avaliação comparativa de teste imunocromatográfico

16

P. LONGO / REVISTA BRASILEIRA DE CRIMINALSTICA, V. 1, N.1, 16-21 (2011)

1. INTRODUO

$LGHQWLFDomRGHVDQJXHHGHVXDRULJHPKXPDQDem amostras provindas de investigaes criminais no

(VWDGRGH6mR3DXORWHPVLGRUHDOL]DGDQRVODERUDWyULRVGR1~FOHRGH%LRORJLDH%LRTXtPLFD1%%GR,QVWLWXWRGH &ULPLQDOtVWLFD ,& DWUDYpV GRV PpWRGRV EDVHDGRVQR UHDJHQWH .DVWOH0H\HU SDUD HQVDLRV SUHVXQWLYRVH QR WHVWH LQGLUHWR GH &RRPEV SDUD GHWHUPLQDomR GHHVSHFLFLGDGHKXPDQD

$OJXQV ODERUDWyULRV SROLFLDLV GD $OHPDQKD HGD 6XtoD XWLOL]DP R WHVWH LPXQRFURPDWRJUiFR SDUDLGHQWLFDomRGHVDQJXHKXPDQR(VVHVWHVWHVVHXWLOL]DPGHDQWLFRUSRDQWLKHPRJORELQDKXPDQDTXHDSOLFDGRVHPDPRVWUDVGHPDWHULDOELROyJLFRREWLGDVHPLQYHVWLJDomRFULPLQDOSRGHP LQGLFDUDSUHVHQoDGH VDQJXHKXPDQRmesmo em amostras antigas, contaminadas ou em estado

GHSXWUHIDomR >@$VVLPHVVHV WHVWHV WrPVHPRVWUDGRHFD]HV H UREXVWRV FRP JUDQGH DSOLFDomR j SHUtFLDFULPLQDO

$YDOLDomRFRPSDUDWLYDGHWHVWHLPXQRFURPDWRJUiFRSDUDLGHQWLFDomRIRUHQVHGHVDQJXHKXPDQR

3/RQJRa&5'LDV)LOKRE0329DODGDUHVc(&$ORQVR d636*RQoDOYHVe(Auler-Bittencourt e,*

a3HULWR&ULPLQDO1~FOHRGH3HUtFLDV&ULPLQDOtVWLFDVGH$UDUDTXDUD,QVWLWXWRGH&ULPLQDOtVWLFDGH6mR3DXOR%UDVLOE3HULWR&ULPLQDO(TXLSHGH3HUtFLDV&ULPLQDOtVWLFDVGH$PHULFDQD,QVWLWXWRGH&ULPLQDOtVWLFDGH6mR3DXOR%UDVLO

c 3HULWR&ULPLQDO1~FOHRGH3HUtFLDVHP&ULPHV&RQWUDD3HVVRD,QVWLWXWRGH&ULPLQDOtVWLFDGH6mR3DXOR%UDVLOd *UDGXDQGRHP&LrQFLDV%LROyJLFDVGR,QVWLWXWRGH%LRFLrQFLDV8QLYHUVLGDGHGH6mR3DXOR%UDVLO

e 3HULWR&ULPLQDO1~FOHRGH%LRORJLDH%LRTXtPLFD,QVWLWXWRGH&ULPLQDOtVWLFDGH6mR3DXOR%UDVLO(QGHUHoRGHHPDLOSDUDFRUUHVSRQGrQFLDHORLVDHDDE#SROLFLDFLHQWLFDVSJRYEU

5HFHELGRHP5HYLVDGRHP$FHLWRHP

RESUMO

$GHWHUPLQDomRGHHVSHFLFLGDGHKXPDQDSRGHLQGLFDUDSUHVHQoDGHVDQJXHKXPDQRPHVPRHPDPRVWUDVDQWLJDVSURYLQGDVGHLQYHVWLJDo}HVFULPLQDLVFRQWDPLQDGDVRXHPHVWDGRGHSXWUHIDomR2SUHVHQWHHVWXGRYLVDDQDOLVDUVHKiFRQFRUGkQFLDHQWUHRVUHVXOWDGRVREWLGRVSHORVWHVWHVGHRULHQWDomRHHVSHFtFRSDUDVDQJXHKXPDQRXWLOL]DGRVURWLQHLUDPHQWHQDDQiOLVHIRUHQVHHPFRPSDUDomRFRPRWHVWHLPXQRFURPDWRJUiFRSDUDGHWHFomRGDKHPRJORELQDKXPDQD2UHVXOWDGRDOFDQoDGRSRUPHLRGRDQWLFRUSRDQWLKHPRJORELQDKXPDQDPRVWURXVHHFLHQWHQDGHWHFomRTXDOLWDWLYDGHKHPRJORELQDKXPDQDHPDPRVWUDVGHVDQJXHGHSRVLWDGDVHPSHoDVHORFDLVGHFULPHPalavras-chave LGHQWLFDomR GH VDQJXH WHVWH LPXQRFURPDWRJUiFR DPRVWUDV ELRORJLFDV LGHQWLFDo}HV FULPLQDLV ELRORJLDIRUHQVH

