2018319K
TEL 03-3271-8553 FAX 03-3271-7282 TEL 077-565-6969 FAX 077-565-6995 TEL 077-565-6999FAX 077-565-6995Website http://www.takara-bio.co.jpFacebook http://www.facebook.com/takarabio.jp
In-Fusion Cloning Products : This product is covered by the claims of U.S. Patent No. 7,575,860 and its foreign counterpart patent claims.
201841 In-FusionTA
DNAcDNAPCR
cDNA
20184
In-FusionTA
DNADNA
1%150 ml
Agarose L03 TAKARAPrimeGel Agarose LE 1-20K GATPrimeGel Agarose PCR-SieveTris-Acetate-EDTA BufferTAE 50 Powder, pH8.3Tris-Borate-EDTA BufferTBE Powder, pH8.3100 bp DNA LadderDye Plus1 kb DNA LadderDye PlusMupid-2plus Mupid-exU Mupid-One
1 kb
1 kb
PCR1 kb
11 L50 TAE
11 LTBE
1001,500 bp11
110 kb10
CE
100 g100 g100 g110
500 l100500 l100
50035801A5810AT9131T91213422A3426AM-2PEXU-1O1-01
17,00034,00048,00018,00010,00019,00013,00040,76056,95062,000
1.5 g12150 ml 35060
1/1010 Loading BufferDNA
EtBrSYBR Green I
1 253%
2 3%10
3 holder
DNA
500 bp
5005,000 bp
5,000 bp
PrimeGel Agarose PCR-Sieve
Agarose L03PrimeGel Agarose LE 1-20K
PrimeGel Agarose GOLD 3-40K
3%
1%
0.5%
1,5001,000
600
300
100
150200
50
bp
105432
1
kb
19,329
7,7436,2234,2543,4722,690
1,8821,489
925
421
74bp
50 bp DNA Ladder3% PrimeGel Agarose PCR-Sieve
1 kb DNA Ladder1% Agarose L03
EcoT14 I digest1% PrimeGel Agarose LE 1-20K GAT
| 21|
PrimeScript
cDNA
cDNA cDNART-PCR
PrimeScript IIReverse Transcriptase
PrimeScript RT reagentKitPerfect Real Time
PrimeScriptReverse Transcriptase
cDNA
RNAcDNA
RT-PCR
15
PrimeScript RTasecDNA
RNA
13.5 kb 13.5 kb -
42 42 37
3060 3060 15
p. 12
-
p. 34 p. 56
RNaseHmRNAcDNA
4237RNAPrimeScript RTaseRNARNAcDNA
PCR
PCR
1
In-Fusion
Contents
In-Fusion 3 P.1, 2
In-Fusion P.3
In-Fusion P.4
In-Fusion P.5, 6
1 4 P.7 10
P.11, 12
P.13, 14
P.15
2nd EditionIn-Fusion
2015-2016
2015-2016
MACHEREY-NAGEL
2015-2016
MACHEREY-NAGEL
103-8232 215-10TEL.03-3271-8553 FAX.03-3271-7282
525-0058 262TEL.077-565-6969 FAX.077-565-6995
http://www.takara-bio.co.jp
TEL.077-543-6116 FAX.077-543-1977
Facebookhttp://www.facebook.com/takarabio.jp
2014-2015
103-8232 215-10TEL.03-3271-8553 FAX.03-3271-7282
525-0058 262TEL.077-565-6969 FAX.077-565-6995
Facebook
http://www.takara-bio.co.jp
http://www.facebook.com/takarabio.jp
2014-2015
2014-2015
2014630
TEL 03-3271-8553 FAX 03-3271-7282
TaKaRa
TEL 077-543-6116 FAX 077-543-1977
TEL 077-565-6969 FAX 077-565-6995
Website http://www.takara-bio.co.jp
( )
