עבודת סיום וירולוגיה

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a summary of viruses and treatments for viral infections - in hebrew

Text of עבודת סיום וירולוגיה

Herpes Viruses: dsDNA. +( spikes ). . . . " ' . ( ' )HIV , , (, , UV') -, . :,, o , (, ,CNS ). 1( HSV ), 2( HSV ) -( VZV + ). o (7-3 ), ' - . .CMV - o , B. EBV , 5 -, . 5 HHV . )HSV (Herpes Simplex Virus , ( / - ). . 2 HSV " 1 .HSV primorg recurrent labialis . . , . DNA , 2 .tegument (002 .)Kb- spikes . ( ) -.nuclear pores )-TIF (trans inducing factor . )VHS (virion host shutoff mRNA " mRNA. HSV ,, immediate earlyo -TIF TF. TF '. earlyo DNA pol, TF ' '. lateo '. - DNA - .rolling circle . , scaffold . , . -DNA .

. ( ). mRNA . . , , ( ). - S . ( ). ( ) -.spikes , , TF , - -TIF . LAT (latency .)associated transcript .)UL, US (long, short LAT ICPO ( .) - mRNA - - LAT . - LAT "" (, -). LAT - . - ,UV TF . , . : ' . 2- HSV , . , . ' . %51, . ( 1). ' . (= ) . ' , ( ) ' . acyclovir - .CNS . Acyclovir ,DNA . . , . , DNA pol - DNA, ( ). , .,,lat DNA -. DNA - , . () . )Varicella Zoster Virus (VZV . . . .

- ,HSV , " ". - DNA .HSV - ,HSV , ( , , -01 02 ). , ( ). , . ' " . 2/1- HSV- VZV " . - VZV . - HSV .VZV - VZV -( HSV , ). " . : o . o . .I o ' " .II , . " -01 . . ' ' . . . -21 ' : o . o . . o ' . o . , , - VZV . ( VZV) , . , - ,HSV ' . ( , ) -. RNA, . , - " . - . ' . ( - booster). ( ) -. - ( ' ). - . , "" , . , . . - (-). " - . " . - ( ). ", .

Ramsey-hunt syndrome - zoster . , (), , , . , " .VZV Bell's palsy " 1- HSV , , 7. - " . ' %89 . . ' . ( 3 ) : 1.. 2. VZIP /. 3. VZIG ". (1 , 3 ). HCMV- Human Cytomegalo Virus , (042 .)kbp . - , (01-7 ). . , . " PCR " . . ( ). . , " . " . CMV mononucleosis : , , , , (), (), . (, , , , , ,CSF, , ). ( ). . (= ). . ". -, . - . ( " , ' ). . . - ( ) . . -, %2.0 - CNS. , -. -%08-06 CV. %2.2 - - %2.0 - CNS. : . -:CMV o, o . . o o o . o o . o " PCR , , . , -

CMV . " ( )Avidity ( = ). , . , . - gancyclovir . - foscarnet - DNA ( ). ( ) . ' : " 1- MHC- ER" .3US 2 US-11 US . 6 US . - immediate early protein CTL . 2 US 2- MHC 441 CD4. UL -04 TNF. UL .NK EBV Epstein Barr Virus ( , .)B -infectious mononucleosis , nasopharyngeal carcinoma , Burkitt's .lymphoma - .S DNA . '' , . , ( )restricted , ' . RNA, EBV .RNA B , . ' , , ' , . - B" , , . , - EBV , . EBV ' ( B" C3d ' ). - B . B , . . . " , " ' 6-501/1, . : - EBNA LMP T. - ". . - . . Burkitt's Lymphoma , " . - B .

( ) , . - . -Nasophayngeal Carcinoma , , - . Infectious Mononucleosis , " , . , ,B " " ' , , . , . . " -. . 05-03 , . -01 , 4-2 ( - ). Oral Hairy Leukoplakia , / . .

: 1) (-, ') 2): . ' . . 3) (" , ) 4) ( )HSV, HIV, HPV 5) ( ) ' . : " . DNA ,RNA ( )ds (.)ss . RNA ( )+RNA - ,mRNA ( )-RNA antisense- RNA . " , , . : 1) Acute , Rhinovirus, Rotavirus 2) Persistent , Lymphocytic choriomeningitis Virus 3) Latent , , .HSV 4) Slow HIV .Measles SSPE : 1) DNA- ,RNA .. 2) . 3) , . 4) . 5) , . 6) , . . : 1) ( ) 2) 3) (.)uncoating 4) mRNA 5) 6) 7) mRNA 8) " .