ABSTRACT

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eywordsEORRGLGHQWLFDWLRQLPPXQRFKURPDWRJUDSKLFWHVWELRORJLFDOVDPSOHVFULPLQDOLQYHVWLJDWLRQVIRUHQVLFELRORJ\

%DVHDGR QR GLDJQyVWLFR SRU PHLR GR DQWLFRUSRDQWLKHPRJORELQDKXPDQDR)HFD&XOW2QH6WHS7HVWH>@ p XP WHVWH LPXQRFURPDWRJUiFR HP IRUPD GH WLUDRX GLVSRVLWLYR )LJ RULJLQDOPHQWH GHVHQYROYLGRSDUD D GHWHFomR GH KHPRJORELQD KXPDQD HP IH]HV$LQWHUSUHWDomRGRUHVXOWDGRpUiSLGDSRUPHLRGDSUHVHQoDGH FRU HP UHJL}HV HVSHFtFDV GD WLUD 6XD XWLOL]DomRIRUHQVHIRLSRVWHULRUPHQWHDGDSWDGDSRU+RFKPHLVWHUet al>@

2SUHVHQWHHVWXGRYLVDDQDOLVDUVHKiFRQFRUGkQFLDHQWUH RV UHVXOWDGRV REWLGRV SHORV WHVWHV .DVWOH0H\HU&RRPEV H R )HFD&XOW SDUD FRQVWDWDomR GD SUHVHQoDGHVDQJXHKXPDQRHPSHoDVFULPLQDLV%XVFD WDPEpPFRPSDUDU D VHQVLELOLGDGH GD GHWHUPLQDomR GH VDQJXHKXPDQR HP DPRVWUDV GLOXtGDV HPSUHJDQGRVH RVWHVWHV &RRPEV H )HFD&XOW H DYDOLDU VH Ki LQXrQFLDGD R[LGDomR GH PDWHULDLV TXH FRQVWLWXHP DV SHoDV GHH[DPHVVREUHRUHVXOWDGRGHVWHVWHVWHV

17


Figura 1HVTXHUGDIRUPDVGHDSUHVHQWDomRGR.LW)HFD&XOWWLUDVXSHULRU RX GLVSRVLWLYR LQIHULRU GLUHLWD WXER FROHWRU FRQWHQGRVXVSHQVmRGHSURWHtQDHFRQVHUYDQWHV

2. MATERIAL E MTODO

2.1. Material

2V WHVWHV IRUDP UHDOL]DGRV FRPR NLW )HFD&XOW2QH6WHS7HVWHQDIRUPDGHWLUDSURGX]LGRSRU$ODPDU7HFQR&LHQWtFD/WGD2NLWIRUQHFLGR)LJFRQWpPWLUDV GH WHVWH FRP UHDJHQWHV LPRELOL]DGRV HP XPDPHPEUDQDHPXPDSURWHtQDPDWUL]FRPD]LGDVyGLFDHWXERV FROHWRUHV FRQWHQGRXPD VXVSHQVmRGHSURWHtQD HFRQVHUYDQWHV>@3ULQFtSLRGRWHVWHLPXQRFURPDWRJUiFR

$ WLUD GR WHVWH FRQWpP XPD PHPEUDQD SUpLPSUHJQDGDFRPDQWLFRUSRPRQRFORQDODQWLKHPRJORELQDKXPDQDQDiUHDGDOLQKDWHVWHHDQWLFRUSRGHFDEUDDQWLFDPXQGRQJRQDiUHDGDOLQKDFRQWUROH8PDDOPRIDGDFRP DQWLFRUSR SROLFORQDO DQWLKHPRJORELQD KXPDQDFRQMXJDGR GH RXUR FRORLGDO p FRORFDGR QR QDO GDPHPEUDQD QD iUHD GH DSOLFDomR GD DPRVWUD 4XDQGRD KHPRJORELQD KXPDQD HVWLYHU SUHVHQWH QD DPRVWUDdissolvida na suspenso de protenas e conservantes, a

PLVWXUDGRFRQMXJDGRRXURFRORLGDOHDDPRVWUDH[WUDtGDPRYHPVHDRORQJRGDPHPEUDQDFURPDWRJUDFDPHQWHSRUDomRGHFDSLODULGDGH(VWDPLVWXUDHQWmRPLJUDSDUDD iUHD GD OLQKD WHVWH H IRUPDXPD OLQKD YLVtYHO FRPRFRPSOH[RGHDQWLFRUSRVFRPDKHPRJORELQDKXPDQD1DDXVrQFLDGDKHPRJORELQDKXPDQDQDDPRVWUDH[WUDtGDQmRKiIRUPDomRGHQHQKXPDIDL[DGHFRUYLVtYHOQDiUHDGDOLQKDWHVWH3RUWDQWRDSUHVHQoDGHXPDIDL[DGHFRUQDiUHDGDOLQKDWHVWHLQGLFDXPUHVXOWDGRSRVLWLYR)LJ8PDIDL[DFRORULGDGHYHUiVHPSUHDSDUHFHUQDiUHDGDOLQKDFRQWUROHSDUDYDOLGDomRGRWHVWH>@>@

