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Lab meeting 2014.02.24. Park Hyeri. 2011. CALR - Big dye Purification - Sequencing 2012. CALR , 1~5 - PCR - Agarose gel Electrophoresis Cryo media PubMed 사용법. 2011. CALR. Big dye Purification - Mixture Sequencing 5, 7 mutation. 2012. CALR, 1~5. PCR - PowerPoint PPT Presentation
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Lab meeting2014.02.24
Park Hyeri
• 2011. CALR - Big dye Purification - Sequencing• 2012. CALR , 1~5 - PCR - Agarose gel Electrophoresis• Cryo media• PubMed 사용법
2011. CALR
• Big dye Purification - Mixture
• Sequencing 5, 7 mutation
Big dye reaction PCR Product 10ul
3M Sodium acetate(pH4.6) 2ul
95% EtOH 50ul
2012. CALR, 1~5
• PCR - Mixture - PCR condition
2012. CALR, 1~5
• Agarose gel Electrophoresis : 1X loading dye(1ul) + sample(5ul) : condition – 140V , 35min
Cryo media
RPMI-1640 7 35ml
FBS(비동화 ) 2 10ml
DMSO 1 5ml
Total Vol. 50ml
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