伯森蛋白質研究產品專刊伯森蛋白質研究產品專刊伯森蛋白質研究產品專刊Protein�Research�Product�Catalog�Protein�Research�Product�Catalog�Protein�Research�Product�Catalog�
Expression
LEXSY ProteinExpression System
P. 2
Purification
Affinity
Size Exclusion
Ion Exchange
FPLC Accessories
P. 3
P. 5
P. 6
P. 6
Crystallization
Screening Kit
Crystallography Starter Kit
Detergents
P. 7
P. 7
P. 7
Labeling
FluoroSpin Protein Labeling and Purification Kit
P. 8
BB伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.
網址:www.blossombio.com 客服專線:台北0800-059668��台南0800-259988��花蓮�0800-056668
台北�(02)�2788-2121 新⽵�(037)�581-106 台中�(04)�2323-3939 台南�(06)�235-6628 ⾼雄�(07)�555-0764
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Expression
2
Cultured Leishmania tarentolae cells expressing green uorescent protein.
JB-EGE-1300
JB-EGE-1410
JB-EGE-1420
JB-EGE-2002-15
LEXSYcon2 Expression Kit, for constitutive protein expression
LEXSinduce3 Expression Kit, for inducible expression from integrative constructs
LEXSinduce4 Expression Kit, for inducible expression from episomal constructs
LEXSY in vitro Translation Kit, for plasmid based cell-free protein synthesis
Cat. No. Product Name
LEXSY Protein Expression SystemLEXSY 是德商 Jena Bioscience 的獨家
蛋白質表現系統,以單細胞原蟲為宿
主,兼具了真核生物系統以及大腸桿菌
系統的優點,是您大量表現蛋白的最佳
選擇!
Bacteria Yeaste.g. Pichia
Insect cellse.g. Sf9/21
LEXSY Mammaliancells
LEXSY performs mammalian type of glycosylation (Breitling et al. 2002)
LEXSY strains grow higher densitiesthan insect and mammalian cells
(Fritsche et al. 2007)
LEXSY grows fasterthan insect and mammalian cells
LEXSY�完美克服其他蛋⽩質表現系統常⾒問題
YeastBacteriaInsect &Mammalian cells
§�⽣產蛋⽩質無後修飾§�無法摺疊成正確的構形§�具包涵體
§�後修飾與哺乳⽣物差異⼤§�細胞不易破壞,�抽取蛋⽩質困難
§�表現量低§�⽣⾧速率緩慢§�培養液價格昂貴
LEXSY�特點:
þ��不具包涵體�(inclusion�body)þ��後修飾�(post-translational�modification)�近似哺乳⽣物細胞þ��操作流程條件穩定þ��表現量⾼,產量⼤þ��不具⽣物毒性�(Biosafety�level�1)
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Purification
3
FPLC 純化膠體與管柱伯森公司擁有完整的 FPLC 純化商品,包含親和性層析、膠體過濾、
離子交換層析等,提供來自德國、瑞典與日本的優良產品。各式產品
均擁有極高性價比,歡迎進一步索取相關資料及免費試用服務!
