4
ù(úûüÉÊ?´ãýþÿË° È Ð ÑÐ Òq ÓEÔ ÕÖ 1. íî®&X\%&&)íî¬< 610064 2. "uíî"U=<=U=%&'()íî¬< 610072 ./ /0IJ×E÷KHØb0Ù:ÚÛÜQR:;0R£ÝôÙ:ÚÛÜIÞÃß0àáx。[ @AJC ~R £O nÙ:ÚÛÜZâVWRIJ×ru ~ÜnQR:;0R£ãÙ:ÚÛÜ]äåæèh ~xgéê ÞÃßàáëì。[ M\wMüÙ:ÚÛÜÜn]í@ R£_0:6uSPî ~JKR£0:6u= 1.65~7.30 ï]Ý ðí@ _HP 0:6ur 7.13×10 w/mL HP ]èh0åæxgÞÃß<ur= 21.05mg/L Säéê ñò0r¡ÞÃß<u= 1.07 ïS=çñò0r¡ÞÃß<u= 1.64 ï。[ M_ruJKÜnQR:;0R£@ óåæxvèh 10%ÞÃßàáur012 Ù:ÚÛÜÞÃßIJåæx 34567 S-3  89:;< A  8=>7 0517-6611 2018 27-0013-04 MediumOptimizationandInducingConditionsOptimizationof Haematococcuspluvialis LIUKehuan BAIHuan MALi etal 1.CollegeofLifeSciences SichuanUniversity Chengdu Sichuan610064 2.ElectricPower ResearchInstituteofSichuanElectricPowerCompany Chengdu Sichuan610072 Abstract Objective MediumforculturingHaematococcuspluvialis originatedfrom laboratorywasscreened accumulationconditionsof astaxanthinin Haematococcuspluvialis wereoptimizated. Method Sevenculturemediumswereusedtocultivatefourkindsof Haematococcus pluvialis 4kindsofmediumswereobtained.AstaxanthinaccumulationinHaematococcuspluvialis wereexposedunderlightinduced high salt andnitrogendeficientrespectively. Result BiomassaboutfourstrainsalgaeintheformulaAmediumwas165-7.30timesofthatin theothersixmediums HP gainedthelargestbiomassinformulaA themaximumbiomasswasupto7.13×10 cell/mL.Undernitrogendefi ciencycondition theastaxanthinproductionwasupto21.05mg/L thisnumberwas1.07timesofthebestastaxanthinyieldobtainedby light and1.64timesofthemaximumastaxanthinyieldobtainedbyhighsalt. Conclusion Themostsuitable Haematococcuspluvialis medi umwasformulaAandtheregnantastaxanthinaccumulationconditionwas10% inthisstudy. Keywords Haematococcuspluvialis Astaxanthin Mediumoptimization Stressconditions ?@AB "# ñ ¬ ©/ 2014JY0171 ); "# ñ ô  ¡©/ 13H1138 )。 CDEF È Ð1994 —), !õÝ%&:@'ÜÇZǬöbEC¼Î&¶·Ü Çìpb÷Üøù¦úûüGHIJ 2018-07-17 ]g^ßβ β _`a-4 ¥u ôo_`a:sWbcc-vÖs β ms β Hvd:s^ßs¥ß13 s}ef "Be"¨ÂÒ_]gu!ogh1]gùÒ' Cõ=¡ÏYßiçÏä2' 1-3 ]g «oôä2ÙÑAköç|i&:Ã6$Cj SWsd$ÍÎ#k«]g 95%-uC&I¬ö:º 1% ul¦³z 4-6 I¬]gÒ_àf} ÈÊËÃä2oSúû 7-8 -æI¬]gÔ mXË]gUn89ÎnoXDplA<R±Y Ë$!-q¯«XË]g³zoXDpl!] g«X[I¬¹AÄ_rsrcdËÔrs e®l]«ðñXÙ-¥_¤·oXDpl] g_Ç$u¤· t³cd¸cdoXDpl -ðñç¹Al¦X[;ËzcdoXDplr s¹A]goXDpl-ðñXÙ7ï«X[;÷å]g« Ã;-oXDplÉñ«üËu®|oX Dpl]goÿ)«l]uûÅèé6~2 ¤«ølo(Éñß®oXDplX[;«®;¹AoXDpl-ðñ竪ÃÄ]_:(Éñß!- ¥ oXDpl¶Éñß!« pH¨C»ØvÉñ· !Éñ߬2+áwlçÂx[ÝÞÃXÉñß pH8X¨Cl«X[;ÍÎü:'(3 Ã_l ôÉñ· ! à Éñ G ! ¤ ~ 1%~2%« CO CO /air ), ¡:÷ pH¨Cðñ[áwÚ׫lç µúû 10 -d_¤3·!3÷y Û«±ì¤3;ĪAù¯oXDpl_rs·!zÑursÎïl!¹A «]gdoÚÕÖaåoXDpl_rs rÑçÏk8¦_`aC±o_`a\] u]gÑŦ_`a« 80%~99% ), ¦_`a d6²³ 11-12 -¡¤·oXDpl!¦_` a¨C;³PÖíç]g¹A¼½±14[£-þ}.l]ðñXÙ«Éñߪ l!]g«Ã;Z '( [ | ô o X D pl Éñß BBM OHM â y MCM ç Õ BG11 BG11-1 BG11- 21 ), HP HP HP HP ]oXDpll]cd»Ø Éñ±®|4«xÌÉñß_xÌÉñß!ÙÌ -U«l]'(Ì6ù¸ªAï%rsr]g «¹A¼½1 KL'MN 1.1 OPünì?ÉÊ } . l ô o X D pl Haematococcuspluvialis HP HP HP HP u¤·2#p CÙïÑÒ«l]1.2 ÉÊ?´ãuJ$ÉÊ oXDplÉñ\]^_`aJ.AnhuiAgric.Sci.2018 46 27 ): 13-16

