●●●●●●●●●

●●

●

●●

●

●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●

●●●

●●●●●●●●●●●●●●●●●

●

●

●

●●

●

●●●●●●●●●●●●

●●●

●●●●●●

●●

●

●●●● ●

●●●●●●●●●●●●● ●●●●●●●●

●●

● ●

●

●

●●●●●●●

●●●●●●●●●●●●●●●●●

●●●●

●

●●●●●●●●●

●●●●● ●● ●●●●●●●

●●

●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●

●●●●●

●●●●●●●●

●

●●

●

●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●●●●●

●

●●●●●●●●●●●●●●●

●

●

●

●●●●●●●●●●●

●

●

●●

●●●●●●●●

●●●

●

●●●●

●

● ●●●

●●

●●● ●

●●

●●●

●● ●

●

●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●

●

●●

●●●●

●

●●●●● ●●●●●●●

●

●●●●●

●

● ●●

●

●

●●●●

●

●●

●●●●●●●●●●● ●

●●●●

●

●●●●●●●●●●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●

●

●●●●●●●●●●

●

●●

●

●●●●

●

●●

●●●●●●●●●●●●●●●●●

●

●●●● ●●

●

●

●

●●

●

●

●● ●

●●

●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●● ●● ●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●● ●

●

●●

●●

●●●●●●●●●●●●●●

●

●

●●

●●

●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●

●●●●

●

●●

●

●

●

●

●●●

●

●

●

●

●●●●●

●

●●●

●●●●●●● ●●●

●

●●

●

●●●

●●

●

●● ●●●

● ●●

●●●●

●

●●

●

●

● ● ●

● ●●

●

●●

●●●●●

●

●

●

●●

●●

●●●●●

●●●

●●

● ●

●●

●

●

●●●●

●●●

●

●

●

●●

●

●●●●●●

●●

●

● ●

●

●●

●●

●●

● ●●

●●●●

●●●

●●●

●●●●

●●●●

●●●

●●●●

●

●●●●●●

●●

●

●●●●●●

●

●●●●●●●

●

●●

●

●

●● ●●

●●●●●●●●●●●

●

●

●

●●●

●

●

●

●

●

●●

●●

●●●●

●●

●

●●

●

●●●●●●●

●●

●

●

●●

●

●●

●● ●

●

●

●●

● ●●● ●

●

●●

●

●

●●●●

●

●●● ●●●

● ●

● ●●●

●●●● ●●●●●●

●

●

●

●

●●

●

●

●●●●●●●●

●

●

●

●

●●

● ●●

●●●●●●●●●●●

●

●

●

●

●

●●●●

●

●

●●●●●●

●●

●● ●

●●●●●●●

●

●

●●●●

●

●●

●

●●

●●●●

●●

●

●

●●

●

●

●

●● ●●●●●●●

●●

●●●

●

●●●

●

●

●●

●

●●●●●● ●

●●

●

●

●●●●●●

● ●● ●

●●

●● ●●

●

●

●●

●

●

●

●●●●

●●●

●

●●

●

●●

●

●●●

●●●●●●●●

●

●●

● ●

● ●●

●●● ●

●●● ●●●

●

●

●●

●●●

●●

●●●●●●

●

●●●●●

●●●●

●●●

●

● ●●

●

●●●●●●●●●

●●● ●

●●●

●●●

●

●●●●

●●●

●

●●

●

●●●●●●●●● ●

●

●●●●

●

●

●

●

●●●●●

●●●●●

●●●●●●●●

●

●●●

●

●●●●●●●●●●●●●

●

●● ●●●●●●●

●

●●●●

●

●●●●

●

●

●●●

●

●●●●●

●

●

●●●●●●●● ●

●

●

●●●

●

●●

●●●●

●

●●●●●●

● ●

●

●●

●

●●●

●

●

●

●

●●●●●●

●

●●

●

●●●●●●●

●

●●●●●●●

●

● ●●●

●

●●

● ●

●●

●

●●

●●●

●●●

●●●●

●●●●●●

●

●●

●●

●

●

●

●

●●●●●●●●●

●

●●●●

●●

●●

●

●●●●●●●●

●●●

●

●

●●

●

●●

●●●

●●●

●

●

●

●●

●●

●●●●●●●●●

●

●

●

●●

●

●

●●●

●●

●●●

●

●●●●

●

●

●

● ●

●●

●

●

●

●●

●●

●

●

●

●

●

●●●●●●● ●●●●●● ●

●●●

●

●

●

●● ●

●●

●●●

●

●●

●

●

●

●●●

●●●

●●●

● ●

●●

●●

●

●●●●●

●

●●●●● ●●● ●●

●●●●

●

●●●●

●

●

●●●●●

●

●●●

●

●●●

●

●

● ●●

●●

●

●●●●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●

●

●●

●

●

●

●●● ●

●

●

●

●

●

●

●●●●●

