YPD

YPD

+ Mg 200µM

+ Mn 200µM

MG 200 ng ml-1

Supplementary figure S1

Supplementary figure S1 serial dilution assays to show drug susceptibility of C. albicans to MG (200 ng ml-1) and supplementation with either 200μM Mg or 200μM Mn

Supplementary figure S2

Supplementary figure S2 MG susceptibility screening of 240 TF Mutants were determined at 300 ng ml-1 by serial dilution assay, only repeatedly sensitive strains were picked up for further analysis.

Supplementary figure S3

PG L

am

ount

(n

mol

per

mg

dry

lipid

wt.

)

PG

L co

mpo

sitio

n (%

of n

orm

aliz

ed P

GL

mas

s sp

ectr

al s

igna

l)

Figure S3 Sterols and phosphoglycerides (PGLs) composition under MG treatment as compared with untreated cells. The amount of sterols are represented as % of normalized total sterol mass spectral signal and PGL amount is represented as % of normalized PGL mass spectral signal. Values are means ± SD of 5 independent experiments (n=3). PC: Phosphatidyl choline; PE: Phosphatidyl ethanolamine; PI: Phosphatidyl inositol; PS: Phosphatidyl serine; PG: Phosphatidyl glycerol; PA: Phosphatidic acid;Lyso PC: Phosphatidyl choline; Lyso PE: Phosphatidyl ethanolamine; Lyso PG: Phosphatidyl glycerol

stp2Δ

upc2Δ

Supplementary figure S4

Supplementary figure S4 UPC2 and STP2 have common target, RT-PCR showing log2 ratios of MET6 in stp2Δ and upc2Δ as compared to their respective wild-type strains.