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    Salmonella -

    Epidemiology, Serotypes Distribution and Drug Susceptibility Testing

    of Salmonella Isolated from Swine farms in Chiang Mai-Lamphun 1 1 2 3 3

    Kittipong Kumpapong1 Danai Sinthuya1 Nattakarn Awaiwanont2 Panuwat Yamsakul3 and

    Prapas Patchanee3

    Salmonella

    Salmonella 12 41.23

    36.84

    Salmonella Norfloxacin Ampicillin

    : , , , Salmonella

    Abstract The purpose of this study was to determine prevalence, quantification and drug susceptibility testing

    of Salmonella contamination in swine farms. Pigs fecal and environmental samples were collected from pigs

    farms. Then all samples were cultured in a laboratory for Salmonella detection using conventional

    microbiological method. The findings revealed that, the highest Salmonella prevalence was demonstrated in

    pigs aged 12 weeks at 41.23 percent. From environment samples, boot sample was found to contain the

    highest prevalence at 36.84 percent. Salmonella showed the highest susceptibility for Norfloxacin and the

    highest resistant Ampicillin.

    Keywords: Epidemiology, Drug susceptibility, Serotypes distribution, Salmonella

    1 2 | 3 1Master Student in Veterinary Science, Faculty of Veterinary Medicine,Chiang Mai University. Email: 541431010@cmu.ac.th 2Researchers, Faculty of Veterinary Medicine, Chiang Mai University. Email: a.nattakarn@gmail.com 3Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University. Email: patprapas@gmail.com

  • 3

    Salmonella [1] ..2555 Salmonella 87,557 [2] Salmonella [3] Salmonella [4]

    Salmonella Salmonella Salmonella Salmonella 20 [5] 12.3 [6] 28 [7]

    Salmonella Salmonella

    Salmonella 70 Salmonella 101-103 Salmonella Salmonella Salmonella Salmonella

    Salmonella 2,579 [8] Salmonella Salmonella Enteritidis Salmonella Typhimurium [9] Salmonella [10] Salmonella Weltevreden [11,12]

    Salmonella Salmonella (Multi-drug resistant) Salmonella [13-15]

  • 4

    Salmonella Salmonella -

    (Cross-sectional study)

    Salmonella 6 830 642 3, 8, 12, 18 24 188

    100 Buffered Peptone Water (BPW) 1:10 25 Swab Technique 100 4-8 24

    ISO 6579:2002, Amendment 1:2007, Annex D

    Salmonella Most Probable Number (MPN) 3 25 Buffered Peptone Water (BPW) 225 1:10 Stomacher 2 37 24 100 Modified Semi-solid Rappaport-Vassiliadis (MSRV) 42 24 MSRV Xylose Lysine Deoxycholate XLD agar Brilliant-green Phenol-red Lactose Sucrose BPLS agar 37 24

    100 BPW 1:10 37 24 100 Rappaport-Vassilliadis RV broth 10 1 Tetrathionate TT broth 9 42 24 RV broth 37 24-48 TT broth RV TT broth XLD agar BPLS agar 37 24 Salmonella WHO: The National Salmonella and Shigella Center

  • 5 Salmonella

    95 (95% Confident Interval) PHStat 2.7 (Add-In for Microsoft Excel 2010) (Average Log10MPN/g) Salmonella @Risk 5.5 (Palisade Corporation 798 Cascadilla Street Ithaca, New York 14850 U.S.A.)

    1 Salmonella

    Type of sample Prevalence (%(n)) 95% Confident

    Interval Average Log10MPN/g

    95% Confident

    Interval

    Sow 19.79 (19/96) 11.82-27.76 2.07 1.47-4.04

    Boar 21.21 (14/66) 11.34-31.07 2.45 1.86-2.96

    3 weeks old 10.41 (10/96) 4.30-16.52 1.97 1.47-2.72

    8 weeks old 10.52 (17/95) 4.35-16.69 1.69 1.47-1.96

    12 weeks old 41.23 (40/97) 31.44-51.03 2.08 1.47-3.46

    18 weeks old 25 (24/96) 16.33-33.66 2.02 1.47-3.66

    24 weeks old 23.95 (23/96) 15.42-32.49 1.98 1.47-3.38

    Feed 0 0 0 0

    Water 0 0 0 0

    Feeder 13.33 (4/30) 1.16-25.49 2.45 1.55-3.38

    Nipple 16.66 (5/30) 3.30-30.00 2.45 1.55-3.38

    Floor 16.66 (5/30) 3.30-30.00 2.45 1.55-3.38

    Worker hand 0 0 0 0

    Worker boot 36.84 (7/19) 15.15-58.53 2.45 1.55-3.38

  • 6 2 Salmonella

    (%) Ampicillin 92.3

    Amoxicillin-clavulanic acid 5.7 Chloramphenicol 24

    Ciprofloxacin 1.9 Cefotaxime 6.7

    Nalidixic acid 0.9 Norfloxacin 0

    Streptomycin 88.5 Tetracycline 80.76

    Sulfamethoxazole-Trimethoprim 45.19

    3 Salmonella

    () (%) Salmonella Rissen 34.61 Salmonella Typhimurium 29.80 Salmonella Anatum 8.65 Salmonella Weltevreden 5.76 Salmonella I. 4,5,12:i:- 5.76 Salmonella Panama 4.80 Salmonella Lexington 4.80 Salmonella Weltevredenvar 15+ 2.88 Salmonella Augustenborg 0.96 Salmonella Krefeld 0.96 Salmonella I. 4,12:i:- 0.96

    100

    Salmonella 12 41.23 18 24 25 23.95 ( 1) 36.84 16.66 ( 1) Salmonella Norfloxacin (NOR) 100 Salmonella 3 Ampicillin (AMP) 92.3 Streptomycin (S) 88.46 Tetracycline (TE) 80.76 ( 2) Salmonella Salmonella Rissen 34.61 Salmonella Typhimurium 29.8 Salmonella Anatum 8.65 ( 3)

  • 7

    12 41.23 ( 1) 2.08 Log10MPN/g Garca-Feliz et al (2009) Salmonella 44 [16] 18 24 25 2.02 Log10MPN/g 23.95 1.98 Log10MPN/g Salmonella Salmonella 28 [7] Salmonella Salmonella 29 [17] Salmonella 36.84 2.45 Log10MPN/g Salmonella Dorn-In et al (2009) 38.6 [18] 19.79 21.21 ( 1) ( 3 8 ) Salmonella [19] 13.33 16.66 Julie Funk (2004) [19]

    Salmonella (Biosecurity system) Salmonella

    Salmonella Salmonella Rissen 34.61 Salmonella Typhimurium 29.8 Salmonella Anatum 8.65 ( 3) Salmonella Norfloxacin Ampicillin Streptomycin Tetracycline

  • 8

    Salmonella 12, 18 24 Salmonella Salmonella

    () ( P-10-10409) WHO: The National Salmonella and Shigella enter Salmonella

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  • 9 11. Bangtrakulnonth A, Pornreongwong S, Pulsrikarn C, Sawanpanyalert P, Hendriksen RS, Lo Fo Wong

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    19. Julie Funk WAG. Risk factors associated with Salmonella

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