●

●

●●●●●●

●

●

●●●●●●●

●●

●●●●●●

●

●● ●●●●●

●

●

●

●●

●

●●

●

●●●●●●●●●●●●●●●●●●

●

●●●

●●

●●●

●

●

●

● ●●●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●● ●

●●●●●●●●●●● ●

●

●●●●●●● ●●●●●●●●●● ●●●●●●●●●●

●

●●●●●●●●●●

●

●●●●●

●●

●●●

● ●

●

●●●

●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●

●

●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●●●●

●

●

●

●

●●●

●

●●●

●●

●

●

●●●●●●●●●●

●●●

●

●●●●●

●●●

●●

●

●

●●●●●●

●

●●●●●●●

●

● ●

●●●●

●

●●●●●●●●●●● ●●●●●●●●●●●●●●

●●●

●

●●●

●

●●●●

●

●●●●●●●●●●●●●●

●●

●

●● ●

●

●●●●●●●●●●●●●●●

●●

●

●

●●●●●●

●

●●●

●●●● ●●●●

●●●●●●●●

●

●

●

● ●●●●●●●

●

●●

●

●●●●

●●

●●●●●●●

●

●

● ●●●●●●

●

●

●●●●●●●●●●●●●●●●

●

●

●

●●

●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●

●● ●

●●●●●●●

●●●●●

●

●●

●●●● ●●●●●●

●

●

●

●

●

●●●●●

●●

●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●●

●

●

●●

●

●

●●●●● ●●●●● ●●●●●●●●●●●●

●

●

●

●●●●●●

●

●●●●●

●

●

●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●●●●●●

●●●●●●●●●●●●●● ●●

●●●●●●●●●●

●

●●●●

●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●●

●

●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●● ●●●

●●●

●

●

●

●●

●●●●

●

●●●●●●●●●●●●●●●

●●

●●

●●●●●●●●●●●●●●

●

●●●

●●●●●

●●● ●●

●●

●●●●

●

● ●●●●●● ●

●

●●●●●●●●

●●

●●●●●●●●●●●●●

●

●

●

●

●●●●●●●●●●●●●●●●

●●●

●

●● ●●●●

●

●

●

●

●●●●●●●●●

●

●●●

●●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●

●

●●●●●●●●●●●●●●●

●●

●

●●●●●●● ●●●

●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●

●

●● ●●●●●●

● ●

●●

●

●●●●●● ●

● ●●●●●●●●●●●●●●●

●●

●●●●●

●●●

●●●

●

●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●

●

●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●

●

●●

●

●

●●

●●●●●●

●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●●

●

●●

●●●● ●●●●●●●●●●

●

●●●●●●

●●●●●

●

●●●●●●●●●●●●●

●

●●●●

●

●●

●

●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●

●●●

●●●● ●●●

●

●●●●●●●●●●●●●●●

●

●●●●●●●●●●●

●

●

●●●●●●●●●●●●

●

●●●

●●●

●●● ●

●●●●

●●●●●●●

●●●●●●●●●●●●●●●●●●●●

●

●

●

●

●●●●●●●●●●●●●●●●●●●●●

●●●●●●

●●●●●●●●●●●●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●

●

●●●●●●

●

●

●

●●●●●●●●●●● ●●●

●●●●●●●●●●●●●●●

●

●●●●●●●●●● ●●● ●●●●●●

●

●●●●●●● ●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●

● ●

●

●

●●●●●●●

●

●

●

●●

●

●●●●●●●

● ●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●

●●●●●

●●●

●

●●

●●●●

●

●●●●●●●●●●

●

●●●●

●●

●

●

●

●

●●

●●●●●●●●

●

●

●●●●●●●●● ●●●●●●●●●●●●●●●●

●●

●

●●●

●●

●

●●●

●●●●

●●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●

●●

● ●●●●●●●

●●●●●●●●●●●●●●●●●●●●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

● ●●● ●●●

●

●

●●●

●

●

●●● ●●●●●●●●●●

●

●

●

●●●●●●●●

●

●

●●

●

●

●