Affinity 親和性層析His-tag 重組蛋白純化
His-tag 重組蛋白偵測
膠體
重⼒流
磁珠
預注管柱
ABT-6BCL-NTANi-10
ABT-6BCL-NTANi-100
ABT-6BCL-NTANi-500
CU-31103
CU-31105
ABT-6BCL-QHNi-C8
ABT-6BCL-QHNi-C5
MF-HIS-3000-2ML
MF-HIS-3000-5ML
BW-45-655-103
BW-45-655-104
BW-45-655-107
BW-45-655-108
ABT-AF6NI-CTG5-2
ABT-AF6NI-CTG5-5
NICKEL NTA Agarose Resin, 10 ml
NICKEL NTA Agarose Resin, 100 ml
NICKEL NTA Agarose Resin, 500ml
PureCube Ni-NTA Agarose, 10 ml
PureCube Ni-NTA Agarose, 50 ml
His-COLUMN HIGH Density NICKEL (8x1 ml)
HisXL-COLUMN HIGH Density NICKEL (5x5 ml)
Qbeads-NTA-Ni (2x1 ml)
Qbeads-NTA-Ni (5x1 ml)
BabyBio Ni-NTA (5x1 ml)
BabyBio Ni-NTA (10x1 ml)
BabyBio Ni-NTA (5x5 ml)
BabyBio Ni-NTA (10x5 ml)
NICKEL Afnity Cartridges 5ml (2x5 ml)
NICKEL Afnity Cartridges 5ml (5x5 ml)
NI Sepharose 6FF
NI Sepharose 6FF
NI Sepharose 6FF
NI Sepharose HP
NI Sepharose HP
His Mag Sepharose
His Mag Sepharose
Histrap HP
Histrap HP
Histrap HP
Histrap HP
Histrap FF
Histrap FF
Type Cat. No. Product Name GE 對應商品
JB-PS110 His Detection Kit
Detection of poly-histidine tagged proteins, ready-to-use solutionUp to 3 x 3 reactions
Cat. No. Product Name
His Detection Kit
l 快速偵測 His Tag 重組蛋白純化結果
l 不需 Blocking, Washing 等步驟
l 偵測極限可達 0.1 pmole
l 肉眼即可觀察,也可再使用儀器定量
1 hrTransfer
1-15 hrBlock
30'Wash
1-2 hr1st Ab
30'Wash
1-2 hr2nd Ab
30'Wash
5'Develop
1 hrFix Gel
TOTAL
6-21 hr
20'Wash
1 hrStain
20'Wash
TOTAL
3 hr
1 hrTransfer
30'-90'Incubation
In-GelStain
HisDetection Kit
WesternBlot
>5X�Faster�Than�Western�Blot!TOTAL
1.5-2.5 hr
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Purification
4
GST-tag 重組蛋白純化
抗體純化
其它親和性層析純化
膠體
磁珠
預注管柱
Tag�切除
ABT-4B-GLU-10
ABT-4B-GLU-100
CU-32103
CU-32105
CU-32205
CU-32303
CU-32305
CU-32307
BML-SE363-1000
Glutathione Agarose Resin, 10 ml
Glutathione Agarose Resin, 100ml
PureCube Glutathione Agarose, 10 ml
PureCube Glutathione Agarose, 50 ml
PureCube Glutathione MagBeads (5 ml)
PureCube Glutathione Cartridge (5x1 ml)
PureCube Glutathione Cartridge (1x5 ml)
PureCube Glutathione Cartridge (5x5 ml)
Thrombin
Type Cat. No. Product Name
Glutathione Sepharose 4B
Glutathione Sepharose 4B
Glutathione Sepharose HP
Glutathione Sepharose HP
GSTrap HP
GSTrap HP
GSTrap HP
GE 對應商品
膠體
磁珠
預注管柱
BB-02-01PA-01
BB-02-01PG-01
ABT-4BCLPL-R5
MF-PRA-3000-2ML
MF-PRA-3000-5ML
MF-PRA-3000-10ML
MF-PRG-3000-2ML
MF-PRG-3000-5ML
MF-PRG-3000-10ML
BW-45-605-102
BW-45-605-103
BW-45-605-106
BW-45-605-107
Protein A agarose, 10 ml
Protein G agarose, 10 ml
Protein L agarose, 5ml
Qbeads-Protein A, 2ml
Qbeads-Protein A, 5ml
Qbeads-Protein A, 10ml
Qbeads-Protein G, 2ml
Qbeads-Protein G, 5ml
Qbeads-Protein G, 10ml
BabyBio proteinA (5x1 ml)
BabyBio proteinA (10x1 ml)
BabyBio proteinA (5x5 ml)
BabyBio proteinA (10x5 ml)
Type Cat. No. Product Name
nProtein A Sepharose
Protein G Sepharose
Protein L sepharose
Protein A Mag Sepharose Xtra 5
Protein A Mag Sepharose Xtra 5
Protein A Mag Sepharose Xtra 5
Protein G Mag Sepharose Xtra 5
Protein G Mag Sepharose Xtra 5
Protein G Mag Sepharose Xtra 5
Hitrap protein A HP
Hitrap protein A HP
Hitrap protein A HP
Hitrap protein A HP
GE 對應商品
膠體
磁珠
ABT-6RRF-STV-5
CHI-676943324
CU-50303
CU-33102
MF-STA-3000-5ML
MF-STA-3000-10ML
MF-DEX-3000-5ML
MF-COO-3000-5ML
MF-NHH-3000-5ML
Streptavidin Agarose, 5ml
Cellune sulfate
PureCube NHS Activated Agarose, 10ml