31,500 101,000 24,000 37,000 105,000 31,500 101,000 31,500 101,000 31,500 101,000 30,000 97,000 248,000 30,000 97,000 248,000 29,000 92,000 27,500 88,000 27,500 88,000 29,000 92,000 27,500 88,000 220,000 27,500 88,000 220,000 10,500 33,500 16,000 34,000 109,000 32,000 96,000 31,500 101,000 40,000 136,500 21,000 68,000 52,500 6,700 17,000 63,000 17,000 63,000
R060AR060BR091AR050AR050BR045AR045BR010AR010BRR006ARR006BRR001ARR001BRR001CRR01AMRR01BMRR01CMRR042ARR042BRR002ARR002BRR02AGRR02BGR007AR007BR001AR001BR001CR001AMR001BMR001CMR300AR300BR090ARR062ARR062BR110AR110BR071AR071BR072AR072BR073AR073BR074ARR320ARR300ARR300BRR350ARR350B
250 U 1,000 U A4 100 250 U 1,000 U A4 100 400 A4 250 U 1,000 U A4 250 U 1,000 U A4 250 U 1,000 U 250 U4 3,000 U B3 250 U 1,000 U AM4 3,000 U AM12 125 U 500 U A4 125 U 500 U A4 125 U 500 U AG4 250 U 1,000 U A4 250 U 1,000 U 250 U4 3,000 U B3 250 U 1,000 U AM4 3,000 U AM12 100 400 A4 100 100 400 A4 250 U 1,000 U A4 250 U 1,000 U A4 200 800 A4 40 160 A4 200 160 160 800 A5 160 800 A5
PCR
2016 6
PCRAgarose L03 TAKARA
DL5,000 DNA MarkerPCR EmeraldAmp MAX PCR Master Mix
DNA
NucleoSpin Gel and PCR Clean-up
SYBR Green SYBR Green I DNA SYBR Green II DNA RNA
Mupid
DNA DNA Ladder Ready-to-use Loading Buffer DNA Digested Marker
Agarose TAKARA PrimeGel Agarose
TBE TAE Tris TE
Mupid
DNA
DNAIn-FusionTA3
TT
T
3 A
A 3 BamH I
Hind III
PrimeScript
PCR Enzymes Guide
In-Fusion
In-Fusion
DNA15PCR
50 bp15 kb DNA In-Fusion15
3
TA
Taq DNAPCR3dATA3dTTPCRdADNA5
5
DNADNA
7
T Mighty TA-cloning Kit
PCRTA Mighty TA-cloning Reagent Set for PrimeSTAR
10 kbDNA TaKaRa DNA Ligation Kit LONG
PrimeSTAR DNA PolymeraseDNA35 exonucleasedATAPCR6
5 kbPCR
Mighty Cloning Reagent Set (Blunt End)
Ligation MixDNA DNA Ligation Kit
In-Fusion TA
1
2
1
2
1
2
3
PCR
PCR
PCR
In-Fusion
12In-Fusion
In-Fusion TA
| 21|
PrimeScript
cDNA
cDNA cDNART-PCR
PrimeScript IIReverse Transcriptase
PrimeScript RT reagentKitPerfect Real Time
PrimeScriptReverse Transcriptase
cDNARNAcDNA
RT-PCR15
PrimeScript RTasecDNARNA
13.5 kb 13.5 kb -
42 42 37
3060 3060 15
p. 12
-
p. 34 p. 56
RNaseHmRNAcDNA
4237RNAPrimeScript RTaseRNARNAcDNA
PCR
PCR
1
InInInIn-InInInIn-FusionFusionFusion
Contents
In-Fusion 3 P.1, 2
In-Fusion P.3
In-Fusion P.4
In-Fusion P.5, 6
1 4 P.7 10
P.11, 12
P.13, 14
P.15
2nd EditionInIn-In-Fusion
2nd EditionFusion
2015-2016
2015-2016
MACHEREY-NAGEL
2015-2016
MACHEREY-NAGEL
103-8232 215-10TEL.03-3271-8553 FAX.03-3271-7282
525-0058 262TEL.077-565-6969 FAX.077-565-6995
http://www.takara-bio.co.jp
TEL.077-543-6116 FAX.077-543-1977
Facebookhttp://www.facebook.com/takarabio.jp
2014-2015
103-8232 215-10TEL.03-3271-8553 FAX.03-3271-7282
