1.TRANSCRIPTION Dr. Vijay Marakala, MBBS, MD. Assistant professor BIOCHEMISTRY SIMS & RC

2. TRANSCRIPTION Definition Similarity and Differentiation Basic requirements Stages Post- transcriptional processing 3. TRANSCRIPTION [RNA SYNTHESIS] Synthesis of RNA using DNA as a template 4. TRANSCRIPTION mRNA rRNA tRNA SnRNA 5. TRANSCRIPTION Similarities 3 stages Synthesis occurs in the 53 direction Follows Watson-Crick base pairing Differences Ribonucleotides are used in RNA synthesis Uracil replaces Thymine Primer is not required Only a very small portion of genome is transcribed 6. TRANSCRIPTION BASIC REQUIREMENTS Template Substrate Enzyme 7. TRANSCRIPTION 8. TRANSCRIPTION ATP GTP CTP UTP 9. TRANSCRIPTION - Basic requirements -ENZYME DNA dependent RNA polymerase or RNA polymerase Three RNA polymerase RNAP-I RNA-II RNA-III EUKARYOTIC Single RNA polymerase PROKARYOTIC 10. TRANSCRIPTION PROKARYOTIC RNA POLYMERASE 11. TRANSCRIPTION - STAGES INITIATION ELONGATION TERMINATION 12. TRANSCRIPTION STAGES: PROMOTOR REGION 13. TRANSCRIPTION STAGES: 14. TRANSCRIPTION STAGES: 15. TRANSCRIPTION STAGES: 16. TRANSCRIPTION STAGES: 17. TRANSCRIPTION-STAGES: 18. TRANSCRIPTION-STAGES: 19. TRANSCRIPTION-STAGES: 20. TRANSCRIPTION-STAGES: Rho- dependent termination Rho- independent termination 2 mechanisms 21. TRANSCRIPTION-STAGES: 2 mechanisms 22. TRANSCRIPTION-STAGES: 2 mechanisms 23. TRANSCRIPTION IN EUKARYOTES Main differences RNA Polymerase Promoter sites Enhancers, Silencers and TF 24. TRANSCRIPTION IN EUKARYOTES RNA polymerase-I RNA polymerase-II RNA polymerase-III EUKARYOTIC RNA POLYMERASE 25. TRANSCRIPTION IN EUKARYOTES 26. TRANSCRIPTION IN EUKARYOTES ENHANCER