15 making the complex...simple iggy kass - waters

©2012 Waters Corporation 1

Making the Complex Simple:

UPC2

UltraPerformance Convergence Chromatography

Expanding Selectivity for the

Chromatographic Laboratory


Evolution of Separation Technology

Gas Chromatography Liquid Chromatography Convergence Chromatography

GC

Capillary GC

HPLC

UPLC

SFC

UPC2


Separation Technology Overview

Gas Chromatography

Liquid Chromatography

Convergence Chromatography

Separation achieved by a temperature gradient

•High efficiency [N]• Virtually no limitation on column length

•Limited selectivity [α]• Limited stationary phase options

Separation achieved by a solvent gradient

•High efficiency [N]• Limited to pressure drop across column

• Moderate selectivity [α]• Different modes: reversed-phase, normal-phase, SEC, IEX, affinity, ion

pair, HILIC, GPC…etc.

Separation achieved by density/solvent gradient

•High efficiency [N]• Very low viscosity enables longer columns and smaller particles

•High selectivity [α]• Wide variety of stationary phase and mobile phase co-solvent and

modifier options

GC

LC

CC


UltraPerformance Convergence Chromatography

Convergence Chromatography is a category of separation science that provides orthogonal and increased separation power, compared to liquid or gas chromatography, to solve separation challenges.

UltraPerformance Convergence Chromatography [UPC2] is a holistically designed chromatographic system that utilizes liquid CO2 as a mobile phase

to leverage the chromatographic principles and selectivity of normal phase chromatography while providing the ease-of-use of reversed-phase LC.

The ACQUITY UPC2 System is built utilizing proven UPLC Technology to enable scientists the ability to address routine and complex separation challenges while delivering reliability, robustness, sensitivity and throughput never before possible for this analytical technique.


The Advent of Convergence Chromatography

Convergence Chromatography

UltraPerformance Convergence Chromatography is the result of significant technological advancements in Supercritical Fluid Chromatography instrumentation and chemistry design while providing exceptional increases in available selectivity

SFC UltraPerformance Convergence Chromatography

Data courtesy of Davy Guillarme, Jean-Luc Veuthey LCAP, University of Geneva, Switzerland


Diverse Applicability of Convergence Chromatography

A

B

C

DE

F

H

GI

A 4-hydroxyacetophenoneB p-nitrophenolC 2-hydroxyacetophenoneD 4-chloroacetanilideE acetanilideF phenacetinG 3-aminophenolH paracetamolI 2-aminophenol

Pharmaceutical Impurity Profile

Environmental Food and Beverage

Chemical Materials

Non-ionic surfactantsParacetamol and impurities

Chiral pesticides Vitamin E isomers

Permethrin


Convergence Chromatography:Applied to Neutral and Polar Lipids

PC

SMLPC

PE

TAG

Neutral and Polar Lipids

Unlike RPLC and GC which can only analyze lipids within a single class, UPC2 provides a single methodology to separate complex lipids by class

Exceptional separation of inter and intra class moieties

Selective for a number of biological extracts


TAG mix

CE mix

FFA

All mix

Convergence Chromatography:Applied to Neutral Lipids

ESI-

ESI+


Conclusion

ACQUITY UPC2 Technology, using compressed carbon dioxide (CO2) as the

primary mobile phase, is a separation tool that solves both routine and complex chromatographic problems, especially for samples possessing a wide range of polarities

UPC2 offers scientists unique workflow, application, and environmental impact benefits compared to LC and GC platforms

Because UPC2 is built utilizing UPLC Technology, customers are assured of its optimized performance through holistic design of instrumentation, detectors, software data systems and chemistries.

UPC2 provides an exceptional increase in available selectivity, making this technology widely applicable to a diverse range of compound types

– 80-85% overlap of compounds that can be analyzed by CC and RPLC

– Any compound soluble in an organic solvent


ACQUITY UPC2 System