م ي د ق ت: ي ض ا ق ل مد ا ح م و ن س ح ن كي م غ ة ي ع د ج ل ا ا لاي ج ل لا ص ف لو ز ع ة ي سسالادىء ا ا ب م ل ا ن م ة ي ع د ج ل ا ا لاي ج ل ا ة ي ح لا ع ل ا ات ق ي9 ب ط ت ل ى ا ل ا
2. : 3. 4. Differential centrifugation * * * Density gradient
centrifugation 5. Density gradient centrifugation * . 6. :
rate-zonal centrifugation discontinuous Isopycnic centrifugation
continuous Density gradient centrifugation 50 30 10 7. Density
gradient centrifugation particles are separated solely on the basis
of their density Isopycnic = same density 8. * : 9. Mesenchymal
Stem cell Adipose-derived stem cells: Isolation, expansion and
differentiation 10. Mesenchymal Stem/Stromal Cells (MSC) 11.
Mesenchymal Stem: 12. Mesenchymal Stem cell Adipose-derived stem
cells: Isolation, expansion and differentiation 13. : . 14.
Membrane Filtration CD34 enrichment 15. Affinity-based techniques :
Fluorescence Activated Cell Sorting (FACS) Immunomagnetic Cell
Separation marker cluster of differentiation CD 16. Surface
molecular marker 17. 18. Fluorescence Activated Cell Sorting (FACS)
: 19. Immunomagnetic Cell Separation Magnetic-activated cell
sorting (MACS) 20. . : - : 21. ATPS Aqueous Two-Phase Systems
(ATPS) polymerpolymer and polymersalt systems. Most commonly used
polymers include : polyethylene glycol (PEG) and dextran salts are
phosphates, sulfates, and citrates 22. defined by their
physicochemical properties such as hydrophobicity, size, and net
surface charge . 23. based on cellular morphological and
biophysical differences such as mass, charge, size, density, shape,
and rigidity. FFF is achieved within an empty capillary channel by
the combined action of a transporting laminar flow of mobile phase
and a field that is applied perpendicularly to the flow Field Flow
Fractionation FFF 24. ATPS Field Flow Fractionation FFF ATPS Field
Flow Fractionation FFF