PureCube Rho1D4 Agarose, 5ml
Qbeads-Streptavidin
Qbeads-Streptavidin
Qbeads-Hydroxyl
Qbeads-Carboxyl
Qbeads-Amine
Type Cat. No. Product Name
Streptavidin Sepharose
Heparin Sepharose
NHS Sepharose
Streptavidin Mag Sepharose
Streptavidin Mag Sepharose
GE 對應商品
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Purification
5
Size Exclusion 膠體過濾去鹽與置換緩衝液
Gel Filtration 膠體過濾
離⼼
膠體
重⼒流
CP-0201-Z025.0-001
CP-0201-Z100.0-001
CP-0205-Z025.0-001
CP-0205-Z100.0-001
CP-0202-Z025.0-001
CP-0202-Z100.0-001
TM-0102-E100.0-001
TM-0102-E500.0-001
TM-0105-E100.0-001
TM-0105-E500.0-001
TM-0106-E100.0-001
CP-0109-Z050.0-001
CP-0104-Z050.0-001
CP-0105-Z025.0-001
Z-50 MINI Spin Columns, 25pk
Z-50 MINI Spin Columns, 100pk
Z-25 MINI Spin Columns, 25pk
Z-25 MINI Spin Columns, 100pk
SEQ Z-50 MINI Spin Columns, 25pk
SEQ Z-50 MINI Spin Columns, 100pk
Zetadex-25 Fine, 100g
Zetadex-25 Fine, 500g
Zetadex-50 Fine, 100g
Zetadex-50 Fine, 500g
Zetadex-50, 100g
N2 Gravity Columns (1-200 µl), 50pk
N10 Gravity Columns (0.5-1 ml), 50pk
N25 Gravity Columns (1-2.5 ml), 25pk
Type Cat. No. Product Name
Microspin G-50 columns
Microspin G-50 columns
SpinTrap G-25
SpinTrap G-25
AUTOSEQ G-50
AUTOSEQ G-50
Sephadex G-25 ne
Sephadex G-25 ne
Sephadex G-50 ne
Sephadex G-50 ne
Sephadex G-50 DNA Grade
MiniTrap G-25
PD-10 Desalting Columns
PD-10 Desalting Columns
GE 對應商品
膠體 EMP-TM-0501-E100.0-001
EMP-TM-0501-E500.0-001
ABT-A-1020S-1000
ABT-A-1040S-1000
ABT-A-1060S-1000
ABT-A-1021S-1000
ABT-A-1041S-1000
ABT-A-1061S-1000
Zetadex 20-LH, 100g
Zetadex 20-LH, 500g
2% B Agarose Bead
4% B Agarose Bead
6% B Agarose Bead
2% BCL Agarose Bead
4% BCL Agarose Bead
6% BCL Agarose Bead
Type Cat. No. Product Name
Sephadex LH-20
Sephadex LH-20
Sepharose 2B
Sepharose 4B
Sepharose 6B
Sepharose CL-2B
Sepharose CL-4B
Sepharose CL-6B
GE 對應商品
BabyBio Ni-NTA Mini Columns Z-25 and Z-50 MINI Spin Columns N2, N10, and N25 Gravity Columns
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Purification
6
Ion Exchange 離子交換
膠體
預注管柱
CHI-675982327
CHI-2120051
CHI-675980327
CHI-675983327
CHI-1990751
CHI-2120051
CHI-1980551
CHI-1980051
BW-45-100-107
BW-45-200-103
BW-45-200-107
BW-45-150-103
BW-45-150-107
Cellune Q, 100ml
Cellune S, 100ml
Cellune A, 100ml
Cellune C, 100ml
Cellune Q (5x1 ml)
Cellune S (5x1 ml)
Cellune A (5x1 ml)
Cellune C (5x1 ml)
BabyBio Q (5x5 ml)
BabyBio S (5x1 ml)
BabyBio S (5x5 ml)
BabyBio DEAE (5x1 ml)
BabyBio DEAE (5x5 ml)
Type Cat. No. Product Name
Q Sepharose
SP Sepharose
DEAE Sepharose
CM Sepharose
Hitrap Q FF
Hitrap SP FF
Hitrap DEAE FF
Hitrap CM FF
Hitrap Q HP
Hitrap S HP
Hitrap S HP
Hitrap DEAE HP
Hitrap DEAE HP
GE 對應商品
FPLC Accessories 純化配件
空管柱
磁座
C-50
CXL-50
MC-25
MC-100
ABT-FPLC8-3
ABT-FPLC30-2
ABT-FPLC45-2
ABT-FPLC80-1
O-MAGRACK
PLASTIC columns, 12ml
PLASTIC XL columns, 35ml
PLASTIC MINI columns, 25pk
PLASTIC MINI columns, 100pk
EMPTY FPLC 8ml Columns, 3pk
EMPTY FPLC 30ml Columns, 2pk
EMPTY FPLC 45ml Columns, 2pk
EMPTY FPLC 80ml Columns, 1pk
Mag Rack (1.5 ml x 8 well)
Type Cat. No. Product Name
COLUMN PD-10,EMPTY
Microspin columns
Microspin columns
GE 對應商品
Cellune Chromatography Media PLASTIC Columns (12ml & 35ml) Mag Rack
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Crystallization
7
Screening Kit蛋白質結晶條件多如繁星,JBScreen 系列商品幫助實驗者快速篩選出最適宜的結晶條件!