525-0058 262TEL.077-565-6969 FAX.077-565-6995
Facebook
http://www.takara-bio.co.jp
http://www.facebook.com/takarabio.jp
2014-2015
2014-2015
2014630
TEL 03-3271-8553 FAX 03-3271-7282
TaKaRa
TEL 077-543-6116 FAX 077-543-1977
TEL 077-565-6969 FAX 077-565-6995
Website http://www.takara-bio.co.jp
( )
31,500 101,000 24,000 37,000 105,000 31,500 101,000 31,500 101,000 31,500 101,000 30,000 97,000 248,000 30,000 97,000 248,000 29,000 92,000 27,500 88,000 27,500 88,000 29,000 92,000 27,500 88,000 220,000 27,500 88,000 220,000 10,500 33,500 16,000 34,000 109,000 32,000 96,000 31,500 101,000 40,000 136,500 21,000 68,000 52,500 6,700 17,000 63,000 17,000 63,000
R060AR060BR091AR050AR050BR045AR045BR010AR010BRR006ARR006BRR001ARR001BRR001CRR01AMRR01BMRR01CMRR042ARR042BRR002ARR002BRR02AGRR02BGR007AR007BR001AR001BR001CR001AMR001BMR001CMR300AR300BR090ARR062ARR062BR110AR110BR071AR071BR072AR072BR073AR073BR074ARR320ARR300ARR300BRR350ARR350B
250 U 1,000 U A4 100 250 U 1,000 U A4 100 400 A4 250 U 1,000 U A4 250 U 1,000 U A4 250 U 1,000 U 250 U4 3,000 U B3 250 U 1,000 U AM4 3,000 U AM12 125 U 500 U A4 125 U 500 U A4 125 U 500 U AG4 250 U 1,000 U A4 250 U 1,000 U 250 U4 3,000 U B3 250 U 1,000 U AM4 3,000 U AM12 100 400 A4 100 100 400 A4 250 U 1,000 U A4 250 U 1,000 U A4 200 800 A4 40 160 A4 200 160 160 800 A5 160 800 A5
PCR
2016 6
PCRAgarose L03 TAKARA
DL5,000 DNA MarkerPCR EmeraldAmp MAX PCR Master Mix
DNA
NucleoSpin Gel and PCR Clean-up
SYBR Green SYBR Green I DNA SYBR Green II DNA RNA
Mupid
DNA DNA Ladder Ready-to-use Loading Buffer DNA Digested Marker
Agarose TAKARA PrimeGel Agarose
TBE TAE Tris TE
Mupid
DNA
DNAIn-FusionTA3
TT
T
3 A
A 3 BamH I
Hind III
PrimeScript
PCR Enzymes Guide
In-Fusion
In-Fusion
DNA15PCR
50 bp15 kb DNA In-Fusion15
3
TA
Taq DNAPCR3dATA3dTTPCRdADNA5
5
DNADNA
7
T Mighty TA-cloning Kit
PCRTA Mighty TA-cloning Reagent Set for PrimeSTAR
10 kbDNA TaKaRa DNA Ligation Kit LONG
PrimeSTAR DNA PolymeraseDNA35 exonucleasedATAPCR6
5 kbPCR
Mighty Cloning Reagent Set (Blunt End)
Ligation MixDNA DNA Ligation Kit
In-Fusion TA
1
2
1
2
1
2
3
PCR
PCR
PCR
In-Fusion
12In-Fusion
In-Fusion TA
https://www.takara-bio.co.jp/goods/catalog/pdf/cloning_compact_guide.pdfhttps://www.takara-bio.co.jp/goods/catalog/pdf/prime_web2.pdfhttps://www.takara-bio.co.jp/goods/catalog/pdf/in-fusion.pdfhttps://www.takara-bio.co.jp/goods/catalog/cat4.htmhttps://www.takara-bio.co.jp/goods/catalog/pdf/restriction_enzyme2014-2015.pdfhttps://www.takara-bio.co.jp/goods/ca
