Lab meeting2014.02.24

Park Hyeri

• 2011. CALR - Big dye Purification - Sequencing• 2012. CALR , 1~5 - PCR - Agarose gel Electrophoresis• Cryo media• PubMed 사용법

2011. CALR

• Big dye Purification - Mixture

• Sequencing 5, 7 mutation

Big dye reaction PCR Product 10ul

3M Sodium acetate(pH4.6) 2ul

95% EtOH 50ul

2012. CALR, 1~5

• PCR - Mixture - PCR condition

2012. CALR, 1~5

• Agarose gel Electrophoresis : 1X loading dye(1ul) + sample(5ul) : condition – 140V , 35min

Cryo media

RPMI-1640 7 35ml

FBS(비동화 ) 2 10ml

DMSO 1 5ml

Total Vol. 50ml