ø(œßüÉ˚.ªýþÿ!¸° · theothersixmediums,HP ... ßl|lç—, |}G42‹'vrsŒ„rÉæ,rs Éæ«Öô(‚– 5×105~6×105s/mL,rsÉæ7irs ¤;»,ÑflÉ挄ł¤。

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Page 1: ø(œßüÉ˚.ªýþÿ!¸° · theothersixmediums,HP ... ßl|lç—, |}G42‹'vrsŒ„rÉæ,rs Éæ«Öô(‚– 5×105~6×105s/mL,rsÉæ7irs ¤;»,ÑflÉ挄ł¤。

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MediumOptimizationandInducingConditionsOptimizationofHaematococcuspluvialisLIUKehuan1,BAIHuan2,MALi1etal (1.CollegeofLifeSciences,SichuanUniversity,Chengdu,Sichuan610064;2.ElectricPowerResearchInstituteofSichuanElectricPowerCompany,Chengdu,Sichuan610072)Abstract [Objective]MediumforculturingHaematococcuspluvialisoriginatedfromlaboratorywasscreened,accumulationconditionsofastaxanthininHaematococcuspluvialiswereoptimizated.[Method]SevenculturemediumswereusedtocultivatefourkindsofHaematococcuspluvialis,4kindsofmediumswereobtained.AstaxanthinaccumulationinHaematococcuspluvialiswereexposedunderlightinduced,highsalt,andnitrogendeficientrespectively.[Result]BiomassaboutfourstrainsalgaeintheformulaAmediumwas165-7.30timesofthatintheothersixmediums,HP3gainedthelargestbiomassinformulaA,themaximumbiomasswasupto7.13×106cell/mL.Undernitrogendeficiencycondition,theastaxanthinproductionwasupto21.05mg/L,thisnumberwas1.07timesofthebestastaxanthinyieldobtainedbylight,and1.64timesofthemaximumastaxanthinyieldobtainedbyhighsalt.[Conclusion]ThemostsuitableHaematococcuspluvialismediumwasformulaAandtheregnantastaxanthinaccumulationconditionwas10%inthisstudy.Keywords Haematococcuspluvialis;Astaxanthin;Mediumoptimization;Stressconditions

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Page 2: ø(œßüÉ˚.ªýþÿ!¸° · theothersixmediums,HP ... ßl|lç—, |}G42‹'vrsŒ„rÉæ,rs Éæ«Öô(‚– 5×105~6×105s/mL,rsÉæ7irs ¤;»,ÑflÉ挄ł¤。

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[1]FASSETTRG,COOMBESJS.Astaxanthinincardiovascularhealthanddisease[J].Molecules,2012,17(2):2030-2048.

[2]HANDX,LIYT,HUQ.Astaxanthininmicroalgae:Pathways,functionsandbiotechnologicalimplications[J].Algae,2013,28(2):131-147.

[3]AMBATIRR,PHANGSM,RAVIS,etal.Astaxanthin:Sources,extraction,stability,biologicalactivitiesanditscommercialapplications:Areview[J].Marinedrugs,2014,12(1):128-152.

[4]P?REZL?PEZP,GONZ?LEZGARC?AS,JEFFRYESC,etal.Lifecycleassessmentoftheproductionoftheredantioxidantcarotenoidastaxanthinbymicroalgae:Fromlabtopilotscale[J].Journalofcleanerproduction,2014,64:332-344.

[5]LIJ,ZHUDL,NIUJF,etal.AneconomicassessmentofastaxanthinproductionbylargescalecultivationofHaematococcuspluvialis[J].Biotechnologyadvances,2011,29(6):568-574.

[6]KOLLERM,MUHRA,BRAUNEGGG.Microalgaeasversatilecellularfactoriesforvaluedproducts[J].Algalresearch,2014,6:52-63.