: 1), 2)pH 3) UV 4) , , , .HCl , '. ": 1) " ,PFU, FFU 05.LD50, TCID 2) ' " OD - .EM

: 1) . (, ), (, ,)CSF , (, , ) . ( )CPE" , " . " ,ELISA (, PCR, LCR' ). 2) " , , , ( ). False Positive " RF . . -.CSF

15 , 5 , C-G. dsDNA -042 , . , . ( , " ).

, . . , . . ,inclusion bodies . , . 4 " , 601/1- 01/1. . ' (. ), ( ), ' . / ( , ). . ( -) " 6,5,2,1 , ' . ' . 04 -14 -.GI 4 -7 - acute respiratory distress . . " . . 5 pentone base .fiber mRNA . , . , . , , . - fiber knob " , . -receptor mediated endocytosis . , DNA ( ) . Early events . , . - early . immediate early a " . Immediate early b . - mRNA ( )TF .S ' ( ). DNA-pol ( - mRNA -RNA-pol ). , " - RB-35 p . - mRNA .

RNA vaRNA - . Replication & Late events , - .Late mRNA . leading- .lagging . . terminal protein - DNA . . . . - early - .late mRNA dsRNA " -. - " ( E1 - TF -INF ) -( vaRNA dsRNA PKR. INF -dsRNA . PFU .INF - vaRNA PFU INF .INF 3 E " CTL-.TNF E1A - early pRB -.S E1B 35 p . - early - late , .

RNA RNA RNA+ (RNA , )mRNA -RNA (RNA , -RNA ). RNA ( )Ambisense +RNA ,-RNA - RNA - DNA - .mRNA RNA " . " . " - - mRNA . -RNA , -.Influenze A "(+) - picornaviridae " -. . - - -, -, -. . : "(+) .ss ( ). 5' VPg 3' poly-A " . VPg - ,poly-A 3'. -

VPg - polyprotein . " . , . " leading sequence , "" . , " " IRES (intra-ribosomal )entry site . salk - sabin ( ). ( ) , - sindbis -.togaviridae "(-) VSV-vesicular stomatitis virus " " " . 84 . " . '3 . , " " . " N " . N " . Orthomyxoviruses (" " " , ). ( )reassortment ' . - . ( )S, L " , S " . .MMLV, HIV, HTLV MMLV " , ,ss direct repeats- UTR '3 -'5. ( RT " ") ( " ). tRNA - .RT , . ". " ( , ) . "" . ( HIV) - .S " "" .

DNA

" - -, " , . . ( .)Pox , " " . , origin, . " ,ds " " IE " . " . , . " ,ss . " . , 04- :SV " - . " )TAG (T antigen . " . . , . 3 ( ) . , . " " , " , .S . . , - ,S . Rb .S 35 p . BAX .

Picorna " (42-81 .)nm -4 - : o ( - pH ' , -.)GI , , . o- o o- ( ' , ' ' ). : " (+) 007 . ( ).

-: . (), (), ( ,)A ( A), ( ,)B ( ,B). " - . ' ' . . - ,A A B . : 3 , . . -, %8-5 , , , , , . . %2-1 CNS . - . , ( ) . - ,BBB () . salk IV . Sabin PO , ( .)IgA .

, , , . 3 1) 2) 3) . ' " (, ,)HIV () ' . " , " ' . ' - IFN " " - (" - ). IFN - NFB . - .IFN IFN 5-2 (A)-synthetase DNA ADAR " , ", ". , HSV " - ADAR- HIV " .ADAR ' - TNF . - TNF - TNF . 3 TNF . , TNF , NFB " . (" ) . " " - TNF

-

. ( - Pox) - TNF " , -, TNF . " " ( ). ' - :PKR " IFN .dsRNA -PKR . ( ,EBV, ,HIV) ' dsRNA - PKR , -PKR , . " " , ' . RNA " , -.mRNA " ". , . -" ( )siRNA . , . , .

" ,paramyxoviridae . . , 41-01 . " -, -01 . %59 . , 3-2 , , , ', , . -01 . koplik ,spots . ( ,enanthema ), ( .)exanthema . - - . - , , , ,