Doxorubicin (3h): - + - + - +- +WT A7 D7

Doxorubicin:

Flag-FOXO3a

FOXM1

p27Kip1

Tubulin

Flag-FOXO3a

MCF-7

MCF-7

P-FOXO3a (Ser7)

Flag-FOXO3a

p27Kip1

Cyclin E

Bim XL

Tubulin

0 3 9 24 0 3 9 24 0 3 9 24

D7A7WT

Cleaved PALP

(h)

Flag-FOXO3a