Figura 2. ,QWHUSUHWDomR GR WHVWH$ SUHVHQoD GD OLQKD FRQWUROH &LQGLFD D YDOLGDGH GR WHVWH D UHDomR FRORULPpWULFD QD OLQKD WHVWH 7UHYHODUHVXOWDGRSRVLWLYRSDUDVDQJXHKXPDQR

2.3. Procedimento de coleta de amostras

$V DPRVWUDV SDUD WHVWH FRP R )HFD&XOW IRUDPREWLGDV XWLOL]DQGRVH WUrV SURFHGLPHQWRV GHQRPLQDGRV$%H&GHDFRUGRFRPRVSDUkPHWURVHVWDEHOHFLGRVFRQFHQWUDomRHTXDQWLGDGHGDDPRVWUD2SURFHGLPHQWR$ IRL XWLOL]DGR FRP DPRVWUDV PDLV FRQFHQWUDGDV HHP PDLRU TXDQWLGDGH H FRQVLVWLX HP HVIUHJDU D SDUWHHVWULDGDGDKDVWHGRDSOLFDGRU)LJVREUHDPDQFKD)LJ H LQWURGX]LU QR WXER FROHWRU DJLWDQGR R WXERYLJRURVDPHQWHGHPDQHLUDDDVVHJXUDUXPDERDGLIXVmRHKRPRJHQHL]DomRGDVROXomR2VSURFHGLPHQWRV%H&IRUDPXWLOL]DGRVFRPDPRVWUDVPHQRVFRQFHQWUDGDVH HPPHQRUTXDQWLGDGH %FRQVLVWLX HP UHFRUWDUXPIUDJPHQWR GD DPRVWUD H LQWURGX]LU QR RULItFLR GR WXERFROHWRUSDUDDH[WUDomRGRPDWHULDOFRPDVROXomR)LJ(P&XPSHGDoRGDDPRVWUDpFRORFDGRHPXPWXERGHHQVDLRMXQWRFRPRFRQWH~GRGHVROXomRGHWXERFROHWRU)LJ2WXERpDJLWDGRHGHL[DVHH[WUDLQGRSRUDOJXQVVHJXQGRV

Figura 3.,OXVWUDomRGRWXERFROHWRUGR)HFD&XOWFRQWHQGRXPDKDVWHDSOLFDGRUDLPHUVDHPVXVSHQVmRGHSURWHtQDVHFRQVHUYDQWHV>@

$SyV D H[WUDomR GDV DPRVWUDV HP $ H %D H[WUHPLGDGH GR WXER FROHWRU IRL URPSLGD H IRUDPGLVSHQVDGDVJRWDV QD H[WUHPLGDGH DEVRUYHQWHGD WLUD)LJ1R SURFHGLPHQWR & D VROXomR GD DPRVWUDH[WUDtGDQRWXERGHHQVDLRIRLJRWHMDGDYH]HVVREUHDH[WUHPLGDGH DEVRUYHQWH GD WLUD FRP R DX[tOLR GH XPDSLSHWD2UHVXOWDGRIRLOLGRHPPLQXWRV

Figura 4. 3URFHGLPHQWR$HVIUHJDVHDSDUWHHVWULDGDGDKDVWHGRDSOLFDGRUVREUHDPDQFKD

18

Figura 5. 3URFHGLPHQWR % LQWURGX]VH XP IUDJPHQWR GD DPRVWUDSHORRULItFLRGRWXERFROHWRU

Figura 6.3URFHGLPHQWR&XPSHGDoRGDDPRVWUDpFRORFDGRHPXPWXERGHHQVDLRMXQWRFRPRFRQWH~GRGHVROXomRGHWXERFROHWRU

Figura 7.$SyVPDFHUDGDDDPRVWUDQRVSURFHGLPHQWRVGLVSHQVDPVHJRWDVQDH[WUHPLGDGHDEVRUYHQWHGDWLUD7HVWHVGHLGHQWLFDomRGHVDQJXHHGHHVSHFLFLGDGHhumana

$ KHPRJORELQD p XPD SURWHtQD FRQMXJDGD TXHHVWiSUHVHQWHQRVJOyEXORVYHUPHOKRVGR VDQJXHH WHPSDSHO IXQGDPHQWDO QHVVHV WHVWHV e FRQVWLWXtGD SHODSURWHtQDJORELQDHSHORJUXSRKHPH>@

2 UHDJHQWH GH .DVWOH0H\HU IXQFLRQD FRPRLQGLFDGRU DSyV D DGLomR GH SHUy[LGR GH KLGURJrQLR jDPRVWUD HP VROXomR VLROyJLFD 2 WHVWH p EDVHDGR QDDWLYLGDGH FDWDOtWLFD GR KHPH GD KHPRJORELQD TXH DWXDVREUHRSHUy[LGRHOLEHUDR[LJrQLRTXHR[LGDRLQGLFDGRUGR UHDJHQWH IHQROIWDOHtQD GDQGR FRORUDomR URVD jVROXomR2WHVWHSRGHIRUQHFHUUHVXOWDGRVIDOVRSRVLWLYRVFRPRXWUDVVXEVWkQFLDVPDVUHDo}HVQHJDWLYDVVmRSURYDGDDXVrQFLDRXGHTXDQWLGDGHVQmRGHWHFWiYHLVGHKHPH>@ 2V WUDEDOKRV FRQVXOWDGRV DSUHVHQWDUDP GLIHUHQWHVVHQVLELOLGDGHVSDUDRUHDJHQWHH>@>@1RSUHVHQWHWUDEDOKRDSHQDVDVDPRVWUDVSRVLWLYDV

