PATENT ABSTRACTS

permeability of 10, 000 to 50,000 I) in circulation against water, whereby conccntralion is con- ducted before andor after diaiittration.

4526782

P R O C E S S F O R R E C O V E R I N G I N T E R F E R O N

Yahiro Uemura, Hirofum Arimura, Itiroshi Morise, Satoshi Funakoshi, Tadakazu Suyama, Hirakata, Japan assigned to The Green Cross Corporation

Interferon can be recovered simply by con- tacting an aqueous solution cuntaming inter- feron which is induced and produced from cells of human origin capable of producing inter- feron, with a siliceous substance to adsorb inter- feron on it and eluting the adsorbed interferon with an aqueous solution containing a nonionic surfactant, preferably further containing an ac- riflavine such as acrinol.

4 5 ~ ! ~

M E T H O D O F S E P A R A T I N G A I 4 - 1 2 5 I - I N S U L I N F R O M

H E T E R O G E N E O U S L Y L A B E L E D I N S U L I N M O L E C U L E S F O R

B I O L O G I C A L S T U D I E S

Frankie B Stentz, Reba K Wright, Abbas E Kitabchi assigned to University of TN Research Corporation

~ cl~_uE~i~ SEp~ATtON OF" U ~ I , ~ D INSIU N

1 t i

i:

Method of separating A14-125I-insulin from heterogeneously labeled insulin molecules for biological studies which involves iodating in- sulin in a controlled manner, selectively abs- orbing the insulin from the iodination procedure in a tightly packed bed of octadecylsilane bon- ded to silica employing reverse phase liquid chromatography principles, thereafter eluting large and small radioactive and nonradioactive materials such as damaged insulin, various con- taminants and polar materials from the bed with

267

dilute trilluoroacetic acid, thereafter eluting the inlact 1251-insulin from thc bed with a mixture of acctonitrile and dilute t rifluoroacetic acid and thcn separating the Al4-insulin by high perlbr- mancc liquid chromatography or ion exchange chromatography.

4528186

M E T H O D O F P R O D U C I N G H U M A N E P I D E R M A L G R O W T H

F A C T O R

Toyohiko Nishimura, Naomi Uchida, Hajime Hiralani, Ashiya. Japan assigned to Japan Chemical Rcsearch Co Ltd

A method for producing a human epidermal growth factor in high yield and high purity by adding particles of an aluminum or magnesium silicate of the formula z.2 (or 6) SiO2.xH20 whcre Z is AI203 or 2MgO and x is the number of water molecules in each molecule of the com- pound, to human urine under neutral to acid conditions to adsorb the factor on the particles and then eluting the factor with an alkaline solu- tion.

4 5 ~ 1 ~

P O L Y S A C C H A R I D E P S - A O B T A I N E D F R O M B A R R E N W O R T

D E R I V I N G F R O M P L A N T S B E L O N G I N G T O T H E G E N U S E P I M E D I U M , P R O C E S S F O R

P R E P A R A T I O N T H E R E O F A N D P H Y L A C T I C A N D

I M M U N O S T I M U L A T I N G A G E N T S C O M P R I S I N G S A I D

P O L Y S A C C H A R I D E P S - A E F F E C T I V E C O M P O N E N T

Susumu Mitsuhashi, Muneaki Takase, Sosuke Yasui, Ichiro Wa~hizawa, Kimitomo Yoshioka, Tokyo, Japan assigned to Zenyaku Kogyo Kabushiki Kaisha

A polysaccharide PS-A obtained from plants belonging to the genus Epimedium and having the following physicochemical properties, a pro- cess for preparation thereof and use as phylactic and immunostimulating agents comprising the polysaccharide PS-A as an effective component: I. Elementary analysis: C=40.92; H=6.17; Ash = very small; 2. Molecular weight: 75,000 +/-25,000 (average molecular weight); 3. Decomposition point: 205 degrees C.; 4. pH: 7.0 (solution of 100 mg of PS-A in 50 ml of water); 5. Specific rotatory power: ( alpha)DI9=-23.6 degrees (in H20, c= 0.527); 6. Infrared absorp- tion spectrum: numaxKBr(cm-l)/3400, 2900, 1620, 1400, 1230, 1060; 7. Ultraviolet absorption