● ●

●

●●●●

●●●●●●●

●●●●●●●●●●●●●●●●●●●●●●●●●● ●

●●

●●●

●●

●

●

●

●●●●●●

●

●

● ●

●

●●●●●●●

●

●●●●●● ●●●

●

●

●●

●●●

●

●

●

●

●

●●

●●●●●●●●●

●

●●

●●●

●●

●●

●●●●●● ● ●●● ●

●●

●●●

●●●●●

●

●

●

●●

●

●

●

●

●

● ●●●●●

●

●

●

●●●

●

●●

●●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●●●●●●●

●

●●●●●●●●●●●●●●●●

●

●●●●

●

●●●●●●●●●●●●●●

●

●●●●●●●●●

●

●●●●●●●●●●●●●●●●●●●●●●●● ●

●●

●

●

● ●●

●

●●

●●●●

●●

●●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●

●●●●●●

●

●●

●

●

●●●

● ●

●●

●

●●●●●

●●

●

●

●●●●

● ●

●

● ●

●●●●●●●●●●●●●●●●●●●●

●

● ●●

●

●●

●●

●●●●●

●

●●●●●●●

●●●●●●●●

●

●●

●●●

●

●

●●

●●●

●

●

● ●

●●

●

●●●●●●●●●●●●●●●●●●●●●●●●●●

●●●●●●●● ●

●

●●●●

●●● ●●●●●●●

●

●

●●●●●●

●

●●●●●●●●●●

●

●●●●●●●●●●●●●●●●●

●

●●●●●

●●●

●●

●●●

●●

● ●●

●

●

●

●

●

●●●●●●

●

●

●●

●

●

● ●●●

●

●●

●●●●●●●●●● ●●●●

●

●●●●●●●●●●●●●●●●

●

●●●●

●

●●●●●

●

●

●

●●●

●● ●

●●●●●●●●●●●

●●

●

●

●

●

●

●●

●●●●

●

●

●

●

● ●

●

●●

●

●

●●●

●

●

●

●●●●

●●●

●●●●●

●

●

●

●

●

● ●●

●●

●

●●

●

●●

●●●

●

●

●●●

●

●●●

●●●

●

●●●

●● ●●●●●

● ●●●

●

●

●

●●●●●●

●

●●

● ●

●

●●

●●● ●

●

●●●

●●●●●

●

●●

●

●●

●●●

●

●●

●●

●●●●●

●●●●

●

●

●●

●

●

●

●●

●●●

●●●●●●●● ●●

●

●●●

●●

●●●●●

●●●●●●●●

●

●

●●

●

●

●

●●●●●

●

●●●

●

●

●●●●●

●

●●

●

●

●

●

●●

●

●

●

● ●

●

●●

●

●●●

●

●

●

●

●

●●●●●●●●●●●●

●●●●●●●●●●

●●●● ●

●

●

●

●●●●

●

●●●●●●●●●●●

●

●

●●

●●●●●●

●

●●●

●●

●

●

●●●●●●●●●●●●●●●

●

●●●●●●

●

●●●●●●●●●

●●

●●●●●●

●●●●

●●●●

●●

●

●●●

● ●

●●

●

●●●

●● ●●●● ●

●●●●●●●●●●●●●●●●●●●●

●

●●●●●●●

●

●●●

●●●●

●

●●

●●●●●●●●●●●●●●●●

●

●

●

●●●●●●●●

●

●●

●●●●●

●

●●● ●●●●●●●●●● ●●

●

●

●●●●●●●●●

●

●●●

●

●● ●●●●●●

●

●●●

●●●

●

●

●

●

●

●

●●●

●

●

●●●●

●

●●●●

●●●●

●

●●●●●

●●●

●●●●

●

●●●

●●●●

●●

●

●●●●●●●●●●●●●

●●

●

●

●

● ●●

●●●● ●

●

●●

●

●

●

●●

●

●

●

● ●

●●●●

●

●●●●

●

●●

●●●●●●●●●●●●●●●●●

●● ●●●

●

●● ●●

●●●●●●●●●●●

●●●●

●

●

●

●●●●

●

●●

● ●●●

●●

●

●●

●●●●

●●●●●●●● ●●●

●

●●●●●●●●

●

●

●

●

●●●●

●

●●●●●●●●●●●●

●

●●●●●

●●

●

●●

●

●

●●●

●

●●●

●●

●●

●●● ●

●

●

●●

●●●

●

●●

●

●●● ●●●

●

●●●●

●● ●

●●●●

●

●●●●●

●

●●●●

●

●

● ●●●● ●●●

●

●●

●●●

●●●● ●●

●●

●●

●●● ●

●

●●●

●●

●●

●

●●

●●●●

●●

●●●

●

●

●●●●

●●

● ●●

0 5 10 15

−10

−50

510

A = (data.log$L192_RPKM + data.log$L193_RPKM)/2

M =

dat

a.lo

g$L1

92_R

PKM

− d

ata.

log$

L193

_RPK

M

今後の展開 RNA-Seqの低価格化、大規模化、多検体化が進むと思われる。 臨床検体など。 いわゆる、二次解析までは、ルーチン化されビッグデータ処理 技術の普及と相まって、リアルタイムでできるようになると 思われる。 データ解析的にはアセンブリやマッピングのあとの三次解析、 統計解析や視覚化、結果のアノテーションなどがデータ解析の 主流になると思われる。 データ解析環境としてのクラウドの利用の促進が図られる。

一次解析: 画像データより 配列データの取得

CASAVA

二次解析: アセンブリ

Velvet, ABYSS, SOAPdenovo,

Newbler, Trinity, Oases etc.