FRPRWHVWHGH.DVWOH0H\HUIRUDPWDPEpPDQDOLVDGDVSHORWHVWHGH&RRPEV>@

2 WHVWH VRUROyJLFR GH &RRPEV RX 7HVWH GD$QWLJOREXOLQD +XPDQD LGHQWLFD D SUHVHQoD GH XPDSURWHtQDSUHVHQWHQRVRURGRVDQJXHKXPDQRQDVXSHUItFLHGRVJOyEXORVYHUPHOKRVDJOREXOLQD(VVHWHVWHGHWHFWDanticorpos incompletos de grupos sanguneos no soro

WHVWHLQGLUHWRGDDQWLJOREXOLQD$QWLFRUSRVLQFRPSOHWRVVmRDTXHOHVTXHIDOKDPHPDJOXWLQDUJOyEXORVYHUPHOKRVVXVSHQVRVHPVDOLQD1DUHDomRGDDQWLJOREXOLQDJOyEXORVYHUPHOKRV HQYROYLGRV FRP DQWLFRUSRV LQFRPSOHWRVFRPRDQWL5K,J*VmRDJOXWLQDGRVSRUDQWL,J*RTXDOOLJDDVPROpFXODVGH,J*DWUDYpVGHSRQWHVDRVJOyEXORVYHUPHOKRVYL]LQKRV2 IXQGDPHQWRGRPpWRGRpTXHDJOREXOLQDKXPDQDLQLEHDDWLYLGDGHGRVRURDQWLJOREXOLQDKXPDQRRTXDOSHUGHDFDSDFLGDGHGHDJOXWLQDUJOyEXORVYHUPHOKRV VHQVLELOL]DGRV SRU DQWLFRUSRV LQFRPSOHWRV6H XPDPDQFKD GH VDQJXH FRQWpP JOREXOLQDV GR VRURKXPDQRHpLQFXEDGDFRPDQWLVRURJOREXOLQDKXPDQRHVVDV JOREXOLQDV QHXWUDOL]DP R DQWLVRUR H TXDQGR VmRDGLFLRQDGDV FpOXODV 2 5KR' IRUQHFLGDV QR NLWGR WHVWH VRUROyJLFR GH &RRPEV VHQVLELOL]DGDV FRPDQWLFRUSRV LQFRPSOHWRV QHQKXPD DJOXWLQDomR RFRUUHVHQGRDPDQFKDGHVDQJXHSRUWDQWRGHRULJHPKXPDQD$ IRUPDomR GH DJOXWLQDomR VLJQLFD TXH DPDQFKD GHVDQJXH QmR FRQWpP JOREXOLQD KXPDQD H TXH R DQWLVRUR JOREXOLQD KXPDQRFRX OLYUH SDUD IRUPDU SRQWHVDJOXWLQDQGR RV JOyEXORV YHUPHOKRV VHQVLELOL]DGRV $VHQVLELOLGDGHGRWHVWHpGDRUGHPGH>@$YDOLDomRGDVHQVLELOLGDGHGRVWHVWHVSDUDGHWHFomRde sangue humano

&ROHWDGRPDWHULDOSDUDGHWHUPLQDomRGHVHQVLELlidade dos testes

)RUDP FROHWDGRV P/ GH VDQJXH KXPDQR SRUpuno venosa para serem empregados nas diluies,

com especial ateno para a medio dos volumes

empregados nos procedimentos antes da coagulao

'LOXHQWHVHPSUHJDGRV$V VHTrQFLDV GH GLOXLomR IRUDP HIHWXDGDV

GLVWLQWDPHQWH FRP iJXD GHVWLODGD VROXomR VLROyJLFD1D&OHVROXomRVDOLQDWDPSRQDGDGRWXERFROHWRUGR)HFD&XOW0pWRGRHPSUHJDGR

)RLWUDQVIHULGRP/GHVDQJXHKXPDQRLQQDWXUDSDUDFDGDXPGRVWUrVUHFLSLHQWHVFRQWHQGRRVGLOXHQWHVHVSHFLFDGRVjWHPSHUDWXUDDPELHQWHDSDUWLUGRVTXDLVIRUDPHIHWXDGDVDVGLOXLo}HVGH H2WHVWHGH&RRPEVIRLDSOLFDGRSDUDDVGLOXLo}HVGHDHQTXDQWRR)HFD


