NO. 18 都市部花粉アレルゲン特性調査プロジェクト
Preview:
DESCRIPTION
NO. 18 都市部花粉アレルゲン特性調査プロジェクト. プロジェクト参加者 : 森田 淳( M1 )呉 迪( M2 ) 指導教員 : 王 青躍 学外連携組織: 国立環境研究所、廃棄物処理企業、埼玉県環境科学国際センター. - PowerPoint PPT Presentation
Citation preview
PowerPoint Presentation:
In Japan, Japanese cedar (Cryptometria japonica) and cypress
(Chamaecyparis obtusa) pollens are considered to be the major
unique allergens which can give rise to severe pollinosis and their
extent of dispersal is quite wide, especially in the urban area.
Cry j 1 is a causative substance of Japanese cedar pollinosis, and
it may deteriorate by Cry j 1 invasion to a lower respiratory
tract. We observed airborne particles containing Cry j 1 by an
immunofluorescence technique using a fluorescence microscope. We
evaluated a Cry j 1 reacted with various air pollutants by liquid
phase reaction. My study will pay attention to pollen morphological
observation, allergenic measurement of airborne pollens and
cross-antigenicity evaluation in Japanese urban areas, also
including the investigation of various pollens in Chinese urban
areas. Morphological changes of pollens will be observed by an
optical microscope, a fluorescence microscope and a scanning
electron microscope to confirm the existing evidences of visualized
suspended particulate matter containing the Japanese cedar and
cypress pollen allergens.
Abstract
ELISA3-
Fig. 2. The result of One-Side ELISA method (n = 3).
Cry j 11.1 m
5 (< 1.1 m) 3-
: (AHV)
: 566 L/min
: 71
: 1 (> 7.0 m)2 (3.3 ~ 7.0 m)5 (< 1.1 m)
: (AHV)
: 566 L/min
BSA/PBS100 L372()
Tween 20PBS (TPBS)PBS3- (PBS1 : 100) 100 L372
TPBSPBSIgG (PBS1 : 5000)100 L371
TPBSPBSo-100 L30
*
0
1
2
3
4/12-4/184/20-4/26
Polyclonal antibody
Cry j 1 concentration (ng/m
3
3
0
1
2
3
4/12-4/184/20-4/26
Monoclonal antibody cry j 1
0
1
2
3
4/12-4/184/20-4/26
Polyclonal antibody
Polyclonal antibody
Cry j 1 concentration (ng/m
3
3