Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its
Application to Glucose Detection
By Yujun Song, Konggang Qu, Chao Zhao, Jinsong Ren, and Xiaogang Qu*
Introduction
1.GN的研究现状使其在医药诊断和生物技术方面的应用成为可能;
2.本文提出 GO-COOH有类似过氧化物酶的作用,并简介研究成果。
1.提出过氧化物酶作为H2O2和葡萄糖诊断试剂的应用还有广阔的发展空间;
2.简介葡萄糖传感器的研究现状(特别强调了下HRP),并将本文中的GO-COOH 和 HRP进行了简单的对比。
Simple
Cheap
highly sensitive
highly selective
Catalytic mechanism
Experimental
Expandable graphite flake + NaCl
ground 10min
H2O
filtration
Hummer’s method
GO-COOH [1]
[1] X. M. Sun, Z. Liu, K. Welsher, J. T. Robinson, A. Goodwin, S. Zaric, H. Dai,Nano Res. 2008, 1, 203.
AFM
FTIR
EDX
H2O2+GO+TMB
Glucose+GOx+GO+TMB
UV-vis
Results
Results
Results
Ping-pong mechanism
Results
Electron transfer mechanism
[LUMO]
Results
Results
Conclusion• 1 GO-COOH possesses intrinsic peroxidase-like activity and its catalysis is
strongly dependent on pH, temperature, and H2O2 concentration, similar to HRP.
• 2 In the presence of H2O2 and peroxidase substrate TMB, GO-COOH can produce a blue color reaction. Kinetic analysis indicates that the catalysis follows a ping-pong mechanism, and GO-COOH has even higher catalytic activity to TMB than HRP.
• 3 GO-COOH, as a mimic peroxidase, shows several advantages over natural enzymes, such as ease of preparation, low-cost, and stability.
• 4 Compared to HRP and Fe3O4 nanoparticles, GO-COOH possesses high surface-to-volume ratios as well as high affinity to organic substrates through π-π and hydrophobic interactions.
• 5 We design and develop a simple, cheap, and highly selective and sensitive colorimetric assay to detect glucose in buffer solution or diluted blood and fruit juice samples. Our work will facilitate the utilization of GO-COOH intrinsic peroxidase activity in medical diagnostics and biotechnology.