提供 240 種最常見的緩衝液與沉澱劑之組合
整理出學者研究最容易成功的 96 種條件, 廣泛的 pH 範圍、多種沉澱劑
由 JCSG 機構發表,以稀疏矩陣為原理的篩選組合
針對 Protein Kinase 的初期結晶所設計,涵蓋了 96 種常見結晶條件
對於 PEG 3350 與 PEG 5000 MME 與 48 種鹽類組合的條件篩選
JBScreen Classic
JBScreen PEG/Salt
JBScreen Basic
JBScreen JCSG++
JBScreen Kinase
請前往下列網址瀏覽完整產品項目,或使用 QR code 以獲得進一步資訊
www.jenabioscience.com/cms/en/1/browse/632_crystal_screens.html
™Crystallography Starter Kit
Detergents
l 包含一系列實用工具,讓使用者以省時省力的方式獲取結晶
l MicroMounts™ 具獨家光圈設計,減少取結晶時多餘液體
l 相容於常見 X 光設備,並具極低 X 光背景值
l 堅固耐用,即使在低溫條件下也不變形
JB-CSK-2 Crystallography Starter Kit, with everything to mount and collect X-ray data from your crystals
Cat. No. Product Name
Each kit contains 20 Dual-Thickness MicroMounts™ (M2-L18SP-A2), with 5 each of 75, 100, 150 and 200 µm apertures.
CU-16003
CU-16007
CU-16011
CU-16015
CU-16019
CU-16043
CU-16023
CU-16031
CU-16039
CU-16092
CU-16093
Octylglucopyranoside (OG) (5x5 g)
N,N-Dimethyl-n-dodecylamine N-oxide (LDAO) (5x5 g)
n-Decyl-beta-maltoside (DM) (5x5 g)
n-Dodecyl-beta-maltoside (DDM) (5x5 g)
n-Octyl-1-thio-beta-D-glucopyranoside (OTG) (5x5 g)
n-Decylphosphocholine (Fos-Choline™ 10) (5x5 g)
n-Dodecyl-phosphocholine (Fos-Choline™ 12) (5x5 g)
n-Tetradecylphosphocholine (Fos-Choline™ 14) (5x5 g)
n-Hexadecylphosphocholine (Fos-Choline™ 16) (5x5 g)
Detergent Screening Set Classic (1 g each of OG, LDAO, DM, DDM, OTG)
Detergent Screening Set Phosphocholines 1 (1 g each of Fos-12, Fos-14, and Fos-16)
Features Cat. No. Product Name
純度 >99.5%
專供結晶學使用
for�Sample�Retrieval
伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121
Labeling
8
FluoroSpin Protein Labeling and Purification Kit
FluoroSpin 能將廣泛的螢光物質與大於 25 kDa
的抗體、酵素與其它蛋白質快速地接合與純化。
其特點如下:
l 快速 – 標定加上純化,10分鐘就完成
l 簡單 – 只要三步驟:Mix, Incubate, Purify
l 精確 – 可精確控制蛋白質標定的程度
l 方便 – 產品內已包含 Spin Columns
市⾯上最快速的�Protein�Labeling�Kit�!�
10�分鐘內完成標定�!
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