草地研究室
指導老師:王裕文 老師報告學生:楊雯惠報告時間: 3月 21 日上午 10 點 35 分報告地點:農藝館 112
作者: Yiping Zhang and John R. Stommel
出處: Crop Science 41 (2001) 1602–1608
草地研究室
β - carotene
Phenotypic selection
Marker-assisted selection
• Provitamin A carotenoid
• Essential nutrient
• Antioxidant
• Reduced risk of heart disease and certain cancers
Beta gene
草地研究室
Marker-assisted selectionRAPD
• Simple
• Rapid
• Inexpensive
• Radioactive isotopes
• Analyzing large numbers of samples
草地研究室
Marker-assisted selectionRAPD
• Poor reproducibility
• Multiple amplification products
• Dominant marker
草地研究室
How to solve ?
Modification of PCR parameter
Restriction enzyme digestion
Primer redesign
• increasing annealing temperature
• increasing Mg++ concentrations
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