Technical Note

Analysis of Sugars in Honey and Syrup

Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.

Standards Honey

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: 1; Fructose (30mg/ml)2; Glucose (24mg/ml)3; Sucrose (6mg/ml)4; Maltose (6mg/ml)

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Honey (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Gum syrup Gum syrup (contains honey)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Gum syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Gum syrup (contains honey)(50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Maple syrup Sugar syrup (Maple flavor)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Maple syrup (50mg/ml)1; Fructose2; Glucose 3; Sucrose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Sugar syrup (Maple flavor)(50mg/ml)1; Fructose2; Glucose 3; Sucrose

Inj.Vol.: 5.0µl

1701

For research use only, not intended for diagnostic or drug use.

NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855 JAPANTELFAXWebsiteE-mail

: +81-(0)75-251-1730: +81-(0)75-251-1763: www.nacalai.com: info.intl@nacalai.com

Apple syrup Agave syrup

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Apple syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Agave syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Starch syrup Starch syrup (fast analysis)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 60/40Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose

Inj.Vol.: 5.0µl

More Sugar-D application data are available on our website (http://www.nacalai.co.jp/ cosmosil/data/csmosrchtop.cfm). Or you can search “COSMOSIL Application”.

DurabilityThe decrease of retention time was compared between COSMOSIL Sugar-D and conventional aminopropyl bonded stationaryphase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case to COSMOSIL Sugar-D.

[Test Condition]Column SizeMobile PhaseFlow RateTemperatureDetectionSample

4.6 mm I.D. x 250 mmAcetonitrile : Water = 70 : 301.0 ml/min30RIMaltose

[Eluent Condition]EluentFlow RateTemperature

Water1.0 ml/minRT

120%

0%

100%

80%

60%

40%

20%

0 12010080604020Time (hr)

Sugar-DAminopropyl Type