[7]MILLEDGEJJ.Commercialapplicationofmicroalgaeotherthanasbiofuels:Abriefreview[J].Reviewsinenvironmentalscience&bio/technology,2011,10(1):31-41.

[8]PANISG,CARREONJR.CommercialastaxanthinproductionderivedbygreenalgaHaematococcuspluvialis:Amicroalgaeprocessmodelanda

technoeconomicassessmentallthroughproductionline[J].Algalresearch,2016,18:175-190.

[9]BARROSMP,POPPESC,SOUZAJUNIORTP.Putativebenefitsofmicroalgalastaxanthinonexerciseandhumanhealth[J].Revistabrasileiradefarmacognosia,2011,21(2):283-289.

[10]YINSC,WANGJF,CHENL,etal.ThewaterfootprintofbiofilmcultivationofHaematococcuspluvialisisgreatlydecreasedbyusingsealednarrowchamberscombinedwithslowaerationrate[J].Biotechnologyletters,2015,37(9):1819-1827.

[11]DRAGO爦N,BERCEAV,BICAA,etal.Astaxanthinproductionfromanewstrainofhaematococcuspluvialisgrowninbatchculture[J].Annalsoftheromaniansocietyforcellbiology,2010,15(2):353.

[12]SOLOVCHENKOAE,CHIVKUNOVAOB,MASLOVAIP.Pigmentcomposition,opticalproperties,andresistancetophotodamageofthemicroalgaHaematococcuspluvialis,cultivatedunderhighlight[J].Russianjournalofplantphysiology,2011,58(1):9-17.

[13]CHOIYE,YUNYS,PARKJM,etal.DeterminationofthetimetransferringcellsforastaxanthinproductionconsideringtwostageprocessofHaematococcuspluvialis,cultivation[J].Bioresourcetechnology,2011,102(24):11249-11253.

[14]UìV

,Wo¿

,2@X

,°

.ÛYZ[\.]Û%^__`3ôab

&cd

[J].IJef

,2016(8):29-32.[15]

ùgò

,#D

,hij

,°

.k-

、l-

、mnH-9

pH5io\p_

�¹.�F

[J].ioRqr

,2001,32(1):15-18.[16]CORDEROBF,COUSOI,LE?NR,etal.Enhancementofcarotenoids

biosynthesisinChlamydomonasreinhardtiibynucleartransformationusingaphytoenesynthasegeneisolatedfromChlorellazofingiensis[J].Appliedmicrobiology&biotechnology,2011,91(2):341-351.

[17]DEMMIGADAMSB,ADAMSW W.Antioxidantsinphotosynthesisandhumannutrition[J].Science2002,298(5601):2149-2153.

[18]fs

,'>½

,tBu

,°

.]Û%^_vw^xNOyz&].{|

R\}~�

[J].)ÛÛ�CD

,2012,36(4):640-645.[19]KIMJH,AFFANA,JANGJ,etal.Morphological,molecular,andbio

chemicalcharacterization ofastaxanthinproducing green microalgaHaematococcussp.KORDI03(Haematococcaceae,Chlorophyta)isolatedfromKorea[J].JMicrobiolBiotechnol,2015,25(2):238-246.

[20]�'ä

,ù��

,�s

,°

.mnH-9k-5]Û%^_Û¹

、Z[\

3�Â�R���.ßà

[J].IJioBCCD

(��iCj

),2016,46(8):28-36.

[21]���

,����

,!J�

,°

.mnH-5]Û%^_��Û¹9Z

[\��.ßà

[J].��iÅ

,2013,38(1):17-20.[22]LEMOINEY,SCHOEFSB.Secondaryketocarotenoidastaxanthinbiosyn

thesisinalgae:Amultifunctionalresponsetostress[J].Photosynthesisresearch,2010,106(1/2):155-177.

[23]WAYAMAM,OTAS,MATSUURAH,etal.Threedimensionalultrastructuralstudyofoilandastaxanthinaccumulationduringencystmentinthegreenalga,Haematococcuspluvialis[J].PLoSOne,2013,8(1):1-9.

[24]BOROWITZKAMA,HUISMANJM,OSBORNA.CultureoftheastaxanthinproducinggreenalgaHaematococcuspluvialis1.Effectsofnutrientsongrowthandcelltype[J].Journalofappliedphycology,1991,3(4):295-304.

[25]öD�

,�Cí

,���

.���� ]Û%^_÷�Z[\.^_

[J].¡Û�C¢D

,2007,34(2):256-260.[26]MARKOUG,NERANTZISE.Microalgaeforhighvaluecompoundsand

biofuelsproduction:Areviewwithfocusoncultivationunderstressconditions[J].Biotechnologyadvances,2013,31(8):1532-1542.

[27]TOUCHETTEBW,BURKHOLDERJM.Reviewofnitrogenandphosphorusmetabolisminseagrasses[J].Journalofexperimentalmarinebiology&ecology,2000,250(1):133-167.

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