HOW DID OUR ANTIBODY PERFORM?

ANTIBODY CUSTOMER REVIEW: mTOR Polyclonal Antibody (STJ94280)

What was used?Primary antibody: mTOR Antibody (STJ94280)

Provider: St John's Laboratory

Dilution ratio: 1:500

Application: Western Blot

Materials for validation: HepG2, HeLa, C6 and NIH3T3 cell lines

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What was the protocol?

Primary Antibody Application 1:500 primary antibody was incubated at 4˚C for overnight.

Secondary Antibody Application Goat secondary antibody at 1:5000 for 1 hour at room temperature.

Visualisation ECL kit. Exposure time 1 min.

What were the results?

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