An Introduction to the Sydney University Proteome Research Unit Dr Ben Crossett

An Introduction to the An Introduction to the Sydney University Sydney University Proteome Research UnitProteome Research Unit

Dr Ben CrossettDr Ben Crossett

The History… • Opened in July 2004 with $2m

MNRF grant & USyd funding• Centre of excellence for

proteomics• Level 2, Building G08• People come from: SMB, SoBS,

Medicine, VetSci, Agriculture, • ...as well as: MQ, UNWS, UWS,

UTS, Brisbane, UK, USA, Germany etc...

To gel or...

...or not to gel

The Results…

• Whole protein mass• Identify proteins• Labelling QC• Changes in protein

abundance, e.g. DIGE or iTRAQ

• Mapping post-translational modifications

• MS ‘western blots’

2000.0 7600.2 13200.4 18800.6 24400.8 30001.0

Mass (m/z)

1.2E+4

0

10

20

30

40

50

60

70

80

90

100

% In

ten

sity

Voyager Spec #1[BP = 23504.3, 12073]

23505.27

23452.36

23401.56

23585.13

23622.9023659.68

23700.26

23744.51

11742.36

23319.85

2014.3623799.20

2280.91

3014.85

2466.6411846.38

2362.002856.22

23162.722979.573832.54 11936.27

22607.404491.42

5796.63 25204.0117286.42

Accessing SUPRU • Contract research: samples

analysed for fixed fee • Research hotel: come and work

in SUPRU • Collaborative research projects• Short courses &

under/postgraduate lectures

• Contact us at:sydney.edu.au/proteomicsben.crossett@sydney.edu.au