Factors affecting on semen quality
ผศ.น.สพ. ชยัวฒัน์ จรสัแสงภาควชิาศัลยศาสตรแ์ละ
วทิยาการสบืพนัธ ุ์
•ใหนั้กศึกษาอธบิายปัจจยัท่ีทำาใหเ้ชื้ออสจุิตายมา 1 ตัวอยา่ง
References• Barth A.D and Waldner C.L. 2002. Factors
affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine. Can Vet J 43(3):274-284
• Hafez.E.S.E and Hafez.B. 2000. Reproduction in farm animals 7 th edition. Lippincott Williams & Wilkins. 509pp
• http://vet.kku.ac.th/chaiwat/teaching/teaching2010
Objectives• บอกสาเหตท่ีุมผีลกระทบต่อเชื้ออสจุไิด้
• สามารถอธบิายปัจจยัจากตัวสตัวต่์อคณุนำ้าภาพของเชื้อ
• สามารถชีใ้หเ้หน็ถึงอิทธพิลของสิง่แวดล้อมท่ีมผีลต่อคณุภาพนำ้าเชื้อ
• สามารถบอกถึงปัจจยัท่ีอาจมผีลต่อคณุภาพนำ้าเชื้อในขัน้ตอนการผสมเทียม
Breeding soundness evaluation
Pedigree
Progeny test
- Long time - More costly
Breeding soundness evaluation
Breeding Index
(USA TPI=Type production index) (Canada LPI= Lifetime profit index)
(New Zealand BV’S = Breeding values) (Japan NTP=Nippon total index)
Terminology of semen
•PTA (ETA) : Predicted or estimated transmitting ability
•EBV : Estimated breeding value (Canada)
•PTAT : Predicted transmitting ability of type traits
•Reliability
Factors on Semen quality
•Bulls effect•Feeding •Diseases•Semen collection management
•Environmental effects
Bulls affecting • Age• Scrotal circumference• Breeds• Body condition score• Physical abnormalities• Frostbite ???
Effect of Age
Effect of Breeds
Effect of BCS
Effect of Physical abnormalities
Effect of Scrotal circumference
Minimum Recommended Scrotal Circumference in Centimeters by Age for Bulls
Age (months) Scrotal Circumference (cm) 15 30>15-18 31>18-21 32>21-24 33>24 34
Effect of scrotal frostbite
- Puberty --> scrotal circumference
- semen
collection time- 6Bull > years- Boar > 3
years- Stallion > 20
years
Feeding on semen quality
Feeding
R RRRRRRR R RRRRRRR( )
Young animals- -- Low energy > delay semen production- --R> less affecting- High energy ---> low libido
Thailand
-- > Late puberty
- roughage- climates
Low feeding
- Testosterone --> decrease- low libido- Obesity -->
low libido and sexual activity
Vitamin A
Pituitary gonadotropins
Testicular degeneration
Vitamin E ---> Male organ
Vitamins
Minerals
- -- Iodine > poor libido- Copper- Cobalt- Zinc- Manganese
Toxins - -- High gossipol > epididymal cell
- radiation --> spermatogenesis
- --- estrogen feed > failure of sperm transport in the female tract
Diseases
- Virus (IBR, BVD, FMD)
- Bacteria (Brucellosis)
- Parasite (Blood parasites)
Semen collection management
- Training- - Frequency 1 2 per week- hoof problem
--> electro-ejaculator
Environmental effects 1. Temperature- Motility - Temperature shock
- Heat shock- Cold shock
2 . Photoperiod & seasonLong time --> Longevity sperm
3. Oxygen ionO 2 --- > H2O2
4. AciditypH 6.7- 6.9Sperm metabolism --> Lactic acids
---> Buffers5. Osmostic pressure
Permeabili ity 6. Metal
Lead, Mercury 7 . Bacteria 8 . Detergent&Toxin
1. Semen collection
2. Storage and freezing
3 . Storage and transportation
4 . Thawing and insemination
Risk of environment factors
Bulls prepare- Shower- Wash prepuce with NSS 0.9 %
Material prepare- Detergent- Sterile
Collection time- Light- Temperature
Semen collection
Storage and freezing
1. Freezing 37 C 5 C - 20 C - 79 C -196 C
2. Extender
- protection cold shock and cryo-protectant- Energy source- Buffering- Osmotic pressure- Bacteria inhibition
- Extender Egg Yolk Tris
- --- Tris > buffers- -- citric acid > buffers- -- Glucose > energy, osmotic pressure- Penicillin, Streptomycin, Polymixin B- Gl -- - ycerol > cryo protectant- - -- -Egg yolk > cyro protectant
Storage and Transport
Semen bank tank- coding- liquid nitrogen
AI center tank- liquid nitrogen--> 2 weeks
Inseminator tank- liquid nitrogen --> day by day
Transports- Liquid nitrogen- Tank to tank
- Canister - Straw (0.25 French mini-straw
0.5 ml USA)- < 15 second- forceps
Thawing and Insemination
Thawing- canister level- forceps- 35-40 C 30 second
AI- AI gun --> clean & dry
AI technique
- - Sperm death 10 20 %
- Live sperm 6- 8 m. / straw
-Progressive motile drop - 515%
-Limited of bulls
-Health control
•จะเกิดอะไรขึน้ถ้ามนี้ำ้าปนเปื้ อนในขัน้ตอนการรดีน้ำ้าเชื้อ ???
Recommended
