The extraction and separation of

chlorophyll (α+β) carotin and

xanthophyll in fresh green leaves

Eugene Ong | eugene@usm.my Institute for Research in Molecular Medicine Universiti Sains Malaysia

27 June 2014 SMK Penang Free

Plant Physiol. Apr 1928; 3(2): 211–216.

Plant Physiol. Apr 1928; 3(2): 211–216.

Thin-Layer Chromatography

lutein (yellow) chlorophyll a (yellow – green)

chlorophyll b (dark green)

β-carotene (yellow)

pheophytin (brown – green)

xanthophylls

STATIONARY PHASE Plate coated with silica.

LEAF EXTRACT

MOBILE PHASE Solvents (acetone, alcohol, hexane, etc)

x y Retention value,

Rf = x y

Thin-Layer Chromatography

STATIONARY PHASE Plate coated with silica.

LEAF EXTRACT

MOBILE PHASE Solvents (acetone, alcohol, hexane, etc)

1938, Ukraine

Acetone, 1610, France

Chlorophyll, 1817, France

Thin-Layer Chromatography

SCIENCE & TECHNOLOGY

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1953 – James Watson and Francis Crick discovered the structure of DNA.

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