19

&XOWIRLDSOLFDGRHPWRGDVDVGLOXLo}HV$YDOLDomRGDLQXrQFLDGHR[LGDomRGHSHoDVVREUHRUHVXOWDGRGRVWHVWHVGH&RRPEVH)HFD&XOW

)RUDP DQDOLVDGDV OkPLQDV GH DoR R[LGDGDV HQmR R[LGDGDV HQFDPLQKDGDV DR 1~FOHR GH 3HUtFLDV&ULPLQDOtVWLFDV 13& GH$UDUDTXDUD SDUD SHVTXLVD GHVDQJXHKXPDQRFRPSDUDQGRVHRVUHVXOWDGRVGRVWHVWHVGH&RRPEVH)HFD&XOW3. RESULTADOS E DISCUSSO

3.1. Procedimento de coleta de amostras

8P WRWDO GH DPRVWUDV IRUDP DQDOLVDGDVDWUDYpVGRVSURFHGLPHQWRV$%H&2SURFHGLPHQWR$PRVWURXVH SUiWLFR SRUpP EDVWDQWH OLPLWDGR 3{GH VHUXVDGRTXDQGRKRXYHPDQFKDVKHPDWyLGHVFRQFHQWUDGDVHP REMHWRV UtJLGRV WDLV FRPR IDFD GH PRGR TXH DVXEVWkQFLD KHPiWLFD VH DGHULD IDFLOPHQWH jV HVWULDV GDKDVWH GR WXER FROHWRU )RL XWLOL]DGR HP DPRVWUDVGRV WHVWHV -iRSURFHGLPHQWR%VHPRVWURXSRXFRSUiWLFRGHYLGRDRRULItFLRGRWXERFROHWRUSRVVXLUGLkPHWURGHFHUFDGHPPRTXHGLFXOWDD LQVHUomRGHXPIUDJPHQWRGHDPRVWUDPDLRUVHQGRXWLOL]DGRHPDSHQDVDPRVWUDVGRVWHVWHV2SURFHGLPHQWR& DSUHVHQWRXVH UHODWLYDPHQWH SUiWLFR H VHUYLX SDUDTXDOTXHUFRQFHQWUDomRGHDPRVWUD)RLRPDLVXWLOL]DGRVHQGRHPSUHJDGRHPDPRVWUDV &RPSDUDomR HQWUH RV WHVWHV GH .DVWOH0H\HU GH&RRPEVH)HFD&XOW

$ 7DEHOD H[SUHVVD RV UHVXOWDGRV GRV WHVWHVGH &RRPEV H )HFD&XOW 2V UHVXOWDGRV SDUD &RRPEV)HFD&XOW15H TXHHTXLYDOHPDGRVUHVXOWDGRVLQGLFDUDPFRQFRUGkQFLDHQWUHRVWHVWHV

Tabela 1. 5HVXOWDGRGRVWHVWHVGH.DVWOH0H\HUGH&RRPEVH)HFD&XOWGDVDPRVWUDVDQDOLVDGDVQ

Kastle-Meyer Coombs Feca-Cult No de amostra

- 15 - - - - - - *

SRVLWLYRQHJDWLYR15QmRUHDOL]DGR

UHDOL]DGRQDWHQWDWLYDGHVHHVFODUHFHURUHVXOWDGRGLYHUJHQWH

$SHQDVDPRVWUDVLQGLFDUDPGLYHUJrQFLDHQWUH&RRPEVHR)HFD&XOW7HVWHVLPXQRFURPDWRJUiFRVSDUD HVSHFLFLGDGH KXPDQD FRPR R )HFD&XOWapresentam resultado positivo para sangue total diludo

DWp1RWHVWHGH&RRPEVUHVXOWDGRVSRVLWLYRVVmRREWLGRV FRP VDQJXH WRWDO GLOXtGR DWp >@/RJRR)HFD&XOWpYLQWHYH]HVPDLVVHQVtYHOTXHRWHVWHGH&RRPEVQD LGHQWLFDomRGH VDQJXHKXPDQR7RGDV

DV DPRVWUDV TXH WLYHUDP UHVXOWDGRV GLYHUJHQWHV HQWUHR )HFD&XOW H R &RRPEV IRUDP REWLGDV GH SHoDV FRPVXEVWkQFLDKHPDWyLGHHPEDL[DTXDQWLGDGHRTXHSRGHWHUVLGRGHWHFWDGRSHORVWHVWHVGH.DVWOH0H\HUH)HFD&XOWPDVQmRSHORWHVWHGH&RRPEVGHYLGRDVXDPHQRUVHQVLELOLGDGH

