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For Healthy Growth
MAKING LIQUID B. CLAUSII SPORETHE INTRODUCTION
For Healthy Growth
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B. clausii produces useful enzymes amylase, protease and vitamin B.
B. clausii is polyantibiotic resistant to: ampicillin, cephalosporin, erythromycin, lincomycin, neomycin, chloramphenicol, tetracycline …
B. clausii is a safe bacterium (belongs to General Regarded As Safe, GRAS)
The characters of B. clausii
For Healthy Growth
Heat resistance of B. clausii spore. The spores were suspended in PBS buffer and incubated at various temperatures for 20 minutes before viable count. A half of the viable count at room temperature is remained at 90oC.
The time course of B. clausii spore stored at room temperature. More than half of the viable count is remained after 2 years.
Acid resistance of B. clausii spore. The spores were suspended in low pH buffers to mimic the pH of intestinal tract for 8 hours. The remained living spores were estimated by viable count. More than half of the viable count is remained at pH 2.
The Stability of B. clausii spore
Data were done by KLEPT
For Healthy Growth
High stability in hot drinks up to 60oC:
could be used together with hot milk,
juice…
Long shelves-life for 2 years at even up to
40oC
Could be used together with antibiotics
Convenient use for babies and children
Competitive price
The advantage of liquid B. clausii spore
For Healthy Growth
The certificate for service of R&D and QC granted by the Key Laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science
The GMP certificateThe FDA certificate
The CERTIFICATE
For Healthy Growth
THE PRODUCT WAS MADE BY UNIVERSITY SPIN OFF COMPANY ANABIO R&D JSC
CEO: Nguyen Hoa Anh, PhD in Life Sciences15 years studying and working in Tohoku University, Japan
QC Manager: Nguyen Thi Mai Phuong PhD in Biochemistry4 years postdoctoral research associate, University of Rochester, NY, USA
R&D Manager: Nguyen Thi Van Anh PhD in Life Sciences 5 years for studying in Japan
Financial Manager: Nguyen Thi Hoang Mai, MAAccounting and Finance, University of Leeds, UK
Product Manager: Pham Tien Dat. BSc. in Computer Science, 5 years for studying in Hanoi University of Science and Technology
For Healthy Growth
The Factory structure
OfficeDirectorManagementFinancial, Marketing, Sale Reception
R&D at KLEPT Lab scaleR&D at ANABIO Pilot scale
R&D
Spore Making: Spore Generation, Spore Purification BFS: Full automatic machine of Blowing, Filling, Sealing and Packaging,
QC at ANABIOQC at KLEPT
QC
Production:
For Healthy Growth
The R&D was done in KLEPT
For Healthy Growth
Making B. clausii spore at ANABIO
B. clausii spore maker
For Healthy Growth
Filling, labeling and packing at ANABIO
For Healthy Growth
The quality control was done in KLEPT
For Healthy Growth
The quality control was also done in ANABIO
For Healthy Growth QC
(ANABIO, KLEPT)
Mixing
Sterilized water
Filling
Sampling for analysis*
Packing
Sampling for analysis*
Purified B. clausii spore*
QC(ANABIO, KLEPT, NIN)
QC (ANABIO, KLEPT)
How the quality was control
For Healthy Growth
Sequence of 16S Ribosomal RNA
The assays for quality control
Microscopy observation
Viable count on agar plates
Antibiotic resistant
For Healthy Growth
Available in > 2700 pharmacies in 40 provinces of Vietnam 110 clinics in Vietnam 16 hospitals: Bach Mai, L'Hôpital Français de
Hanoi, Nhi Dong 1 – Ho Chi Minh City, Can Tho city …
The distribution
For Healthy Growth
Export to Pakistan, Philippines, Colombia, Mexico…The Export
For Healthy Growth
L'Hôpital Français de HanoiGood responses from the doctors
For Healthy Growth
Some research papers contributed by ANABIO
For Healthy Growth