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attL1. Reporter. attL2. attL4. Promoter. attR1. attR2. Terminator. attL3. Promoter Entry Clone. Reporter Entry Clone. Terminator Entry Clone. +. +. Kan R. Kan R. Kan R. +. attR4. Cm R - ccdB. attR3. Destination Vector (Amp R Cm R ccdB + ). Amp R. LR clonase. attB4. - PowerPoint PPT Presentation
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attR4 CmR-ccdB attR3
AmpR
Destination Vector(AmpR CmR ccdB+)
attL4 Promoter
KanR
attR1
Promoter Entry Clone
attL1 Reporter
KanR
attL2
Reporter Entry Clone
attR2 Terminator
KanR
attL3
Terminator Entry Clone
+
LRclonase
attP4 CmR-ccdB attP3
AmpR
Expression Clone(AmpR)
attB4 Promoter attB1 Reporter attB2 Terminator attB3
Plasmid By-product
+
+ +
kanR attP1 attP2kanR kanR
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