2 UHVXOWDGR QHJDWLYR SDUD RPpWRGR GH.DVWOH0H\HU IRL REWLGR HP DSHQDV XPD DPRVWUD GH XPDIDFD TXH SDUWLFXODUPHQWH VH HQFRQWUDYD HP HVWDGRDYDQoDGR GH R[LGDomR 2 UHVXOWDGR SRVLWLYR SDUD R)HFD&XOW LQGLFRX SUHVHQoD GH KHPRJORELQD KXPDQD>@1RHQWDQWRRPpWRGRGH.DVWOH0H\HUTXHUHVXOWDSRVLWLYR TXDQGR GD SUHVHQoD GH KHPRJORELQD >@ QmRFRQVWDWRXDSUHVHQoDGHVDQJXH(VVDGLYHUJrQFLDSRGHVHUH[SOLFDGDSHODVHQVLELOLGDGHYDULiYHOGRUHDJHQWHGH.DVWOH0H\HU'LIHUHQWHV VHQVLELOLGDGHVSDUDRPpWRGRIRUDP FLWDGDV SRU GLIHUHQWHV DXWRUHV GLIHULQGR HP DWpPLO YH]HV >@ >@$VVLP R UHDJHQWH GH.DVWOH0H\HUSRGHWHUVHQVLELOLGDGHLQIHULRUjTXHODDWULEXtGDDR)HFD&XOW 2XWUD SRVVLELOLGDGH VHULD GH TXH R HOHYDGR JUDXGH R[LGDomR GD SHoD SRGH WHU VXSULPLGR D FDSDFLGDGHGD KHPRJORELQD GH UHGX]LU R SHUy[LGR GH KLGURJrQLRQR PpWRGR GH .DVWOH0H\HU 2 WHVWH GH &RRPEV IRLH[FHSFLRQDOPHQWH UHDOL]DGR QHVVD DPRVWUD QHJDWLYDSDUD VDQJXH JHQpULFR PDV UHVXOWRX QHJDWLYR R TXHHUD HVSHUDGR GHYLGR VXD PHQRU VHQVLELOLGDGH TXDQGRFRPSDUDGRDR.DVWOH0H\HU&RPSDUDomRGDVHQVLELOLGDGHGRVWHVWHVGHGHWHFomRde sangue humano

2V UHVXOWDGRV REWLGRV FRQIRUPH FDGDconcentrao e os diluentes empregados encontram-se

QDV7DEH2V UHVXOWDGRV H[SRVWRV QD 7DE LOXVWUDGRV

GD )LJ DSRQWDUDP D GHWHFomR GH VDQJXH KXPDQRQDVVROXo}HVGHFRQFHQWUDomRDWpHPSUHJDQGRVH R WHVWH GH &RRPEV WHQGRVH FRPR VROYHQWH DVROXomRVLROyJLFD H D VROXomR VDOLQD WDPSRQDGD3DUDDV FRQFHQWUDo}HV GH VDQJXH GH D RV UHVXOWDGRV IRUDP QHJDWLYRV REVHUYDQGRVH QRPLFURVFySLRySWLFRDVDJOXWLQDo}HVFDUDFWHUtVWLFDVGHVWHVUHVXOWDGRVQHJDWLYRV

3DUDDVDPRVWUDVGLOXtGDVHPiJXDGHVWLODGDWRGRVRV WXERV DSUHVHQWDUDP DJOXWLQDo}HV FDUDFWHUL]DQGRRV UHVXOWDGRV QHJDWLYRV SDUD R PpWRGR VRUROyJLFRHPSUHJDGR$VVLP SDUD DV FRQFHQWUDo}HV GLOXtGDV GHVDQJXH KXPDQR D KHPyOLVH GDV KHPiFLDV RFDVLRQDGDSHOD iJXD GHVWLODGD LPSRVVLELOLWRX VXD DJOXWLQDomRJHUDQGR UHVXOWDGRV IDOVRQHJDWLYRV HP FRQFHQWUDo}HVTXH GHYHULDP VHU GHWHFWDGDV SHOR PpWRGR VRUROyJLFR3RUWDQWRSDUDTXHKDMDDSUHVHUYDomRGRVDQJXHDFROHWDGDVDPRVWUDVHPORFDLVGHFULPHGHYHVHUHIHWXDGDFRPVROXomRVLROyJLFDRXVDOLQDWDPSRQDGD

2 WHVWH )HFD&XOW DSRQWRX UHVXOWDGRV SRVLWLYRVQDVFRQFHQWUDo}HVGHDQmRGHWHFWDGDVSHOR PpWRGR VRUROyJLFR HYLGHQFLDQGR VXD PDLRUVHQVLELOLGDGH)LJ


20

Figura 8. ,OXVWUDomR GRV UHVXOWDGRV SRVLWLYRV DSRQWDGRV QD 7DEREWLGRV SHOR PpWRGR GH PHPEUDQD UHDWLYD )HFD&XOW 'D HVTXHUGDSDUD D GLUHLWD RV UHVXOWDGRV UHIHUHPVH jV FRQFHQWUDo}HV GH D

1D 7DEHOD IRUDP DSRQWDGRV RV UHVXOWDGRVSURYHQLHQWHV GRPpWRGR )HFD&XOW SDUD FRQFHQWUDo}HVDFLPD GH YH]HV GH GLOXLomR 2V UHVXOWDGRVREWLGRV DSRQWDUDP SDUD D GHWHFomR GH VDQJXH KXPDQRHP FRQFHQWUDo}HV H[WUHPDPHQWH UHGX]LGDV TXDQGRFRPSDUDGRV DRV UHVXOWDGRV REWLGRV SHOR PpWRGRVRUROyJLFR DWXDOPHQWH HPSUHJDGR SHORV 1~FOHRV GH3HUtFLDV&ULPLQDOtVWLFDV

Tabela 3.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPGLIHUHQWHVGLOXHQWHVHFRQFHQWUDo}HViJXDGHVWLODGDVROXomRVLROyJLFDHVDOLQD