マッピング

Bowtie, BWA etc.

三次解析: 統計解析

R, Bioconductor, Matlab etc.

視覚化

IGV, Tablet etc.

多型解析 Samtools, GATK

etc.

ChIP-Seq MACS, QuEST

Conventional Genomic Analysis System on Linux Server

一次解析: 画像データから 配列データの取

得 CASAVA

二次解析:

Assembly: Contrail

CloudBrush,

Mapping: CrossBow,

Myrna

三次解析:

統計解析: R/Bioconductor

多型解析:GATK

系統樹:MrsRF

相同性解析：BLAST

Linux

Java Virtual Memory

HDFS MapReduce Hadoop

OS

Virtual Machine

Cloud based Genomic Data Analysis System

アマゾンウェブサ＝ビス（公共担当　吉荒様）よりの提供資料

アマゾンウェブサービス（公共担当　吉荒様）よりの提供資料

アマゾンウェブサービス（公共担当　吉荒様）よりの提供資料

非定形データ

定形データ

数理モデル化

説明変数の選択

モデル最適化

モデル検証

ビッグデータ解析のワークフロー例 Hadoop MapReduceを用いた並列分散処理、 シェルスクリプトを用いた分散テキスト処理、 NoSQL によるデータ処理、モンテカルロ法による無作為抽出

RDMS（MySQL、PostgreSQLなど）によるデータ処理、集計

統計学的有意差検定（ステューデントのt検定、マン ホイットニーのU検定）、スパースモデリング

疾患患者の識別 判別分析、サポートベクトルマシン、機械学習、ベイズフィルタリング

線形モデル、ロジスティック回帰モデル、両者の混合モデル など

ウィルクスラムダ、AIC（赤池情報量規準）、BIC（ベイズ情報量基準）など

クロスバリデーション（リーブワンアウト法を含む）

共同研究者など

l  東京農工大学文部省連携「農学系ゲノム科学領域における 　　実践的先端研究人材育成プログラム」参加者および支援者 l  徳島大学大学院ヘルスバイオサイエンス研究部 　　精神医学部門　大森哲郎、沼田周助、木下誠、伊賀淳一、 　　渡部真也 l  高知大学医学部精神神経科学講座　森信　繁 l  静岡大学 グリーン科学技術研究所　富田因則 l  ユニバーサルシェルプログラミング研究所　當仲寛哲 l  アマゾンウェブサービス l  ヒューレットパッカード l  理研SCLS l  BGI　他

支援いただいている助成など

東京農工大学文部科学省 連携事業「農学系ゲノム科学 領域における実践的先端研究人材育成プログラム」 理化学研究所HPCI戦略プログラム分野１「予測する生命科学・ 医療 および創薬基盤」スーパーコンピュータ「京」と互換性を有するSCLS 計算機システム AWS in Education Research Grant Award EDU_Research_Ishii_TokyoUAT-11-2013 有限会社ユニバーサル・シェル・プログラミング研究所　共同研究： 「シェルスクリプトによる生物化学用のデータ解析手法の開発および 生物化学用のデータ解析システムの構築」 科研費基盤研究（C）「新規ビッグデータ解析手法による精神神経系疾患 診断法の確立」

謝謝

BGI 張紅燕様 李爽様️

檀永凱様 他

講習会など

東京農工大学農学系ゲノム科学人材育成プログラムでは、特にデータ解析、 データマイニングに関連するセミナーを実施しており、学外の方にも受講できるように しています。 ご興味が有る方は、適宜ご参照、ご参加ください。 以下に詳細をお示ししています。 　　　　　　　　　　　　　http://genome.lab.tuat.ac.jp/~genome/ 2014年06月21日 東京農工大学 平成26年度公開講座「ビッグデータ•データ分析講座」 2014年07月15日 情報機構セミナー「統計学入門」、「ビッグデータ活用」 2014年12月2日　日本テクノセンター 　　　　　　　　　　　　　　　「データマイニングの基礎とビッグ・データにおける活用例」

Reminding Waterloo Bridge (1940) � Powered by

FreeBSD �