HemaCare

BioResearch Products

Viability & Function Data

January 26, 2015

Human-derived Primary Cells

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Peripheral Blood

•Leukopaks

•Platelets

•Whole Blood

•Plasma

•Serum

•Mononuclear Cells

•CD3 Pan T Cells

•CD4 Helper T Cells

•CD8 Cytotoxic T Cells

•CD14 Monocytes

•CD19 B Cells

•CD4/CD25 Regulatory

T Cells

•CD56 NK Cells

•Dendritic Cells

•Macrophages

•Neutrophils

Mobilized Peripheral Blood

•Mononuclear Cells

•CD14 Monocytes

•CD34 Stem Cells

•CD34 Depleted

Mononuclear Cells

•CD133 Stem Cells

Bone Marrow

•Unprocessed Bone Marrow

•Mononuclear Cells

•Mesenchymal Stem Cells

•CD34 Stem Cells

•CD34 Depleted

Mononuclear Cells

•CD133 Stem Cells

Cord Blood

•Unprocessed Cord Blood

•Mononuclear Cells

•CD3 Pan T Cells

•CD4 Helper T Cells

•CD8 Cytotoxic T Cells

•CD14 Monocytes

•CD19 B Cells

•CD34 Stem Cells

•CD133 Stem Cells

•CD56 NK Cells

•Neutrophils

•Mesenchymal Stem Cells

•Plasma

•Dendritic Cells (CD34

derived)

Leukopaks (Fresh or Cryopreserved)

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n = 22

High-quality Leukapheresis Raw

Material Collected at HemaCare Count (x106) ± SD

Total Nucleated Cells 14,400 550

Mononuclear Cells 12,100 600

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n= 1,202 leukapheresis products

Longevity Study - Leukopak

Actual Viable Cell Count in Autologous Plasma

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Peripheral Blood Mononuclear Cells (PBMCs)

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Sources for Mononuclear cells

• Peripheral blood

• Mobilized peripheral blood

• Bone Marrow

• Cord blood

n = 20

Cryopreserved PBMC Functional Assay:

CMV Culture

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INF-γ and TNF-α intracellular staining on day 6 PBMCs stimulated with CMV peptide

T-Cells

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Sources for T-Cells

• Peripheral blood

• Mobilized peripheral blood

• Bone Marrow

• Cord blood

n = 6

National Institute of Allergy and Infectious

Diseases (NIAID)

CSFE Labeled CD3+ Pan T-cells

Cultured with Dendritic Cells:

Functional Assay

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CFSE labeled T cells stimulated

by monocyte derived DC after 5

days undergo multiple divisions.

Ki67 and CD25 expression on T

cells stimulated by monocyte

derived DC after 5 days.

Natural Killer (NK) Cells

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Sources for NK Cells

• Peripheral blood

• Cord blood

n = 7

0

20

40

60

80

100

Pre-Cryo Post-Thaw

% Purity

96.3% 93.0%

NK Functional Assay

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A: Unstimulated

B: +K562 cells

C: +221 cells

A

B

C

B-Cells

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Sources for B-cells

• Peripheral blood

• Cord blood

http://www.bioquicknews.com/node/482

Monocytes

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Sources for Monocytes

• Peripheral blood

• Mobilized peripheral blood

• Cord blood

n = 6

Macrophages (M1 and M2, monocyte derived)

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n = 5

Source: National Cancer Institute (NCI); Creator: Sriram Subramaniam

Macrophages M1

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Type 1-Macrophages (M1) were derived from purified Monocytes. CD14 monocytes were positively selected via

immunomagnetic beads and were cultured in the presence of GM-CSF for 10 days.

Macrophages M2

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Type 2-Macrophages (M2) were derived from purified Monocytes. CD14 monocytes were positively selected via

immunomagnetic beads and were cultured in the presence of M-CSF for 10 days.

Dendritic Cells

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Sources for Dendritic Cells

• Peripheral blood

• Cord blood

Quality data on next slide

Source: National Cancer Institute (NCI); Creator: Sriram Subramaniam

Dendritic Cells – Certificate of Analysis

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Dendritic Cells (DC) were derived from purified CD14+ Monocytes. CD14+ cells were positively selected via

immunomagnetic beads and cultured in the presence of GM-CSF and IL-4 for 7 days

Neutrophils

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Sources for Neutrophils

• Peripheral blood

• Cord blood

Source: National Institute of Allergy and Infectious Diseases (NIAID)

Stem/Progenitor Cell Products

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Sources for Stem/Progenitor Cells

• Mobilized peripheral blood

• Bone Marrow

• Cord blood

n = 5

Mesenchymal Stem Cells

21

Cytotherapy (2006) Vol. 8, No. 4, 315-317

Questions & Answers

Thank You!

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