WDPSRQDGDHPSUHJDQGRVHR)HFD&XOW

Concent.Feca- Cult

gua destiladaSoluo

VLROyJLFDSalina

tamponada - - - -

SRVLWLYRQHJDWLYR

$V DPRVWUDV TXH FRQWLQKDP R VDQJXH KXPDQRGLOXtGR HP VROXomR VLROyJLFD UHYHODUDP UHVXOWDGRVSRVLWLYRV DWp D FRQFHQWUDomR GH (VWHV

UHVXOWDGRV WDPEpP IRUDP REVHUYDGRV QDV DPRVWUDVGLOXtGDV FRP VROXomR VDOLQD WDPSRQDGD IRUQHFLGD FRPR NLW 5HVVDOWDVH TXH D IDL[D SRVLWLYD GD PHPEUDQDrevelou-se mais intensa na diluio empregando-se a

VDOLQDHPFRPSDUDomRjVROXomRVLROyJLFD)LJ

Figura 9. 0HPEUDQDV UHDWLYDV )HFD&XOW UHVXOWDQWHV GD DQiOLVHGH VROXo}HV GH VDQJXH KXPDQR FRP FRQFHQWUDomR GH GLOXtGDVHPiJXDGHVWLODGDVROXomRVLROyJLFDHVROXomRVDOLQDWDPSRQDGD

2VPHOKRUHVUHVXOWDGRVIRUDPREWLGRVQDVOHLWXUDVGDV PHPEUDQDV UHDWLYDV HIHWXDGDV FRP DV DPRVWUDVGLOXtGDV HP iJXD GHVWLODGD RQGH VH REWHYH UHVXOWDGRSRVLWLYR SDUD VDQJXH KXPDQR QD FRQFHQWUDomR GH

$ OLVH GDV KHPiFLDV SHOD iJXD GHVWLODGD SRGHWHU DX[LOLDGR QD GHWHFomR GH VDQJXH KXPDQR HPFRQFHQWUDo}HV DEDL[R GH GLVSRQLELOL]DQGRPDLV KHPRJORELQD QD VROXomR GHYLGR j UXSWXUD GRVHULWUyFLWRVTXDQGRFRPSDUDGDFRPDVVROXo}HVFRQWHQGRKHPRJORELQDV tQWHJUDV GDV GLOXLo}HV HIHWXDGDV FRP DVRXWUDVGXDVVROXo}HVWHVWDGDV

&RQIRUPH )LJ QRWDVH TXH D LQWHQVLGDGHGDV FRORUDo}HV GD WD WHVWH p PXLWR VHPHOKDQWH SDUDWRGRV RV VROYHQWHV XWLOL]DGRV QD GLOXLomR DLQGD TXHD FRQFHQWUDomR GD VROXomR FRP iJXD GHVWLODGD VHMD DPHWDGHGDVGHPDLV$YDOLDomRGDLQXrQFLDGHR[LGDomRGHSHoDVVREUHRUHVXOWDGRGRVWHVWHVGH&RRPEVH)HFD&XOW

2VUHVXOWDGRVGRVWHVWHVGH&RRPEVH)HFD&XOWGD DQiOLVH GH LQXrQFLD GH R[LGDomR VREUH R UHVXOWDGRGHVWHVWHVWHVHVWmRH[SUHVVRVQD7DE

Concentraogua destilada 6ROXomR)LVLROyJLFD Salina Tamponada

Coombs Feca- Cult Coombs Feca- Cult Coombs Feca- Cult

- - - - - - - - - - - - -

Tabela 2.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPSUHJDQGRVHFRPRGLOXHQWHiJXDGHVWLODGDVROXomRVLROyJLFDHVDOLQDWDPSRQDGDFRPSDUDQGRVHRVWHVWHVGH&RRPEVH)HFD&XOW

SRVLWLYRQHJDWLYR


21

Tabela 4.5HVXOWDGRVGDGHWHFomRGHVDQJXHKXPDQRHPDPRVWUDVR[LGDGDVHQmRR[LGDGDV

Pea Coombs Feca-Cult

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD -

/kPLQDGHDoRR[LGDGD

/kPLQDGHDoRR[LGDGD - -

/kPLQDGHDoRQmRR[LGDGD

/kPLQDGHDoRQmRR[LGDGD

/kPLQDGHDoRQmRR[LGDGD - -

/kPLQDGHDoRQmRR[LGDGD - -SRVLWLYRQHJDWLYR

2EVHUYDVHFRQFRUGkQFLDGHQ HQWUHRVWHVWHVGH&RRPEVH)HFD&XOW$H[FHomRVHGiQDOkPLQDR[LGDGDFXMRUHVXOWDGRGR)HFD&XOWVHUHYHORXHPWUrVminutos, mesmo tempo de revelao das amostras de

VDQJXHKXPDQRGLOXtGRHQWUHH,VVR SRGH H[SOLFDU R UHVXOWDGR QHJDWLYR GR WHVWH GH&RRPEVSDUDHVWD OkPLQDFRQVLGHUDQGRDGLIHUHQoDGHVHQVLELOLGDGH GHVWH PpWRGR TXDQGR FRPSDUDGR FRPR)HFD&XOW pSURYiYHOTXH D FRQFHQWUDomRGH VDQJXHIRVVH LQIHULRU j GHWHFWiYHO SRU&RRPEVPDV DFLPDGROLPLWHGHGHWHFomRSHOR)HFD&XOW4. CONCLUSES

