Conventional and molecular surveillance of paratuberculosis in

Tamil Nadu

Conventional and molecular surveillance of paratuberculosis in

Tamil Nadu

Vinodh Kumar,O.RDivision of Epidemiology

ICAR-Indian Veterinary Research InstituteIzatnagar, Uttar Pradesh, India

A1 , B1- negative controlC1, D1 – positive control

A1, A2- positive control

B1, B2 – negative control

Gamma interferon assay (Bovigam,Biocor,USA)

A1 , B1- negative controlC1, D1 – positive control

Out of 1034 sera samples tested 126 (12.24 % ) were positive to MAP IgG antibodies.

ELISA on 486 samples which represented Chennai and sub urban areas showed 78 were positive (16.04%).

Seropositivity in 460 sheep brought for slaughter at the local abattoir showed 44 (9.56%) were positive by ELISA

Districts Number of

samples

PCR Positives Percentage

Salem 220 54 24.55

Karur 37 4 10.81

Namakkal 35 6 17.14

Dharmapuri 41 5 12.20

Krishnagiri 28 4 14.29

Erode 82 15 18.29

Trichy 141 17 12.06

Perambalur 67 15 22.39

Ariyalur 25 7 28.00

Dindigul 72 8 11.11

Madurai 156 31 19.87

Thanjavur 37 4 10.81

Coimbatore 93 10 10.75

1034 180 17.41

Calvings Number

of

samples

ELISA Positivity

%

PCR Positivity

%

AFB Positivity

%

Heifer 32 14 43.75 21 65.63 17 53.13

First calving 87 19 21.84 24 27.59 4 4.60

Second

calving

527 57 10.82 92 17.46 38 7.21

Third calving 308 28 9.09 55 17.86 28 9.09

Fourth

calving

64 12 18.75 2 3.13 4 6.25

Fifth calving 16 9 56.25 6 37.50 0 0.00

Calvings vs percentage positivity

Districts Number of

samples

AFB positives Percentage

Salem 220 20 9.09

Karur 37 2 5.41

Namakkal 35 1 2.86

Dharmapuri 41 2 4.88

Krishnagiri 28 1 3.57

Erode 82 7 8.54

Trichy 141 12 8.51

Perambalur 67 5 7.46

Ariyalur 25 3 12.00

Dindigul 72 4 5.56

Madurai 156 12 7.69

Thanjavur 37 2 5.41

Coimbatore 93 8 8.60

1034 79 7.64

Districts Number of

samples

ELISA

positives

Percentage

Salem 220 40 18.18

Karur 37 2 5.41

Namakkal 35 3 8.57

Dharmapuri 41 3 7.32

Krishnagiri 28 2 7.14

Erode 82 8 9.76

Trichy 141 15 10.64

Perambalur 67 12 17.91

Ariyalur 25 2 8.00

Dindigul 72 7 9.72

Madurai 156 23 14.74

Thanjavur 37 2 5.41

Coimbatore 93 7 7.53

1034 126 12.19

Thank you