2)HFD&XOW DSUHVHQWRXFRQFRUGkQFLDGHFRPRVUHVXOWDGRVREWLGRVFRPDPpWRGRSDUDGHWHFomRGHVDQJXHXWLOL]DGRURWLQHLUDPHQWHHPDQiOLVHVIRUHQVHV3RGHVHDLQGDVXJHULUTXHIRLPDLVHFD]LGHQWLFDQGRDSDUHQWHPHQWH VDQJXH KXPDQR HP FRQFHQWUDo}HV QmRGHWHFWiYHLVSHORVRXWURVPpWRGRV

'HYHVH DQDOLVDU D FRQFHQWUDomR GD VXEVWkQFLDKHPiWLFD SDUD VH HVWDEHOHFHU TXDO R VROYHQWH PDLVDGHTXDGR SDUD FDGD VLWXDomR (P FRQFHQWUDo}HV H

TXDQWLGDGHVPXLWREDL[DVGHVXEVWkQFLDRXVRGDiJXDGHVWLODGD IRUQHFH UHVXOWDGRVPDLV VHQVtYHLV FRP R XVRGR.LW)HFD&XOWDSHVDUGHDSUHVHQWDU UHVXOWDGR IDOVRQHJDWLYR QR H[DPH VRUROyJLFR DWUDYpV GR PpWRGR GH&RRPEV-iDVROXomRVLROyJLFDHDVDOLQD WDPSRQDGDSRGHPVHUXWLOL]DGDVSDUDDVGHPDLVFRQFHQWUDo}HVHPDPERVRVWHVWHV

2DoRR[LGDGRSDUHFHQmRDIHWDUR UHVXOWDGRGR)HFD&XOW R TXH QRV VXJHUH TXH R UHVXOWDGR QHJDWLYRSDUD.DVWOH0H\HUHSRVLWLYRSDUD)HFD&XOWREVHUYDGRQD FRPSDUDomR HQWUH RV WUrV WHVWHV GHYH VHU H[SOLFDGRSHODKLSyWHVHPDLVSDUFLPRQLRVDOHYDQWDGDEDVHDGDQDSRVVtYHOGLIHUHQoDGHVHQVLELOLGDGHH[LVWHQWHHQWUHHVWHVWHVWHV

'HVWH PRGR D DQiOLVH HP DPRVWUDV VXVSHLWDVde sangue depositadas em peas criminais por meio do

DQWLFRUSRDQWLKHPRJORELQDKXPDQDPRVWURXVHHFLHQWHQD GHWHFomR TXDOLWDWLYD GH KHPRJORELQD KXPDQD9DOHapontar como vantagem em relao aos demais testes

HVSHFtFRVSDUDVDQJXHKXPDQRDUDSLGH]GRUHVXOWDGRHDIDFLOLGDGHGHDSOLFDomRGRWHVWHLPXQRFURPDWRJUiFRAGRADECIMENTOS

$JUDGHFHPRVDFRODERUDomRWpFQLFDGRVVHJXLQWHV3HULWRVFULPLQDLVGR1~FOHRGH%LRORJLDH%LRTXtPLFD+HOHQD0DULD )HUUHLUD &DVWUR ,RQH GH )UHLWDV$UDXMR5LFDUGR /RSHV 2UWHJD 5RVHO\ *DOR 6RDUHV 6RQLD5HJLQD (XJrQLR 0D]]RQL 0DULD $SDUHFLGD $YHOLQR0DULD/XLVD$OPHLGD3UDGR2OLYHLUD H6RXVD5REHUWD+LUVFKIHOG &DPSRORQJR H HP HVSHFLDO j WpFQLFD GHODERUDWyULR6XHOL)HOL[GRV6DQWRV2OLYHLUD

REFERNCIAS BIBLIOGRFICAS

>@01+RFKPHLVWHU%%XGRZOH56SDUNHV25XGLQ&*HKULJ07KDOL/6FKPLGW$&RUGLHU5'LUQKRIHU9DOLGDWLRQVWXGLHVRIDQLPPXQRFKURPDWRJUDSKLFVWHSWHVW IRU WKH IRUHQVLF LGHQWLFDWLRQ RI KXPDQ EORRG -)RUHQVLF6FL>@ )HFD&XOW2QH 6WHS7HVWH0HPEUDQD UHDWLYD SDUDGHWHUPLQDomRGHVDQJXHRFXOWR'LDGHPD$ODPDU7HFQR&LHQWtFD/WGD%XODGRWHVWHDEU>@0&7 6DZD\D0565ROLP0DQXDO SUiWLFR GHPHGLFLQDOHJDOQRODERUDWyULR-XUXi%UDVLOS>@'%'XSUp%ORRGRU7DFR6DXFH"-&KHP(GXF


Documents

avaliação comparativa de teste imunocromatográfico